정신 분열증 환자의 말초혈액림프구에서 BrdU에 의해 유도되는 Fragile Sites

박경란 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

The expression frequency of BrdU-induced fragile sites was examined in schizophrenia patients to determine whether this parameter could be used as a marker of genetic susceptibility in at-risk individuals. No difference was found in expression frequency between the schizophrenia patients and a group of normal individuals. This indicates that the expression frequency of bromodeoxyuridine induced fragile sites is not a suitable marker for assessing genetic susceptibility in schizophrenia.

정신분열증 환자의 말초임파구에서 Fluorodeoxyuridine에 의해 유도되는 Fragile Sites

박경란,김원식 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

The expression frequency of FUdR-induced fragile sites was examined in schizo phrenia patients to determine whether this parameter could be used as a marker of genetic susceptibility in at risk individuals. No difference was found in expression frequency between the schizophrenia patients and a group of normal individuals. This indicates that the expression frequency of fluorodeoxyuridine induced fragile sites is not a suitable marker for assessing genetic susecptibility in schizophrenia.

Methotrexate가 脾臟에 미치는 影響에 관한 硏究

朴景蘭,李永浩,金源式 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

In order to study the morphological changes of spleen after treatment with methotrexate, ICR mice were used as experimental animal. The experimental mice were divided into control, 50mg/kg and 500mg/kg of methotrexate treated groups. Methotrexate injected intravenously via tail vein once on the 8th day of pregancy. The mice were sacrificed on the 1st, 2nd 3rd and 4th day after treatment, the spleens were removed and fixed in 10% neutral buffered formalin solution for 48hrs. Spleens were cutted 5㎛ thick and stained with H-E, then observed under the light microscope. The results were as follows: 1. The size of spleen were reduced after MTX treatment more on dose day by day. 2. The periarterial lymphatic sheath (PALS) and germinal center were also reduced after MTX treatment. 3. The lymphocytes of white pulp showed nuclear pyknotic changes and many nuclear debris were found after MTX treatment. 4. The brown hemosiderin pigments were increased markedly in the red pulp of MTX treated groups. 5. Marked proliferation of fibrous trabeculae were found in the red pulp of MTX treated mice.

사람 말초혈액 림프구에서 Bromodeoxyuridine에 의해 유도되는 Fraglie Sites

박경란,김원식 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

Chromosomal abnormalities including fragile sites induced by 50㎍/ml and 100㎍/ml bromodeoxyuridine (BrdU) was studied in lymphocytes cultures of six healthy individuals. A significant decrease in mitotic indexes in respect to control cultures was observed with both BrdU concentrations used. The cells showing chromosome aberrations and the total number of cytogenic alterations were significantly increased both BrdU treated cultures with respect to control cultures. Damaged cells showing chromosome aberrations and total number of chromosomal aberrations were increased in BrdU, 100㎍/ml treated groups compared to BrdU 50㎍/ml treated groups. The most frequent type of chromosomal aberration is a gap. A site showing a gap or break was defind as fragile if it appeared in 1% of cells analyzed and in at least three of six individuals studied with the same culture treatment. Using these criteria, fragile sites 4q31, 5q31, 6p22, 6q13, 9q21, and 15q24 were induced in different proportions by different concentration of BrdU.

사람 말초혈액 림프구에서 FudR에 의해 유도되는 Fragile Sites

박경란,신웅기,박성국,김원식 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.1

Chromosomal abnormalities including fragile sites induced by 10 ㎍/ml and 20 ㎍/ml fluorodeoxyuridine (FudR) were studied in lymphocytes cultures of six healthy individuals. A significant decrease in mitotic indexes in respect to control cultures was observed with two FudR concentrations used. The cells showing chromosome aberrations and the total number of cytogenetic alterations were significantly increased both FudR treated cultures with respect to control cultures. Damaged cells showing chromosome aberrations and total number of chromosomal aberrations were not significantly increased in FudR 20 ㎍/ml treated groups compared to FudR lO㎍/ml treated groups. The most frequent type of chromosomal aberration is a gap. A site showing a gap or break was defind as fragile if it appeated in 1% of cells analyzed and in at least three of six individuals studied with the same culture tre atment. Using these criteria, fragile sites 3p14, 6p25, 3q32, 1Oq22, 12q21 and 13q21 were induced in different proportions by different concentration of FudR.

간질환환자의 말초임파구 염색체에서 Fragile Site의 의의

박경란,김수일,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.1

To understand chromosomal instability in hepatitis B virus related disease and significance of fragile sites in hepatoma, lymphocytes of peripheral blood from patients of hepatitis, liver cirrhosis and hepatoma were cultured. Fragile sites of chromosomes were counted by chromosome group and the results were as follows. 1. The frequencies of fragile sites were significantly increased in hepatitis, liver cirrhosis and hepatoma compared with control group. 2. The frequency of fragile sites by chromosome group were appeared in chromosome C, A, B and E group in turn on control group, chromosome C, A and B in turn on hepatitis and liver cirrhosis, and chromosome C, B and A in turn on hepatoma respectively. 3. The frequency of fragile sites was significantly increased in liver cirrhosis with HBsAg( + ) in comparison to HBsAg(-) liver cirrhosis. However no differences of frequency were founded in hepatoma by presence of HBsAg. 4. The common specific fragile sites of chromosome were appeared in chromosome 2q13, 5q34, 6p22 and 11q13, It is presumed that chromosomal instability by hepatitis B virus may induce hepatocellular carcinoma, and the common fragile sites found in chromosome 2q13, 5q34, 6p22 and 11q13 would be correlated with sites of oncogenes. However, more study needs to he done in correlation between fragile sites and oncogenes.

제 1형 신경섬유종증 환자에서 발생한 위장관 간질종양 1예

원경준,이준,변유미,조민근,한경택,김원,홍란,임성철,김경종,김영대,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Gastrointestinal stromal tumor (GIST) is the most common non-epithelial neoplasm arising in the gastrointestinal tract, but this tumor is rarely seen in association with type l neurofibromatosis (NFl) Generally, the majority of GISTs are isolated neoplasm and they are sporadically found in the stomach, but the GISTs in NFl patients are usually multiple and usually found in the small intestine. We report a case of multiple GISTs in the jejunum of a 63-year-old woman diagnosed as NFl accompanied by complication of gastrointestinal bleeding, In this case, Patient had multiple cafe-au lait spots and neurofibroma on skin and had freckling on axilla and groin, and then, we made a diagnosis of NFl. Gastrointestinal bleeding is controlled by resection of multiple GISTs. Generally, only 3-5% of all gastrointestinal bleeding comes from the small bowel. Causes of small intestinal bleeding are angiodysplasia, neoplasm, NSAIDs induced ulcer etc. but, If patients have NFl accompanied by complication of gastrointestinal bleeding, GISTs on small bowel must be considered.

교원의 교원능력개발평가 결과, 인구학적 특성과 전문성 인식의 관계

박지혜,이경란,문은주 이화여자대학교 교육과학연구소 2015 교육과학연구 Vol.46 No.4

This study aimed to provide useful information by investigating the relationships between the results of teacher evaluation for professional development, teachers’ demographic characteristics, and their perception of expertise. Among those who participated in the survey asking teachers’ perception of the 2014 teacher evaluation for professional development, the data from 1,622 teachers in the upper and lower limits of one standard deviation of teacher peer evaluation and student satisfaction were used. Study findings were as follows. First, statistically significant and positive correlations existed between teacher evaluation results and teachers’perception of expertise. Second, female teachers, those whose career spanned 5-15 years, elementary teachers perceived their expertise higher than those of male teachers, those whose careers did not span 5-15 years, and middle and high school teachers. Third, mean scores of expertise perception of teachers who had high scores on peer evaluation and student satisfaction were higher than those of other groups. Last, providing information to parents and appropriate evaluation were the key factors distinguishing those who had high scores on teacher evaluation and student satisfaction from those had high scores on teacher evaluation and low scores on student satisfaction. In addition, providing information to parents was the key factor differentiating those who had high scores on teacher evaluation and student satisfaction from those who had low scores on both teacher evaluation and student satisfaction. The significance of these study results was discussed along with limitations and suggestions for future research studies.

總輪膽管結紮術후 小腸 Paneth細胞의 變化에 關한 硏究

金容佑,朴景蘭,金源式 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

For the study on the changes of Paneth cells and paneth cell granules in small intestine of rats(Sprague Dawely, male, b.w. 200gm±10gm) after common bile duct ligation, the rats were sacrificed at 5, 15, 30 and 45 days after operation, and removed duodeum, jejunum and ileum. Tissues were fixed with 10% neutra formalin solution for over 24 hours. After PAS reaction, Paneth cells were counted within well defined intestinal crypts and the Paneth granules were classified as type I, Ⅱ and Ⅲ according to the granular size and distribution within the cytoplasm. The results were as follows; 1. Paneth cell indices of duodenum and jejunum were decreased significantly from 15 days after common bile duct ligation to 45 days. 2. Paneth granular in dices of duodenum and jejunum were decreased significantly from 15 days after common bile duct ligation to 45 days. 3. Goblet cells were increased in the base of crypts of duodenum and jejunum after common bile duct ligation from 15 days to 45 days.

한국주부의 제례행례에 관한 조사연구

안숙자,박경란 중앙대학교 생활과학연구소 2001 생활과학논집 Vol.14 No.-

This study is to divide the sacrificed area into a city and a country, to survey the influence of the its local characteristics on housekeepers' sacrificial behavior, to investigate how general environmental factors influence on housekeepers' sacrificial behaviors living in a city and a country and finally, to make it a basic data for succeeding to traditional culture through understanding a modern meaning of sacrifice. 1.Basic sacrificial behaviors of housekeepers was not showing meaningful difference between a city and a country. Most of housekeepers were showing the affirmative attitudes. 2.Basic sacrificial behaviors was much higher, as the age was older(P<.01), the religious of her husband's family was Confucianism, Buddhism and Atheist(P<.001), housekeeper's religion was Confucianism, Buddhism (P<.001), her academic carrier was lower(P<.001) and her job was more plain (P<.001). 3.The correlations of basic sacrificial behaviors each other have a statical correlation on the whole. 4.Considering between the basic sacrificial behaviors and general variation of the object of the study, as the age was older, her academic carrier was lower and her job was more plain, its behaviors was high. 5.A country was more traditional than a city in 'time of a memorial sacrifice held on the anniversary of death'(P<.01), 'range of the sacrificial rite'(P<.01),'range of ancestor-memorial rites'(P<.05), 'use or not use of an ancestral tablet made of paper and written prayer'(P<.05). But, in 'sacrificial ceremony', 'leader in sacrifice', 'method of an ancestral tablet made of paper and written prayer', 'managing method of an ancestral tablet and written prayer', 'method to prepare the foods for the sacrifice', a city and a country both were pursuing traditional tendency. 6.The following are the sacrificial behaviors resulted from general factors. 1)'Sacrificial ceremony' was performed by more 'traditional methods' as the religious of her husband's family or housekeeper was Confucianism, Buddhism and Atheist(P<.001), her academic carrier was lower(P<.01) and her job was more plain(P<.001). 2) Regarding 'the time of memorial sacrifice for the anniversary of death', as the age was older(P<.001), the religious of housekeeper or her husband's family was Confucianism, Buddhism and Atheist(P<.001), order in family of her husband was higher(P<.05), her academic carrier was lower(P<.001) and her job was more plain(P<.001), they were performed it 'night before the date of ancestor's death' 3)'leader of sacrifice' was mainly the eldest grandson by the eldest son, as the religious of her husband's family or housekeeper was Confucianism and Buddhism(P<.001, P<.01), order in family of her husband was higher(P<.001) and her job was more plain(P<.001). 4)'Range of memorial sacrifice for the anniversary of death' was upper the 3rd generation, the 4th generation or more, as the religious of her husband's family or housekeeper was Confucianism, Buddhism and Atheist(P<.001), order in family of her husband was higher(P<.05), her academic carrier was lower(P<.01), her job was more plain(P<.001) and their income was higher(P<.05). 5)'Range of ancestor-memorial rites' was upper the 3rd generation, the 4th generation or more, as the religious of her husband's family or housekeeper was Confucianism, Buddhism and Atheist(P<.001), order in family of her husband was higher(P<.05) and her job was more plain (P<.001). 6) Regarding the 'use or not use of an ancestral tablet made of paper and written prayer', they were using only 'the ancestral tablet without written paper', as the age was younger(P<.01) and the religious of her husband's family or housekeeper was Confucianism, Buddhism and Atheist(P<.001). 7) Regarding 'using method of an ancestral tablet and written prayer', 'Chinese letter' was used traditionally, as the religious of her husband's family or housekeeper was Confucianism, Buddhism and Atheist (P<.001), her academic carrier was lower(P<.05), her job was more plain(P<.05) and their income was lower(P<.05). 8) Regarding 'managing method of an ancestral tablet and written prayer', they were using 'burning method', as the religious of her husband's family or housekeeper was Confucianism, Buddhism and Atheist (P<.001) and her job was more plain(P<.05). 9) Regarding 'method to prepare sacrificial foods' they were following up 'the traditional method of the family of her husband', as the religious of her husband's family or housekeeper was Confucianism, Buddhism and Atheist(P<.001), her academic carrier was lower(P<.001), her job was more plain(P<.001). Considering the above results, we can find that basic sacrificial behaviors was not any difference between a city and a country meanwhile 'time of a memorial sacrifice for the anniversary of death', 'range of the memorial sacrifice for the anniversary of death', 'range of ancestor- memorial rites', 'use or not use of an ancestral tablet made of paper and written prayer' was difference between a city and a country and all sacrificial behaviors was generally different according to age, religious of her husband's family or housekeeper, academic carrier. job, order in the family of the husband and income of the family.