아세틸 콜린 유발 검사시 허혈성 심전도 변화와 흉통의 발생이 내피세포 기능장애에 미치는 영향

최철웅(Cheol Ung Choi),나승운(Seung-Woon Rha),김선원(Sun Won Kim),나진오(Jin Oh Na),임홍의(Hong Euy Lim),김진원(Jin Won Kim),김응주(Eung Ju Kim),한성우(Seong Woo Han),박창규(Chang Gyu Park),서홍석(Hong Seog Seo),오동주(Dong Joo Oh) 대한임상노인의학회 2010 대한임상노인의학회지 Vol.11 No.4

연구배경: 아세틸 콜린 유발 검사시 발생하는 흉통과 허혈성 심전도 변화가 아세틸 콜린에 의해 발생하는 관상동맥의 내피세포 기능에 어떤 영향을 주는지 알아보고자 했다. 방법: 흉통으로 내원하여 관상동맥 조영술과 아세틸 콜린 유발검사를 시행받은 1,085명의 환자를 대상으로 하였다. 아세틸 콜린 유발 검사를 하는 동안 허혈성 심전도 변화 및 흉통의 발생 유무에 따른 관상동맥 내피세포 기능의 특징을 비교하였다. 결과: 539명의 환자에서 아세틸 콜린 검사시 내피세포 기능 장애가 발생 하였다. 허혈성 심전도 변화가 있었던 그룹이 심전도 변화가 없었던 그룹보다 흉통이 더 많았고(78.1% vs. 60.8%, P=0.007), 관상동맥 조영술상 다발성 관상동맥 경련이 빈번하였고(59.4% vs. 40.6%, P=0.004), 관상동맥의 경련 정도도 더 심하였다(64.1% vs. 46.5%, P=0.006). 흉통이 있었던 그룹이 흉통이 없었던 그룹보다 허혈성 심전도 변화의 빈도가 더 높았다(14.7% vs. 7.5%, P=0.007). 또한 흉통이 있었던 그룹이 관상동맥 경련이 다발성으로 발생하는 경우가 많았고 (50.7% vs. 29.5%, P<0.001), 미만성으로 발생하는 경우가 많았고(87.1% vs. 75%, P<0.001), 관상동맥의 경련의 정도도 더 심하였다(51.6% vs. 43.5%, P=0.041). 결론: 아세틸 콜린 유발검사시 발생하는 허혈성 심전도 변화와 흉통은 관상동맥 경련의 정도와 경련이 발생하는 관상동맥 개수와 연관이 있다. Background: ECG changes and chest pain during acetylcholine (Ach) provocation tests may constitute important clinical parameters for Ach-induced endothelial dysfunction. We investigated the association between ischemic ECG changes and chest pain during the Ach-provocation test and angiographic characteristics of Ach-induced endothelial dysfunction. Methods: A total of 1,085 patients with anginal symptoms underwent diagnostic coronary angiography (CAG) and Ach-provocation tests. We compared angiographic characteristics of Ach-induced endothelial dysfunction according to the presence of ECG change and chest pain. Results: A total of 539 patients experienced Ach-induced endothelial dysfunction. Patients who experienced ECG change group suffered more frequent chest pain (78.1% vs. 60.8%, P=0.007), angiographically more frequent multiple coronary artery spasm (59.4% vs. 40.6%, P=0.004), and more severe coronary artery spasm (64.1% vs. 46.5%, P=0.006) than patients without ECG change. However, there was no difference in the length of endothelial dysfunction between the groups. The incidence of ischemic ECG changes in patients with chest pain was higher than in patients without chest pain (14.7% vs. 7.5%, P=0.007). Patients who experienced chest pain more often experienced multiple (50.7% vs. 29.5%, P<0.001), diffuse (87.1% vs. 75%, P<0.001), and severe coronary artery spasm (51.6% vs. 43.5%, P=0.041) than patients without chest pain. Conclusion: Ischemic ECG changes and chest pain during the Ach-provocation test are associated with multi-vessel involvement and severe coronary artery spasm. Chest pain is associated with the length of endothelial dysfunction, but ischemic ECG change is not.

Quality change during preservation of cultivation mushrooms, Flammulina velutipes and Agaricus bisporus

Jin-A Oh,Jong-Chun Cheong,Chan-Jung Lee,Chang-Sung Jhune,Young-Bok Yoo 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

The standard does much to improve merchantable quality, distribution efficiency and fair dealings by shipping of the standard agricultural products. Mushrooms notified as the standard are five items; Pleurotus ostreatus, Pleurotus eryngii, Flammulina velutipes, Agaricus bisporus and Ganoderma lucidum. But many farmers are suffering from a strict standards. So these standard is required modification to fit farmhouse situations. This study was carried out to investigate mushroom quality of farm shipping and quality change during preservation at various treatment. Flammulina velutipes and Agaricus bisporus preserved at different temperature( 4℃, 10℃, 20℃) to investigate quality changes. Pileus diameters of Flammulina velutipes was 67% of first grade and 33% of second grade at the early stage. After two weeks, pileus diameters was not signigicant changes; 50% of first grade, 50% of second grade at 4℃ and 50% of second grade, 50% of third grade at 10℃. Although there were no significant changes of diameter at 20℃, most of the fruit bodies were decayed. In case of Agaricus bisporus, pileus diameter was 15% of first grade, 81% of second grade and 4% of third grade at the early stage. The more storage period longed, the more the color of the fruit body was browned. But pileus diameters was not signigicant changes. Hardness and chromaticity of these mushroom was declined as time passed. Now we are carrying out more research on some mushroom’s standards for improve consumer confidence.

Expression of cellular retinoic acid-binding protein-I (CRABP-I) in the cerebrospinal fluid of adult onset moyamoya disease and its association with clinical presentation and postoperative haemodynamic change

Jeon, Jin Sue,Ahn, Jun Hyong,Moon, Youn-joo,Cho, Won-Sang,Son, Young-Je,Kim, Seung-Ki,Wang, Kyu-Chang,Bang, Jae Seung,Kang, Hyun-Seung,Kim, Jeong Eun,Oh, Chang Wan BMJ Publishing Group Ltd 2014 Journal of neurology, neurosurgery and psychiatry Vol.85 No.7

<P><B>Objective</B></P><P>The elevation of cellular retinoic acid-binding protein-I (CRABP-I) has been suggested as a candidate in the pathogenesis of paediatric moyamoya disease (MMD). However, few studies have addressed CRABP-I in adult onset MMD. The aim of this study was to examine the expression of CRABP-I in the cerebrospinal fluid (CSF) of adult onset MMD, and to evaluate its association with clinical presentation and postoperative haemodynamic change.</P><P><B>Methods</B></P><P>This study examined the CSF from 103 patients: bilateral MMD, n=58 (56.3%); unilateral MMD, n=19 (18.4%); atherosclerotic cerebrovascular disease (ACVD), n=21 (20.4%); and control group, n=5 (4.9%). The intensity of CRABP-I was confirmed by western blotting and expressed as the median (25th–75th percentile). The differences in CRABP-I expression according to disease entity (unilateral MMD vs bilateral MMD vs ACVD), initial presenting symptoms (haemorrhage vs ischaemia) and postoperative haemodynamic change (vascular reserve in single photon emission CT and basal collateral vessels in digital subtraction angiography) were analysed.</P><P><B>Results</B></P><P>CRABP-I intensities in bilateral MMD (1.45(0.86–2.52)) were significantly higher than in unilateral MMD (0.91(0.78–1.20)) (p=0.044) or ACVD (0.85(0.66–1.11)) (p=0.004). No significant differences were noted based on the initial presenting symptoms (p=0.687). CRABP-I was not associated with improvement in vascular reserve (p=0.327), but with decrease in basal collateral vessels (p=0.023) postoperatively.</P><P><B>Conclusions</B></P><P>Higher CRABP-I in the CSF can be associated with typical bilateral MMD pathogenesis in adults. Additionally, postoperative basal collateral change may be related to the degree of CRABP-I expression.</P>

계면활성제를 첨가한 TMA-포접화합물의 상변화에 관한 실험적 연구

김창오,정낙규,김진흥,오율권 朝鮮大學校 機械技術硏究所 2001 機械技術硏究 Vol.4 No.1

The purpose of this study is to investigate the propriety of TMA clathrate as a cold storage medium. Particularly, this is to examine the extent of subcooling improvement when the surfactants are added to the TMA clathrate, because water used cold storage material has low phase change temperature and subcooling. This study has been analyzed and compared with TMA 30wt% clathrate how phase change temperature, subcooling and specific heat in the various concentrations are changed. This results view to be improved phase change temperature and subcooling of TMA30wt% as a cold storage medium, when it had some surfactant. Additionally, it is that surfactant must be controlled under available solution limit and study for new additive must be lasted to know its effect.

TMA-포접화합물에 대한 계면활성제의 영향

김창오,정낙규,김진흥,오율권 朝鮮大學校 機械技術硏究所 2001 機械技術硏究 Vol.4 No.2

The purpose of this study is to investigate the propriety of TMA clathrate as a cold storage medium. Particularly, this is to examine the extent of subcooling improvement when the surfactants are added to the TMA clathrate, because water used cold storage material has low phase change temperature and subcooling. This study has been analyzed and compared with TMA 30wt% clathrate how phase change temperature, subcooling and specific heat in the various concentrations are changed. This results view to be improved phase change temperature and subcooling of TMA30wt% as a cold storage medium, then it had some surfactant. Additionally, it is that surfactant must be controlled under available solution limit and study for new additive must be lasted to know its effect.

에탄올이 첨가된 TMA포접화합물의 냉각특성

김진홍,정낙규,김창오,박호진 조선대학교 에너지.자원신기술연구소 2002 에너지·자원신기술연구소 논문지 Vol.24 No.2

The purpose of this study is to investigate the propriety of TMA clathrate as a cold storage medium. Particularly, this is to examine the extent of subcooling improvement when the additives are added to the TMA clathrate, because water used cold storage material has low phase change temperature and subcooling. This study has been analyzed and compared with TMA 30 wt% clathrate how phase change temperature, subcooling and specific heat in the various concentrations are changed. This results view to be improved phase change temperature and subcooling of TMA 30wt% as a cold storage medium, when it had some additive. Additionally, it is found that the additive must be controlled under available solution limit and study for new additive must be lasted to know its effect.

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

물-에너지-식량-탄소 넥서스를 이용한 통합물관리 모델 평가 연구 - 영산강 수계를 중심으로 -

나라 ( Na Ra ),박진현 ( Park Jin-hyeon ),주동혁 ( Joo Donghyuk ),김하영 ( Kim Hayoung ),유승환 ( Yoo Seung-hwan ),오창조 ( Oh Chang-jo ),이상현 ( Lee Sang-hyun ),오부영 ( Oh Bu-yeong ),허승오 ( Hur Seung-oh ) 한국농촌계획학회 2023 농촌계획 Vol.29 No.1

Active attention and effort are needed to develop an integrated water management system in response to climate change. In this study, it proposed models for cross-use of agricultural water and river maintenance water using sewage treatment water as an integrated water management system for the Yeongsan River. The impact of the integrated water management models was assessed by applying the concept of Nexus, which is being presented worldwide for sustainable resource management. The target year was set for 2030 and quantitatively analyzed water, energy, land use and carbon emissions and resource availability index by integrated water management models was calculated by applying maximum usable amount by resource. An integrated water management system evaluation model using the Nexus concept developed in this study can play a role that can be viewed in a variety of ways: security and environmental impact assessment of other resources. The results of this research will be used as a foundation for the field of in the establishment of a policy decision support system to evaluate various security policies, as we analyzed changes in other factors according to changes in individual components, taking into account the associations between water, energy, food, and carbon resources. In future studies, additional sub-models need to be built that can be applied flexibly to changes in the future timing of the inter-resource relationship components. 1)

APPLICATION OF TRAVELING SALESMAN PROBLEM(TSP) FOR DECISION OF OPTIMAL PRODUCTION SEQUENCE

Chang, Kun Soo,Yeo, Yeong Koo,Chang, Jin Yang,Kim, Kil Su,Jeong, Eun Young,Oh, Sea Cheon 한국화학공학회 1997 Korean Journal of Chemical Engineering Vol.14 No.5

In the present study a reliable and structural decision system for production sequence of polymeric products is developed. Minimization of the amount of off-specs are the main objective in the decision of production sequence to maximize profit. Off-specs are generated when the production sequence of polymeric products is changed. The amount of off-specs depends on changes of product grades. In the present study we applied the traveling salesman problem (TSP) to achieve optimal decision of production sequence. To solve the optimal decision problem formulated by TSP, we employed three different approaches such as Branch and Bound (B&B) method. Dynamic Programming (DP) method and Hopfield Neural Network (HNN) method. Production sequences computed based on the actual plant off-spec data were compared with the sequences employed in the actual plant operation. From the comparison the decision method proposed in the present study showed increased profits and reduced off-specs.

오징어젓 숙성 중 N-nitrosamine 생성에 관한 연구

오창경,김수현,송대진,오명철,김성수 濟州大學校工科大學産業技術硏究所 1996 尖端技術硏究所論文集 Vol.7 No.1

본 연구는 오징어에 식염 20%를 첨가하는 고식염구와 식염 10%에 솔비톨, 에탄올, 젖산 등을 첨가한 저식염구로 나누어 오징어젓을 제조한 후, 이들을 숙성시키면서 N-nitrosamine의 생성 여부와 숙성 중 그의 전구물질인 아질산염과 질산염, DMA 및 TMA와 TMAO의 변화를 검토하였다. 오징어젓 숙성 중 pH는 저식염구가 5.79∼6.07, 고식염구가 6.05∼6.21 사이에서 변화하였다. 아질산염질소와 질산염질소는 각각 0.75∼1.04mg/kg 및 1.22∼1.42mg/kg 사이에서 변화하였다. TMA-N는 숙성 중 계속하여 증가하여 숙성 140일에는 고식염구가 20.1mg%, 저식염구가 14.9mg%였으며, TMAO-N는 생시료에서 27.9mg%이었던 것이 숙성 중기까지 완만하게 증가하다가 그 이후에는 다시 감소하였다. DMA-N는 숙성 중 계속하여 증가하였으며 숙성 140일에는 고식염구는 19.7mg/kr, 저식염구는 13.3mg/kg이었다. 오징어젓의 N-nitrosamine으로는 NDMA만이 검출되었고, 숙성 40일째에 저식염구에 흔적량, 고식염구에 3.8㎍/kg의 양으로 생성되었으며 숙성 중 계속해서 증가하여 57.5∼74.7㎍/kg에 이르렀다. Possibility of formation of carcinogenic N-nitrosamines and changes in their precursors during the fermentation of high salt-fermented squeid, Sepiell maindroni, group with 20% of salt and low salt-fermented squid group with 10% of salt, 6% of ethanol, 6% of sorbitol and 0.5% of lactic acid were investigated. pH during the fermentation of squid changed in the ranges of 5.79∼6.07 and 6.05∼6.21 in low and high salt group, respectively. The contents of nitrite- and nitrate-N during the fermentation were in the ranges 0.75∼1.04 and 1.22∼1.42mg/kg, respectively. Trimethylamine(TMA) was continuously increased during the fermentation and showed 20.1 and 14.9mg% in high and low salt group after 140 days of the fermentation, respectively, while trimethylamine oxide(TMAO) was gradually increased from the beginning until the middle period of fermentation and then was again decreased. Dimethylamine(DMA) was continuously increased during the fermentation and showed 19.7 and 13.3mg/kg in high and low salt group after 140 days of the fermentation, respectively. N-nitrosamines in salt-fermented squid was detected only N-nitrosodimethylamine(NDMA), which its amount was trace in low salt group and 3.8㎍/kg in high salt group on 40 days of the fermentation and then continuously increased in the ranges of 57.5∼74.7㎍/kg in both groups during the fermentation.