http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Molecular Changes in Remote Tissues Induced by Electro-Acupuncture Stimulation at Acupoint ST36
Sam-Woong Rho,Gi-Soon Choi,Eun-Jung Ko,김선광,Young-Seop Lee,이혜정,홍무창,신민규,민병일,Hyun-Jung Kee,이철구,배현수 한국분자세포생물학회 2008 Molecules and cells Vol.25 No.2
To investigate the effects of electro-acupuncture (EA) treatment on regions remote from the application, we measured cellular, enzymatic, and transcriptional activities in various internal tissues of healthy rats. The EA was applied to the well-identified acupoint ST36 of the leg. After application, we measured the activity of natural killer cells in the spleen, gene expression in the hypothalamus, and the activities of antioxidative enzymes in the hypothalamus, liver and red blood cells. The EA treatment increased natural killer cell activity in the spleen by approximately 44%. It also induced genes related to pain, including 5-Hydroxytryptamine (serotonin) receptor 3a (Htr3a) and Endothelin receptor type B (Ednrb) in the hypothalamus, and increased the activity of superoxide dismutase in the hypothalamus, liver, and red blood cells. These findings indicate that EA mediates its effects through changes in cellular activity, gene expression, and enzymatic activity in multiple remote tissues. The sum of these alterations may explain the beneficial effects of EA
Rho, Sam-Woong,Chung, Hwan-Suck,Kang, Moon-Kyu,Na, Young-In,Cho, Chong-Woon,Kim, Hyung-Min,Jung, Hyuk-Sang,Park, Hi-Joon,Kim, Hong-Yeoul,Hong, Moo-Chang,Shin, Min-Kyu,Kim, Sung-Soo,Bae, Hyun-Su The Korean Society of Toxicogenomics and Toxicopro 2005 Molecular & cellular toxicology Vol.1 No.4
Cirsii Japonici Herba (CJH) extract has been used for hundreds of years in Asian countries as a treatment for pollutant, radiation, and alcohol-induced liver damage. The reducing effect of CJH on hydrogen peroxide-induced reactive oxygen species (ROS) production, the main cause of cell damage or death, was evaluated using the HepG2 cell line. Cell survival was determined using MTS assay. The viability of cells treated with CJH was not significantly different from oxidative-stressed HepG2 cells. A dose-dependent inhibitory effect by CJH on ROS production was shown in oxidative-stressed cells using the $H_{2}DCFDA$ assay. To identify candidate genes responsible for the anti-oxidative effects of CJH on HepG2 cells, an oligonucleotide microarray analysis was performed. The expressions of five genes were decreased, whereas nineteen genes were up-regulated in CJH plus hydrogen peroxide treated cells, compared to only hydrogen peroxide treated cells. Among them, the expression of 5 genes was decreased in hydrogen peroxide treatment when compared to control. These genes are known to regulate cell survival and progression. On the other hand, it was shown that its main compounds were not a sylimarin or its analogs. The list of differentially expressed genes may provide further insight on the action and mechanism behind the anti-oxidative effects of Cirsii Japonici Herba.
cDNA microarray를 이용한 침자극에 대한 반응군과 비반응군의 유전자적 특이성 연구
노삼웅,이기석,이진우,홍무창,신민규,민병일,배현수 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.4
Numerous studies show that acupuncture stimulation increases experimental pain threshold in various animal species. It has been suggested that the acupuncture analgesic effects could be mediated by endogenous opioid system. However, the analgesic effect on the rat in response to electroacupuncture(EA) shows marked individual variations. Individual variations in effectiveness(IVE) of acupuncture analgesia has frequently provoked clinical troubles. The intrinsic mechanisms that cause IVE are an interesting subject for the study. The purpose of this study is to identify and characterize genes that cause differences between the responder and non-responder upon EA treatments using cDNA microarray. Sprague-Dawley male rats were stimulated at the zusanli(ST36) acupoint, then the tail flick latency (TFL) test was performed to classify the responder and non-responder group. Among the 19 rats tested, the ratio of responder group to non-responder group was 6:4. The hypothalamus of each group was dissected and mRNA was further purified. After synthesizing cDNA using oligo-dT primer, the cDNA were applied to Incyte rat GEMTM 2 microarray chips. The microarray results show that genes such as Brain factor(BF)-1, a transcription factor essential for the proliferation of the progenitor cell of the cerebral cortex, Dopamine beta-hydroxylase and Phosphotidylethanolamine N-methyltransferase which play a major role in catecholamine synthesis, are expressed abundantly in the responder group, whereas tau and cathepsin B, which are neurodegeration markers are abundant in the non-responder group. These results suggest that the sensitivity of EA mediated analgesic effects are closely related with neurodegeneration of the brain.
Soo-Jin Kim,Byung-Sam Kim,Tae-Wook Kyung,이상철,Chi-Woong Rho,Kyung-Rak Choi,Hae-Jun Hwang,Hye-Seon Choi 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.3
The oral administration of extracts of young radishes cultivated with sulfur after intravenous tumor cell injection achieved a marked reduction of pulmonary colonization in mice. Treatment of the mice with extracts of young radish cultivated with sulfur did not show any increase in the number of CD8+ or NK T cells in the spleen, indicating no influence on host immunity. Sulforaphane, which could be a candidate for an active compound from young radishes cultivated with sulfur, inhibited cell growth of B16-F10 melanoma cells. In addition, extracts of the young radish cultivated with sulfur-fed group showed enhanced quinine reductase (QR) activities in the liver and lung and a slight increase of glutathione S-transferase (GST) activity in the liver. These results suggested that the administration of extracts of young radishes cultivated with sulfur suppressed pulmonary tumorigenesis, possibly due to increased activity of detoxification enzymes in the liver and lung, and partly due to cell cytotoxicity.
牡丹皮의 PC12 cell 항산화 효과와 관련 HO, MIF, COMT 유전자 발현에 미치는 영향
손무성,노삼웅,고은정,나영인,조종운,배현수,홍무창,신민규 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.4
Oriental medicine explains aging as the weakening of Kidney-Qi, and Kidney-strengthening herbal medicines such as Yukmijihwang-tang have been studied for anti-aging effects. In Western Medicine, the hypothesis that reactive oxidant species(ROS) contribute to the aging process is generally accepted. It has been reported that Moutan Cortex Radicis extract (MCR) was the most effective constituent of Yukmijihwang-tang in decreasing ROS production in oxidative-stressed cells. The purpose of this study is to confirm the anti-oxidant effect of MCR on PC12 cells, the expression of Heme oxygenase (HO), Macrophage migration inhibitory factor (MIF), Catechol-0-methyltransferase (COMT) using real time RT PCR. PC12 cells were treated without or with hydrogen peroxide in the presence or absence of MCR using MTS assay. Hydrogen peroxide decreased the viability of PC12 cells by 53% and MCR did not influence that of stressed PC 12 cells irrespective of dose or incubation period. However, MCR showed an inhibitory effect on production of ROS in stressed cells, both dose and incubation time dependently. In particular, 1 mg/ml of MCR for 24 h culture almost returned to normal level. In the quantiation of anti-aging related gene expression, MCR at 1 mg/ml increased the expression of HO by 370%, MlF by 180% and COMT by 280% through real time RT PCR. In conclusion, MCR treatment protected PC12 cells from hydrogen peroxide and decreased ROS production and enhanced anti-oxidative gene expression such as HO, COMT and MlF, which suggests that MCR is involved in controlling anti-aging of nerve cells through elimination of cytotoxic stimuli..
牡丹皮의 PC12 cell 酸化抑制 효과 및 neuronal 유전자 발현 profile 분석에 대한 연구
김현희,노삼웅,나영인,배현수,신민규,김정숙,홍무창 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.2
Yukmijihwang-tang has been widely used as an anti-aging herbal medicine for hundred years in Asian countries. Numerous studies show that Yukmijihwangtang has anti-oxidative effect both in vivo and in vitro. It has been reported that Moutan Cortex Radicis extract (MCR) was the most effective herb in Yukmijihwang-tang on undifferentiated PC12 cells upon oxidative-stressed with hydrogen peroxide. The purpose of this study is to; 1) evaluate the recovery of neuronal damage by assessing the anti-oxidant effect of MCR on PC12 cells differentiated with nerve growth factor (NGF), 2) identify candidate genes responsible for anti-oxidative effect on differentiated PC12 cells by oligonucleotide chip microarray. PC12 cells, which were differentiated by treating with NGF, were treated without or with hydrogen peroxide in the presence or absence of various concentration of MCR. Cell survival was determined by using MTS assay. Measurement of intracellular reactive oxygen species (ROS) generation was determined using the H2DCFDA assay The Viability of cells treated with MCR was significantly recovered from stressed PC12 cell. In addition, wide rage of concentrations of MCR shows dose-dependent inhibitory effect on ROS production in oxidative-stressed cells. Total RNAs of cells without treatment(Control group), only treated with H_2O_2 (stressed group) and treated with both H2O2 and of MCR (MCR group) were isolated, and cDNAs was synthesized using oligoT7(dT) primer. The fragmented cRNAs, synthesized from cDNAs, were applied to Affymetrix GeneChip Rat Neurobiology U34 Array. mRNA of Calcium/calmodulin-dependent protein kinase Ⅱ delta subunit(CaMKⅡ), neuron glucose transporter (GLUT3) and myelin/oligodendrocyte glycoprotein(MOG) were downregulated in Stressed group comparing to Control group. P2X2-5 receptor (P2X2R-5), P2X2-4 receptor (P2X2R-4), c-fos, 25 kDa synaptosomal attachment protein(SNAP-25a) and GLUT3 were downregulated, whereas A2 adenosine receptor (A2AR), cathechol-O-methyltransferase(COMT), glucose transporter 1 (GLUT1), EST223333, heme oxygenase (HO), VGF, UI-R-C0-ja-a-07-0-UI.s1 and macrophage migration inhibitory factor (MIF) were upregulated in MCR group comparing to Control group. Expression of Putative potassium channel subunit protein (RCK4), P2X2R-5, P2X2R-4, Interferon-gamma inducing factor isoform alpha precursor (IL-18α), EST199031, P2XR, P2X2 purinoceptor isoform e (P2X2R-e), Precursor interleukin 18 (IL-18) were downregulated, whereas MOG, EST223333, GLUT-1, MIF, Neuronatin alpha, UI-R-C0-ja-a-07-0-UI.s1, A2 adenosine receptor, COMT, neuron-specific enolase (NSE), HO, VGF, A rat novel protein which is expressed with nerve injury (El2625) were upregulated in MCR group comparing to Stressed group. The results suggest that decreased viability and ROS production of PC12 cell by H_2O_2 may be, at lease, mediated by impaired glucose transporter expression. It is implicated that the MCR treatment protect PC12 cell from oxidative stress via following mechanisms; improving glucose transport into the cell, enhancing expression of anti-oxidative genes and protecting from dopamine cytotoxicity by increment of COMT and MIF expression. The list of differentially expressed genes may implicate further insight on the action and mechanism behind the anti-oxidative effects of herbal extract Moutan Cortex Radicis.
육미지황탕가미방에 의한 흰쥐 기억력 향상과 관련된 Hippocampus 부위의 특이 유전자 발현에 대한 연구
심대식,노삼웅,이진우,이은아,조종운,배현수,신민규,홍무창 대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.3
The herbal extract(YMT_02) is a modified extracts from Yukmijihwang-tang(YMJ) to promote memory-enhancing. The YMJ extracts has been widely used as replenishing yin and tonifying the kidneys herbal medicine for hundred years ia Asian countries. The purpose of this study is to: 1) quantitatively evaluate the memory-enhancing effect of YMT_02 by passive avoidance test, 2) statistical evaluation of candidate gene expression (pentraxin, PEP-19, transthyretin) in rat hippocampus. The hippocampi of YMT_02 and control group were dissected and mRNA was further purified. After synthesizing cDNA using oligo-dT primer, the cDNA were applied to Real Time PCR. The results were as follows : 1) passive avoidance test showed enhancing memory retentin by YMT_02 treatment, 2) expression of pentraxin, that accelerate degenerating of neuronal cell, was significantly decreased, 3) the mRNA of genes that has been known to be associated with protecting neuronal cell degeneration, such as PEP-19 and transthyretin, were significantly increased upon YMT_02 treatment. From above results, the administration of YMT_02 which tonify the function of Kidneys could enhance the ability of memory and learning. In addition, the administration of YMT_02 enhance memory retention through modulating particular gene (pentraxin, PEP-19, transthyretin) expressions in hippocampu