Protective Effect of Heme Oxygenase-1 on High Glucose-Induced Pancreatic β-Cell Injury

Lee, Eun-Mi,Lee, Young-Eun,Lee, Esder,Ryu, Gyeong Ryul,Ko, Seung-Hyun,Moon, Sung-Dae,Song, Ki-Ho,Ahn, Yu-Bae Korean Diabetes Association 2011 Diabetes and Metabolism Journal Vol.35 No.5

<P><B>Background</B></P><P>Glucose toxicity that is caused by chronic exposure to a high glucose concentration leads to islet dysfunction and induces apoptosis in pancreatic β-cells. Heme oxygenase-1 (HO-1) has been identified as an anti-apoptotic and cytoprotective gene. The purpose of this study is to investigate whether HO-1 up-regulation when using metalloprotophyrin (cobalt protoporphyrin, CoPP) could protect pancreatic β-cells from high glucose-induced apoptosis.</P><P><B>Methods</B></P><P>Reverse transcription-polymerase chain reaction was performed to analyze the CoPP-induced mRNA expression of HO-1. Cell viability of INS-1 cells cultured in the presence of CoPP was examined by acridine orange/propidium iodide staining. The generation of intracellular reactive oxygen species (ROS) was measured using flow cytometry. Glucose stimulated insulin secretion (GSIS) was determined following incubation with CoPP in different glucose concentrations.</P><P><B>Results</B></P><P>CoPP increased HO-1 mRNA expression in both a dose- and time-dependent manner. Overexpression of HO-1 inhibited caspase-3, and the number of dead cells in the presence of CoPP was significantly decreased when exposed to high glucose conditions (HG). CoPP also decreased the generation of intracellular ROS by 50% during 72 hours of culture with HG. However, decreased GSIS was not recovered even in the presence of CoPP.</P><P><B>Conclusion</B></P><P>Our data suggest that CoPP-induced HO-1 up-regulation results in protection from high glucose-induced apoptosis in INS-1 cells; however, glucose stimulated insulin secretion is not restored.</P>

Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122의 생산과 그 특성

최용석,옥승호,유주현,배동현 한국산업미생물학회 1997 한국미생물·생명공학회지 Vol.25 No.1

토양으로부터 분리한 Metarrhizium anisopliae (Metschn.) Sorok이 생산하는 biopolymer를 정제하여 분석하고, 그의 생산조건을 검토하였다. 탄소원으로 mannitol, 질소원으로는 polypepton을 사용했을 때 가장 많은 biopolymer를 생산하였고 K_2HPO_4 및 CaCl_2를 첨가함으로서 생산량이 더욱 증가하였다. 또한 이 biopolymer를 정제하여 그 분자량과 구성성분을 검토한 결과 Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122는 분자량이 1.7×10 exp (6)이고, C, H의 구성비가 1:2이며 미량의 N이 검출되었으나, 주 구성성분은 glucose와 galactose의 환원당으로 이루어져있는 전혀 새로운 biopolymer인 것으로 나타났다. To produce biopolymer, Metarrhizium anisopliae (Metschn.) Sorok was cultured in a medium containing glucose 1.0%, sucrose 2.0%, soluble starch 1.0%, yeast extract 0.5%, polypeptone 0.5%, K_2HPO_4 0.1%, MgSO_4·7H_2O 0.02%. The culture broth was centrifuged and the polymer was harvested by adding methanol to the culture supernatant. When three times of methanol was added, the polymer was coagulated and precipitated. Then it was further purifued through successive SK-1B, SA-20P, HP-20 column chromatographies. This polymer was designated as Biopolymer YU-122. C:H ratio of this Biopolymer YU-122 was 1:2 and small amount of N is detected by CHN analyzer. Glucose and galactose are main components of this polymer. Average molecular weight of this biopolymer was 1.7×10 exp (6) by Sepharose 4B gel permeation chromatography. Optimal condition for biopolymer production was investigated. When 5% of mannitol was used as a carbon source, and polypepton as a N source, highest productivity of biopolymer was achieved. C/N ratio as nutrient was also a major factor in polymer production and its optimal ratio was 3.

심한 저칼륨혈증을 동반한 갑상선 중독성 주기성 마비 2례

유승진,최승필,최경호,오동렬,황주일,나병호,김세경 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

Thyrotoxic periodic paralysis occurs primarily in Orientals with an overwhelming male preponderance and a higher association of specific HLA antigens. Clinical manifestations include onset after high carbohydrate ingestion or heavy exertion with progressive symmetric weakness leading to flaccid paralysis of the extremities and other muscle groups, lasting several hours. Other precipitating facters include trauma, infection, administration of epinephrine, thyroid hormone, or corticosteroid. If hypokalemia is present, potassium administration may help abort the attack. Although propranolol can be efficacious in preventing further episodes, the only definitive treatment is euthyroid state. The pathophysiology is still controversal, but reflects altered potassium and calcium dynamics as well as certain morphologic characteristics within the muscle unit itself. We discribed two patients with thyrotoxic periodic paralysis who had a no familial history of paralysis and had a decreased level of serum potassium during attack.

특수가공용 공작기계의 쇼트피닝기 모델설계에 관한 연구

신용승,이승호,서유원,정성교 서울産業大學校 1999 논문집 Vol.49 No.2

As developing the technology in industry, the materials with superior grade of stiffiness and lightness are required, and hence the higher fatigue strength characteristics are necessary. Thus, the non-traditional machining to increase fatigue strength is becoming influential as very important technology. Considering environmental problem, the shot peening process is selected as a good process to increase fatigue strength. But the data related to shot peening process are not enough in this country. Shot peening process is essential to ensure repeatability and uniformity on a part to part and lot to lot basis, while conforming to the applicable specifications. The shot material, size, shape and hardness as well as velocity and impact angle must be controlled to provide consistency in peening results. In order to effectively control the shot peening process, parameters must be addressed in the following order, such as media control, coverage control, intensity control, equipment control and so on.

장기간 트레이닝이 내당능에 미치는 효과 분석

김승영,이배익,유관호 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.4

The present study was carried out to investigate the effects of physical training on glucose tolerance, insulin secretion, insulin sensitivity and insulin binding to erythrocytes in non-insulin dependent diabetic subjects(NIDDM) with fasting hyperglycemia and low insulin response during OGTT. The subjects were separated into two groups. One was diabetics treated with mild caloric restriction and physical training(Training group, n=18), the other was diabetics followed with mild caloric restriction alone(Sedentary group, n=20). Physical training consisted of 3-5 days weekly, 30 minutes sessions, with jogging corresponding to 40-50%VO₂max. Insulin sensitivity was determined by euglycemic insulin clamp technique. Following results were obtained. 1) Oral glucose tolerance was improved after 2 month training and remained unchanged thereafter in the training group. It was also improved in the sedentary group, however, the degree of the improvement in OGTT at 6 month treatment was more pronounced in the training group than in the sedentary group. 2) Amelioration of plasma insulin response during OGTT was observed after 6 months of treatment in the training group only. 3) After 2 months of treatment, glucose metabolic clearance rate(MCR) increased from 4.1㎖/㎏/min to 6.7㎖/㎏/min in the training group, but no change of MCR occurred in the sedentary group. 4) No significant changes in insulin binding to insulin receptors were observed in both the training and the sedentary groups. In conclusion, physical training improves glucose tolerance through an enhancement of insulin sensitivity and further insulin secretion in NIDDM with fasting hyperglycemia. It is useful as an adjunctive to diet in the treatment of NIDDM.

결함을 갖는 기계부품의 표면가공에 따른 압축잔류응력의 영향 및 해석

이승호,서유원,정성균 서울산업대학교 산업대학원 2000 大學院論文集 Vol.7 No.-

Shot peening was applied on both smooth and notched specimen in order to investigate the stress distribution and the improvement in fatigue strength. The results shows that the fatigue strength of shot peened specimens increases by 34 percent compared to that of unpeened ones. Compressive residual stress also increased considerably, which resulted in the increase of fatigue strength. Finite element analysis showed that shot peening is effective in decreasing the bending stress by external force. the effectiveness of shot peening in reducing the compressive residual stress was anticipated by the superposition of the concentrated stress and the compressive residual stress.

스쿼시 참가자의 참여동기와 만족도 관한 문화기술적 분석

김도호,한성유,허정석 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

This study attempts to investgate the aspects on the motives and the satsfactions of the participants of squash. This study is to analyze by ethnographic research method. The subjects in this study were 6 selected adult who were attending. The Data were collected through interviews during a period from october 2004 to december, 2004. The findings of this study were as below: First, In participating motives, casual squash participants showed participation continuous on account of health physical strength, activity motive, and accomplishment motive. Second, In participating motives, serious squash participants showed participation continuous on account of immersion motive, ostentation motive, activity motive. Third, In life satisfaction, casual squash participants felt satisfaction in physical part, psychological part. Fourth, In life satisfaction, serious squash participants felt satisfaction in physical part, social part.

제2형 당뇨병 동물모델인 OLETF 쥐에서 장기간 라미프릴 투여가 내당능 및 췌도 베타세포에 미치는 효과

고승현,윤건호,김명미,안유배,송기호,유순집,손현식,차봉연,이광우,손호영,강성구 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:최근 소개된 HOPE(Heart Outcomes Prevention Evaluation)study의 결과에 의하면, 심혈관질환이나 이에 대한 위험인자가 1가지 이상인 9,541명을 대상으로 안지오텐신 전환효소 억제제의 일종인 라미프릴을 투여하여 사망율과 뇌졸증, 심부전, 당뇨병 합병증이 개선되었을 뿐 아니라 다른 항고혈압제에 비해 새롭게 당뇨병으로 진단되는 환자가 줄었음을 보고한바 있다. 이에 저자들은 라미프릴(ramipril)을 제2형 당뇨병 동물모델에 장기간 투여함으로써 당뇨병을 예방하거나 고혈당 상태 또는 인슐린 감수성을 개선시킬수 있는지를 알아보고자 하였다. 방법:생후 24주된 체중 400∼450g의 OLETF와 LETO 쥐를 대상으로 6개월간 라미프릴 경구투여 하였고 혈압, 24시간 단백뇨 및 인슐린 내성검사를 시행하였다. 6개월 이후로는 8주간 투여군과 대조군의 체중을 비슷하게 맞추어 내당능 상태를 평가하였고 이후 다시 8주간 고농도 포도당을 투여하였다. 경구당부하검사를 시행한 후 희생시켜 조직표본을 만들어 guinea pig anti­human insulin 항체로 염색후 DAB로 발색하여 point count 법으로 베타세포를 정량하였고, 면역염색으로 췌도의 변화와 섬유화의 정도를 관찰하였다. 결과:라미프릴을 6개월간 투여하였을 때 OLETF 쥐에서 1)체중 증가가 적었으며 2)경구 당부하검사시 포도당하 면적은 의미있게 감소하였고 3)인슐린 내성검사상 Kitt가 증가되는 경향을 보였으며 4)수축기, 이완기 혈압과 24시간 단백뇨양이 의미있게 감소하였고 5)체중을 맞추면 내당능 상태가 비슷해졌다가 고농도 포도당 주입시 다시 포도당하 면적이 대조군에서 더 증가되었다. 라미프릴 투여군에서 췌도변형 및 파괴와 탈과립, 췌도내 섬유화가 감소하였다. 결론:본 실험에서는 인슐린 비의존성 당뇨병 동물 모델인 OLETF 쥐에서 당뇨병 발생 이전 단계부터 장기간 라미프릴을 투여할 경우 체중증가가 적었고, 투여군과 대조군의 체중을 맞추었을 때는 내당능상태의 차이가 없아가 고혈당 투여시 다시 투여군과 대조군에서 의미있는 차이를 보였다. 라미프릴 투여가 베타세포의 기능면에서 이로운 효과를 보일 것으로 생각되었다. 따라서 비만한 환자에서 안지오텐신 전환효소 억제제의 장기투여는 단백뇨 감소, 혈압강하 효과 이외에 체중증가의 억제 및 당뇨병 발생을 억제할 가능성을 시사한다. Background : In a Heart Outcomes Prevention Evaluation HOPE study, ramipril, a long-acting angiotensin-converting enzyme (ACE) inhibitor, significantly reduced the death rates the number of myocardial infarctions, strokes, heart failure as well as the risk of complications related to diabetes and of diabetes itself. However, it is known that ACE inhibitors improve glucose tolerance or insulin sensitivity or reduce the incidence of diabetes. Methods : 24 week-old OLETF (Otsuka Long Evans Tokushima Fatty) rats weighing 400 ~ 450 g were used in this study. 4 groups of rats were examined in parallel for 40 weeks. The OLETF rats were randomized for treatment with an aqueous solution of ramipril (5 mg/Kg) daily [OL (RMP), n=10)] and with saline [OL (CON), n=10)]. The LETO rats were also randomized in the same was as the OLETF rats (LT(RMP), n=10, LT (CON), n=10). The blood glucose level, body weight, systolic and diastolic blood pressure was assessed every month. At 3 and 6 months, the 24hrs urinary protein concentration was measured, and as insulin tolerance test and oral glucose tolerance test were conducted in all experimental groups. After 6 months, the body·weight was matched for 2 months in each corresponding group. Subsequently, a 15% sucrose loading was done for 2 months. After the glucose tolerance test, the pancreas was excised and immuno histochemical staining was conducted for insulin to quantify the beta cell mass by a point-counting method. In addition, the islet morphology was evaluated in the pancreas. Results : Ramipril treatment for a period of 6 months improved the 2hr blood glucose level, the area under the glucose curve in the oral glucose tolerance test, insulin sensitivity in addition to lowering significantly systolic and diastolic blood pressure and 24hrs urinary protein level significantly in OLETF rats. Of note, a lower weight gain was observed in both the ramipril-treated animals at 6 months. After weight matching, the AUC g and 2hr blood glucose level values were similar between the corresponding groups, but a 15% sucrose loading worsened the AUC g value. Histologically, the islets were less disorganized and the extent of fibrosis was lower in the ramipril-treated OLETF rats in the trichrome stain. Conclusion : Long-term treatment of ramipril, a long acting angiotensin-converting enzyme inhibitor may be useful for suppressing weight gain and proteinuria in addition to having a protective effect on the islet to harmful stimuli such as hyperglycemia (J Kor Diabetes Asso 25:469~482, 2001).

기어강의 표면가공에 따른 부식특성

이승호,서유원,정성균 서울산업대학교 산업대학원 2000 大學院論文集 Vol.7 No.-

The surface treatment to increasing corrosion resistance is becoming influence as very important technology. The influence of shot peening on the corrosion resistance of SCM 420 steel is investigated in this work. Then, surface compressive residual stress and hardness considerably increased, which resulted in the increase of corrosion resistance.

Cr,Cu,Fe가 첨가된 MgO 단결정의 열자극 발광 스펙트럼 분석

田耕南,兪昇喆,杜夏榮,李春鎬 全北大學校 1994 論文集 Vol.38 No.-

On the MgO single crysital doped artificially with Cr, Cu, Fe we observed thermally stimulated luminescence(TSL) glow curves and spectra, and analyzed them in the temperatured range from 500 to 77 k after excitation with UV or X-ray irradiation. TSL glow curves obtained from these samples show five peaks at 136.5K, 223.5 K, 390 K, 440 K, and their estimated activation energies are 1.08 eV, 0.27 eV, 0.63 eV, 1.19 eV, and 1.33 eV, respectively. When we measured TSL spectrum at the range of 200 nm to 650 nm, we also analysed the peak wavelength which obtained at 345 nm, 375 nm, and 410 nm from measurement of TSL spectrum and described their luminescence mechanisms.