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Assessment of In Vitro Assay System for Thyroid HormoneDisruptors Using Rat Pituitary GH3 Cells
Hee Jin Kim1,Hae Young Park1,Jeonga Kim1,Il Hyun Kang2,Tae Sung Kim2,Soon Young Han2,Tae Seok Kang2,Kui Lea Park2,Hyung Sik Kim1 한국독성학회 2006 Toxicological Research Vol.22 No.4
The development of in vitro assays has been recommended to screening and test-ing the potential endocrine disruptors (EDs). These assay systems focus only on identifying thethe thyroid hormone (TH) disruptors. The aim of this study was to evaluate a test system to detectTH disruptors using rat pituitary tumor GH3 cells. The test system is based on the TH-dependentincrease in growth rate. As expected, L-3,5,3-triiodothyronine (T3) markedly induced a morphologicalchange in GH 3 cells from flattened fibroblastic types to rounded or spindle-shaped types. T3 stimu-lated GH3 cell growth in a dose-dependent manner with the maximum growth-stimulating effect9 M. In addition, T3 increased the release of growth hor-mone and prolactin into the medium of the GH3 cells culture. Using this assay system, the TH-dis-rupting activities of bisphenol A (BPA) and its related compounds were examined. BPA,dimethylbisphenol A (DMBPA), and TCI-EP significantly enhanced the growth of GH3 cells in therange of 1 × 10-5M to 1 × 10-6M concentrations. In conclusion, this in vitro assay system might bestandardization before it can be used as a broad-based screening tool.
Kim, Jeonga,Kim, Kyeong Rak,Hong, Yeongran,Choi, Sunyoung,Yavuz, Cafer T.,Kim, Jin Woong,Nam, Yoon Sung American Chemical Society 2019 ACS APPLIED MATERIALS & INTERFACES Vol.11 No.24
<P>Metal recovery from electronic waste and industrial wastewater has attracted increasing attention to recycle precious metals and inhibit the emission of hazardous heavy metals. However, the selective recovery of precious metals with a large quantity is still very challenging because wastewater contains a variety of different cations while precious metal ions are relatively scarce. Here, we introduce a simple method to selectively increase the adsorption of gold ions using tannin-coated porous polymer microspheres through photochemical reduction. Mesoporous poly(ethylene glycol dimethacrylate-<I>co</I>-acrylonitrile) microspheres with an average pore diameter of 13.8 nm were synthesized and used as an adsorbent matrix. Tannic acid (TA) was deposited onto the internal pores of the polymer matrix by simple immersion in an aqueous milieu. TA coatings increased the maximum number of adsorbed gold ions by 1.3 times because of the well-known metal ion chelation of TA. Under light illumination, the maximum number of adsorbed gold ions dramatically increased by 6.1 times. We examined two distinct mechanisms presumably involved in the enhanced adsorption: the photooxidation of TA and plasmon-induced hot electrons. Moreover, TA-coated microspheres exhibited remarkable selectivity for gold ions among competing metal ions commonly found in waste resources. This work suggests that the photochemically activated TA can serve as an excellent adsorbent for the selective and efficient recovery of gold ions from wastewater.</P> [FIG OMISSION]</BR>
Assessment of In Vitro Assay System for Thyroid Hormone Disruptors Using Rat Pituitary GH₃ Cells
Hee Jin Kim,Hae Young Park,Jeonga Kim,Il Hyun Kang,Tae Sung Kim,Soon Young Han,Tae Seok Kang,Kui Lea Park,Hyung Sik Kim 한국독성학회 2006 Toxicological Research Vol.22 No.4
The development of in vitro assays has been recommended to screening and testing the potential endocrine disruptors (EDs). These assay systems focus only on identifying the estrogenic or antiestrogenic activity of EDs, whereas a few studies have been carried out to screen the thyroid hormone (TH) disruptors. The aim of this study was to evaluate a test system to detect TH disruptors using rat pituitary tumor GH₃ cells. The test system is based on the TH-dependent increase in growth rate. As expected, L-3,5,3-triiodothyronine (T₃) markedly induced a morphological change in GH₃ cells from flattened fibroblastic types to rounded or spindle-shaped types. T₃ stimulated GH₃ cell growth in a dose-dependent manner with the maximum growth-stimulating effect being observed at a concentration 1 × 10? M. In addition, T₃ increased the release of growth hormone and prolactin into the medium of the GH₃ cells culture. Using this assay system, the TH-disrupting activities of bisphenol A (BPA) and its related compounds were examined. BPA, dimethylbisphenol A (DMBPA), and TCI-EP significantly enhanced the growth of GH₃ cells in the range of 1 × 10??M to 1 × 10?? M concentrations. In conclusion, this in vitro assay system might be useful for identifying potential TH disruptors. However, this method will require further evaluation and standardization before it can be used as a broad-based screening tool.
제빵용 우리밀과 수입밀 원맥의 이화학, 미생물, 항산화 특성 비교
곽한섭,김미정,허정애,김민정,심재원,김의웅,김훈,김상숙,Kwak, Han Sub,Kim, Mi Jeong,Heo, JeongAe,Kim, Min Jung,Shim, Jaewon,Kim, Oui-Woung,Kim, Hoon,Kim, Sang Sook 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.1
The objective of this study was to compare physicochemical, microbial, and antioxidant properties of domestic and imported wheat kernels for bread making. Two domestic (JK1, 2) and three imported (ND, DNS, and CWRS) kernels were compared. Domestic kernels had higher moisture contents, and lower ash and protein contents (p<0.05). In grain characteristics, JK1 had 13.62% of damaged kernels, which was the highest among the samples (p<0.05). JK2 was similar to imported kernels in the ratio of sound kernels, foreign materials, and damaged kernels. Kernel size of JK1, 2 was larger than the imported kernels; therefore, kernels area and perimeter were higher by the image analyzer. Domestic kernels hid lower total aerobic counts the imported kernels (p<0.05). Domestic kernels and DNS had no yeast, while NS and CWRS had yeast in kernels. DNS (3.08 mg gallic acid equivalent (GAE)/g) had the highest total polyphenol content (TPC), followed by JK1 (2.81 mg GAE/g). JK2 had the lowest amount of TPC as 2.26 mg GAE/g. Total flavonoid content (TFC) was the highest in DNS as 0.44 mg catechin equivalent (CE)/g and JK2 was the lowest as 0.12 mg CE/g. Domestic wheat kernels had lower protein content and lightness than the imported wheat kernels so that flour from domestic wheat kernels may have lower quality for baking.
A Study of Complex Beginnings Used by Korean EFL Learners in Two different writing genres
Jeonga Kim,Chul Ju Uhm 한국언어과학회 2020 한국언어과학회 학술대회 Vol.2020 No.10
The aim of this study is to identify the differences of complex beginnings in two different writing genres, argumentative essays and Introduction section of research proposals, written by advanced Korean EFL learners in an ESP writing class. To do this, 35 first draft of argumentative essays and 35 first draft of research proposals by 35 students were analyzed in terms of quality and quantity of the two writing genres. Of a total of 862 sentences in 13,945 words of the 35 first drafts of argumentative writing, 47.3% (408 sentences) of them included adverbials in sentence-initial (SI) position. In case of a total of 411 sentences in 8,577 words of the 35 first drafts of Introduction section of a research proposal, adverbials were found 46.7% of the sentences in SI position. Of the adverbials in SI positions, 5.6% and 5.1% respectively were complex beginnings composed of two adverbials. In order to identify complex beginnings composed of two adverbials in SI position, frequency and preference of conjunctive adverbials suggested in taxonomy types by Quirk, Greenbaum, Leech, and Svartvik (1985) were investigated. In addition, five types of complex beginnings such as stepwise orientations, compound orientation, grounded orientations, complex orientations and composite orientations suggested by Smits (2002) were distinguished. The findings will shed light on the use of adverbials by Korean EFL learners to develop coherent and cohesive relationship among sentences and maintain them throughout the contexts in two different genres.
Kim, Hyun-Joo,Cha, Gil Sun,Joo, Ji-Young,Lee, Juyoun,Kim, Sung-Jo,Lee, Jeongae,Park, So Youn,Choi, Jeomil Korean Academy of Periodontology 2017 Journal of Periodontal & Implant Science Vol.47 No.5
Purpose: Beyond the limited scope of non-specific polyclonal regulatory T cell (Treg)-based immunotherapy, which depends largely on serendipity, the present study explored a target Treg subset appropriate for the delivery of a novel epitope spreader Pep19 antigen as part of a sophisticated form of immunotherapy with defined antigen specificity that induces immune tolerance. Methods: Human polyclonal $CD4^+CD25^+CD127^{lo-}$ Tregs (127-Tregs) and $na\ddot{i}ve$ $CD4^+CD25^+CD45RA^+$ Tregs (45RA-Tregs) were isolated and were stimulated with target peptide 19 (Pep19)-pulsed dendritic cells in a tolerogenic milieu followed by ex vivo expansion. Low-dose interleukin-2 (IL-2) and rapamycin were added to selectively exclude the outgrowth of contaminating effector T cells (Teffs). The following parameters were investigated in the expanded antigen-specific Tregs: the distinct expression of the immunosuppressive Treg marker Foxp3, epigenetic stability (demethylation in the Treg-specific demethylated region), the suppression of Teffs, expression of the homing receptors CD62L/CCR7, and CD95L-mediated apoptosis. The expanded Tregs were adoptively transferred into an $NOD/scid/IL-2R{\gamma}^{-/-}$ mouse model of collagen-induced arthritis. Results: Epitope-spreader Pep19 targeting by 45RA-Tregs led to an outstanding in vitro suppressive T cell fate characterized by robust ex vivo expansion, the salient expression of Foxp3, high epigenetic stability, enhanced T cell suppression, modest expression of CD62L/CCR7, and higher resistance to CD95L-mediated apoptosis. After adoptive transfer, the distinct fate of these T cells demonstrated a potent in vivo immunotherapeutic capability, as indicated by the complete elimination of footpad swelling, prolonged survival, minimal histopathological changes, and preferential localization of $CD4^+CD25^+$ Tregs at the articular joints in a mechanistic and orchestrated way. Conclusions: We propose human $na\ddot{i}ve$ $CD4^+CD25^+CD45RA^+$ Tregs and the epitope spreader Pep19 as cellular and molecular targets for a novel antigen-specific Treg-based vaccination against collagen-induced arthritis.