실리카흄을 흔입한 고강도 콘크리트 개발

홍창우,김태경,김경진,김성환,김남윤,심도식,이정호,윤청호,백민경,원치문,박제선,이주형,정경일 강원대학교 석재복합신소재제품연구센터 1996 석재연 논문집 Vol.1 No.-

콘크리트 구조물이 대형화됨에 따라 설계단면이 증대되어 상대적으로 많은 경제적 손실을 부담하게 된다. 따라서 단면을 감소시키면서도 소요의 하중에 안전한 구조물을 건설하기 위해서는 우선적으로 구조물 건설에 기초가 되는 고품질, 고내구성의 고강도 콘크리트 개발이 절실히 요구된다 본 연구에서는 일정 시멘트비 및 혼화재 비율하에서 소요의 워커빌리티가 확보되는 고강도 콘크리트를 시간과 온도의 변화에 따라 증기 양생하여, 압축, 인장, 휨강도 뿐만 아니라 파괴특성을 실험적으로 연구하였다. 시료 제작시 시멘트 입자 사이의 공극 및 불연속 영역을 충전하여 고밀도화하기 위한 콘크리트용 혼화재로 시멘트 비표면적이 상당히 작은 초미립 분말인 실리카흄을 이용하였다. 또한 AE감수제 및 고성능 유동화제를 사용하여 혼화재의 첨가에 의하여 발생될 수 있는 워커빌리티의 감소를 방지하였다. 실험결과에 의하면 일정 양생 지속 시간하에서 온도의 증가에 따라 콘크리트의 압축, 인장 및 휨강도가 전반적으로 증가하였다. 동일하게 일정 온도하에서 양생 지속시간이 커짐에 따라 강도들이 증가됨을 알 수 있었다. 따라서 상대적으로 높은 온도와 긴 지속 양생 조건하에서 파괴에 대한 저항력이 크게 나타났다. The compressible, tensible, and flexibleresistance of the high strength concrete is analyzed by the experimentation in the present study. For the test, we cure several samples with the silica fume as a mixture being become dense the spaces between the particle of cement under the variation of both the temperature and the curing-interval. Then, the superplasticizer and the ezcon are also used to satisfy the required workability for construction. The compressible, tensible, and flexible resistances to a stress are increased as increasing the temperature and the time interval for the curing. Therefore it is concluded that the overal fractural and mechanical properties is improved by mixing the silica fume into the cement.

난각을 이용한 폐수중의 인 제거에 관한 연구

김민수,강선홍 대한상하수도학회 2004 상하수도학회지 Vol.18 No.2

This study is a fundamental research to test the applicability of abandoned eggshell as seed material for crystallization reaction. Eggshell was calcinated at 850℃ and ground to lesser than 0.42㎜. The calcination characteristics of eggshell were examined by X-ray diffraction (XRD). The effect of initial calcium concentration, alkalinity, reaction temperature condition, seed dosage were studied by batch test. For the low concentration sample(P concentration is under 50㎎/L), more than 90% of P can be removed. The effect of initial calcium concentration(0-120㎎/L) was performed. At the result of the test, more than 50㎎/L calcium concentration has high removal efficiency. Alkalinity effect was studied for synthetic soIution(100㎎/L initial P, 50㎎/L calcium, 0.025% seed dosage) with 0∼300㎎/L bicarbonate alkalinities. For synthetic solution(100㎎/L initial P, 50㎎/L calcium, 100㎎/L bicarbonate alkalinity, 0.025% seed dosage), the phosphorus concentration was examined with 10-35℃. In addition, calcinated eggshell was injected to swine wastewater to test the applicability to actual wastewater.

부모 기록용 아동 행동 조사표에 의한 초등학교 어린이들의 정서와 행동문제 연구

여순,홍유라,박재선,이용환,김민향 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

목적: 외견상 정상으로 간주되는 일정 지역사회 어린이들을 대상으로 문제성 정서․행동의 빈도를 알아보고, 이들 정서․행동 문제의 빈도와 사회경제적 요인들과의 관계를 알아보고자 하였다. 방법: 부산광역시의 일개 초등학교 1, 3, 5학년 어린이 700명을 대상으로 아동 행동 증상 목록표(Pediatric Symptom Checklist, PSC)로 된 설문지를 배부하였다. 설문지에는 50 항목의 행동 증상을 짧게 적고 부모가 각각의 행동 빈도에 “전혀 아님”, “가끔”및 “자주” 중 하나를 표시하도록 하였고, 아동의 건강상태, 부모 자녀간의 대화방식, 부모의 수입과 교육수준도 함께 조사하였다. 또한 일부 항목에 대해 원하는 경우, 담임선생님에 의해 이중으로 기록하게 하였다. 회수된 설문지 609매를 대상으로 빈도가 “전혀 아님”은 0, “가끔”은 1점, “자주”는 2점을 부과해 합산한 뒤 분석하였다. 결과: 피검아동 609명이 50개 문항의 행동들에서 “가끔” 또는 “자주”에 응답한 예의 빈도는 0.9-65.3%의 분포를 보였으며, 응답률에서 5%미만이 4개, 5-20%가 15개, 21-50%가 27개, 51%이상이 4개 문항이었고, 문제행동 총점들의 평균은 14.03(범위 0-43, 표준편차 8.96)점이었다. 빈도가 50%이상이었던 행동들은 “잘 울고 화내고 짜는 소리하기(총 65.2%, 남 62.75%, 여 67.9%)”, “부끄럼을 타고 남 앞에 나서기 두려움(총 60.9%, 남60.0%, 여 61.8%)”, “머리, 배, 다리가 자주 아프다(총55.8%, 남 53.1%, 여 58.8%)”, “집중하기 힘들다(총53.0% 남 55.5%, 여 50.4%)”의 4문항이었다. 성별 비교에서, 여아는 “새롭고 낯선 환경에서 겁이 많고 적응하기 힘들다”, “아무도 자신을 좋아하지 않아 희망이 없다고 느낀다”라는 두 문항에서만 남아에 비해 빈도가 높았으며(P<0.05), 문제 행동 유형별 비교시 주의산만 유 형, 공격성 유형은 남아에서, 불안은 여아에서 유의하게 높았다. 그러나 문제 행동 총점의 평균은 남녀 각각 14.44, 13.60로 유의한 차이가 없었다. 건강한 피검자 군의 총점 평균은 13.64로 이상이 있는 군의 17.29와 유의한 차이를 보였다(P=0.007). 건강한 친부모와 생활하는 피검자 군에서의 총점 평균은 13.68로 그 외 군에서의 15.92와 유의한 차이를 보였다(P=0.025). 부모자녀간의 대화방식에서 비효율적인 방법을 많이 사용하는 경우(4점이상군), 문제 행동의 총점이 16.77로서 3점 이하군의 9.48과 현저한 차이를 보였다(P<0.001). 결론: 조사 대상 어린이들의 과반수 이상은 잘 울고, 부끄러워 남앞에 나서길 두려워하며, 신체 통증을 자주 호소하고, 주의산만하다는 정서․행동의 특성을 가지고 있었다. 전체 문제 행동들의 빈도에 영향을 주는 인자로는 어린이의 외관상 건강상태, 가족 구성원의 구조, 부모의 대화습관이었고 부모의 경제력이나 학력과는 유의한 상관관계가 없었다. 문제성 정서․행동의 빈도에 가장 강력한 변인은 부모의 효율적 대화능력으로 생각되었다. Background : This study was conducted to identify the types and prevalence of emotional and behavioral problems in primary school children based on a Pediatric Symptom Checklist (PSC) which was filled out by parents. Methods : The subjects were 609 children between the age of 7 to 11 in one primary school in Busan, Korea. The PSC containing 50 behavior items were initially rated by parents and checked again by teachers in 17 items for the children whose parent consented to review. The PSC is scored by assigning 0 for never, 1 point for sometimes, and 2 point for often, and then adds the points for overall score. Parental attitudes of communication with their children were also assessed by scoring of parental responses to the questionnaire. Comparison of total behavior scores and other factors including parental competency scores were also made. Results : In each items of 50 behavioral problems, the frequency which is comprised of often true and sometimes true were widely distributed from 0.9% to 65.3%. The most common problem behavior was "crying easily and become angry"(often+sometimes: 65.2%) The next common problem behaviors were "shyness and being afraid of strange people"(60.9%), "complains of bodily aches or pains"(55.8%), "has trouble in concentrating"(53.0%), "be afraid of new situation with uneasiness"(44.5%), and "prefers to spends more time alone indoors"(43.3%) in descending order of frequency. In sexual comparison of frequency of problem behaviors, girls had significantly high frequency in two items-"be afraid of new situation with uneasiness"(P=0.018) and "no one likes him or her and has no hope" (P=0.029). On classifying 50 items into 8 behavior patterns, boys revealed higher frequencies in the behavior of inattention, impulsivity, hyperactivity and anger, aggression, antisocial activity, while girls revealed higher frequencies in the behavior of anxiety. The mean total problem scores were significantly lower in the children of healthy group (healthy 13.64 vs unhealthy 17.29, P=0.007), living with healthy biological parents (biological 13.68 vs others 15.92, P=0.025) and parents who were using less destructive communication (barrier score≤3 9.48 vs barrier score≥4 16.77, P<0.001). There was no significant correlation between the mean total problem scores and variables such as sex, grade, incomes, and educational career of mother. Conclusions : Crying easily, shyness, bodily aches, difficulty in concentrating, being afraid of new situation with uneasiness, and loneliness were commonly prevalent behaviors in the children of the primary school studied. Total problem scores were significantly higher in children with physical handicaps, children living without both biological parents, and most remarkably in children who were cared by parents with poor communication skills

Radicicol Inhibits iNOS Expression in Cytokine-Stimulated Pancreatic Beta Cells

Cha Kyung Youn,Seon Joo Park,Mei Hong Li,Min Young Lee,Kun Yeong Lee,Man Jin Cha,Ok Hyeun Kim,Ho Jin You,In Youp Chang,Sang Pil Yoon,Young Jin Jeon 대한생리학회-대한약리학회 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.4

Here, we show that radicicol, a fungal antibiotic, resulted in marked inhibition of inducible nitric oxide synthase (iNOS) transcription by the pancreatic beta cell line MIN6N8a in response to cytokine mixture (CM: TNF-Ձ, IFN-Ճ, and IL-1Ղ). Treatment of MIN6N8a cells with radicicol inhibited CM-stimulated activation of NF-ՊB/Rel, which plays a critical role in iNOS transcription, in a dose-related manner. Nitrite production in the presence of PD98059, a specific inhibitor of the extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) pathway, was dramatically diminished, suggesting that the ERK1/2 pathway is involved in CM-induced iNOS expression. In contrast, SB203580, a specific inhibitor of p38, had no effect on nitrite generation. Collectively, this series of experiments indicates that radicicol inhibits iNOS gene expression by blocking ERK1/2 signaling. Due to the critical role that NO release plays in mediating destruction of pancreatic beta cells, the inhibitory effects of radicicol on iNOS expression suggest that radicicol may represent a useful anti-diabetic activity.

노인의 Helicobacter pylori 재균율

박주상,홍원선,이영수,이은주,명승재,정훈용,양석균,김진호,민영일 대한노인병학회 2000 Annals of geriatric medicine and research Vol.4 No.3

Radicicol Inhibits iNOS Expression in Cytokine-Stimulated Pancreatic Beta Cells

Youn, Cha Kyung,Park, Seon Joo,Li, Mei Hong,Lee, Min Young,Lee, Kun Yeong,Cha, Man Jin,Kim, Ok Hyeun,You, Ho Jin,Chang, In Youp,Yoon, Sang Pil,Jeon, Young Jin The Korean Society of Pharmacology 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.4

Here, we show that radicicol, a fungal antibiotic, resulted in marked inhibition of inducible nitric oxide synthase (iNOS) transcription by the pancreatic beta cell line MIN6N8a in response to cytokine mixture (CM: TNF-${\alpha}$, IFN-${\gamma}$, and IL-$1{\beta}$). Treatment of MIN6N8a cells with radicicol inhibited CM-stimulated activation of NF-${\kappa}B$/Rel, which plays a critical role in iNOS transcription, in a dose-related manner. Nitrite production in the presence of PD98059, a specific inhibitor of the extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) pathway, was dramatically diminished, suggesting that the ERK1/2 pathway is involved in CM-induced iNOS expression. In contrast, SB203580, a specific inhibitor of p38, had no effect on nitrite generation. Collectively, this series of experiments indicates that radicicol inhibits iNOS gene expression by blocking ERK1/2 signaling. Due to the critical role that NO release plays in mediating destruction of pancreatic beta cells, the inhibitory effects of radicicol on iNOS expression suggest that radicicol may represent a useful anti-diabetic activity.

심혈관계 질환으로 관상질환 집중치료실에 입원한 환자들의 혜후 측정을 위한 APACHEⅢ점수 체계의 임상적 유용성

이공섭,이창선,최현주,홍종서,강지향,김도형,이민영,오인균,김병엽,김상억,김학찬,한승혜,이재용,지영우,안경주 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

제주도 한라산 1100 고지 대기 에어로졸의 조성 및 특성

김원형,강창희,신찬성,고선영,홍민선 濟州大學校 基礎科學硏究所 2003 基礎科學硏究 Vol.16 No.1

The atmospheric aerosols collected at the 1100 site located in Mt. Halla have been analyzed to investigate their compositions and chemical characteristics. The average concentrations of water-soluble cautions were in the order of NH_(4)^(+)>Ca^(2+)>Na^(+)>K^(+)>Mg^(2+) during the spring, showing high increase of Ca^(2+) concentration with the value of 0.60㎍/㎥, and NH_(4)^(+)>Na^(+)>K^(+)>Ca^(2+)>Mg^(2+) during the other seasons. The average concentrations of anions have shown in the order of SO_(4)^(2-)>NO_(3)^(-)>Cl^(-) for all seasons, and SO_(4)^(2-) and NO_(3)^(-) had higher concentrations in spring with the values of 4.84㎍/㎥ and 1.08㎍/㎥, respectively. From the analytical data of size-segregated particles by cascade impactor sampling, the components of NH_(4)^(+), SO_(4)^(2-), NO_(3)^(-) and K^(+) were distributed mainly in fine particles, but Ca^(2+), Na^(+), Mg^(2+) and Cl^(-) were included mostly in coarse particles. The correlation coefficients of nss-SO_(4)^(2-)/NH_(4)^(+), nss-SO_(4)^(2-)/K^(+) and NO_(3)/nss-Ca^(2+) showed quite high values with 0.871, 0.857 and 0.654, respectively. Based on the study of enrichment factors, it is considered that Na^(+), Mg^(2+), Cl^(-) and Ca^(2+) components were delivered from oceanic and soil sources, but SO_(4)^(2-), NO_(3)^(-) and NH_(4)^(+) might have other source origins. The factor analysis study showed the aerosol at the 1100 site was influenced mainly by anthropogenic factors, followed by oceanic and soil factors.

Uncoupling Protein 3의 골격근 세포내 과발현이 OLETF 백서 및 배양된 골격근 세포에서 포도당대사에 미치는 영향

한정희,박혜선,고정민,김하영,강호경,이인규,박중열,홍성관,이재담,이기업 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:Uncoupling protein(UCP)는 미토콘드리아의 내막에 위치하는 단백질로 세포내의 과다한 에너지를 열로 발산시키는 기능을 가진다. 최근 동물의 갈색지방조직에만 존재하는 UCP와 유사성을 가진 아형들(UCP2,3)이 사람에게도 존재함이 알려져 큰 관심을 끌도 있는데 이중 UCP3는 그 발현이 골격근세포와 갈색지방조직에만 국한된다. 본 연구에서는 UCP3가 체내 인슐린 감수성을 결정하는데 가장 중요한 조직인 골격근에 국한되어 발현되는 점에 착안하여 UCP3를 골격근세포에 과발현시켰을 때 포도당 대사에 어떠한 영향이 나타나는 지를 조사하였다. 방법:25주령의 8마리의 OLETF 백서를 대상으로 하여 4마리는 골격근에 adenovirus 2mL(1×10¹²pfu/mL)를 주사하여 대조군으로 하였고 4마리는 골격근에 재조합법으로 제작된 adenovirus­UCP3 2mL(1×10¹²pfu/mL)를 주사하였다(UCP3 과발현군). UCP3를 투여한 백서에서 먹이섭취가 증가하는 경향이 있어 그 전날 대조군이 먹은 야의 먹이만큼 투여하였다. 골격근에 adenovirus를 주사한 10일 후에 euglycemic hyperinsulinemic clamp를 시행하였다. Adenovirus­UCP를 C2C12 골격근 세포에 transfection시켜 UCP3를 C2C12 골격근 세포에 transfection시켜 UPS3­C2C12를 만들고 C2C12 골격근 세포와 UPS3­C2C12 골격근 세포에서 포도당 수송 및 당원합성을 측정하였다. 결과:UCP3 과발현 OLETF에서 체중이 감소하는 경향을 보였고 인슐린 감수성이 증가하였다. C2C12세포에서 기저상태 포도당 수송은 1.28±0.17μmol/L/min였고 100nM 인슐린으로 2시간 처리한 후 2.67±0.20 μmol/L/min로 증가하였다. UCP3­C2C12 세포에서는 기저상태 포도당 수송이 3.98±0.13μmol/L/min로 증가되었고 인슐린 처리 후 5.74±0.44μmol/L/min로 증가하였다. 인슐린을 처리한 UCP3­C2C12 세포에 P13K 억제제인 wortmannin을 첨가하였을 때 포도당 수송활성이 3.81±0.20μmol/L/min로 감소하였다. 기저상태 당원합성은 C2C12 세포에서 0.25±0.01μmol/L/min였고 인슐린 처리 후 0.45±0.01μmol/L/min로 증가하였다. UCP3­C2C12 세포에서는 기저상태 당원합성이 0.62±0.01μmol/L/min였고 인슐린 처리 후 1.26±454μmol/L/min로 증가하였다. UCP3­C2C12세포에 wortmannin을 첨가하였을 때 당원합성율이 0.80±0.04μmol/L/min로 감소하였다. 결론:UCP3 과발현이 OLETF 백서에서 인슐린 감수성을 증가시켰고 골격근세포에서 포도당 수송 및 당원합성을 증가시켰다. wortmannin을 첨가하였을 때 포도당 수송 및 당원합성이 감소함으로 보아 이 과정이 인슐린 신호전달체계인 P13K에 일부 의존함을 알 수 있었다. Background : UC P3 is a mitochondrial membrane protein expressed selectively in the skeletal muscle and brown adipose tissue. Since the skeletal muscle is the main organ determining insulin sensitivity in the body, it was hypothesized that UCP3 overexpression in skeletal muscle cells would improve glucose metabolism. Methods : An adenovirus-UCP3 was produced by a recombinant DNA method. OLETF rats were divided into 2 groups. Four rats were injected with the adenovirus-UCP3 (UCP3 group) and others were injected with the adenovirus(control group) in the skeletal muscle. The UCP3 group was provided with the same quantity of food as that consumed by the control group on the previous day. Insulin sensitivity was evaluated by the euglycemic hyperinsulinemic clamp method. In a separate experiment, glucose transport and glycogen synthesis we evaluated in C2C212 cells transfected with ether an adenovirus or the adenovirus-UCP3. Results : The insulin sensitivity improved significantly and the body weight decreased in the UCP3 group. The glucose transport and glycogen synthesis were higher in the UCP3-C2C12 skeletal muscle cells at the basal state. After insulin treatment, glucose transport and glycogen synthesis were also higher in the UCP3-C2C12 cells but the increments were reduced after treatment with wortmannin, a PI3K inhibitor. Conclusion : Insulin sensitivity was higher in the UCP3-overexpressed OLETF rats in the in vivo study. UCP3 transfection also increased glucose transport and glycogen synthesis in the cultured skeletal muscle cells by a PI3K dependent mechanism(J Kor Diabetes Asso 25 :460~468, 2001).

Influence of Caramel Colorant on Level of 4(5)-Methylimidazole in Coo

Min-Chul Jung,Seong Min Hong,Yu-Jin Kim,So-Jeong Yang,Min-Seon Park,Kwang-Geun Lee 한국산업식품공학회 2016 학술대회 및 심포지엄 Vol.2016 No.10

4(5)-methylimidazole (4(5)-MI) is carcinogenic, nitrogen-containing compound, mainly found during the manufacturing of caramel coloring. Hence, presence of 4(5)-MI is well-known in any food products with addition of caramel coloring for desirable sensory characteristics, such as cookies. Limited work has been conducted to develop suitable analytical method and to investigate effect of caramel coloring in cookies. This study aimed at developing the analytical method for quantification of 4(5)-MI and confirming influence of caramel coloring on level of 4(5)-Mi in cookies. Gas chromatography-mass spectrometry (GC-MS) was utilized for qualification and quantification of 4(5)-MI. Sample preparation procedure specialized in bakery products was fully developed in this study. The concentration of 4(5)-MI in 15 commercial cookies and biscuits ranged from 71.5 to 1254.8 ng/g. Correlation equation (y = 706.42x + 21.792) was obtained to estimate effect of caramel colorant on level of 4(5)-MI in cookies. Further, analytical method developed and results of correlation equation can be utilized in future studies on reduction of 4(5)-MI in many food.