부대토목공사 선시공 공법 적용을 통한 건설환경 개선에 관한 연구

주성락,김효열,임남기 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

This study has been reviewed and analyzed in order to shorten the construction period and save the cost by preparing better construction environment and improving the effectiveness of workers with the application of the pre-accessory civil work method in the construction of an apartment building, which will have to be advanced by continuous study of joint efforts of academy, industry, and government.

관상동맥 질환에서 CD14 유전자형에 따른 세균 및 Heat Shock Protein에 대한 반응의 차이

한주용,최수연,조현주,김화평,강현재,구본권,김남중,김효수,손대원,오병희,박영배,최윤식 대한감염학회 2007 감염과 화학요법 Vol.39 No.1

Background : CD14 is the receptor for lipopolysaccharides and heat shock protein (HSP), which has been suggested being associated with increased risk of coronary artery disease (CAD). We investigated whether the response to infectious agents or HSP is different according to CD14 polymorphism in Koreans. Materials and Methods : Antibody titers to Helicobacter pylori, Chlamydia pneumoniae, and human HSP60 (hHSP60) were measured in 48 patients with stable CAD and in 41 healthy controls by ELISA. CD14 genotype was determined by PCR and high-sensitivity C-reactive protein (hs-CRP) was measured. Results : Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly associated with the presence of CAD. CD14 genotype distribution was 31 TT (35%), 43 CT (48%), and 15 CC (17%). To compare the response to the infectious organism and hHSP60, we divided study population into 3 groups; CAD patients with non-TT genotype (group I, n=30), CAD patients with TT genotype (group II, n=18), and normal controls (group III, n=41). Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly different among 3 groups. Though hs-CRP level was significantly different among 3 groups, post-Hoc analysis showed that hs-CRP level was not significantly different between group I and group II (group I: 1.6[1.1-3.5] mg/L and group II: 0.35[0.1-2.0] mg/L). Conclusions : This study suggests that the inflammatory responses to infectious organisms and HSP do not differ according to the CD14 genotype in Koreans. 목적 : 만성적인 세균 감염이나 자가 면역 반응이 동맥경화와 연관되어 있다는 주장이 제기되어 왔다. CD14은 lipopolysaccharides (LPS)와 heat shock protein(HSP)의 수용체로 C(-260)→T 다형성이 관상동맥 질환의 위험과 관련되어 있다고 제안되어 왔다. 본 연구에서는 한국인에서 CD14 다형성에 따른 LPS와 HSP에 대한 반응성을 염증표지자를 측정하여 살펴보고자 하였다. 재료 및 방법 : 안정형 관상동맥 환자 48명과 정상 대조군 41명을 대상으로 Chlamydia pneumoniae, Helicobacter pylori 항체 및 인체 HSP60 (hHSP60) 항체 역가를 enzyme-linked immunosorbent assay (ELISA) 방법으로 측정하였다. CD14 유전자형은 중합효소연쇄반응을 이용하여 결정하였고 hs-CRP를 측정하였다. 결과 : C. pneumonias 및 H. pylori 항체 양성 여부 및 항체 역가, 그리고 hHSP60에 대한 항체 역가는 안정형 관상동맥 질환의 유무와 유의한 상관관계는 없었다. CD14 유전자형의 분포는 TT 30명(39%), CT 31명(40%), 그리고 CC 16명(21%)이었다. 관상동맥 질환 군에서 TT 유전자형은 38%, 대조군에서는 32%로 TT 유전자형과 관상동맥 질환의 유무와는 유의한 상관관계가 발견되지 않았다(P>0.05). CD14 유전자형에 따라 C. pneumonias와 H.pylori 감염 및 hHSP60에 따른 반응에 차이가 있는지 여 부를 알아보기 위해, 관상동맥 질환 군을 다시 TT 유전자형 군과 non-TT 유전자형 군으로 나누어, 전체적으로 세군에서 항체 양성률과 역가를 비교하였다(I군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, II군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, III군: 정상 대조군). 세 군에서 C. pneumonias와 H. pylori 항체 양성률 및 hHSP60 항체 역가에는 유의한 차이가 없었다. 세 군사이에 hs-CRP 값에 유의한 차이가 있었지만, post-Hoc분석에서 II군의 hs-CRP 값이 I군에 비해 유의하게 높지는 않았다(0.35 [0.1-2.0] mg/L in group II vs. 1.6 [1.1-3.5]mg/L in group I, P>0.05). 결론 : 본 연구는 한국인에서 CD14 유전자형에 따라 LPS나 HSP에 대한 반응이 유의하게 다르지 않음을 시사한다.

반도체소자의 고속마킹검사를 위한 vision system 개발

노영동,주효남,김주식 湖西大學校 工業技術硏究所 2005 工業技術硏究所論文集 Vol.24 No.-

In this paper, we propose the high speed making/surface inspection algorithm, that use adaptive automatic acquisition algorithm and real time matching algorithm of model data. The proposed automatic acquisition algorithm to obtain the adaptive model data extracts the interesting regions to fit in semiconductor characteristics, and create models of several semiconductor regardless of position and type. The proposed teal time matching algorithm uses geometrical pattern matching method to minimize effect of external environment of making/surface and only use semiconductor characteristic information. The proposed system has faster processing time than the conventional method. Also the proposed one has a good performance.

국내외 하수처리장 자동화 현황 조사

김창원,고주형,최광수,허남효,김병군 釜山大學校生産技術硏究所 1999 生産技術硏究所論文集 Vol.56 No.-

하수처리장 운전 제어 및 자동화는 현재 세계적인 추세이다. 자동 제어를 도입함으로써 운전의 효율성 및 안정성 향상, 일관된 운전, 운전비용 절감 등 많은 이득을 기대할 수 있다. 유럽 등 선진국에서는 이미 7,80년대부터 다방면에서 많은 역구가 수행되었고, 80년대 이후 건설된 대부분의 하수처리장에서 용존산소 농도 제어를 비롯한 기본적인 제어를 실제로 적용하고 있다. 그러나 현재까지 국내에는 하수처리장에 자동 제어가 적용된 경우가 없으며, 현장 적용은 몇몇 폐수처리장에 국한된다. 그러나 자동 제어를 통해 얻을 수 있는 이득이 매우 크다는 것이 명백하고, 현재 각 대학 연구에서 활발하게 연구를 수행하고 있으므로, 가까운 장래에 많은 하·폐수처리장에 자동 제어가 광범위하게 도입될 것으로 기대된다. Operational control and automation of sewage works is a worldwide tendency. By introducing automatic control, many benefits like improvement of efficiency and stability, consistent operation an operational cost saving, can be obtained. Lots of process studies have been performed from 7,80s in developed countries, and basic control (e. g. DO control) is having been appplied to almost every plants constructed after 80s. In Korea, no large sewage work is automatically controlled till now, while some limited application for wastewater treatment plants are exist. But it is very clear that automatic control holds great porfits and lots of vigorous researches are being conducted in many universities and laboratiories, so widespread introduction of automatic control to sewage/wastewater treatment plant in near future is expected.

FPD 결함검사를 위한 Vision Inspection System 설계에 관한 연구

박성재,김민수,김준식,주효남 湖西大學校 工業技術硏究所 2005 工業技術硏究所論文集 Vol.24 No.-

A system is designed and developed to acquire accurate images and to detect cell defects of Flat Panel Displays. Detailed analysis and experiments are performed to design and select the lighting, optical and camera sub-system as well as mechanical motion and position control sub-system. Sample images are acquired using the developed system and the processed result shows the good performance in detecting simple cell defects.

Cells Transformed by PLC-Gamma 1 Overexpression are Highly Sensitive to Clostridium difficile Toxin A-Induced Apoptosis and Mitotic Inhibition

( Nam Hyo Jung ),( Jin Ku Kang ),( Jong Soo Chang ),( Min Soo Lee ),( Seung Taek Nam ),( Hyun Woo Jung ),( Sung Kuk Kim ),( Eun Mi Ha ),( Heon Seok ),( Seung Woo Son ),( Young Joo Park ),( Ho Kim ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1

Phospholipase C-γl (PLC-γl) expression is associated with cellular transformation. Notably, PLC-γ is up-regulated in colorectal cancer tissue and breast carcinoma. Because exotoxins released by Clostridium botulinum have been shown to induce apoptosis and promote growth arrest in various cancer cell lines, we examined here the potential of Clostridium difficile toxin A to selectively induce apoptosis in cells transformed by PLC-γl overexpression. We found that PLC-γl-transformed cells, but not vectortransformed (control) cells, were highly sensitive to C. difficile toxin A-induced apoptosis and mitotic inhibition. Moreover, expression of the proapoptotic Bcl2 family member, Bim, and activation of caspase-3 were significantly up-regulated by toxin A in PLC-γl-transformed cells. Toxin A-induced cell rounding and paxillin dephosphorylation were also significantly higher in PLC-γl-transformed cells than in control cells. These findings suggest that C. difficile toxin A may have potential as an anticancer agent against colorectal cancers and breast carcinomas in which PLC-γl is highly up-regulated.

<i>Spirodela polyrhiza</i> extract modulates the activation of atopic dermatitis-related ion channels, Orai1 and TRPV3, and inhibits mast cell degranulation

Nam, Joo Hyun,Jung, Hyo Won,Chin, Young-Won,Yang, Won-Mo,Bae, Hyo Sang,Kim, Woo Kyung TaylorFrancis 2017 Pharmaceutical biology Vol.55 No.1

<P><B>Abstract</B></P><P><B>Context:</B><I>Spirodela polyrhiza</I> (L.) Schleid. (Lemnaceae), Spirodelae Herba (SH), has been known to relieve inflammation, urticaria and skin symptoms including pruritus, eczema and rash.</P><P><B>Objective:</B> The effects of SH extract on two calcium ion channels, Orai1 and TRPV3, and their potential as novel therapeutics for atopic dermatitis (AD) were investigated. The regulatory role of Orai1 on mast cell degranulation was evaluated.</P><P><B>Materials and methods:</B> The dried leaves of SH were extracted by 70% methanol. Effects of SH extract (100 μg/mL) in an HEK293T cell line overexpressing human Orai1 or TRPV3 were assessed. Ion channel modulation in transfected HEK293T cells was measured using a conventional whole-cell patch-clamp technique. IgE-antigen complex-stimulated mast cell degranulation was measured by β-hexosaminidase assay with morphological observation after treatment with 20, 50 and 100 μg/mL SH extract.</P><P><B>Results:</B> SH extract (100 μg/mL) significantly inhibited Orai1 activity (63.8 ± 0.97%) in Orai1-STIM1 co-overexpressed HEK293T cells. SH extract significantly increased TRPV3 activity (81.29 ± 0.05% at −100 mV) compared with the positive control 2-APB (100 μM), which induced full activation. SH extract inhibited degranulation in IgE-antigen complex-stimulated RBL-2H3 mast cells by decreasing β-hexosaminidase activity (3.14 ± 0.03, 2.56 ± 0.12 and 2.29 ± 0.08 mU/mg, respectively).</P><P><B>Conclusion:</B> Our results suggested that SH extract could treat abnormal skin barrier pathologies in AD through modulation of the activities of the calcium ion channels Orai1 and TRPV3 and inhibition of mast cell degranulation. This is the first report of an herbal effect on the modulation of ion channels associated with skin barrier disruption in AD pathogenesis.</P>

Monitoring of 511 Pesticide Residues in Agricultural Products by LC-MS/MS and GC-MS/MS

Nam Suk Kang,Su Jin Kim,Chan Woong Park,Si Woo Bark,Young Joo Park,Hyo In Kim,Sun Hwa Lee,Jin-Wook Kwon,Woo Seong Kim,Chang Hee Lee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

The remaining pesticide in food is deal with important in international trade, so maximum residual limits (MRLs) are established and managed in many countries. In Republic of Korea, positive list system (PLS) for all of the agricultural products was also introduced for the safe management of residual pesticides and analysis methods have been developed to support it. Therefore, in this study, the residues of 511 pesticides were monitored for 217 domestically distributed agricultural products in Korea by the newly developed multi pesticide residue analysis method. Samples were extracted by the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS). Monitoring result showed 53.9% of frequency of occurrence (117/217) with the total of 88 pesticides determination. Each of frequency was as followings; carbendazim (0.01∼2.4 mg/kg), dinotefuran (0.01∼0.2 mg/kg), pyraclostrobin (0.01∼0.7 mg/kg) and spirotetramat (0.07∼1.75 mg/kg). The amount of pesticides detected in all smaples was lower than MRLs.

Salvage Regimens after Failure of Previous Helicobacter pylori Eradication Therapy: A Systematic Review and Meta-analysis

Hyo-Joon Yang,정혜경,Seung Joo Kang,이용찬,Seon-Young Park,Cheol Min Shin,Sung Eun Kim,임현철,Jie-Hyun Kim,Su Youn Nam,Woon Geon Shin,Jae Myung Park,Il Ju Choi,김재규,Miyoung Choi,Korean College of Helicobacter a 대한상부위장관ㆍ헬리코박터학회 2021 Korean Journal of Helicobacter Upper Gastrointesti Vol.21 No.1

Background/Aims: As antibiotic resistance increases and new first-line therapies emerge, salvage therapies for Helicobacter pylori (H. pylori) eradication failures are becoming more common and complicated. This study aimed to systematically review overall salvage regimens after previous failure of H. pylori eradication. Materials and Methods: A systematic review of randomized clinical trials evaluating salvage therapies after previous H. pylori eradication failure was performed. A meta-analysis was conducted when an adequate number of studies suitable for grouping was found. Results: Overall, 36 studies with 77 treatment arms were identified, and they were highly heterogeneous regarding previously failed regimens and salvage regimens under comparison. Bismuth quadruple therapy after failure of standard triple therapy showed a pooled intention-to-treat (ITT) eradication rate of 75.5% (95% CI, 71.6~79.1%), and the rates were significantly higher with 14-day therapy than 7-day therapy by 9% (95% CI, 2~15%). Levofloxacin triple therapy after failure of standard triple therapy demonstrated a pooled ITT eradication rate of 73.3% (95% CI, 68.4~77.3%). In direct comparison, the two regimens were not significantly different in eradication rates. No study evaluated salvage regimens after the failure of bismuth or non-bismuth quadruple therapy. Conclusions: The current studies regarding salvage regimens are highly heterogeneous. Bismuth quadruple therapy and levofloxacin triple therapy may be a reliable option after failure of standard triple therapy, but the regional profile of antibiotic resistance should be considered. Further studies are needed for salvage regimens after failure of non-bismuth or bismuth quadruple therapy.

Silicon Nitride Cantilever Arrays Integrated with Si Heater and Piezoelectric Sensors for SPM Data Storage Applications

Hyo-Jin Nam,SeongSoo Jang,Young-Sik Kim,Caroline Sunyong Lee,Won-Hyeog Jin,Il-Joo Cho,Jong-Uk Bu 대한전자공학회 2005 Journal of semiconductor technology and science Vol.5 No.1

Silicon nitride cantilevers integrated with silicon heaters and piezoelectric sensors were developed for the scanning probe microscope (SPM) based data storage application. These nitride cantilevers are expected to have better mechanical stability and uniformity of initial bending than the previously developed silicon cantilevers. Data bits of 40 nm in diameter were recorded on PMMA film and the sensitivity of the piezoelectric sensor was 0.615 fC/nm, meaning that indentations less than 20 nm in depth can be detected. For high speed operation, 128×128 cantilever array was developed.