農村地域 綜合開發事業의 課題와 展望

崔秉翼,尹畯相,安相根 公州大學校 地域開發硏究所 1995 地域開發硏究論叢 Vol.3 No.1

SummaryThis study was carried out to review the development polices implied to Korean rural area since the late 1940s. Policies such as Community Development(C. D), Pilot Village Construction Project, Saemaul Projects, Integrated Rural Development planning, and Village Reconstruction Project, and so on. Cases were also reviewed in several areas through literatures. Problems found and alternatives recommended are as follows: 1. Not enough time(several months ∼ a year) is spent for to set up master plans. 2. Little cooperation system is established among relevant organizations. 3. A pilot project with long time(3 ∼ 5 years) in planning is needed for theoretical and practical advancement of the study field.

5-fluorouracil이 결막fibronectin에 미치는 영향 : Immune Electron Microscopic Findings 배양하 면역전자현미경소견

최준규,송기영,고명규 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.1

The success of the filtering in glaucoma is dependent n the patency of the outflow pathway of the aqueous humor from the anterior chamber to the filtering bleb. This is influenced by many factors such as surgical technique, physiologic and pharmacologic factors. Most of the failure of the filtering bleb formation has resulted from the scar formation of teh bleb. This is mainly derived from the fibroblast proliferation which blocks the patent bleb site. The purpose of this study is to investigate the effect of the 5-FU on the extracellular matrix. This involves mainly fibronectin excreted from in vitro, by means of the immunocytochemical stain, at the ultrastructural level. The rabbit conjunctival fibroblast and blood vessel inteh conjunctival stroma were excised and placed in a culture dish containing D-MEM and fetal calf seru. After 2nd week cultivation in 5% CO₂, and 95% air humidified at 37℃, the 5-FU(0.5mg/L) was added. At the 4th week,the culture was terminated for immunocytochemical electron microscopy. The tissue was stained with antifibronectin antibody, followed by incubation with biotin-conjugated goat anti-rabbit IgG. After labelled with HRP-labelled avidin, the tissue was stained with Diaminobenzidine. The fibroblast in the control group showed an indented nucleus with prominent nucleoli, and numerous cytoplasmic inclusions within the cytoplasm. In addition, the cytoplasmic surfacr showed numerous villous processes. However, the plasma membrane was ruptured, and the microorganelles underwent degenerative changes in the fibroblast treated with the 5-FU. The immunoelectorn microscopy revealed an absent or weak reaction product on the vascular wall and plasma membrane of the fibroblast in the 5-FU treated specimen. In short, the basement membrane of the 5-FU added cultured cel revealed a reaction product that was faint when compared with the strong reaction product in the control group. These results suggest, that 5-FU, in a cultured fibroblast and vessel of the conjunctiva, strongly suppresses the excretion of the extracellular matrix, particularly fibronectin. These results suggest, that the application of 5-FU in a filtering operation may be useful for the success of glaucoma surgery, by preventing the secretion of the extracellular matrix, particularly fibronectin.

배양된 망막색소 상피세포의 탐식기능

고명규,최준규,김동섭 한양대학교 의과대학 1991 한양의대 학술지 Vol.11 No.2

This study was performed to observe the phagocytosis of latex bead in cultured human retinal pigment epithelium (RPE). RPE cells were isolated from retina of enucleated human eye and 5 to 7㎛ diameter latex beads were added to culture media of human RPE cells at 50th day of culture. Phagocytic processes were observed serially by phase-contrast microscopy and ight microscopy up to 58 days. After a latent period of about 16hours, latex particles were rapidly phagocytized by cultured human RPE cells. These result may indicate that PRE cells can be cultured up to 58 days without subculture and phamocytic property of RPE remains an that time.

전국 10개 종합병원 중환자실 환자의 직장내 Vancomycin-resistant Enterococci(VRE) 보균율, 보균의 위험요인 및 유전적 다양성 조사

정희진,송준영,엄중식,김우주,최선주,최정현,이혁,정문현,이광미,신동현,손보라,최종성,우흥정,권정아,이경원,최강원,박승철 대한감염학회 2002 감염 Vol.34 No.5

저열량영양식품 섭취에 따른 비만여성의 체중감량 효과에 관한 연구

박순영,최중명,김동현,최경식,최봉근,윤태영,유동준 한국보건통계학회 2002 보건정보통계학회지 Vol.27 No.1

In the method of obesity treatment, Dietary method, Exercise method, Behavior method, and Drug method, Dietary method is the most important control method of obesity. The aim of this study is that evaluate the weight reduction effect of the specific low calorie diet products (KSD I and 2) in obese women. Twenty five women who were volunteer participated in this study. They have no heart diseases and metabolic diseases. we classified two groups : obese group and standard group. Anthropometric indices were measured in all subject for four times. Venous blood sample were taken to determine the lipid profiles, glucose level, and liver function test. In obese group, body weight were reduced 2.85±1.46kg, but showed statistically non-signicicant. Body mass index were gradually reduced, 1.09±0.93kg/m^2(p<0.05).In blood analyses, total cholesterol were reduced 10.61 ±33.4mg/dℓ ( p<0.05). KSD 2 significantly inhibited maltase, sucrase and α-glucosidase from rat intestine, and porcine pancreatic lipase, although KSD I inhibited only the sucrase.

방전관용 전극개발 및 특성에 관한 연구

안성환,황동원,최준영,송시준,전용우,박원주,이광식 영남대학교 공업기술연구소 2001 工業技術硏究所論文集 Vol.29 No.1

This paper describes the development of sputtered tungsten electrode for discharge tube by making use of Sputtering method using plasma. The Xe discharge tube was designed and fabricated by using sputtered tungsten electrode, and the ICCD(Intensified Charged Coupled Device) and monochromator were used in order to observe the discharge image distribution and to measure the signal intensity. As a result, the emission light form the electrode was spreaded almost uniformly over a wide wavelength band, and the discharge image distribution and the signal intensity show quite a uniform distribution. The emission light from the Xe discharge tube was peaked at 488[nm] and 648[nm].

자가방수액이 가토 결막조직에 미치는 영향

신경학,고명규,최준규 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.2

It has been well known that the failure of the trabeculectomy is associated with the migration and proliferation of the fibroblast at the fistula site, which may be influenced by the aqueous humor. It is not clear whether the aqueous humor nas growth factor or inhibitory factor. This experiment was designed to elucidate the effect of the aqueous humor on the conjunctiva in rabbit. The aqueous humor was injected into the subconjunctival area in the rabbit, twice weekly upto for 1 minth. In addition, the autologous conjunctival tissue was implanted into the anterior chamber of the fellow eye, which was observed for three months. The conjuntiva, which was treated by the aqueous humor in situ and in anterior chamber, was examined with electron microscopy. The conjunctiva, treated with autologous aqueous humor, showed the infiltration of the inflammatory cells and fivrin in the substantia propria. These findings may be affected by the disruption of the blood-aqueous barrier or chemoattractant activity of the aqueous humor. The conjunctiva, implanted into the anterior chamber, disappeared with fragmentation or was shrinked in appearance. The conjunctival epithelial cells showed the degeneration with widened intercellular space, slightly protruded villous projection into intercellular space and separation of desmosome between adjacent cells. The substantia propria was well preserved with both the endothelial and pericyte cell uninterruptedly lined with basement membrane. The widened intercellular space and slightly projected villous projection may be influenced by the growth activity in the aqueous humor. However, the inhibitory activity in the aqueous humor may contributetothe degeneration in teh superficial epithelium and no further growth of the conjuntival tissue. These fundings may be summed up by the growth activity of the plasmoid aqueous by disruption of blood-aqueous barrier following surgical manipulation and by inhibitory activity in stabilized aqueous humor. The influence of aqueous homor on the cytologic or histologic pattern will depend on the biochemical components of the aqueous humor.

선천 섬유화 증후군 1예

김동섭,신정철,고명규,최준규,장세진 한양대학교 의과대학 1998 한양의대 학술지 Vol.18 No.1

The congential fibrosis syndorme is rare congenital disorder which is characterized by blepharoptosis, varying degrees of limitation of eye movement, especially in vertical gaze, and abnormal head position. These clinical findings result from the fibrous replacement of normal contractile extraocular muscle tissue. The authors report one sporadic case of congential fibrosis syndrome. The 14 year-old-girl showed bilateral ptosis, fixed ocular misalignment in downward gaze, limited ductions and versions, and characteristic chin up position. The surgical management attempted to achieve functional readjustment of the ocular alignment and lid position and to correct cosmetically unacceptable abnormal head position. Result of histopathologic examination obtained at surgery shows replacement of the extraocular muscles, conjuctiva and Tenon's capsule by collagen and dense fibrous tissue. The surgical outcome is considered satisfactory when compared with preoperative status although the limitation of vertical eye movement remains unchanged after surgery.

Sodium Hyaluronate가 가토 결막상피에 미치는 형태학적 연구

유석찬,고명규,최준규 한양대학교 의과대학 1991 한양의대 학술지 Vol.11 No.1

This study was carried out whether sodium hyaluronate may affect the mucous layer of the tear film and surface layer of the conjunctival epithelium by histochemical electron microscopy. Sodium hyaluronate was injected into the subconjunctival tissue of five rabbits twice a week for 10 weeks. The excised specimen was treated with ruthenium red to enhance the presence of glycosaminoglycans of the surface layer of the conjunctiva. From the histochemical electron microscopic point of view, sodium hyaluronate did not improve the content of glycosaminoglycans density in the tear film over the surface of the conjunctival dpithelium. In addition, there was no evidence of epithelial lesions such as desquamation or stratification. However, the mucus layer was intermingled with secretory granules of goblet cell with the mucus layer spread over glycocalyx. The results of this study indicate that sodium hyaluronate injected into the subconjunctigval layer has little effect on the glycosaminoglycans density over the conjunctival epithelium in rabbit.

P3 Promoter of Bovine Papillomavirus Type - 1

Choe, Joon Ho,Peter Vaillancourt,Michael Botchan 한국유전학회 1988 Genes & Genomics Vol.10 No.4

In attempt to map the structure of transcripts which contain coding sequence from the E1 and E8 ORFs of BPV-1, we have identified a new promoter which is internal to the E1 ORF. S1 nuclease protection and primer extension analysis of poly(A) cytoplasmic RNA from ID 13 cells reveal the presence of a pair of 5′ ends at nucleotides 886 and 896 which correlate well with the 5′ ends of a class of cDNA clones. In order to determine whether these 5′ ends corresponded to a functional promotor, the BPV-1 Pst I fragment from 576-1299 was tested in an in vitro transcription assay using HeLa cell extract. Primer extension analysis of the in vitro transcription products showed the presence of two cap sites which were the same as those observed for in vivo mRNA, and whose transcription was α-amanitin sensitive. Using a set of overiapping, uniformly labelled SP6 probes from nucleotides 576-1299 we showed by RNase protection analysis that the vast majority of transcripts which initiate from this promotor use the splice donor at nucleotide 1235 previously mapped. This confirms that the most abundant class of P3 cDNAs uses this leader exon. These transcripts are moderately abundant relative to the bulk of the BPV-1 mRNAs: the P3 leader exon-containing transcripts are estimated to be about 5-fold less abundant than the set of E6, E7 and E6/E7 transcripts which contain exons which use the splice donor at nucleotide 865. Analogous promotors and leader exons located in the 5′ region of the E1 ORF have been identified in HPV-6 and HPV-11.