The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

殺蟲性 O,O-Diethylphenylphosphate 誘導體들에 의한 Acetylcholinesterase의 Phosphorylation에 關한 量子藥理學的 硏究

明平根,成洛道,柳柄泰,李天培 충남대학교 약학대학 의약품개발연구소 1986 藥學論文集 Vol.2 No.-

Quantum pharmacological study has been carried out for the phosphorylation process of acetylcholinesterase (Ach E) and hydrolysis reaction of O,O-diethylphenylphosphate (DPP) derivatives. Quantum variables of DPp molecule were calculated by extended Hu¨ckel theory (EHT) molecular orbital calculation method and linear free energy relationship(LFER) parameters were applied to the Hamett, Taft & Swain-Luption's simple and dual substituents parameter equation. The results shows that the field effect (F) was larger than resonance effect (R) and the values of percent field (%F) and percent resonance (%R) were about %F=34 and %R=66, respectively, yielding the ratio of F to R of 2:1. From the regression analysis, the various quantum chemical parameters, the net charge of phosphorus atom and that of meta carbon atom, the LUMO energy and the electrophilic superdelocalizability were influenced significantly on the phosphorylation of Ach E. The charge-controlled and orbital-controlled reactions proceed via trigonal bypyramidal penta-coordinated intermediate with pseudorotation.

한글 문서 구조 분석에 관한 연구

우성준,김광호,유명춘,하진영 강원대학교 정보통신연구소 2008 정보통신논문지 Vol.12 No.-

Although information technology has been developed fast, large amount of machine printed document images are not properly handled due to immature document layout analysis techniques. Previous research has focused on character recognition itself to improve character recognition accuracy. Recently, however, some efforts are made to analyze complex document layout automatically. In this paper, we propose document layout analysis and understanding method for various Hangul document images. Skew detection is performed using projection profile and connected component layout information. Top-down and bottom-up methods are combined to analyze document layout. After text region and non-text region determination, table is further processed to extract row, column, and cell position. We got very high performance for skew correction, zone detection, and table processig from experiments with various Hangul document images.

소아 확장성 심근증의 임상적 고찰

이성환,김준식,박근수,김명성,권태찬,김천수,이상락 啓明大學校 醫科大學 1996 계명의대학술지 Vol.15 No.1

A Clinical assessment of the 25 patients with dilated cardiomyopathy who were admitted to the Department of Pediatrics, Keimyung University Dong San Hospital from January, 1982 to June, 1993 was performed. The result were as follows: 1) Among 25 patients with dilated cardiomyopathy. 8(32%) were younger than 1 year of age at the time of admission. 2) Adriamycin toxicity was the only encountered etiologic factor in 1 patient. 3) On admission, hepatomegaly and tachycardia were noted in almost all patients. 4) The Chest X-rays showed cardiomegalies in all 25 patients, pulmonary vein congestions in 15(60%) and pleural effusion in 2(8%). 5) The electrocardiography showed left ventricular hypertrophy in 72% and supraventricular tachycardia in 32%. 6) The echocardiographic examination showed left ventricular dilatations in all patients and left atrial dilatations in 20(80%). 7) Of 18 patients who had followed mean period of 2.9 years, 10 patients died within 9 months after the diagnosis.

INTERCALATING AGENTS의 핵산과의 상호작용에 미치는 폴리아민류의 영향에 대한 화학분광학적 연구 : 송아지 흉선 DNA-Spermine 복합체의 이례적 흡광도-온도 양상에 대한 Ethidium의 영향 Ⅰ. Ethidium Dependence of Spermine-Induced Anomalous Absorbance-Temperature Profile of Calf Thymus DNA

고동성,이천배,명평근 충남대학교 기초과학연구소 1984 연구논문집 Vol.5 No.-

Spectroscopic investigations of spermine effects on the ethidium-DNA binding equilibrium, and on the absorbance-temperature profile of the DNA in the presence of ethidium bromide at various concentrations were performed, as the ethidium binding may be used as the probe of the DNA interactions with environmental ligands. The cooperativity, enthalpy, and the midpoint of the phase transition to the trough region, of the anomalous absorbance-temperature profile, which occurs at the spermine concentration where the DNA collapses into a compact structure, are more sensitive to ethidium than the Tm region phase, and the trough can be eliminated by increased concentration of ethidium.

H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향

정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.

방사선 치료후 시간경과에 따른 염색체이상 빈도의 변화

송명재,하성환,이춘자,김희근,김미숙 대한방사선 방어학회 1994 방사선방어학회지 Vol.19 No.1

임파구의 염색체이상 빈도로부터 피폭자의 흡수선량을 구하는 방법은 사고로 인해 급성피폭을 받는 경우 유용하게 사용될 수 있다. 그러나 방사선 피폭 후 시간이 경과함에 따라 불안정 염색체이상을 가진 임파구는 감소하게 된다. 이에 방사선 치료후 시간 경과에 따른 불안정 염색체이상 빈도의 변화를 규명하고자 한다. 전골반에 50.4 Gy의 방사선 치료를 받은 총 20명의 자궁경부암 또는 자궁내막암 환자를 대상으로 41개의 검체를 얻었다. 채혈의 시기는 방사선 치료후 1일 3주, 6주, 12주, 24주, 52주, 104주, 156주, 208주, 520주로 하였다. 이들 말초혈액의 임파구에 대해 전혈미세배양을 실시한 후 임파구의 불안정 염색체이상을 관찰하여 Ydr, Qdr, Qdra를 얻었다. Ydr 값은 방사선 치료가 끝난 직후부터 3주까지 plateau를 보였고 이후 감소하는 경향이었다. Ydr의 평균값은 치료후 3주에 0.29에서 급격히 감소하여 2년후 0.05로 감소하였으며 이후 5년까지 서서히 감소하였다. 회귀분석을 실시한바 Ydr = 0.259×exp(-0.0429T)+0.0560×exp(-0.00106T)(time in weeks)로 나타났다. Qdr값은 치료 직후부터 24주까지 1.51 전후로 거의 변화가 없었으며 이후 감소하여 52주 이후에는 1.17 전후로 거의 일정하였다. Qdra 값은 치료 직후부터 12주까지 1.10 전후이며 이후 감소하여 52주 이후에는 0.81 전후였다. 피폭 후 시간경과에 따른 Ydr 값의 감소는 두 component exponential 모델을 잘 맞고 이 식을 이용하여 생물학적 선량측정이 가능하다. Qdr 값 및 Qdra 값은 피폭후 시간경과가 짧은 경우 피폭선량을 추정하는 지료로 사용할 수 있다. It is good method to use frequency of chromosome aberration in lymphocytes for a biological dosimetry in cases of accidental exposure to radiation. But in cases of past edposure, biological dosimetry is limited because the friquency of aberration decreases by time after exposure. To provide a basic data for estimationof past radiation exposure, the changing pattern of frequency of unstable chromosome aberration by time interbal after exposure was studied. Observation was made on peripheral lymphocytes of 41 blood samples from 20 patients treated for uterine cervical carcinoma and endometrial caricinoma. The patients received 50.4 Gy radiation to whole pelvis. Elapsed times after the completion of radiation therapy were 1 day, 3, 6, 9, 12, 24, 52, 104, 156, 208, 260 and 520 weeks. All the blood sample were microcultured. The Ydr, Qdr and Qdra were calculated form frequency of unstable aberration. Ydr did not decrease for 3 weeks after radiation therapy, and thereafter, decreased very rapidly and reached 0.05 at two years after radiation therapy and decreased very slowly until 5 years after radiation therapy. Relationship between unstable chromosome aberration and time interval after radiation therapy was described as Ydr = 0.2590.259×exp(-0.0429T)+0.0560×exp(-0.00106T)(time in weeks) Qdr remained constant at 1.51 until 24 weeks after radiation therapy and then decreased to 1.17 at 52 weeks. Therafter, it did not change. Qdra remained constant at 1.10 for 12 weeks after radiation therapy and decreased to 0.81 at 52 weeks. Thereafter, it remanined constant. Two superimposed exponential Ydr disappearance rate wuggests that it is possible to calculate the past exposure dose. When the elapsed time after exposure is short, Qdr and Qdra are useful papameters for biological dosimetry for past radiation exposure.

변류기 포화가 거리계전 알고리즘에 미치는 영향 분석

孫天明,姜相熙 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

Abstract - Distance relays need accurate current and voltage for determining the trip ignition. Therefore we must analyze nonlinear phenomena which cause distortion in signals first of all. This paper presents the effect of current transformer saturation in power system. The saturation of a current transformer distorts the input current of a distance relay. This paper describes modeling methods of a current transformer, simulation results of two distance relay algorithms (discrete fourier transform and modified differential equation methods) and some interesting results of the algorithm by using an CT secondary current compensating technique.

일반주거환경이 기관지과민성에 미치는 영향

김상섭,전형준,백도명,김록호,김호 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : The increased prevalence of asthma over the last 30 years has been reported from many different countries, including Korea. The increased prevalence may be due to increased exposure to indoor air pollutants. Methods : In this study, the relationship between residential environments and airway hyper-responsiveness has been examined among 280 family members. The bronchial hyper-responsiveness was expressed as the log-transformed slope of FEV1 decrease over the log-transformed dose of methacholine concentration (BRindex). Simple regression analysis was done for the effect of sex, age, atopic status, and residential environments including active and passive smoking exposures on airway responsiveness. Based on simple regression results, multiple rwegression analysis was performed for total group and also according to sex. Results : The mean of BRindex of residents living in gas boiler heating houses was higher than that of central heating system (p=0.014). Smoking status was a significant determinant of bronchial hyper-responsiveness in both genders, male (p=0.017), female (p=0.022). In the male group the mean of BRindex of current smokers was significantly higher than those of ex-smokers and non-smokers, and the mean of BRindex of passive smokers was higher than that of non-smokers, but statistical significance was border-line(p=0.069). In female group, the mean of BRindex of kerosene stove users was higher than that of non-users (p=0.057). Conclusions : This study suggests that indoor air pollutants including type of heating, passive smoking and kerosene stove use can contribute to the increase in asthma prevalence in Korea.

p-Nitrostyrylphenylsulfone 基質에 對한 L-Cysteine의 反應

李天培,成洛道,明平根 충남대학교 자연과학연구소 1980 學術硏究誌 Vol.7 No.1

The kinetics and mechanism of nucleophilic addition of L-Cysteine to P-nitrostyrlphenyl-sulfone substrate over wide pH range was presented and analyzed in detail. The rate constants were determined by ultra-violet spectrophotometry in 50% methanol water mixed solvent at 25℃ and the following rate equation which can be applied over wide pH range was obtained. i.e kt = ko + kp [OH^(-)] =(1.92×10^(-3)+3.71×10^(3)[OH^(-)]/8.94×10^(-2)+1.03×10^(5)[OH^(-)]) +4.56×10^(2)OH^(-)] From the rate equation, the following mechanism were proposed; above pH 10. 50, the reaction proceed by the attack of L-Cy-S^(-) on the activated carbon-carbon double bind of substrate. Below pH 8.50, the reaction proceed by addition of L-Cysteine to p-nitrostyrl phenylsulfone substrate. And in the range of pH 8.50-10.50, these two reaction occur competitively.