코디세핀이 마우스 복강 대식세포에서 전염증성 사이토카인의 생성에 미치는 영향

서민정(Min-Jeong Seo),강병원(Byoung-Won Kang),김민정(Min-Jeong Kim),이혜현(Hye-Hyeon Lee),서권일(Kwon-il Seo),김광혁(Kwang-Hyuk Kim),정영기(Yong-Kee Jeong) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.1

본 연구는 동충하초(Cordyces militaris) 유래의 기능성 물질인 코디세핀의 면역활성을 검증하기 위하여 C57BL6 마우스 복강 대식세포를 이용하여 코디세핀이 대식세포의 활성화에 미치는 영향에 대하여 시험하였다. 그 결과 LPS에 의해 유도된 마우스 복강세포는 코디세핀의 작용에 의해 IL-1β, IL-12, TNF-α의 염증성 사이토카인의 생성이 증대되어 초기 염증매개 반응을 유도하여 선천면역반응의 활성화와 그리고 면역작용에 있어 후기 적응면역의 전환으로의 T 림프구의 활성화가 예상된다. 또한 IL-6의 생성증대로 활성화된 T 림프구에 의해 B 림프구의 항체생성반응을 매개하는 면역반응도 상승할 것으로 사료된다. 그리고 대식세포에 의한 염증반응에서 염증매개인자인 NO와 H₂O₂의 생성을 증대시킴에 따라 대식세포의 독성작용을 활성화시켜 염증반응을 효과적으로 유도할 것으로 보이며, 또한 H₂O₂의 후기 생성을 저해하였는데 이는 염증반응에 유도될 수 있는 세포의 손상으로부터 세포를 보호할 수 있을 것으로 사료된다. 따라서 코디세핀은 외부인자로부터 염증매개성 면역반응의 증강작용을 나타내는 것으로 사료된다. The effect of cordycepin purified from Cordyceps militaris on macrophage activation was investigated in peritoneal macrophages isolated from C57BL6 mice. Lipopolysaccharide-induced mouse peritoneal cells showed that cordycepin treatment increased the expression of the inflammatory cytokines interleukin (IL)-1β, IL-12, and tumor necrosis factor-α (TNF-α), leading to early inflammation-mediated reactions, the activation of immunological responses, and T lymphocyte activation. T lymphocytes, activated by a greater production of IL-6, resulted in antibody-generating immune reactions, suggesting that cordycepin was effective at inducing immunological responses. Consistent with the increase in the inflammation-mediating factors including nitric oxide (NO) and hydrogen peroxide (H₂O₂), the toxic response of macrophages was activated and effectively induced inflammation. These findings demonstrate that cordycepin is involved in reducing cell injury provoked by inflammatory reactions. Therefore, these results suggest that cordycepin treatment of mouse peritoneal cells induces inflammation-mediated immunological responses and immunostimulation.

연산동에서 집단발생한 장티브스환자에 대한 세균학적 관찰

李潤日,金慶淑,金明淑,黃基銑,李鍾訓 中央醫學社 1973 中央醫學 Vol.25 No.3

An attempt was made to isolate the causative agent from a group of febrile patients in a construction camp, where the endemic was occurred in a fashion of explosive, and it seemed to be a salmonellosis on the clinical symptoms. Direct rectal swabs and' food samples were taken repeatedly from febrile patients, and only the rectal swabs from the healthy persons to obtain the bacteriological specimens. Sera were also obtained from both healthy persons and febrile patients. Water of glen which penetrates through the camp could be worthy of notice as an infectious source, because one chronic typhoid carrier was found in a slum which was located on a steep ascent just outside of the camp. Typhoid bacilli was isolated from the specimens of 17 patients (58.8%) out of 29 febrile patients, and from 5 out of 92 healthy persons. Widal agglutinin rise was so delayed that it was found not so worthy of examination in the situation of the early management of the patients for prevention of the endemic.

중심 정맥 삽관법에 의한 동정맥루: 증례 및 문헌 고찰

이 근,원형섭,양기창,박철완,김상일,정호성,이종호 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

A 36-year old woman with end stage renal failure and hypertension was transferred from a university hospital to our emergency department due to a enlarging right cervical mass associated with a palpable thrill and a bruit. Over a 2 month period, she required hemodialysis for severe azotemia at a university hospital in Seoul. 3 days after a difficult attempt at a internal jugular cannulation, she developed a progressive swelling in the right lower cervical area. An arch and primary branch arteriogram revealed a arteriovenous fistula between right innominate artery and internal jugular vein with a large intervening pseudoaneurysm. She underwent vascular surgery within 1 week of injury and subsequent postoperative angiogram showed complete resolution of the fistula. This rare condition is reported with a review of the literature.

甲狀腺 홀몬의 受容體에 關한 硏究

李民化,安鳳煥,李起榮,朴鐘一 全南大醫大 1980 전남의대학술지 Vol.17 No.1

Primary Empty Sella 증후군 1례

이홍준,손일진,정은경,문경래,박상기,박영봉 조선대학교 1993 The Medical Journal of Chosun University Vol.18 No.2

The pimapty sella syndrome results from and extension of the subarachnoid space into and intrasellar position with subsequent remodeling of the sella turcica and flattening of the pituitary gland. We experienced a case of primary emty sella syndrome in a 14-year-old male patient who was hospitalized because of short stature and micropenis. The endocrinologic studies revealed growth hormone and gonadotropin hormone deficiencies. The magnetic resonance imaging showed sella turica filled with CSF and flattened pituitary glad. The patient was followed-up with growth hormone therapy and the result was favorable.

대장암에서의 nm23과 CD44 단백질 발현에 대한 면역조직학적 분석

신동일,이기형,윤충,이주희 대한대장항문학회 1997 Annals of Coloproctolgy Vol.13 No.1

비선형 캐리어 제어 부스트 정류기 특성에 관한 연구

이제환,전기영,조정민,한홍일,한경희 明知大學校 産業技術硏究所 2000 産業技術硏究所論文集 Vol.19 No.-

Average-current mode controlled boost rectifier is most universally used at present, While the method gives high power factor, low harmonics distortion, has drawbacks such as 3-feedback loop, complex component, difficulty of control-circuit design, and high cost. Research for improving these problem has been carried out, and Nonlinear Carrier(NLC) Control method is representative. This control method has easy algorithm for control, and its feedback loop and control components are reduced. In this paper, the control method was evaluated by simulation through Matlab/Simulink, and experimented with 550W boost rectifier. As the result, that can get high power factor above 0.95 and low harmonic distortion was confirmed. Also, a good dynamic response on line and load variation was confirmed.

한국인의 하악 구치의 근관 형태에 관한 후향성 연구

정일영,금기연,이승종,이찬영 大韓齒科保存學會 1997 Restorative Dentistry & Endodontics Vol.22 No.2

A retrospective in vivo study of endodontically treated mandiblar molars was conducted. The radilographs and clinical records of mandibular molars that had been treated endodontically in the Dental hospital of Yonsei University from 1986 to July 1997 were reviewed and categorized. A total of 1751 cases were studied and following results were obtained. 1. The number of root canals in mandibular first permanent molar in d Korean population revealed three in 53.2%, four in 23.0%, two in 18.4%, C-shaped canal in 5.0%, and one in 0.4%. 2. In mandibular second molar, three canals was observed in 53.3%, four in 22.2%, two in 14.9%, C-shaped canal in 9.1%, and one in 0.6%. 3. In the results of the percentage of 4 canals of mandibular first molars at various age, before age 20, 37.7% was obtained whereas 19.3% after 30. 4. 7.94% of mandibular first molars examined were found to have three roots. 5. The mandibular second molar had C-shaped canals in 9.1%.

Colletotrichum gleosporides에 의한 枸杞子 ·炭疽病의 發生 및 藥劑防除에 關한 硏究

朴瑞基,陳日斗,李相來 順天大學校 1985 論文集 Vol.4 No.1

最近 拘杞子의 主要 栽培地인 忠南 靑陽과 全南 珍島地方의 拘杞子열매에 큰 被害를 주고 있는 拘杞子·炭疽病의 被害狀況 및 病原菌의 여러 가지 特性을 調査하고 本 病에 대한 여러 가지 炭疽病藥劑의 防除效果를 調査한 結果는 다음과 같다. 1. 拘杞子·炭疽病의 被害는 珍島地方品種이 栽培되고 2週間隔으로 殺菌劑를 撒布하는 珍島地方 栽培圃場에서 보다 靑陽地方品種이 栽培되고 약 1週間隔으로 殺菌劑를 撒布하는 靑陽地方의 栽培圃場에서 더 컸으며, 두 地方品種이 함께 栽培되고 殺菌劑를 전혀 撒布하지 않은 順天地方의 圃場에서 珍島地方品種보다 靑陽地方品種의 被害가 훨씬 더 컸다. 2. 罹病열매에서 分離된 病原菌은 colletotrichum gleosporides이었는데 本 菌의 菌絲發育適溫은 25℃, 發育溫度範圍는 10∼35℃이었고, 胞子發芽適溫은 30℃, 發芽溫度範圍는 10∼35℃이었다. 3. 本 病原菌은 無傷보다 有傷으로 接種하였을 경우 病原性이 훨씬 더 컸다. 즉 有傷接種에서는 靑陽과 珍島地方品種 모두에 病原性이 나타났고, 病斑의 발육이 진전되었으나 無傷接種에서는 靑陽地方品種에만 病原性이 나타났고 珍島地方品種에는 病斑이 形成되지 않았다. 4. 室內實驗에서 7個의 炭疽病藥劑에 대한 菌絲發育의 抑制效果는 캡타폴과 베노밀에서 가장 좋았으나, 胞子發芽의 抑制效果는 디치,타로닐,캡타폴에서 가장 좋았다. 5. 圃場實驗에서 供試藥制에 대한 防除效果는 캡타폴에서 가장 좋았고, 處理時期와 回數에 따른 防除試驗에서, 珍島地方品種은 2回以上處理區의 모두에서 3%以下의 낮은 罹病果率을 나타냈으나, 靑陽地方品種은 1週間隔 5回의 處理區에서만 5%의 罹病果率을 나타냈고 2回處理區의 모두에서 20%以上의 높은 罹病果率을 나타냈다. 한편 靑陽地方品種의 2回 處理時는 어린열매의 감염時期에 處理하는 것이 더 效果的이었다. Recently tea tree-anthracnose has caused severe damage to tea tree fruits in Cheungyang area, Chungnam province and Jindo, Chonnam, principal cultivation districts of tea tree(Lycium chinense M.). Thus present study was conducted to investigate the damage degree of the disease, the various characteristics of the fungus and the control effect of various fungicides to the disease. Damage by this disease was more severe in fields of Cheungyang cultivating the Cheungyang local variety and spraying the fungicide at interval of a week than that of Jindo cultivating the Jindo local variety. and spraying the fungicide at interval of two weeks. In fields of Suncheon cultivating the two varieties and no spraying the fungicide, Cheungyang local variety showed more severe damage than Jindo one. Fungus isolated from the infected fruits was identified as Colletotrichum gleosporides. Mycelial growth of the fungus was best in temperature of 25℃ and it grew in temperature range of 10∼35℃,and conidial germination in 30℃and it geminated in 10∼35℃. Pathogenicity of the fungus was greater on wounded fruits than nonwounded ones:disease lesions on wounded fruits not only developed rapidly, but also was formed in both Cheungyang local variety and Jindo one, while nonwounded fruits in only Cheungyang one. In laboratory tests with seven frngicides, inhibition for mycelial growth of the fungus was the most effective in Captafol and Benomyl, but conidial germination in Dichi, Taronyl and Captafol. In field tests, control effect of the disease according to tested fungicides was best in Captafol. In control tests according to the time and the number of application, Jindo local variety showed percent fruits infected under 3% in all the application plots of the two times and above, whereas Cheungyang one ca. 5% only in the application plots of five times at interval of a week and above 20% in the all application of two times. In Cheungyang local variety, if fungicide was apaplied two times, it was more effective in application to the early infection of the younger fruits.

이황화탄소 폭로가 혈압과 콜레스테롤치에 미치는 영향

洪起天,李恩一,金光鍾 고려대학교 의과대학 1994 고려대 의대 잡지 Vol.31 No.3

To study the effects of exposure to carbon disulfide on blood pressure and serum cholesterol level, we gathered the informations from file of the periodic health examination for occupational disease, the periodic health examination for general disease and medical records in, Wonjin factory clinic. The study subjects were 266 workers who had worked in spinning department as exposure group, and 207 male office workers and 59 female office workers as non-exposure group. The results are as follows; 1. The slope coefficients of systolic blood pressure(as dependent variable) and age and exposure duration(as independent variable) were 0.013 and 0.066 respectively in exposure group. And the slope coefficients of diastolic blood pressure(as dependent variable) and age and expoure duration(as independent variable) mere 0.010 and 0.051 respectively in expoure group. 2. The slope coefficients of systolic blood pressure(as dependent variable) and age and tenure duration(as independent variable) were 0.019 and 0.009 respectively in male unexposure group, and were 0.032 and 0.018 respectively in female unexposure group. And the slope coefficients of diastolic blood pressure(as dependent variable) and age and tenure duration(as independent variable) were 0.016 and 0.009 respectively in male unexposure group, and were 0.019 and 0.033 respectively in female unexposure group. 3. The slope coefficient of exposure duration was greater than that of age in exposure group and that of age was greater than that of tenure duration in male unexposure group. 4.There was no significant difference of blood pressure between the exposure and non-exposure group. 5.The slope coefficients of Serum cholesterol(as dependent variable) and age and exposure duration(as independent variable) were 0.03 and 0.035 respectively in exposure group. The slope coefficients of serum cholesterol(as dependent variable) and age and tenure duration(as independent variable) were 0.102 and -0.012 respectively in male unexposure group. And the slope coefficients was very low in female unexposure group. 6. The slope coefficient of exposure duration was similar to that of age in exposure group, and only age was positively related to serum cholesterol in male unexposure group.