생쥐에 있어서 單一害球가 分離된 受精卵의 移植에 의한 새끼生産에 관한 硏究

崔有林,全益秀,金宣宜,崔光洙,朴修奉,辛明宰 慶北大學校農業科學技術硏究所 1991 慶北大農學誌 Vol.9 No.-

本 연구는 생쥐의 4細胞期胚에서 하나의 割球를 뽑아내는 새로운 割球分離技術인 biopsy에 대한 효율성과 分離된 受精卵과 分離한 割球의 생존성 및 새끼생산율을 검토한 結果이다. 그 結果를 要約하면 다음과 같다. 1. 4細胞期胚에서 分離한 單一割球와 4細胞期胚를 M2 배양액에 배양한 結果, 單一割球는 82.6%가 영양배엽을 형성한 割球로 發生하였고 4細胞期胚로 89.5%가 胚盤胞期胚로 發生하였다. 2. 4細胞期胚를 biopsy하여 하나의 割球가 分離된 受精卵과 대조구인 4細胞期胚를 M2배양액에서 배양한 결과 각각 83.3%와 90.4%가 胚盤細胞胚로 發生하였다. 3. Biopsy하여 4細胞期胚에서 分離한 單一割球와 대조구인 4細胞期胚에서 4개의 割球로 분리한 單一割球를 M2 배양액에서 배양한 결과 각각 80.8%와 83.3%가 영양배엽을 형성한 割球로 發生하였다. 4. 4細胞期胚에서 하나의 割球를 뽑아낸 受精卵과 대조구인 4細胞期胚를 수란생쥐에 이식한 결과 각각 36.0%와 48.6%의 새끼쥐 생산率을 얻었다. The study was carried out to investigate the viability and the offspring production rate of single blastomeres and biopsied 4-cell mouse embryos and, also to examine the efficiency of biopsy. The results obtained are summerised as follows : 1. The separated blastomeres from 4-cell embryos and intact 4-cell embryos which were cultured in Medium 2 were developed to trophoblastic vesicle and blastocyst embryo by 82.6% and 89.5%, respectively. 2. The biopsied embryos from 4-cell embryos and intact 4-cell embryos which cultured in Medium 2 were developed to blastocyst embryo by 83.3% and 90.4%, respectively. 3. The biospied blastomeres and the separated blastomeres from 4-cell embryos which were cultured in Medium 2 were developed to trophoblastic vesicle by 80.8% and 83.3%, respectively. 4. The biopsied embryos from 4-cell embryos and intact 4-cell embryos were transferred to recipients, and the offspring rate was 36% and 48.6%, respectively.

최근 10년간 원광의대병원에서 시행한 소아 심장혈관 수술의 임상적 고찰

윤향석,정수미,최두영,오광수,오연균,김종덕,양현웅,이삼윤,김형곤,최종범,최순호,노병석 圓光大學校 醫科學硏究所 1995 圓光醫科學 Vol.11 No.2

We reviewed 10 years experiences of the clinical aspects and early postoperative results in 544 pediatric patients(age less than 16 years old). Among them. 529 cases had the congenital heart diseases, and 15 had the acquired diseases. Open heart surgery was performed in 413 patients. 115 cases were treated with non-open heart surgery. 16 with interventional catheterization. Annual increase of the cardiac patients was not significant in recent 10 years, except slight increase in 1994. The mean age of the study patients had been evidently changed to younger year by year. Total mortality rate of the open heart surgery was 6.4%. The cyanotic congenital heart diseases were high in early postoperative mortality by 25.7%, whereas 4% in acyanotic group. The age group less than one month was most highest in surgical mortality(66.7%). There were no deaths in patients with patent ductus arteriosus(103 cases), atrial septal defect(83 cases) or pulmonary stenosis(17 cases). Among 220 patients with ventricular septal defects(VSD). 10(4.5%) were died early postoperatively. In cyanotic group, the patients with pulmonary atresia with ventricular septal defects(PAVSD), transposition of the great arteries(TGA), interrupted aortic arch(IAA), complex cardiac anomalies with isomerism seemed to be most susceptible to an early death.

국산 Lidocaine 알칼리화를 위한 Bicarbonate 혼합시 침전유발 최소량에 대한 고찰

이선호,김현수,곽인숙,안원식,최관호,김광민,손민제 대한마취과학회 2000 Korean Journal of Anesthesiology Vol.39 No.5

Background : When local anesthetics for regional anesthesia is used, usually small amounts of bicar-bonate are added for rapid onset. This addition gives the mixed solution a more alkaline pH. The following result is an increased unionized form of the local anesthetic and rapid penetration of the drug into tiissue. Unfortunately, no data about adequate mixing volumes of domestic lidocaine and bicarbonate is available. Methods : We examined six mixing pairs of two kinds of 2% lidocaines and three kinds of 8.4% bicarbonates for minimum volumes of bicarbonate to cause a precipitation of 2% 20 ml lidocaine. Results : The mean volumes of bicarbonate to cause precipitation were 1.54 ml for Kwang-Myung lidocaine with Kwang-Myung bicarbonate, 2.90 ml for Kwang-Myung lidocaine with Dae-Won bicarbonate, 2.73 ml for Kwang-Myung lidocaine with Je-Il bicarbonate, 0.97 ml for Je-Il lidocaine with Kwang-Myung bicarbonate, 1.26ml for Je-Il lidocaine with Dae-Won bicarbonate and 1.39 ml for Je-Il lidocaine with Je-Il bicarbonate. Conclusions : We conclude that the Kwang-Myung lidocaine and the Je-Il lidocaine could cause precipitation when mixing with a smaller bicarbonate volume than foreign textbook recommended. (Korean J Anesthesiol 2000; 39: 726-729)

원자력 사고후 쌀알과 배추내 ^90Sr 및 ^137Cs 농도 예측

최용호,임광묵,황원태,이한수,이창우 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.3

원자력 사고후 시간경과에 따른 작물체내 핵종 농도를 보다 현실적으로 예측할 수 있는 방법을 수립하고 단위 건침적에 대하여 백미와 배추내 ^90Sr 및 ^137Cs의 50 년간 농도를 예측하였다. 비생육기 사고의 경우 농도는 두 핵종 모두 시간경과에 따라 서서히 감소하였고 50년 내내 ^90Sr이 ^137Cs보다 높았다. 생육기 사고의 경우 처음 1년간 농도는 비생육기 사고에 비해 대체로 ^90Sr은 최고 30 배, ^137Cs은 최고 1,000 배 정도까지 높았다. 50 년간의 누적농도는 백미에서는 비 생육기 사고의 경우 ^90Sr이, 생육기 사고의 경우 ^137Cs이 더 높았으나 배추에서는 어느 경우에나 ^90Sr이 더 높았다. 생육기 사고시 50년간의 누적농도에 대한 지배적 경로는 대체로 ^90Sr의 경우 뿌리흡수, ^137Cs의 경우 작물체 직접오염이었다. 재부유의 영향은 무시할 수 있을 정도였다. 예측 결과에 입각하여 사고 조건별로 대책의 방향을 제시하였다. A method of more realistically predicting radionuclide concentrations in crop plants varying with time after a nuclear accident was established to estimate 50 years' concentrations of ^90Sr and ^137Cs in polished rice seeds and Chinese cabbage for unit dry deposition. After non-growing season accidents, concentrations of both nuclides decreased gradually with time and ^90Sr concentrations were higher than those of ^137Cs throughout the whole period. Radionuclide concentrations in the 1st year after growing season accidents were on the whole higher than those after non-growing season accidents by factors of up to 30 for ^90Sr and up to 1,000 for ^137Cs. In polished rice seeds, the 50 years-integrated concentration was higher for ^90Sr than for ^137Cs after non-growing season accidents, whereas the opposite was true after growing season accidents. In Chinese cabbage, however, it was higher for ^90Sr than for ^137Cs after both types of the accident. Generally speaking, the dominant pathway for the integrated concentration after the growing season accident was root uptake for ^90Sr and direct plant contamination for ^137Cs. The effect of resuspension was negligible. Based on the predicted results, the direction of planning countermeasures was suggested for various accident conditions.

제2형 당뇨병 환자에서의 지속적 피하 인슐린 주입 치료시 관해

최의광,최수봉,박선민 건국대학교 의과학연구소 2001 건국의과학학술지 Vol.11 No.-

Background: It is well known that chronic hyperglycemia can deteriorate pancreatic beta cell function and insulin sensitivity in animal and human studies, and the normalization of blood glucose can reverse them. Our purpose of the study was to investigate the remission pattern after treating patients with continuous subcutaneous insulin infusion (CSII) therapy, and compare the clinical characteristics of remitted and non-remitted patients in a retrospective way. Methods: We selected 91 type 2 diabetic patients who had been admitted to KonKuk University hospital from January 1993 to August 1993. After hospitalization, all subjects were treated with insulin pump (Sooil, Seoul, Korea) for intensive blood glucose control until remission occurred. Remission is defined as long-term good glycemic control without any medication. After fifteen to seventeen months of CSII therapy, the induction of remission was examined. Serum c-peptide levels, hemoglobin A1c, and serum lipid profiles were measured after hospitalization and before discharge. Results: Overall 34.4% of all subjects had remission after 53.6±38.9 days of CSII therapy and remission lasted for average 13.6 ± 8.9 months during the study periods. Some clinical characteristics of the patients prior to CSII therapy influenced the remission occurrence. The remission was more frequently induced when patients started CSII therapy with insulin pump in younger age, higher body mass index, shorter diabetic duration, lower post-prandial blood glucose levels, higher post-pandial serum c-peptide levels, and less chronic diabetic complications. Conculsion: These findings suggest that long-term CSII therapy can induce remission in a significant proportion of mild type 2 diabetic patients. Thus, it is desirable that the intensive insulin treatment by CSII is considered as not the last treatment., but an initial management of mild type 2 diabetic patients.

T 형강을 사용한 합성골조 보-기둥 접합부의 비선형 해석모델에 관한 연구

최광호,김영수 남서울대학교 2005 남서울대학교 논문집 Vol.11 No.1

A series of studies have been performed to develop the hybrid beam-column connection system with structural tee, and the experimental research was conducted to verify the fabrication efficiency and the structural performance in the first step. This research is aimed at the verification of the values measured from the previous tests by FEM analysis based on material nonlinearity. Three types of analytical model reflected in the joint behavior have been proposed and finite element analysis has been conducted with the factors simulating the experimental conditions. In the analysis, von Mises yielding condition with bilinear strain hardening model was adapted with some subsidiary conditions, such as the contact conditions and the multi-point constraints. The validity of the analytical models has been scrutinized by comparing the values obtained from the elastic-plastic analysis with the previously conducted test results. Following the observation of test and analysis, the proposed connection system has sufficient rotational stiffness against the increase of load so that the plastic hinge and the failure has been occured at the wide flange beam, by which proposed connection has been proved to take the ductility for structural safety.

고리 및 영광 논토양에 대한 ^137Cs의 모의 사고 침적시 토양-벼 전이계수

최용호,임광묵,박효국,최희주,이한수 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.4

고리 및 영광 원전 반경 5km 내 11곳의 논토양을 원상대로 재배상자에 채취하여 실험 온실로 운반한 후 벼의 이식 전과 생육중에 ^137Cs을 담수의 표면에 처리하였다. 이식전 처리시 ^137Cs의 토양-작물체 전이계수(m²kg^-1-dry plant)는 토양에 따라 8배 정도의 변이를 보였고 평균은 지역간 차이가 거의 없이 쌀알의 경우 1.1 x 10^-4 , 볏짚의 경우 2.7 x 10^-4 이었다. 분얼초기 처리와 수잉기 처리시 전이계수는 이식전 처리에 비해 대체로 2∼3 배 및 15∼30 배 정도 높았다. 토양 유기물 함량과 전이계수 간에는 정의 상관이 있었다. 본 실험결과에 입각하여 우리나라 논에 대한 일회성 사고침적시 적용할 수 있는 ^137Cs 전이계수의 대표치를 침적시기별로 제안하였다. Undisturbed soil blocks were taken into culture boxes from 11 paddy fields within 5 km radii of Kori and Yonggwang NPPs and carried to a greenhouse where ^137Cs was applied to the surface of standing water before rice transplanting and during its growth. Transfer factors (m²kg^-1-dry plant) of ^137Cs applied before transplanting varied with soils by a factor of about 8. The mean was 1.1 x 10^-4 for hulled rice seeds and 2.7 x 10^-4 for straw without significant regional differences. The ^137Cs applications at the early tillering stage and booting stage resulted in, on the whole, 2∼3 and 15∼30 times, respectively, higher transfer factors than the application before transplanting. A positive correlation was found between soil organic matter and transfer factor. Generic values of transfer factors were proposed for an acute accidental deposition of ^137Cs onto Korean paddy fields in different times of the year.

Bowen양 구진증의 조직학적 소견과 랑게르한스 세포수의 변화에 관한 연구

이광길,최순임 대한병리학회 1990 Journal of Pathology and Translational Medicine Vol.24 No.3

재생골재를 사용한 콘크리트의 특성에 관한 실험적 연구

최광윤,배수환,정재동,김한철,최영화 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

The purpose of this experiment is an experimental study on recycling of waste concrete and suggest a possibility of the Recycled Aggregate Concrete after establishing some kinds of experimental properties of concrete. Therefore, this study compared and investigated properties of natural aggregate concrete and recycled aggregate concrete by replacing the ratio of recycled aggregates under the absorptions, compressive strengths and chemical resistances. As the result, the compressive strength and the chemical resistance of Recycled Aggregate Concrete is lower than the natural aggregate concrete. If was concluded that the properties of the recycled aggregates and lower than natural aggregates.

소수성 촉매를 이용한 수소동위원소 교환 반응 측정을 위한 순환 반응기

최희주,이한수,안도희,강희석,김광락 한국공업화학회 2002 응용화학 Vol.6 No.2

The catalytic rate constants of hydrophobic catalysts developed for the removal of tritium mainly generated in the pressurized heavy water reactors should be measured. A recycle reactor was designed and built for characterzing the long-term behavior of the catalyst performance at the various temperatures and gas velocities.The catalytic rate constants were measured at the apparent gas velocity of 0.5 m/s and the temperature of 60℃. The initial catalytic constant was 8.44×10^-4mol/g.sec. This value is much greater than that considered in the design of Wolsong Tritium Removal Facility.