A selective cyclin-dependent kinase 4, 6 dual inhibitor, Ribociclib (LEE011) inhibits cell proliferation and induces apoptosis in aggressive thyroid cancer

Lee, Hyun Joo,Lee, Woo Kyung,Kang, Chan Woo,Ku, Cheol Ryong,Cho, Yoon Hee,Lee, Eun Jig Elsevier 2018 Cancer letters Vol.417 No.-

<P><B>Abstract</B></P> <P>The RB-E2F1 pathway is an important mechanism of cell-cycle control, and deregulation of this pathway is one of the key factors contributing to tumorigenesis. Cyclin-dependent kinases (CDKs) and Cyclin D have been known to increase in aggressive thyroid cancer. However, there has been no study to investigate effects of a selective CDK 4/6 inhibitor, Ribociclib (LEE011), in thyroid cancer. Performing Western blotting, we found that RB phosphorylation and the expression of Cyclin D are significantly higher in papillary thyroid cancer (PTC) cell lines as well as anaplastic thyroid cancer (ATC) cell lines, compared with normal thyroid cell line and follicular thyroid cancer cell line. LEE011 dose-dependently inhibited RB phosphorylation and also decreased the expressions of its target genes such as <I>FOXM1, Cyclin A1,</I> and <I>Myc</I> in ATC. Furthermore, LEE011 induced cell cycle arrest in G0-G1 phase and cell apoptosis, and inhibited cell proliferation in ATC. Consistently, oral administration of LEE011 to ATC xenograft models strongly inhibited tumor growth with decreased expressions of pRB, pAKT and Ki-67, and also significantly increased tumor cell apoptosis. Taken together, our data support the rationale for clinical development of the CDK4/6 inhibitor as a therapy for patients with aggressive thyroid cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> pRB and Cyclin D were expressed high in aggressive thyroid cancer. </LI> <LI> LEE011 suppressed pRB and also decreased the expressions of its target genes in ATC. </LI> <LI> LEE011 induced cell cycle G1 arrest and apoptosis, and inhibited cell proliferation. </LI> <LI> LEE011 inhibited in vivo tumor growth with decreased expressions of pRB and Ki-67. </LI> <LI> We could explain the anticancer effects with the RB-E2F pathway. </LI> </UL> </P>

항인지질 항체 증후군에서 항β₂glycoprotein-Ⅰ 항체의 의미

이찬희,서창희,이지수,이수곤 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

objective : This study investigated the significance of anti-β₂ glycoprotein-Ⅰ(β₂GPI) antibody in antiphospholipid syndrome from the results of the relationship between the antiphospholipid antibody test and the anti-β₂GPI antibody test. Methods : The IgM, IgG type of the anti-β₂GPI antibody was tested by ELISA in patients with antiphospholipid syndrome or those who were suspected of having antiphospholipid syndrome and compared with the anticardiolipin antibody and lupus anticoagulant results. As a control, the anti-β₂GPI antibody was tested in 12 normal healthy persons and 8 systemic lupus erythematosus patients. Results : In 6 patients with antiphospholipid syndrome, the positive rate of lupus anticoagulant, IgM and IgG anticardiolipin antibody was 33%, 50%, and 67%. However, the positive rate of the IgM and IgG anti-β₂GPI antibody test was 83%, 100%. Among the 6 patients, 3 patients were tested for the anti-β₂GPI antibody before and after treatment. In all 3 patients, the anti-β₂GPI antibody titer was lower after treatment. The anti-β₂GPI antibody test was positive in those patients suspected for having antiphospholipid syndrome, but tested negative to the anticardiolipin antibody or lupus anticoagulant. In the normal and disease controls, the anti-β₂GPI antibody test was negative in all cases. Conclusion : In diagnosing antiphosphotipid syndrome. the anticardiolipin antibody or lupus anticoagulant should be used as a screening test, and if necessary, an anti-β₂GPI antibody should be added. This might improve the sensitivity and specificity. Also, the anti-β₂GPI antibody titer may be used as a marker of disease activity.

Transmission ability of Zika virus with artificially infected Aedes albopictus in Korea

Yang Sung‐Chan,Lee Hee-Il,Kim Hyunwoo,Lee Wook‐Gyo 한국곤충학회 2021 Entomological Research Vol.51 No.8

Zika virus (ZIKV), a virus mainly occurring in South America, is now globally distributed. Understanding the pathogen transmission cycle in a vector is exceptionally important in developing disease control strategies. In this study, we performed ZIKV microinjection in Aedes albopictus (Skuese), to estimate its vertical and horizontal transmission ability. The virus infection rate was confirmed by real-time qPCR in the F1 generation derived from the ZIKV-injected Ae. albopictus. The minimum infection rate (MIR) of the F1 generation was highest when they were injected with the virus at 6–12 h (MIR: 16.1) and lowest at 12–24 h (MIR: 4.7) after feeding. In the developmental stage of the F1 progeny, MIR values were 1.0, 1.3, and 6.7 in each egg, larval, and adult stage, respectively, but ZIKV was not detected in the pupa stage. Virus transmission ability was not significantly different between the collection areas (Tongyeong and Jeju). Ae. albopictus demonstrated a high venereal transmission rate of ZIKV and was detected in males (6 pool/7 pool) and females (6 pool/7 pool), confirming that ZIKV can be transmitted from infected mosquitoes to uninfected mosquitoes via mating. This is the first study of the administration of a ZIKV microinjection to Ae. albopictus in Korea and suggests a possibility of a potential mechanism for the virus to survive during adverse conditions via vertical transmission.

B16 멜라닌 세포에서 해양소재 추출물의 멜라닌 생성 저해 효과

이찬 ( Chan Lee1 ),장정희 ( Jung Hee Jang ),안은미 ( Eun Mi Ahn ),박찬익 ( Chan Ik Park ) 대한본초학회 2012 大韓本草學會誌 Vol.27 No.4

Objectives : Under normal condition melanin protects the skin from extracellular stimuli including ultraviolet (UV)-induced oxidative skin damages, but excess production and accumulation of melanin can induce hyperpigmentation causing esthetic problems. Therefore, in this study we tried to search for natural skin whitening materials from marine natural resources. Methods : Water and ethanol extracts of marine natural resources were prepared from Porphyra thalli (PT), Laminariae thallus (LT), Ostreae concha (OC), Sargassum thallus (ST), Undaria thallus (UT), Codium thalli (CT), Enteromorpha thalli (ET), Syngnathoides biaculeatus (SB), and Hippocampus coronatus (Hc). Their effects against UVB and α-melanocyte stimulating hormone (α-MSH)-induced melanogenesis were investigated based on melanin formation in B16 mouse melanoma cells. The mRNA and protein expression of enzymes involved in the melanogenic process were further examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. Results : Water extract of Ostreae concha (OCW/E) effectively inhibited UVB and α-MSH-induced melanin production in B16 melanocytes, which seemed to be mediated by inhibition of mRNA expression of tyrosinase and tyrosinase-related protein 1 (TRP-1). In another experiment, ethanol extracts from Porphyra thalli (PTE/E), Laminariae thallus (LTE/E), Sargassum thallus (STE/E), Undaria thallus (UTE/E), Codium thalli (CTE/E), Syngnathoides biaculeatus (SBE/E), and Hippocampus coronatus (HcE/E) significantly suppressed UVB and α-MSH-induced melanin formation. Furthermore, ethylacetate fraction isolated form LTE/E (LTE/EEt) decreased UVB and α-MSH-elevated extracellular melanin levels via inhibition of tyrosinase protein expression. Conclutions : These results suggest that marine natural resources such as Porphyra thalli, Laminariae thallus, Ostreae concha, Sargassum thallus, Undaria thallus, Codium thalli, Syngnathoides biaculeatus and Hippocampus coronatus have anti-melanogenic effects, thereby exhibiting high potentials to be utilized as one of the ingredients for the development of new whitening functional cosmetics.

스포츠種目別運動選手의 體格, 體型 및 體組成에 관한 硏究

李相于,趙在基,朴贊熙,朴俊東,辛尙根 東亞大學校附設스포츠科學硏究所 1989 스포츠科學硏究論文集 Vol.7 No.-

이온교환법에 의한 탈질소 공정개발의 기초연구 (Ⅴ) : 질산성 질소 선택적인 이온교환수지의 합성 Synthesis of Nitrate-Selective lon Exchange Resines

이동환,김승일,전진희,박찬영,이민규 한국환경과학회 2000 한국환경과학회지 Vol.9 No.4

Nitrate-selective ion exchange resin which have bulky tertiary amine as functional group have been synthesized by the reaction of chloromethylated polystyrene-divinylbenzene copolymer and the corresponding tertiary amine [NR_3=NEt_3 1, N(C_2H_4OH)_3 2] in ethanol, while commercial resin has NMe_3 as functional group. The fundamental properties such as bulk density, water content, appearance index, exchange capacity, effective size, uniformity coefficient of synthesized anion exchange resin (1) have been measured. The ion exchange resin (1) and (2) exhibited the better selectivity for nitrate than sulfate in both batch and continuous column experiments.

산간 계곡 지역 경지정리 사업의 평가

이창수,박승기,양승희 공주대학교 자원과학연구소 2000 資源科學硏究論文集 Vol.8 No.-

This study has appraised land consolidation project in the mountain stream region by way of the appraisement model of Potential Productivity Index (PPI) using GIS. PPI will provide basic elements for large-scale gathering of farm land and a substitute lot of farm land consolidation, and will maximize productivity of paddy. This study was carried out to determine relative error and correlative analysis for verifiable fitness of using cadastral map GIS DB compares determined area using GIS DB with protocol area at each project region. NPI was determined by overlapping Poly Grid of land properties in analyzed project region. NPI of the Masu region was 0.882 TPI that was estimated by additional productive wages ratio of the total of direct productive cost. Additional productive wages were determined by GIS Network analysis of the working distance from each farm house to paddy. PPI was determined at each analyzed farm house and paddy by weighted NPI and TPI. NPI'S and TPI'S weights for PPI were 80%, 20% respectively. PPI variation of each analyzed paddy of the Masu region was 0.967~0.712, and could show the relative PPI value.

함안 방어산 마애삼존불의 보존처리를 위한 암석학적 연구

이찬희,서만철,최석원 공주대학교 문화재보존과학연구소 2003 문화재과학기술 Vol.2 No.1

방어산 마애삼존불의 풍화와 훼손도를 진단하였으며, 보존처리를 위한 암석학적 연구를 수행하였다. 이 마애불의 기반암은 니질기원의 퇴적암이 접촉변성을 받아 생성된 치밀견고한 청회색 호온펠스이다. 마애불 주변의 풍화면은 암갈색에서 황갈색을 보이나, 삼존불은 적갈색 또는 암흑색으로 갖는 것으로 보아 인위적 및 자연적인 훼손이 심각한 것으로 나타났다. 본존불 좌중단에는 음각선이 사라지고 없으며 암상의 변색 및 암편의 박락에 따른 계단식 풍화도 관찰된다. 마애불의 표면과 암석의 공극에는 선태류, 조류 및 지의류의 모근과 포자류가 기생하고 있으며, 절리대에서는 이미 토양화의 진행에 의하여 잡초와 잡목이 서식하고 있다. 따라서 식생과 표면의 오염물질을 제거하기 위한 세정작업이 있어야 할 것이다. 또한 마애불 본체의 구조적 안정을 위해 주변에 분포하는 불안정한 암괴의 제거가 필수적이며, 불균형의 재발생과 전면으로 발생하는 응력을 최소화하기 위한 암석지지공법이 필요하다. 균열이 심한 절리면에는 암석용 충전제를 이용한 보존처리가 가해져야 하며, 마애불에 미치는 기상변화를 저감하기 위한 차수벽과 보호각의 설치를 제안한다. The rock standing sculptured Triple Buddha of the Bangeo mountain, Korea was studied on petrological weathering and deterioration diagnosis for the future conservational treatments. Host rock of this Triple Buddha is bluish grey hornfels originated muddy sedimentary rock, which rock is very hard and compact due to the contact metamorphism. Weathered rock surface around the Triple Buddha is yellowish to dark brown color, but main Triple Buddha surface shows reddish brown to dark black because of the severely artificial and natural deteriorations. In the central left-side of the main Buddha, the carved lines are eliminated by weathering, and well developed step-like exfoliations and discoloring of lithology. Rock surface of the Triple Buddha shows partly covered with green algae and lichen. The joint planes occur some weeds and trees. This biological weathering and contaminations need for conservational cleaning and chemical treatments. The unstable rock block around the Triple Buddha most be to remove for the structural stability from the main body, and need to geotechnical methods for the minimum stress against the frontal toppling and reoccurrence of instability. For the conservation of the Triple Buddha, we suggest that fracture systems in the host rock should be to reinforce by petro-epoxy treatments, and set up a water curtain and protection house for attenuation of climatic changes.

우리나라 南西海岸의 海陸風 探究 : Centering around the city of Mokpo and Seochon

李承姬,柳贊洙 조선대학교 사범대학 부설 교과교육연구소 2000 敎科敎育硏究 Vol.3 No.1

Geographically, Three sides of Korea are surrounded by the sea(that is, the Korean Peninsular). Accordingly, the envioronment of our country has been greatly affected by the sea. For this study and taking an observation with AWS. I've selected the Mokpo city to which Taebul industrial complex is adjacent and Seochon, the central part of the western coast to which Asan industrial complex is adjacent. I've utilized the data on the change of the direction and the speed of the breeze of two areas(Mokpo and Seochon) through classifying all the data of the annual breeze into a daily unit, a monthly unit and a seasonal unit. As a result, there was a more vivid sea and land breeze character in Seochon area than in Mokpo area is due to the Mokpo's geographical location. In the frequency of breeze direction, NNW direction which occupied 27% of the whole was prevailing and in the frequency of breeze speed, the range of 0.3∼3.3㎧ appeared frequently, and it showed 48.9%. According to rarity analysis on the relations between breeze direction and breeze speed, a correlation coefficient was 0.56∼0.91 and through it. I could figure out correlation of breeze direction and breeze speed. But iti is matter of regret that this study has a statistical analytic basis mainly, so, from noe on, I intend to do profound research on factors which cause the change of sea and land breeze and I'd like to develop them to be processed as enviornmental factors.

슬개골 골절의 임상적 고찰

이준규,안상로,박찬희 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

We studies the cases of 30 patients who had fracture of patella, 28 patients were male and 2 patients were female. We treated the patients with operative treatment in 27 patients and with nonoperative treatment in 3 patients. The results are as follows. 1. It was more frequent in male, and average age of patient was 38.5 years old. 2. Most frequent cause of fractures was traffic accident. 3. Open fracture was 76.7%, and transverse or bursting fracture was 66.7%. 4. The end result was dependent on the initial trauma. 5. The associated injuries were frequent nearby the knee joints.