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鼻Polyp組織에서 核酸分解酵素 活性度의 變動에 對한 硏究
김선곤,이형석,송기준,안경성,고재경 한양대학교 의과대학 1986 한양의대 학술지 Vol.6 No.1
In order to investigate whether activities of enzymes involved in nucleic acid metabolism were changed in nasal polyp tissues, enzyme levels of typical nucleases, ribonuclease (RNase) and deoxyribonuclease (DNase) were determined in nasal polyp tissues and were compared with those in normal control tissues. Also measured were concentrations of DNA, RNA and proteins in nasal polyp tissues, and results were as follows: 1) Activities of RNases were significantly higher in nasal polyp tissues than in normal control tissues; neutral RNase activity was increased by 92% and acid RNase by 62%. The positive rate of the enzyme activity as a marker for nasal polyp was observed to be high (46%) in neutral RNase and low (18%) in acid RNase. 2) While activities of RNases were significantly increased, activities of DNases (neutral, acid and alkaline DNases) remained unchanged, and the positive rate of DNase activities as a marker for nasal polyp was also found to be low. 3) Concentrations of DNA, RNA and proteins in nasal polyp tissues were increased by 62%, 46% and 89%, respectively, as compared with those in normal control tissues. The positive rates of nucleic acid and protein levels as a marker for nasal polyp were obtained to be high (46% each) in DNA and protein and low (18%) in RNA. 4) Neutral RNases in nasal polyp tissues were isolated in four peaks by DEAE-cellulose column chromatography. The neutral RNases isolated from nasal polyp tissues exhibited the highest activity against polycytidylate (poly C) and virtually no activity was observed against purine polyribonucleotides, polyadenylate (poly A) and polyguanylate (poly G). The RNase activity was observed to be higher with RNA as substrate than with polyuridylate (poly U). 5) The pattern of nasal polyp tissue proteins separated by polyacrylamide gel electrophoresis appeared to be different from that normal nasal tissue proteins. It was, however, unclear whether proteins specific to nasal polyp was present in the nasal polyp tissues. The results indicated that concentrations of nucleic acids and proteins and RNase activity used as a marker for tissue proliferation and tumor were significantly increased in nasal polyp tissues, but DNase activity known to be involved in development and maintenance of tumors remained unchanged. This may show the one aspect of difference between nasal polyp characterized by tissue proliferation and tumor tissues.
Efficient Polling Scheduler for IEEE 802.11 WLAN
Kim, Tae-Kon,Lee, Hyung-Keun,Koh, Jin-Hwan Institute of Korean Electrical and Electronics Eng 2007 전기전자학회논문지 Vol.11 No.4
Although the Distributed Coordination Function is the fundamental access protocol of IEEE 802.11, it cannot meet the Quality of Service (QoS) requirements in general. So, the Point Coordinate Function is provided to support QoS related services. However, it has inherent problems. Access point (AP) has no knowledge of the queue status and instantaneous channel condition of stations in the system. In this paper we propose an efficient and versatile polling scheduler that shows excellent throughput and fairness performance. Comparison with well known polling schemes is provided through computer simulation under various channel situations including error prone environments.
대장균에서의 인간 Interferon - γ의 cDNA 크로닝 및 발현
김광수,현형환,유무영,고형곤,권병세,주종광,문홍모 생화학분자생물학회 1991 BMB Reports Vol.19 No.3
A λ gt10 cDNA library constructed with poly A^+ mRNA from human peripheral blood lymphocytes was screened by a synthetic oligonucleotide having IFN-γ sequence. One of the clones (pBK04) which hybridized to the oligomer was confirmed to have the whole IFN-γ coding sequence. The cDNA insert was used to express IFN-γ in E. coli under the control of lpp promoter employing pINTIIIA3 vector. Ce115 harboring the recombinant plasmid (pKS γ 3B) expressed high level of IFN-γ assayed by antiviral activity. The molecule was also recognized by monoclonal antibody specific to a natural IFN-γ. An expression vector with high copy number (pHK-3) was constructed and was successfully used for the improvement of the expression of IFN-γ
Nucleotide Sequence of cDNA of the Complete Genome of the Tobacco Mosaic Virus Isolated in Korea
Lee, Se Yong,Koh, Hyung Kon,Song, Eun Kyung Gyeongsang National University 1991 Plant molecular biology and biotechnology research Vol.1991 No.
The cDNA of genomic RNA from a Tobacco mosaic virus (TMV) strain isolated in Suwon has been synthesized and its sequence was analyzed. Three oligonucleotide primers have been used to generate a cDNA library covering the entire TMV RNA sequence and the complete cDNA sequence of the TMV genomic RNA was reconstructed from the cDNA library. The size of the full-length cDNA was 6,395. Restriction map of the cDNA was very similar, but not identical, to that of TMV vulgare strain. Total nucleotide sequence of the cDNA was determined and showed more than 99% homology to the reported nucleotide sequence of TMV vulgare.