Effects of Phenytoin and Diazepam on the Seizure Activity in the Cortical Dysplasia Animal Models

Kim, Si-Hyung,Choi, In-Sun,Cho, Jin-Hwa,Park, Eun-Ju,Jang, Il-Sung,Choi, Byung-Ju,Kim, Hyun-Jung,Kim, Young-Jin,Nam, Soon-Hyeun The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Dysplasia-associated seizure disorders are markedly resistant to pharmacological intervention. Relatively little research has been conducted studying the effects of antiepileptic drugs(AEDs) on seizure activity in a rat model of dysplasia. We have used rats exposed to methylazoxymethanol acetate(MAM) in utero, an animal model featuring nodular heterotopia, to investigate the effects of AEDs in the dysplastic brain. Pilocarpine was used to induce acute seizure in MAM-exposed and age-matched vehicle-injucted control animals. Field potential recordings were used to monitor amplitude and numbers population spikes, and paired pulse inhibition in response to stimulation of commissural pathway. Two commonly used AEDs were tested: diazepam 5, 2.5mg/kg;phenytoin 40, 60mg/kg. Diazepam(DZP) and phenytoin(PHT) reduced the amplitude of population spike in control and MAM-exposed rats. However, the amplitude of population spike was nearly eliminated in control rats as compared to the MAM-exposed rats. Pharmaco-resistance was tested by measuring seizure latencies in awake rats after pilocarpine administration(320mg/kg, i.p.) with and without pretreatment with AEDs. Pre-treatment with PHT 60 mg prolonged seizure latency in control rats, but not in MAM-exposed animals. The main findings of this study are that acute seizures initiated in MAM-exposed rats are relatively resistant to standard AEDs assessed in vivo. These data suggest that animal model with cortical dysplasia can be used to screen the effects of potential AEDs.

백서에서 Depulpin®과 Formocresol에 대한 치수와 치근단 조직의 반응

문형인,김선호,황윤찬,오병주,황인남,김선헌,정선와,윤창,오원만 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.4

One fifth dilution of formocresol is usually for pulpotomy of the primary teeth and emergency pulpotomy of the permanent teeth. However, the use of formaldehyde has been subjected to criticism because it may be absorbed into the blood stream and become distributed systemically, it may also alter the pulp tissue rendering it immumologically active, and have carcinogenic potential. Recently Depulpin®(VoCo., Germany) gains popularity as a devitalizing agent during root canal therapy in spite of high concentration of 49% paraformaldehyde because it facilitate devitalization of pulp and make root canal therapy easier. But there have been not enough publications about the reaction of pulp and periapical tissue caused by Depulpin. This study was performed to evaluate the histological changes in pulp and periapical tissue of rats after pulpotomy using formocresol and Depulpin and to elucidate the toxic effects of these agents. Thirty six Sprague-Dawley rats were anesthetized by intraperitoneal injection of ketamine. Maxillary first molar teeth were used for pulpotomy with formocresol and Depulpin. Rats were sacrificed after 2 days, 4 days, 1 week, 2 weeks, 3 weeks and 4 weeks respectively. Specimens were histologically observed by light microscope changes in pulp and periapical tissue. The obtained results were as follows. 1. Formocresol group A zone of fixed tissue, in which odontoblasts could clearly be defined, was present directly underneath the pulpotomy dressing in almost all teeth of this group. This was followed by an area of necrotic tissue which resembled dried out fibrous tissue with no cellular detail except some pyknotic nuclei. In the specimens of after 2 days, 4 days, 1 week, 2 weeks in which vital tissue was present. it was separated from the fibrous area by a zone of inflammation. In the specimens of after 3 weeks and after 4 weeks, inflammatory infiltrate was in the periodontal ligament adjacent to the apical foramina of the teeth. 2. Depulpin® group The area of necrotic tissue which had no cells and fibers , was present adjacent to the dressing. This was followed by dried out fibrous tissue with no cellular details except some pyknotic nucleli. A short stump of vital pulp with odontoblasts was present at the end of the canal after 2 days. Inflammatory infiltrate was in the periodontal ligament after 4 days and after 1week. Severe root resorption and necrosis of periapical tissue opposite the root resorption site were defined after 2 weeks and after 3 weeks. Periapical lesion which consist of necrotic tissue surrounded by a fibrous connective wall. was found after 4 weeks. The results indicated that Depulpin can cause more adverse reaction to the dental pulp and periapical tissue than formocresol, and further studies are needed for its clinical use with safety.

백서에서 Depulpin^�과 Formocresol에 대한 치수와 치근단 조직의 반응

문형인,황인남,오원만 전남대학교 치의학연구소 2002 구강과학 Vol.14 No.2

One fifth dilution of formocresol is usually used for pulpotomy of the primary teach and emergency pulpotomy of the permanent teeth. However, the use of formaldehyde has been subjected to criticism because it may be absorbed into the blood stream and become distributed systemically, it may also alter the pulp tissue rendering it immunologically active, and have carcinogenic potential. Recently Depulpin^(r)(VoCo., Germany) gains popularity as a devitalizing agent during root canal therapy in spite of high concentration of 49% paraformaldehyde because it facilitate devitalization of pulp and make root canal therapy easier. But there have been not enough publications about the reaction of pulp and periapical tissue caused by Depulpin. This study was performed to evaluate the histological changes in pulp and periapical tissue of rats after pulpotomy using formocresol and Depulpin and to elucidate the toxic effects of these agents. Thirty six Sprague-Dawley rats were anesthetized by intraperitoneal injection of ketamine. Maxillary first molar teeth were used for pulpotomy with formocresol and Depulpin. Rate were sacrificed after 2days, 4days, 1week, 2weeks, 3weeks and 4weeks respectively. Specimens were histologically observed by light microscope changes in pulp and periapical tissue. The obtained results were as follows. 1. Formocresol group A zone of fixed tissue, in which odontoblasts could clearly be defined, was present directly underneath the pulpotomy dressing in almost all teeth of this group. This was followed by an area of necrotic tissue which resembled dried out fibrous tissue with no cellular detail except some pyknotic nuclei. In the specimens of after 2days, 4days, 1week, 2weeks in which vital tissue was present, it was separated from the fibrous area by a zone of inflammation. In the specimens of after 3weeks and after 4 weeks, inflammatory infiltrate was in the periodontal ligament adjacent to the apical foramina of the teeth. 2. Depulpin^(r) group The area of necrotic tissue which had no cells and fibers, was present adjacent to the dressing. This was followed by dried out fibrous tissue with no cellular details except some pyknotic nuclei. A short stump of vital pulp with odontoblasts was present at the end of the canal after 2days. Inflammatory infiltrate was in the periodontal ligament after 4days and after 1week. Severe root resorption and necrosis of periapical tissue opposite the root resorption site were defined after 2 weeks and after 3weeks. Periapical lesion which consist of necrotic tissue surrounded by fibrous connective wall, was found after 4weeks. The results indicated that Depulpin can cause more adverse reaction to the dental pulp and periapical tissue than formocresol, and further studies are needed for its clinical use with safety.

Feasibility and Effects of a Postoperative Recovery Exercise Program Developed Specifically for Gastric Cancer Patients (PREP-GC) Undergoing Minimally Invasive Gastrectomy

Cho, In,Son, Younsun,Song, Sejong,Bae, Yoon Jung,Kim, Youn Nam,Kim, Hyoung-Il,Lee, Dae Taek,Hyung, Woo Jin The Korean Gastric Cancer Association 2018 Journal of gastric cancer Vol.18 No.2

Purpose: Exercise intervention after surgery has been found to improve physical fitness and quality of life (QOL). The purpose of this study was to investigate the feasibility and effects of a postoperative recovery exercise program developed specifically for gastric cancer patients (PREP-GC) undergoing minimally invasive gastrectomy. Materials and Methods: Twenty-four patients treated surgically for early gastric cancer were enrolled in the PREP-GC. The exercise program comprised sessions of In-hospital Exercise (1 week), Home Exercise (1 week), and Fitness Improvement Exercise (8 weeks). Adherence and compliance to PREP-GC were evaluated. In addition, body composition, physical fitness, and QOL were assessed during the preoperative period, after the postoperative recovery (2 weeks after surgery), and upon completing the PREP-GC (10 weeks after surgery). Results: Of the 24 enrolled patients, 20 completed the study without any adverse events related to the PREP-GC. Adherence and compliance rates to the Fitness Improvement Exercise were 79.4% and 99.4%, respectively. Upon completing the PREP-GC, patients also exhibited restored cardiopulmonary function and muscular strength, with improved muscular endurance and flexibility (P<0.05). Compared to those in the preoperative period, no differences were found in symptom scale scores measured using the European Organization for Research and Treatment of Cancer (EORTC) Core Quality of Life Questionnaire (QLQ-C30) and Quality of Life Questionnaire-Stomach Cancer-Specific Module (QLQ-STO22); however, higher scores for global health status and emotional functioning were observed after completing the PREP-GC (P<0.05). Conclusions: In gastric cancer patients undergoing minimally invasive gastrectomy, PREP-GC was found to be feasible and safe, with high adherence and compliance. Although randomized studies evaluating the benefits of exercise intervention during postoperative recovery are needed, surgeons should encourage patients to participate in systematic exercise intervention programs in the early postoperative period (Registered at the ClinicalTrials.gov, NCT01751880).

Long Term Prognostic Value of HbA1C in Acute Myocardial Infarction Patients without Known Diabetes

( Il Hyung Jung ),( Chung Kang ),( Dong In Nam ),( Sang Cheol Cho ),( Wan Kim ),( Myung Ho Jeong ) 대한내과학회 2013 대한내과학회 추계학술발표논문집 Vol.2013 No.1

Background: The prognostic role of glycosylated hemoglobin (HbA1C) in non-diabetic (non-DM) patients with acute myocardial infarction (AMI) is still controversial. We evaluated the prognostic role of HbA1C in AMI patients without previously known diabetes. Methods: From January 2008 to August 2011, 3,292 in AMI patients with non-DM [65.6±13.1 years, 2,460 men (74.8%)] who underwent laboratory testing of HbA1C were enrolled retrospectively. Patients were divided into two groups by HbA1C [Group 1 ≤ 6.5%, n=2,864 (87%); Group 2 > 6.5%, n=428 (13%)]. The primary end point was composite major adverse cardiac events (MACEs) including all cause death, cardiac death, re-MI, and revascularization of target or non-target vessel during 12 months follow up. Results: Patients of in-hospital all cause death and cardiac death were 202 [169 (6.0%) vs. 33 (6.8%), p>0.05) and 145 [124 (4.4%) vs. 21 (4.3%), p>0.05], respectively. BMI (24.0±3.1 vs. 24.9±3.6), blood glucose (145±55 vs. 207±90 mg/dL), total cholesterol (184±44 vs. 196±47 mg/dL), triglyceride (121±88 vs. 157±118 mg/dL), LDL (118±39 vs. 127±41 mg/dL) and hs-CRP (5.35±20.3 vs. 8.76±34.9 mg/dL) were higher and HDL (44.4±14.7 vs. 42.2±11.7 mg/dL) was lower in group 2 (p of all variables < 0.01). The incidence of hyperlipidemia (9.2% vs. 12.4%, p=0.036), obesity (BMI ≥ 30, 3.3% vs. 8.2%, p<0.01), current smoker (44.7% vs. 50.2%, p=0.032), and left ventricular (LV) dysfunction (ejection fraction<40%, 11.3% vs. 15.4%, p=0.021) were more frequent in group 2. There were no significant difference in clinical outcomes at 12 months. In subgroup analysis (group 1 was divided by 5.5% of HbA1C), there were no significant difference in short and long term clinical outcomes. Old Age (age ≥ 65 years), high Killip class (III & IV), LV dysfunction, and renal insufficiency (GFR <60 mL/min) were independent predictors of 12 months composite MACEs. Conclusion: Korean AMI patients with non-DM and HbA1C > 6.5% were not associated with worse outcomes during 12 months follow up.

Anthracycline제재로 치료받은 종양환아에서 QT분산

고재곤,김영휘,박인숙,서종진,문형남 대한소아과학회 2001 小兒科 Vol.44 No.8

Hi-fi오디오 전송용 무선 송.수신기 설계

정인화,남옥우,김재형 창원대학교 정보통신연구소 2001 精報通信論文集 Vol.5 No.-

In this paper, a high-fidelity wireless microphone system with 16 channels in UHF band have been designed and implemented. According to the table of radio frequency allocation, a frequency for the wireless microphone is allocated in radio band from 740MHz to 752MHz and maximum frequency deviation is ±75KHz. The wireless microphone system have been designed with frequency modulation, PLL(Phase Locked Loop) frequency synthesized method, dual conversion, preemphasis/deemphasis and commander of 2:1 type. The wireless microphone system have been designed in this study has a frequency response from 50 to 20,000Hz and THD(Total Harmonic Distortion) lower then 1%. A high-fidelity audio can be transmitted through the air by the designed wireless microphone system Kevword'UHF Wireless Microphone, PLL, FM.

녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

혈우병 환자의 임상 양상에 관한 단일기관 연구

허지혜,김형욱,김영대,이영호,김남수,설인준,김상우 인제대학교 2006 仁濟醫學 Vol.27 No.-

Objective : We analyzed and investigated clinical manifestations, laboratory tests, and inhibitor development of hemophilia that had not been thoroughly studied. Methods : We retrospectively reviewed 96 patients with hemophilia who were registered in the Hanyang University hospital from Jan, 1984 to Dec, 2003, Results : The total patients, whose median age at onset was 46.0 months, in clude 84 cases of type A (87.5%) and 12 cases of type B (12.5%). The most common chief complaint was a traumatic hemorrhage. The family histories of hemophilia were revealed in 41 cases (42.7%). The median activated partial thromboplastin time (aPTT) was 66.7 seconds. The number of severe cases was 39 (40.6%). Inhibitors were present in 13 cases (13.5%) and seven cases (53.8%) were high responders. aPTT and factor levels showed reverse correlation (r=-0.467). aPTT is more prolonged in group without family history than the group with family history (P=0.037). Conclusion : In this study, the diagnosis of hemophilia are made at the lower age and the serological positivity of the HCV is decreased. Activated partial thromboplastin time and factor levels shows reverse correlation. Activated partial thromboplastin time is more prolonged in group without family history compared with group with family history. The frequencies of family history are lower than those of other developed countries, because of concealing the family history of hemophilia. The prevalence of the development of inhibitors increased compared with those of previous studies in Korea, but was lower than those of other countries. Further studies would be necessary to decrease the inhibitor development.

노인체육지도자들의 코로나에 대한 인식과 경험에 관한 연구

권기남(Kwon, Ki-Nam),김인형(Kim, In-Hyung),박성혜(Park, Seong-Hye) 한국스포츠사회학회 2022 한국스포츠사회학회지 Vol.35 No.4

본 연구의 목적은 노인체육지도자들의 COVID-19에 대한 인식에 대해 알아보고, 변화된 지도환경에서 어떠한 어려움을 겪고 있는지 그 경험을 탐색하는 것이다. 이러한 연구목적을 달성하기 위해 현상학 연구를 실시하였다. 연구참여자는 유목적 표집법을 이용하여 D광역시 L, A구체육회, H 및 J제공기관, N노인회, K복지관의 소속 지도자들 12명을 선정하였다. 자료는 참여관찰, 심층면담, 관련 자료 수집을 통해 수집하였다. 이러한 자료를 토대로 분석한 연구결과는 다음과 같다. 첫째 노인체육지도자들이 정부가 단계적 일상회복을 위해 폐쇄되었던 체육시설을 개방하게 되면서 지도현장으로 다시 돌아오게 되었지만, COVID-19가 종식되지 않은 상황에서 COVID-19는 감염에 대한 불안감과 죽음을 가져오는 공포의 대상이었다. 이러한 인식은 백신만이 COVID-19를 종식시킬 수 있다고 믿고 백신을 접종함으로 COVID-19에 대한 안도하는 마음을 갖게 되면서 변화되었다. 그러나 COVID-19가 장기화되면서 공포의 대상이 아닌 함께 공존하는 대상으로 인식하게 되었다. 둘째, 노인체육지도자들은 COVID-19 발생 초기 한명의 확진자만 발생해도 감염 확산의 위험으로 시설이 폐쇄되는 상황을 겪어야 했기 때문에, 감염에 대한 불안한 마음으로 긴장된 삶을 살아가는 어려움을 겪고 있었다. 또 사회적 거리두기와 마스크 착용으로 수업을 지도하는데 소통의 어려움을 겪었으며, COVID-19에 대한 방역과 관리하는 등 지도자로서의 역할이 가중됨으로 어려움을 겪고 있는 것으로 나타났다. The purpose of this study is to find out the perception of COVID-19 at the time of occurrence and the perception of elderly sports leaders about COVID-19 in the current situation that two years have passed, and to explore with in-depth experience of what kind of difficulties they are experiencing in the changed guidance environment. For this, an ethnography study was conducted. The participants were selected using a purposeful sampling to collect 12 leaders from L and A district sports associations, H and J provider organizations, N elderly associations, and K. Data were collected through participatory observation, in-depth interviews, and related data collection. The results are as follows. First, it was brought back to the guidance environment as the government opened the sports facilities that had been closed for a step-by-step recovery of daily life, but in a situation where COVID-19 has not ended, COVID-19 has been the subject of anxiety about infection and the fear of death. This perception has changed as they believe that only a vaccine can end COVID-19, and that giving them a vaccine gives them relief for COVID-19. However, as COVID-19 continues for a long time, it has come to be recognized as an object of coexistence rather than an object of fear. Second, in the early stages of the COVID-19 outbreak, even if there was only one confirmed patient, the facility had to be closed due to the risk of spreading the infection, so it was difficult to live a tense life with an anxious mind about the infection. Also, social distancing and wearing a mask made it difficult to communicate while teaching classes. They were experiencing difficulties as their role as a leader increased, such as quarantine and information collection for COVID-19.