백서 척수후근절로부터 슈반세포의 효과적인 체외 배양법

김성민,이종호,김남열,안강민,최원재,최시호,차미주,이주영,황순정,장정원,명훈,최진영,서병무,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

Schwann cells(SCs), an important component of the peripheral nervous system, intract with nerous to mutually support growth and replication for the peripheral nerve regentation. Recently, ading SCs to the lumen of guidance channel is widely tried to improve regeneration or to make regeneration possible over otherwise irreparable gaps. however, it is not easy to isolate and multiplicate SCs as much as enough to help the axonal regeneration. For the allogeneic SCs source for tubular nerve guidance, we developed a little bit improved technique of harvesting and multiplicating SCs. by culturing dispersed dorsal root ganglia in specially designed medium with growth factors and serial processing, we repeatedlly generate relatively homogenous SC cultures. Our technique was compared with other methods of literature using immunostaining methods such as GFAP, S100, BDNF and the total SC count assessment at different time interval after primary culture.

액체금속로 KALIMER의 가동중검사 및 보수 개념설계

주영상,김석훈,이재한 한국비파괴검사학회 2004 한국비파괴검사학회지 Vol.24 No.2

가동중검사와 보수는 원자력발전소의 원자로계통설계에서 매우 중요한 설계개념이다. 액체금속로 KALIMER의 가동성 검증을 위해서 기계계통 설계에 가동중검사와 보수개념이 반영되어야 한다. 본 연구에서는 KALIMER의 안전성과 신뢰성을 확보하기 위하여 KALIMER의 개념설계 단계에 필요한 가동중검사와 보수의 기본 개념을 설정하였다. 액체금속로 가동중검사 규정인 ASME Section ⅩⅠ Division 3를 반영하고 KALIMER의 설계특성을 고려하여 원자로계통과 주요부품의 가동중검사와 보수에 대한 방법과 요건을 설계하고 기술하였다. The design concepts of in-service inspection and maintenance are very important for the reactor system design of the nuclear power plant. The strategy of in-service inspection and maintenance should be reflected in the mechanical system design for the verification of the operability of liquid metal reactor KALIMER. In this paper the fundamental approaches of the in-service inspection and maintenance of the KALIMER are established to ensure the safety and reliability of the reacter system. The general method and requirement of the in-service inspection and maintenance for the reactor system and components are proposed and described to satisfy the intents of the ASME Section XI Division 3 and the design characteristics of KALIMER.

허혈성 심질환에서 나타나는 황상화지수, 사이토카인 및 염증지표의 변화

전상훈,안지훈,방덕원,온영근,현민수,김성구,권영주 순천향대학교 의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

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선천성 후비공 폐쇄증 : 7예의 분석

김창훈,이정권,최윤석,유신영,윤주헌 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.3

불안의 형성과정에 있어서 뇌 Cholecystokinin의 역할

김영훈,심주철 대한생물치료정신의학회 1995 생물치료정신의학 Vol.1 No.2

Cholesystokinin(CCK) is one of the most abundant neuropeptides and its receptors are widely distributed throughout the central nervous system, especially in hypothalamus, limbic system, basal ganglia, hippocampus and cortex. CCK is co-localized or interacts with dopaminergic, noradrenergic, GABAergic and serotonergic neurotransmitter systems. lts functional roles are not fully defined, but it appears to mediate anxiety and it may participate in satiety, nociception and drug withdrawal. CCK/dopamin neurons in ventral tegmental area project their long pathways to limbic system and striatum and their roles in the pathogenesis of dopamine-related disorders such as schizophrenia are shortly described in this article. Cholecystokinin tetrapeptide(CCK-4) is suggested to have a potent panicogenic property in human. Both clinical and animal research suggests that ?? receptors mediate the panicogenic effects of CCK-4 and their antagonists provide an important advance in the treatment of anxiety disorder. The possible involvement of CCK in the pathogenesis of panic disorder is described briefly. The antagonistic action of imipramine on CCK-4-induced panic and that of 5-HT₃ on CCK release in nucleus accumbens are discussed. As described above, there is growing speculations that CCK might be implicated in the neurobiology of anxiety and its receptor agonists could be used in the treatment of anxiety in future.

정상교합자의 Ⅲ급 부정교합자의 저작근 근전도에 관한 연구

박영국,이기수,주보훈 대한치과교정학회 1991 대한치과교정학회지 Vol.21 No.1

The purpose of the present study was to investigate the differences of EMG activity of the masticatory muscles between normal occlusion and Class Ⅲ malocclusion during various jaw functions. 46 subjects of 18.4-25.7 years were employed in this study: 26 subjects were normal occlusions, and 20 subjects were Class Ⅲ malocclusions. The EMG data from the anterior and posterior temporal, anterior and posterior masseter muscles in both sides as mandibular elevators and supra-hyoid muscle group(close to the anterior belly of digastric muscle in right side) as mandibular depressor were recorded with the Medelec MS 25 electromyographic machine. The EMG recordings were analyzed during mandibular rest position, maximal biting, mastication with chewing gum, and swallowing of peanuts. All data were recorded and statistically processed. 1.The maximal mean amplitude of the anterior temporal muscle was stronger significantly in Class Ⅲ malocclusion than in normal occlusion, and then the posterior temporal was weaker during mandibular rest position. 2.The maximal mean amplitudes in the anterior and posterior temporal muscles and the anterior masseter muscle of Class Ⅲ malocclusion was weaker significantly than that of normal occlusion during maximal biting. 3.During mastication of the chewing gum, the maximal mean amplitudes of Class Ⅲ malocclusion was weaker significantly than normal occlusion in the anterior and posterior temporal muscles of the working side, and the duration of Class Ⅲ malocclusion was longer in the anterior temporal muscles of both sides and the posterior temporal and the anterior masseter muscle of the balancing side. There were significant increasings of the latency in balancing anterior temporal, working posterior temporal muscles and supra-hyoid muscle group of Class Ⅲ malocclusion. The silent period durations was 16.36 ms in Class Ⅲ malocclusion while 10.76 ms in normal occlusion, which was statistically different(P<0.05). 4.At swallowing of peanuts, the maximal mean amplitude of Class malocclusion was weaker significantly in the posterior temporal muscle than that of normal occlusion. There was no significant difference of duration between normal occlusion and Class Ⅲ malocclusion. 5.The muscle activities of Class Ⅲ malocclusion had a tendency of decrease less than normal occlusion. And then the muscle activities of the anterior temporal and anterior masseter muscles in Class Ⅲ malocclusion showed the tendency of the increase more than other muscles of Class Ⅲ malocclusion.

mGA를 이용한 웨이블렛 퍼지 모델링

유진영,이연우,주영훈 국립7개대학공동논문집간행위원회 2002 공업기술연구 Vol.2 No.-

In this paper, we propose a new approach to Wavelet Fuzzy Modeling using messy genetic algorithm(mGA). mGA has more effective and adaptive structure than sGA with respect to using the changeable-length string. We build a fuzzy system model which is equivalent to the wavelet transform after identifying the coefficients of wavelet transform. We can obtain an accurate system model with a small number of coefficients due to the energy compaction property of the wavelet transform. And it means that we can construct a fuzzy system model with a small number of rules. By the conventional fuzzy modeling method, a problem might arise that a function with abrupt changes can hardly be modeled precisely. Hence, we present a solution to this problem by the advantageous property of the wavelet transform. We demonstrate the superiority of our proposed fuzzy system modeling method over the previous results by modeling a highly nonlinear function.

정신분열병 환자 가족을 위한 한 정신교육적 가족치료 모형의 효과

이영호,심주철,이상경,서영수,김용관,김규수,김영훈 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.3

연구목적 : 본 교실에서 정신분열병 환자 가족을 대상으로 시행 중인 장기 정신교육적 가족치료모형을 소개하고, 이모형이 가족의 대처방식과 우울증상, 환자가 자각하는 가족의 정서적 지지와 우울증상, 재입원율 및 사회재활상태에 미치는 영향을 평가하였다. 방 법 : 연구대상은 DSM-IV 진단기준에 의해 정신분열병으로 진단받고 외래 통원치료를 받고 있는 환자와 그 보호자였다. 교육군은 전체 19회 교육 중 최소 ⅔이상 참여한 11명의 환자와 가족이었고 비교육군은 부산소재의 종합병원에서 가족교육을 받지 않고 통원치료만을 받았던 11명의 환자와 가족이었다. 정신교육적 가족치료 모형의 효과는 가족의 대처방식과 우울증상, 환자가 자각하는 가족의 정서적 지지와 우울증상, 재입원율, 사호재활 실태로 나누어 평가했다. 평가도구는 The Ways of Coping Checklist의 국내번안용, Family Emotional Support Questionnaire for Transaction. Beck Depression Inventory를 이용했다. 재입원율 및 사회재활 실태에 대한 평가는 환자 및 가족과의 면담을 통해 조사했다. 결 과 : 본 연구의 정신교육적 가족치료 모형 실시 후 가족의 대처방식 중 적극적 대처는 교육군운 의미있게 증가한 반면 비교육군에서는 변화가 없었다. 소극적 대처 교육군은 의미있게 감소했으나 비교육군에서는 변화가 없었다. 가족치료모형 실시 전 가족들은 양군 모두 심한 우울증상을 갖고 있었다. 가족치료모형 실시 후 가족의 우울증상은 교육군에서 감소, 비교육군은 증가를 보였으나 통계적 유의성은 없었다. 환자가 지각하는 가족의 정서적 지지 평가척도점수는 교육군은 의미있는 증가를 보였으나 비교육군에서는 변화가 없었다. 환자의 우울증상의 교육군에서는 현저한 감소를 보인 반면 비교육군은 상반되게 증가했다. 가족의 적극적 대처가 환자가 자각하는 정서적 지지의 증가 및 우울증상 감소와 의미있는 상관관계를 나타냈다. 또한 교육군의 환자들이 비교육군의 환자들보다 장기가족교육 종료시점과 9개월 경과 후의 추적 평가에서 재입원율이 현저히 낮았고, 사회재활상태도 좋았다. 결 론 : 본 연구에서 실시된 장기 가족교육모형은 가족의 대처방식을 향상시키고, 우울증상의 감소를 가져오며, 환자가 자각하는 가족의 정서적 지지 향상 및 우울증상의 호전을 가져오고, 재입원율의 저하 및 사회재활의 향상을 보이는 것으로 나타났다. Objectives : This study was performed to introduce a psychoeducational family therapy model for the families of schizophrenic patient and to investigate the effect of this model on the changes in coping style and depressive symptoms of the family members, and in perception of emotional support by families and depressive symptoms of patients. Methods : Subjects were schizophrenic out-patients, who fufilled DSM-IV diagnostic criteria, and their families. The members of "education group" were 11 families and patients who participated in more than two thirds of 19 sessions of the program. The members of "non-education group" were 11 families and patients who had no experience of family education and were treated in a general hospital in Pusan. Clinical ratings were performed using by The Ways of Coping Checklist, Family Emotional Support Questionnaire for Transactions, and Beck Depression Inventory. Results : Coping style was changed positively, in education group but not in non-education group. The depressive symptoms of families were decreased in educated group and increased in non-educated group, although there were no statistical significance. In education group, patients felt much better emotional support by their families than patients in non-education group did. At the same time, their depressive symptoms were significantly reduced. The patients from education group were on more successful rehabilitation than those from non-education group were. Conclusion : Our results suggest that this psychoeducational family therapy model would be helpful to induce the positive changes in coping styles and reduce the depressive symptoms of family members, and it could also induce the reduction of depressive symptoms of schizophrenic patients and help their rehabilitation.

地骨皮 EA分劃의 CCl₄損傷肝에 대한 保護作用

曺永周,金聖勳 대한동의병리학회 1997 동의생리병리학회지 Vol.11 No.2

肝에 對한 東醫學的 實驗 硏究는 肝이 代謝物質에 의한 中毒에 敏感하게 反應하여 肝細胞의 變性, 壞死, 脂肪蓄積, 肝酵素의 漏出 등의 肝障碍가 나타나는 점에 着眼하여 肝毒性 誘發 物質인 CCl₄등의 肝損傷 病態 모델을 이용한 損傷肝에 대한 保護作用 實驗이 主를 이루고 있고, 最近에는 韓藥 혹은 韓方處方의 抽出物에서 補肝作用을 나타내는 主要 物質이 多糖類임이 實驗的으로 밝혀져 이에 對한 硏究도 활발히 進行되고 있다. 이에 본 연구는 대표적인 補肝藥物이라고 할 수 있는 拘杞子의 根皮인 地骨皮가 肝·肺·賢經에 入하고 淸熱凉血 作用이 있음을 勘案하여, in vitro에서 肝保護作用을 screening 하였던 바, 地骨皮 ethyl acetate(EA)層이 가장 有效하여, 本 實驗에서는 in vivo에서 四鹽化炭素(CCl₄)로 肝損傷을 誘發시킨 後 地骨皮 ethyl acetate層과 地骨皮 ethyl acetate層을 다시 SiO₂chromatograph 方法을 利用하여 分離한 細分劃(F-1, F-2, F-3, F-4)을 試科로 使用, 經口投與 後 肝機能과 相關된 各種 血淸分析 및 肝組機檢査 등을 實施하였다. CCl₄中毒 肝損傷에서 albumin과 cholesterol値는 對照群에 비하여 地骨皮 ethyl acetate層 投與群과 DDB 投與群에서 有意性있게 增加되었으며, alanine aminotransferase와 aspartate aminotransferase値는 對照群에 비하여 地骨皮 ethyl acetate層 投與群과 DDB 投與群에서 有意性있게 減少되었다. lactate dehydrogenase値는 對照群에 비하여 DDB 投與군에서 有意性있게 減少되었고, 地骨皮 ethyl acetate層 投與群에서 對照群에 비하여 減少되었으나, 有意性은 나타나지 않았다. 中毒에 의한 肝組織 變化에서는 正常群에 비하여 對照群에서 中心靜脈과 門脈周圍에서 공포성 변성이 나타나고, 細胞內의 核이 濃縮되며, 中性球의 浸潤이 뚜렷하게 나타났으며, 地骨皮 ethyl acetate層에서 投與群은 DDB 投與群 정도로 나타나 肝細胞의 損傷을 抑制하였다. Ethyl acetate層에서 특히 F-3와 F-4 분획 投與群이 對照群에 比하여 ALT, AST에서 모두 有意性있게 減少하였으며, 특히 F-3 投與群이 더욱 有效하였다. In order to evaluate the protective effect of ethyl acetate layer and its subfractions (F-1, F-2, F-3, F-4) of Lycii Cortex Radix (LCREA) on hepatic damage induced by CCl₄, the study was done. The blood chemistry and histological study were done following oral administration with materials. The results were obtained as follows: 1. In the hepatocytotoxicity with CCl₄, serum albumin and cholesterol were significantly increased in LCREA and DDB-treated groups as compared with the data of control. 2. In the hepatocytotoxicity with CCl₄, serum alanine aminotransferase (ALT) and aspartate aminotransferase(AST) were significantly decreased in LCREA and DDB-treated groups as compared with the data of control. 3. In the hepatocytotoxicity with CCl₄, serum lactate dehydrogenase (LDH) tended to decrease in LCREA-treated group with no significance while with significance in DDB-treated group as compared with control. 4. In histological changes of murine liver treated by CCl₄, LCREA inhibited the hepatic damage by CCl₄such as vacuolar degeration, lipid infiltration and pyknosis up to the efficacy by DDB as compared with control. 5. In the hepatocytotoxicity with CCl₄, serum alanine aminotransferase (ALT) and aspartate aminotransferase(AST) were significantly decreased in F-3 and F-4 treated groups as compared with the data of control and also F-3 subfraction was more effective. It was concluded that LCREA had protective effects on the hepatic damage, and especially F-3 isolated from LCREA was more effective, which suggest it is necessary to study more with F3 of Lycii Cortex Radix.

기내배양시 GA₃처리에 의한 들깨의 생육 및 개화조절

최영환,최영훈,강점순,손병구,이영근,주우홍 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

This study was conducted to estimate growth of leaf and shoot and flowering as affected by GA3 treatment in vitro. Plant height was longer as the concentration of GA3 was higher up to 2.0mg/l. Stem diameter was thick up to the concentration of GA3 was 0.1mg/l , but become thinner as the concentration of GA3 was higher. Root weight and root length were well developed in GA3 concentration was 0.5∼2.0mg/l. Number of roots was more in GA3 concentration was 0.5 and 1.0mg/l . The first flowering occurred in 40 days after 0.5 mg/l GA3 treatment. As the concentration of GA3 was higher, flowering was delayed. Number of flowering was significantly increased in GA3 concentration was 0.1 and 0.5mg/l. However, it was gradually decreased after 60 days. Percentage of flowering per plant was highest in GA3 concentration was 0.5mg/l , but it was lower as the concentration of GA3 was increased.