국내 분리 일본뇌염 바이러스 유전자에 대한 항체생성 연구(Ⅰ) : cloning of JEV viral genes

박찬,최우영,이호동,채수림,신은진,김인범,표재환,박근용,조해월 국립보건연구원 1999 국립보건원보 Vol.36 No.-

한국 성인의 정상 음성에 관한 기본 음성 측정치 연구

표화영,심현섭,송윤경,윤영선,이은경,임성은,하현령,최홍식 한국음성과학회 2002 음성과학 Vol.9 No.2

Our present study was performed to investigate acoustically the Korean normal adults'; voices, with enough large number of subjects to be reliable. 120 Korean normal adults (60 males and 60 females) of the age of 20 to 39 years produced sustained three vowels, /a/, /i/, and /u/ and read a part of 'Taking a Walk' paragraph, and by analyzing them acoustically with MDVP of CSL, we could get the fundamental frequency (F_0), jitter, shimmer and NHR of sustained vowels; speaking fundamental frequency (SF_0), highest speaking frequency (SFhi), lowest speaking frequency (SFIo) of continuous speech. As results, on the average, male voices showed 118.1~122.6 ㎐ in F_0, 0.467~0.659% in jitter, 1.538~2.674% in shimmer, 0.117~0.133 in NHR, 120.8 ㎐ in SF_0, 183.2 ㎐ in SFhi, 82.6 ㎐ in SFIo. And, female voices showed 211.6~220.3 ㎐ in F0, 0.678~0.935% in jitter, 1.478~2.582% in shimmer, 0.098~0.114 in NHR, 217.1 ㎐ in SF_0, 340.9 ㎐ in SFhi, 136.0 ㎐ in SFIo. Among the 7 parameters, every parameters except shimmer showed the significant difference between male and female voices. And, when we compared the three vowels, they showed significant differences one another in shimmer and NHR of both genders, but not in F_0 of males and jitter of females.

Micromechanics-based elastic-damage analysis of laminated composite structures

Pyo, S.H.,Lee, H.K. Pergamon Press ; Elsevier Science Ltd 2009 International journal of solids and structures Vol.46 No.17

Based on the micromechanics-based constitutive model, derived in our preceding work [Lee, H.K., Pyo, S.H., 2009. A 3D-damage model for fiber-reinforced brittle composites with microcracks and imperfect interfaces. Journal of Engineering Mechanics-ASCE, in press, doi:10.1061/(ASCE)EM.1943.7889.0000039.], incorporating a multi-level damage model and a continuum damage model, the overall elastic behavior and damage evolution of laminated composite structures are studied in detail. The constitutive model is implemented into the finite element program ABAQUS using a user-subroutine UMAT to solve boundary value problems of the composite structures. The validity of the implemented constitutive model is assured by comparing the predicted stress-strain curves with experimental data available in literature under uniaxial tension with various fiber orientations. The results show that the proposed micromechanics-based constitutive model accurately predict the overall elastic-damage behavior of laminated composite structures having different material compositions, thicknesses and boundary conditions.

적외선 우주배경복사 관측 실험 2(CIBER2) 국제 공동 연구

이대희,박원기,문봉곤,박성준,김민규,김건희,남욱원,표정현,정웅섭,박영식,김일중,한원용,Lee, D.H.,Park, W.K.,Moon, B.G.,Park, S.J.,Kim, M.G.,Kim, G.H.,Nam, U.W.,Pyo, J.,Jeong, W.S.,Park, Y.S.,Kim, I.J.,Han, W. 한국천문학회 2015 天文學論叢 Vol.30 No.1

First light galaxies have predictable linear clustering, and are expected to produce fluctuations with a characteristic spatial power spectrum, which peaks at an angular scale of ~ 10 arcminutes and in the $1-2{\mu}m$ spectral regions. The Cosmic Infrared Background ExpeRiment 2 (CIBER2) is a dedicated sounding rocket mission for measuring the fluctuations in the extragalactic infrared background light, following up the previous successful measurements of CIBER1. With a 28.5 cm telescope accompanied with three arms of camera barrels and a dual broadband filter on each H2RG (${\lambda}_c=2.5{\mu}m$) array, CIBER2 can measure 6 bands of wide field ($1.1{\times}2.2$ degrees) up to 3 AB magnitudes deeper than CIBER1. This project is leaded by California Institute of Technology/Jet Propulsion Laboratory, collaborating internationally with Institute of Space and Astronautical Science in Japan, Korea Astronomy and Space Science Institute, Korea Basic Science Institute, and Seoul National University. The Korean team is in charge of 1) one H2RG scientific array, 2) ground station hardware and software, 3) telescope lenses, and 4) flight and test bed electronics fabrication. In this paper, we describe the detailed activities of the Korean participation as well as the current status of the CIBER2 project.

糖尿病을 合倂한 上腹部臟器의 部分傳位症

朴俊明,李公錫,表潤浩,李鍾星 中央醫學社 1940 中央醫學 Vol.9 No.1

당뇨병에서의 혈장 Renin 활성에 관한 연구

이정상,이문호,김성권,최강원,표희정,박정식 대한핵의학회 1979 핵의학 분자영상 Vol.13 No.1

당뇨병에서의 고혈압 및 신합병증에 따른 Renin Angiotensin-Aldosterone계의 동태를 밝히기 위하여 40예의 당뇨병 환자에서 기저 및 furosemide 정주 2시간 후의 PRA를 측정하였다. 1) 40예의 당뇨병 환자는 신합병증과 고혈압의 유무에 따라 4군으로 분류하였으며, 임상적인 합병증이 없는 Ⅰ군에는 15명, 신합병증이 있는 Ⅱ군에는 7명, 신합병증과 고혈압이 같이 있는 Ⅲ군에는 8명, 고혈압만 있는 Ⅳ군에는 10명의 환자가 속하였다. 2) 각 군의 24시간 요중 Na배설량의 평균치 사이에는 유의한 차이가 없었다. 3) 기저 PRA치는 Ⅰ군이 1.53±1.09ng/ml/hr(Mean±S.D), Ⅱ군이 0.63±0.59ng/ml/hr, Ⅲ군이 0.79±0.62ng/ml/hr, Ⅳ군이 1.11±0.80ng/ml/hr로서 Ⅱ군과 Ⅲ군은 Ⅰ군에 비하여 유의하게 저하되어 있으나(P$lt;0.05), 다른 군들 간에는 유의한 차를 볼 수 없었다. 4) furosemide 정주 2시간 후의 PRA치는 Ⅰ군이 2.72±1.96ng/ml/hr, Ⅱ군이 0.92±0.78ng/ml/hr, Ⅲ군은 1.03±0.76ng/ml/hr, Ⅳ군은 1.73±1.39ng/ml/hr로써 Ⅰ군과 Ⅱ군사이에서만 유의한 차이를 보이고(P$lt;0.05), 다른 당뇨병 환자군 사이에서는 유의한 차를 볼 수 없었다. 5) furosemide 정주에 의한 Renin 분필 자극시험에 대한 반응도 Ⅱ군과 Ⅲ군에서는 둔화되어 있는 양상을 보이나, 시험대상도 적고하여 통계적 유의성에는 미치지 못하였다. 이상의 성적에서 신합병증이 있는 당뇨병에서는 혈장 Renin활성이 저하되며 furosemide 정주에 의한 Renin 분필자극시험에 대한 반응도 둔화됨을 알 수 있었다. 또한 당뇨병성 신합병증에 의한 고혈압에서도 혈장 Renin활성은 저하되는 경향을 보여서 이 경우의 고혈압은 Renin이외의 다른 요인이 작용할 것으로 보인다. To evaluate the renin-angiotensin-aldosterone system in diabetes mellitus, basal plasma renin activity(PRA) and its response to intravenous furosemide were determined in 40 diabetic subjects. The diabetics were divided into 4 groups according to the pressence of nephropathy and/or hypertension. Uncomplicated diabetics(Group I) were taken as control group and the results of the other groups were compared to this group. In diabetics viith nephropathy alone(Group Ⅱ), and with nephropathy and hypertension (Group Ⅲ), basal PRA values were 0.63±0.59ng/ml/hr., and 0.79±0.62ng/ml/hr., respectively, both significantly lower than control group. (1.53±1.09ng/ml/hr.). (P$lt;0.05) In both of the above groups, the responses to intravenous furosemide tended to be blunted. On the other hand, in diabetics, with hypertension only (Group Ⅳ), the basal and stimulated PRA were not significantly different from control. Above results suggests that nephropathy may be one of the factors which suppress renin activity in diabetes me.

만성신부전증에서 혈청 CEA치에 관한 연구

이정상,신영태,정순일,고창순,김성권,표희정,권인순 대한핵의학회 1980 핵의학 분자영상 Vol.14 No.2

저자들은 1979년 3월부터 1980년 10월까지 서울대학교병원내과에 입원하여 만성신부전증으로 진단받고 혈액투석요법을 받지 않은 환자 15예, 혈액투석요법을 시행중인 환자 39예 및 신장이식술을 받고 신장능이 호전된 환자 23예에서 혈청 CEA치를 방사면역측정법으로 측정하여 정상대조군과 비교하여 다음과 같은 결과를 얻었다. 1) 정상 성인 65명의 혈청 CEA치의 범위는 1.0∼4.3 ng/ml이었으며 평균은 1.6±0.66 ng이었다. 2) 혈액투석요법을 시행치 않은 만성신부전증 환자 15예의 혈청 CEA치는 0.3∼8.3 ng/ml이었고 평균은 3.6±2.10 ng/ml로써 정상대조군에 비하여 유의하게 증가되어 있었다(p$lt;0.001). 3) 혈액투석요법으로 치료중인 만성신부전증 환자 39예의 혈청 CEA치는 0.7∼6.7 ng/ml로 평균이 3.0±1.52 ng/ml이며, 정상대조군에 비하여 유의하게 상승되어 있으나(p$lt;0.001), 혈액투석을 받지 않은 환자군과는 유의한 차이가 없었다. 4) 신이식 환자 23예의 혈청 CEA치의 분포는 1.8∼10.8 ng/ml이고 평균은 3.8±1.96 ng/ml로 역시 정상대조군에 비하여 유의하게 증가되어 있으나(p$lt;0.001), 만성신부전증 환자군과는 유의한 차이가 없었다. 5) 이상의 혈청 CEA치가 정상범위 이상으로 상승된 49명��환자중 이학적 소견이나 검사소견상 악성종양은 발견되지 않았다. The serum CEA levels were measured by radioimmunoassay technique in 15 patients with chronic renal failure, who were not treated with hemodialysis, in 39 patients under hemodialysis and in 23 patients who received renal transplantation. The results were compared with those in 65 normal adults and the following results were obtained. 1) Serum CEA concentrations in 65 normal adults were in the range of 1.0 to 4.3 ng/ml with a mean value of 1.6±0.66 ng/ml. 2) Serum CEA concentrations in 15 chronic renal failure patients who were not treated with hemodialysis, were in the range of 0.3 to 8.3 ng/ml with a mean value of 3.6±2.10 ng/m1 which was significantly higher than those of normal controls(P$lt;0.001). 3) Serum CEA concentrations in 39 chronic renal failure patients under hemodialysis were also much higher than normal controls(p$lt;0.001), but not significantly different from those of the patients who were not under hemodialysis(P$gt;0.05). 4) In 23 patients who received renal transplantation, serum CEA levels were snificantly higher than normal controls(P$lt;0.001), but not significantly different from those of chronic renal failure patients.

Effect of citric acid on the acidification of artificial pepsin solution for metacercariae isolation from fish

Kim, M.K.,Pyo, K.H.,Hwang, Y.S.,Chun, H.S.,Park, K.H.,Ko, S.H.,Chai, J.Y.,Shin, E.H. Elsevier Scientific Pub. Co 2013 Veterinary parasitology Vol.198 No.1

Artificial digestive solution based on pepsin is essential for collecting metacercariae from fish. To promote the enzymatic reactivity of pepsin, the pH of the solution has to be adjusted to pH 1.0-2.0. Hydrochloride (HCl) is usually used for this purpose, but the use of HCl raises safety concerns. The aim of this work was to address the usefulness of citric acid as an alternative for HCl for the acidification of pepsin solution, and to examine its potential to damage metacercariae during in vitro digestion as compared with HCl. Changes in pH after adding 1-9% of citric acid (m/v) to pepsin solution were compared to a 1% HCl (v/v) addition. Digestion of fish muscle was evaluated by measuring released protein concentrations by spectrophotometry. In addition, survival rates of metacercariae in pepsin solution were determined at different citric acid concentrations and were compared that of with 1% HCl. The present study shows that addition of citric acid reduced the pH of pepsin solutions to the required level. Addition of more than 5% of citric acid resulted in the effective digestion of fish muscle over 3h in vitro, and 5% citric acid was less lethal to metacercariae than 1% HCl in pepsin solution. Pepsin solution containing 5% citric acid had digestive capacity superior to pepsin solution containing 1% HCl after 3h incubation with released protein concentrations of 12.0ng/ml for 5% citric acid and 9.6ng/ml for 1% HCl. Accordingly, the present study suggests that the addition of 5% citric acid to pepsin solution is a good alternative to 1% HCl in infection studies because citric acid is a stable at room temperature and has a good safety profile. In addition, we suggest that the use of citric acid enables the preparation of commercial digestive solutions for the detection of microorganisms in fish and other vertebrate muscle tissue.

Transparent and mechanically robust flexible heater based on compositing of Ag nanowires and conductive polymer

Pyo, K.h.,Kim, J.W. Elsevier Applied Science Publishers 2016 Composites science and technology Vol.133 No.-

A highly flexible and transparent heater was developed from a composite electrode comprising silver nanowires (AgNWs) and a conductive polymer (CP) embedded in a colorless polyimide (cPI) film by a solution-processed inverted layer-by-layer processing method. The resulting CP-AgNW/cPI heater was mechanically stable with increases in resistance of <10% under long-term cyclic bending to a curvature radius of 500 μm for up to 50,000 cycles. A roughness measurement revealed that the high flexibility originated from the electrodes being fully embedded at the surface of the polymer. The fabricated electrode was rapidly heated from room temperature to steady-state temperature within 40 s, with a peak temperature exceeding 200 <SUP>o</SUP>C. AgNW hybridization with CP layers was more efficient at lower NW densities, demonstrated by comparisons between the measured and calculated sheet resistance (R<SUB>s</SUB>) of the electrodes. The addition of CP to the existing AgNW electrodes enhanced the steady-state temperature and current-carrying capacity of the fabricated heaters. The results obtained in this study demonstrated that the proposed method of inverted layer-by-layer processing of AgNWs and CP is suitable to achieve a highly reliable and efficient transparent heater.

Neuroprotective Effect of trans-Cinnamaldehyde on the 6-Hydroxydopamine-Induced Dopaminergic Injury

Pyo, J.-H.,Jeong, Y.-K.,Yeo, S.,Lee, J.-H.,Jeong, M.-Y.,Kim, S.-H.,Choi, Y.-G.,Lim, S. PHARMACEUTICAL SOCIETY OF JAPAN 2013 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.36 No.12