Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

내시경적 점막절제술로 치험한 식도 과립상 세포종 1례

강혁주,김성욱,최석진,이중현,장재식,서영범,윤병구,박건욱,김성자,김용섭,강승완,이구,양창헌,이창우,김욱년,이광헌,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

과립상 세포종은 Schwann 세포 기원으로 생각되며 인체에 비교적 드물게 발생한다. 과립상 세포종은 전신 어느 곳에서나 발견될 수 있으나 주로 혀, 구강, 피부 혹은 유방 등에서 호발하며 드물게 위장관에서 발견된다. 위장관에서는 식도에서 가장 호발하며 다음으로 위, 대장 순이다. 과립상 세포종은 대부분, 특히 위장관에서는 양성이며 소수의 악성 병변이 보고되었다. 이러한 이유와 함께 수술 전의 진단이 어렵기 때문에 과립상 세포종에 대한 근본적인 치료는 현재까지 외과적 절제술이다. 최근에 시도되는 치료방법들로는 내시경적 레이저 치료, 용종절제술, 내시경적 점막 절제술 등이 있다. 저자들은 상부 소화관 내시경검사를 시행하여 식도 과립상 세포종을 진단하고 내시경적 점막 절제술을 시행하여 합병증 없이 퇴원하여 현재 재발없이 경과 관찰중인 1례를 경험하였기에 보고하는 바이다. Granular cell tumors, which occur infrequently, are probably of Schwann cell origin. They can occur almost anywhere in the body but usually affect the tongue, oral cavity, skin, or breasts and are rarely found in the gastrointestinal tracts. The esophagus is the most frequent gastrointestinal site, followed by the stomach and the colon. Granular cell tumors are generally benign, especially in the gastrointestinal tract, some malignant lesions have been reported. For this reason, and also because preoperative diagnosis is difficult, the standard treatment for granular cell tumor has until now been surgical excision. In recent years, other therapeutic methods is endoscopic laser therapy (ELT), polypectiomy, endoscopic mucosal resection (EMR). We report a case of esophageal granular cell tumor which was diagnosed by an endoscopy and managed using an endoscopic mucosal resection without complication.

Kidney Toxicity Induced by 13 Weeks Exposure to the Fruiting Body of Paecilomyces sinclairii in Rats

Jeong, Mi-Hye,Kim, Young-Won,Min, Jeong-Ran,Kwon, Min,Han, Beom-Suk,Kim, Jeong-Gyu,Jeong, Sang-Hee Korean Society of ToxicologyKorea Environmental Mu 2012 Toxicological Research Vol.28 No.3

Paecilomyces sinclairiis (PS) is known as a functional food or human health supplement. However concerns have been raised about its kidney toxicity. This study was performed to investigate the kidney toxicity of PS by 13 week-oral administration to rats. Blood urea nitrogen (BUN), serum creatinine, and kidney damage biomarkers including beta-2-microglobulin (${\beta}2m$), glutathione S-transferase alpha (GST-${\alpha}$), kidney injury molecule 1 (KIM-1), tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), vascular endothelial growth factor (VEGF), calbindin, clusterin, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL) and osteopontin were measured during or after the treatment of PS. BUN, creatinine and kidney damage biomarkers in serum were not changed by PS. However, kidney cell karyomegaly and tubular hypertrophy were observed dose-dependently with higher severity in males. KIM-1, TIMP-1 and osteopontin in kidney and urine were increased dose dependently in male or at the highest dose in female rats. Increased urinary osteopontin by PS was not recovered at 2 weeks of post-exposure in both genders. Cystatin C in kidney was decreased at all treatment groups but inversely increased in urine. The changes in kidney damage biomarkers were more remarkable in male than female rats. These data indicate that the PS may provoke renal cell damage and glomerular filtration dysfunction in rats with histopathological lesions and change of kidney damage biomarkers in kidney or urine. Kidney and urinary KIM-1 and cystatin C were the most marked indicators, while kidney weight, BUN and creatinine and kidney damage biomarkers in serum were not influenced.

Kidney Toxicity Induced by 13 Weeks Exposure to the Fruiting Body of Paecilomyces sinclairii in Rats

Mihye Jeong,Young-Won Kim,Jeong-Ran Min,Min Kwon,Beom-Suk Han,Jeong-Gyu Kim,Sang-Hee Jeong 한국독성학회 2012 Toxicological Research Vol.28 No.3

Paecilomyces sinclairiis (PS) is known as a functional food or human health supplement. However concerns have been raised about its kidney toxicity. This study was performed to investigate the kidney toxicity of PS by 13 week-oral administration to rats. Blood urea nitrogen (BUN), serum creatinine, and kidney damage biomarkers including beta-2-microglobulin (β2m), glutathione S-transferase alpha (GST-α), kidney injury molecule 1 (KIM-1), tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), vascular endothelial growth factor (VEGF), calbindin, clusterin, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL) and osteopontin were measured during or after the treatment of PS. BUN, creatinine and kidney damage biomarkers in serum were not changed by PS. However, kidney cell karyomegaly and tubular hypertrophy were observed dose-dependently with higher severity in males. KIM-1, TIMP-1 and osteopontin in kidney and urine were increased dose dependently in male or at the highest dose in female rats. Increased urinary osteopontin by PS was not recovered at 2 weeks of post-exposure in both genders. Cystatin C in kidney was decreased at all treatment groups but inversely increased in urine. The changes in kidney damage biomarkers were more remarkable in male than female rats. These data indicate that the PS may provoke renal cell damage and glomerular filtration dysfunction in rats with histopathological lesions and change of kidney damage biomarkers in kidney or urine. Kidney and urinary KIM-1 and cystatin C were the most marked indicators, while kidney weight, BUN and creatinine and kidney damage biomarkers in serum were not influenced.

벼 재배양식에 따른 규산질 비료 추비 사용 효과

金正鎬,吳成煥,李哲遠,宋凡憲,孫錫龍 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.1

본 시험은 벼 도복을 경감시키기 위한 재배대책을 강구하기 위하여 광안벼를 공시하고, 이앙재배와 담수직파재배에서 규산질 비료를 사용함에 따른 도복경감효과를 조사하였던 바 그 결과를 요약하면 다음과 같다. 1. 간장은 담수표면직파재배에서 규산시용구가 무처리보다 3.1cm 감소하여 3.7%가 단축되었으며, 기계이앙재배에서는 규산시용구가 무처리보다 5cm 감소하여 5.5% 단축되었다. 2. 하위절간장은 담수표면직파재배에서 규산시용구가 무처리보다 3.1cm 감소하여 36.9% 단축되었으며, 기계이앙재배에서는 규산시용구가 무처리보다 1.2cm 감소하여 11% 단축되었다. 3. 좌절중은 담수표면직파에서 규산시용구가 73g이 증가하여 35.2%의 증가율을 보였고, 기계이앙재배에서는 규산시용구가 37.3g 증가하여 9.6%의 증가율을 보였다. 4. 수량은 담수표면직파재배에서 규산시용구가 무처리보다 30kg이 많아 6.4%의 증수효과를 나타내었고, 기계이앙재배에서는 규산시용구가 무처리보다50kg이 많아 9%의 증수효과를 나타내었다. This study was carried out to examine the responses of growth and lodging of rice as affected by both two cropping practices, transplanting and direct seeding in waterlogging, and different application amounts of silicate fertilizers. The Kwangan cultivar of Japonica type was used, the growths including culm and internode lengths and grain yield were investigated in major growth stages and harvest time. The culm length in waterlogging direct seeding was reduced in treatments of silicate fertilizers, about 3.7%, compared to that in the control and it was reduced to 5.5% in the transplanting cultivation. The breaking strength of lower internodes of rice were increased to 35.2% and 9.6% by applying of silicate fertilizers in the water direct seeding and in the transplanting cultivations, respectively. The rice yields were increased with application of silicate fertilizers in both water direct seeding and transplantingcultivations compared to those of the control, about 6.4% and 9.0%, respectively.

제주도 연안 정치망 조업시스템 개발에 관한 연구 : 낙망에 의한 조업시스템 생력화 Studies for the Man Power Saving of Fishing System by Pound net

김병엽,김석종,이창헌,김종범,서두옥 濟州大學校 海洋硏究所 2002 해양과환경연구소 연구논문집 Vol.26 No.-

제주도 연안 정치망의 조업시스템 생력화와 실용화를 목적으로 어군의 도피를 최대한 방지하고 양망작업이 용이하도록 개량 설계된 낙망어구를 제주도 북제주군 한경면 금등정치망 어장에 부설하고 실용화 및 조업시스템 생력화에 대하여 현장실험을 실시하였는데 그 결과를 요약하면 다음과 같다. 1. 낙망어구규모에 적합한 조업선을 기계화하여 1척의 어선으로 조업이 가능하였다. 2. 조업어선 선수미부 양망현에 양망기인 캡스턴을 설치하므로 조업인원 2∼3명으로도 양망작업이 가능하였다. 3. 망갈이 작업시 양망한 그물을 선상에서 적재하고 육상에서 양륙할 때 파워블록을 사용함으로써 작업효율을 높일 수 있었다. In order to obtain the fundamental data for the man power saving and the practical use of fishing system for a set net in the coast of Jeju island, a pound net designed for making hauling operation easier and preventing a school of fish from escaping was set up at a fishing ground in Geumdeung and a field experiment was carried out. The fishing operation was feasible with one boat mechanized for the pound net and the hauling operation was not made possible with 2-3 persons till a net haular, that is, a capstan was set up on the hauling side of the boat. In addition, a power block enhanced the efficiency of operation for hauling up the net on board or hauling down on land.

Aluminum응집제들에 의한 금속이온들의 응집에 관한 연구

김미향,최범석 慶熙大學校 材料科學技術硏究所 1999 材料科學技術硏究論集 Vol.12 No.-

Coagulation efficiencies of inorganic ions by aluminum based coagulants including alum, PAC, PACS were studied. Pb, As, Cu, Hg ions shows good coagulation efficiences with all the coagulants. Residual amount of Hg is also decreased by the precipitation as a hydroxide. The maximum coaulation efficiency is observed at the neutral pH and the efficiency is decreased with lowering pH, except Se. The coagulation efficiencis are decresed with an order of PACS, PAC, and alum. Cr, Cd shows lower coagulation efficiencies and Ni and Mn are not coagulated with all the coagulants.

Lutembacher 증후군 환자 증례 보고

김성범,서정주,곽철훈,김상민,이보라,민선경,황은구,김용인,조욱현,최석구 인제대학교 2008 仁濟醫學 Vol.29 No.-

Lutembacher's syndrome is rare combination of mitral stenosis (MS) and atrial septal defect (ASD). The hemodynamic interplay between the MS and ASD leads to wide variation of clinical presentation. Here we describe a 43-year-old female with Lutembacher's syndrome and tricuspid regurgitation with pulmonary hypertension, who underwent direct closure of ASD and tricuspid valvuloplasty successfully. We also reviewed other literatures in an effort to increase awareness of this condition.

벼 재배방법과 규산질 비료 시용이 생육 및 도복에 미치는 영향

金正鎬,吳成煥,李哲遠,宋凡憲,孫錫龍 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-

본 시험은 벼 도목을 경감시키기 위한 재배대책을 강구하기 위하여 광안벼를 공시하고, 이앙재배와 담수직파재배에서 규산질 비료를 시용함에 따른 도복경감효과를 조사하였던 바 그 결과를 요약하면 다음과 같다 1 간장은 담순표면직파재배에선 규산시용구가 무처리보다 31cm 감소하여 37%가 단축되었으며, 기계이앙재배에서는 규산시용구가 무처리보다 5cm 감소하여 55% 단축되었다 2 하위절간장은 담수표면직파재배에서 규산시용구가 무처리보다 3.1cm 감소하여 36 9% 단축되었으며, 기계이앙재배에서는 규산시용구가 무처리보다12cm 감소하여 11% 단축되었다 3 좌절중은 담수표면직파에서 규산시용구가 73g이 증가하여 35 2%의 증가율을 보였고, 기계이앙재배에서는 규산시용구가 373g 증가하여 9.6%의 증가율을 보였다 4 수량은 담수표면직파재배에서 규산시용구가 무처리보다 30kg이 많아 64%의 증수효과를 나타내었고, 기계이앙재배에서는 규산시용구가 무처리보다50kg이 많아 9%의 증수효과를 나타내었다 This study was carried out to examine the responses of growth and lodging of rice as affected by both transplanting and direct seeding in waterlogging, which was differently applicated amounts of silicate fertlizers The Kwangan cultivar of Japonica type was used The growths including culm and internode lengths and grain yield were investigated in major growth stages and harvest time The culm length in waterlogging direct seeding was reduced in treatments of silicate fertilizers, about 37%, compared to that in the contro1 and it was reduced to 5.5% in the transplanting cultivation The breaking strength of lower internodes of rice were increased to 35.2% and 96% by applying of silicate fertilizers in the water direct seeding and in the transplanting cultivations, respectively The rice yields were increased with application of silicate fertilizers in both water direct seeding and transplanting cultivations compared to those of the control, about 64% and 90%, respectively.

벼 담수직파 재배시 생장조절제 처리가 생육 및 도복에 미치는 영향

金正鎬,吳成煥,李哲遠,宋凡憲,孫錫龍 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-

본 시험은 벼 도복을 경감시키기 위한 재배대책을 강구하기 위하여 추청벼를 공시하고, 담수직파재배에서 생장조절제를 처리함에 따른 도복경감효과를 조사하였던 바 그 결과를 요약하면 다음과 같다. 1 간장은 무처리구보다 IBP+Inabenfide구가 186cm 단축되어 가장 큰 감소효과를 보였다 2 하위절간장 역시 무처리구보다 IBP+Inabenfide구가 3-4절간 35cm, 4-5절간은29cm 단축되어 가장 큰 감소효과를 보였다 3 포장도복은 무처리 5에 비해 IBP+Inabenfide구는 0으로 도복이 발생하지 않았으며, 수량은 무처리에 비해 도복이 발생하지 않았던 IBP+Inabenfide구가 5% 증수되었다 This study was conducted to investigate the effects of plant growth regulators, 2,4~D, IBP, and Inabenfide, on growth responses and lodging resistance of rice in cultivation of the waterlogging direct seeding The Chuocheong cultivar of Japonica type was used Growth responses itcluding culm leagth and lodging index etc and rice yields were measured at major growth stages and harvest time Culm length was the shortest in the treatment of IBP + Inabenfide, shortening it to 186 cm compared to that of the control, showing the best application effect of the plant growth regulator The length of internode between third and fourth internodes of lower culm was shortened to 3.5 cm with treatment of IBP + Inabenfide compared to that of the control and it between fourth and fifth internodes was shortened to 29 cm with treatment of Inabenfide, showing the best effectiveness. Lodging was not appeared in treatment of IBP + Inabenfide compared to 5.0 of the control Resulting to reduciag the lodging, rice yield was increased more with treatment of IBP + Inabenfide, to about 50%, than that of the control