Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips

김나경(Na-Kyeong Kim),이효정(Hyo-Jeong Lee),김상민(Sang-Min Kim),정래동(Rae-Dong Jeong) 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.2

Barley yellow dwarf virus (BYDV) has been a major viral pathogen causing significant losses of cereal crops including oats worldwide. It spreads naturally through aphids, and a rapid, specific, and reliable diagnostic method is imperative for disease monitoring and management. Here, we established a rapid and reliable method for isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with a lateral flow strips (LFS) assay for the detection of BYDV-infected oat samples based on the conserved sequences of the BYDV coat protein gene. Specific primers and a probe for RT-RPA reacted and optimally incubated at 42oC for 10 min, and the end-labeled amplification products were visualized on LFS within 10 min. The RT-RPA-LFS assay showed no cross-reactivity with other major cereal viruses, including barley mild mosaic virus, barley yellow mosaic virus, and rice black streaked dwarf virus, indicating high specificity of the assay. The sensitivity of the RT-RPA-LFS assay was similar to that of reverse transcription polymerase chain reaction, and it was successfully validated to detect BYDV in oat samples from six different regions and in individual aphids. These results confirm the outstanding potential of the RT-RPA-LFS assay for rapid detection of BYDV.

Zn-Fe 합금 도금강판의 점용접성에 관한 연구

민준기,신재경,안효영,서수정,서창제 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.1

Spot welding is widely used in automobile assembly lines. Electroplating of Zn-Fe on steel sheets is increasingly used in assembly lines, because of its good spot weldability, formability and corrosion resistance. In this study, to evaluate spot weldability, tensile shear strength and cross tensile strength of steel sheets electroplated with Zn and Fe up to 0.7mm thickness were investigated, and these mechanical properties were compared with those of steel sheets electroplated with Zn only. The thickness of electroplated steel sheets was measured with SEM. The following conclusions were obtained. (1) In spot welding, the weldability lobe was getting wider and directed toward low currency direction, in steel sheets electroplated with Zn-Fe compared with those electroplated with Zn only. (2) Electrode force and welding time obviously affected the ultimate welding strength, and the range representing constant ultimate welding strength was observed. (3) When Zn-Fe were electroplated on steel sheets, the ductility ratio was increased in optimal welding condition, indicating that good weldability was obtained. (4) Around 8.5KA where optimal strength existed, by spot welding, the amount of the electroplated layer was observed to be reduced by approximately 15% in thickness.

Effect of Forging Type on the Deformation Heterogeneities in Multi‑Axial Diagonal Forged AA1100

Min‑Seong Kim,Sang‑Chul Kwon,Sun‑Tae Kim,Seong Lee,Hyo‑Tae Jeong,Shi‑Hoon Choi 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.3

The present study investigated the effects that different types of forging exert on the deformation heterogeneities developedin AA1100 during multi-axial diagonal forging. To measure the deformation heterogeneities of deformed workpieces, thevalues for hardness and Kernel average misorientation were measured at the center section following each forging process. Type-D forging that consists of diagonal forging and return-diagonal forging was relatively advantageous compared withType-P forging that includes plane forging and return-plane forging for minimizing the non-uniformity of deformationdeveloped in workpieces. The effective strain developed in a workpiece during the 2 types of forging was simulated using3-D FEA. FEA revealed that the positions and degrees of occurrence for soft and hard-zones in workpieces vary greatlydepending on the forging type. Type-D forging was relatively advantageous compared with Type-P forging for minimizingthe non-uniformity of effective strain developed in workpieces.

전북지방 젖소의 유방염 검색

조정곤,김종면,최민순,채효석,강명대,송희종 全北大學校 附設 畜産開發硏究所 1987 畜産開發硏究報告 Vol.1 No.-

A total of 444 quarters milk samples of 111 dairy cattle in Chonbuk area were examined for infection rete and causative agents of mastitis by "the laboratory procedures used in the Connecticut Mastitis Control Program (CMCP) and California mastitis Test (CMT)". The results obtained were as follows: 1. Of 444 samples, 39.2% had positive CMCP value and 24.4% positive CMT reaction. 2. One hendred and seventy four (39.2%) from 82 cattle (73.9%) were found to infected with mastitis. It was found that 8(1.8%0 of the infected quarters were clinical mastitis and all of the rest were subclinical mastitis. 3. The main causative agents were found to Staphylococcus aureus (63 quarters), Streptococcus agalactiae (56 quarters), non-Str. agalactiae streptococci (54 querters), Escherichia coli (19 quaters), the actual total number of quarters detected as mastitis would be 174, as 18 of these quarters showed multiple infection with two different organisms. 4. Having examined the number of infection quarter per head of mastitis in dairy cattle sampling, major finding were that the average number of infection quarters to total dairy cattle exemined was 1.57 quarters, and that of the infected cattle was 2.1 quarters. Infected quarters in cattle with 1, 2, 3 and 4 were noted 27(32.9%), 28(34.1%), 17(20.7%), and 10(12.3%), respectively.

심근경색 환자에서의 헤파린 사용시 장요근 혈종과 연관된 후복막강내 자발적 출혈 1예

김효정,김도연,황민규,조홍근 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.10

乳房炎 乳汁에서 分離된 原因菌의 抗菌劑 感受性

崔民淳,金鍾冕,趙正坤,蔡孝錫,姜明大,宋熹鍾 全北大學校 附設 畜産開發硏究所 1987 畜産開發硏究報告 Vol.1 No.-

A total of 189 strains (Streptococcus agalactiae and non-Streptococcus agalactiae 110, Staphylococcus aureus 63, and E. coli 16) isolated from bovine mastitie milk samples by "The Connecticut Mastitis Control Program" in dairy herds were examined for drug susceptibility to 8 drugs and analysed the rate of multiple resistant patterns. 1. All of the isolates were resistant to antimicrobial agents such as penicillin (PC), streptomycin (SM), kanamycin(KM), carbenicillin (CA), lincomycin (LM), gentamicin (GM), and tetracycline (TC), singly or multiply. The isolates were more susceptible to CA (84.1%),PC (78.9%), and SM (78.4%) than the the other drugs such as GM (51.8%), KM (48.7%), TC (19.6%) and LM (19.0%) in order. 2. The multiple resistant isolates (96.3%) were more than those of singly resistant isolates (3.7%) and 58 different drug resistant patterns were observed. Among 7 singly resistant isolates, CP was the commonest. While among 182 multiple resistant isolates, CP-KM-LM-TC-GM, CP-LM-TC, CP-LM-TC-GM, PC-CP-SM-LM-TC-GM, CP-KM-LM-TC, and CP-LM patterns were frequently encounted. 3. The strains of streptococcus spp. were more susceptible to CA (90.1%) and SM (82.9%) than the other drugs such as KM (43.2%), GM (38.7%), LM (13.5%), CP (10.8%) and TC (10.8%). Among them CP-KM-LM-TC-CM pattern was frequently encounted. 4. The strains of Sta. aureus were more susceptible to PC (74.6%),CA (69.8%), SM (65.1%) and GM (63.5%) than the other drugs such as KM (46.0%), CP (22.2%), TC (15.9%) and LM (9.5%). Among them CP-LM-TC pattern was commonly encounted. 5. The strains of E. coli were more susceptible to CA (100%) and KM (75.0%) tnan TC (9.5%). But the other drugs such as PC, SM, GM, CP and LM were not. Among them PC-CP-SM-LM-TC-GM pattern was commonly encounted.

천추 갈색종양(Brown tumor)으로 발현된 부갑상선 기능항진증 1예

홍우정,구본정,이재민,한상우,이효진,나소영,노흥규,김영건,송민호 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

부갑상선 기능항진증에 의한 골병변으로 갈색종양이 있다. 갈색종양은 주로 장골을 침범하고 척추골에서의 발생은 매우 드물다. 국내의 보고에서 갈색종양의 장골 침범은 있었으나, 척추골에서의 발생은 보고된바 없다 .저자들은 부갑상선 기능항진증으로 인하여 척추골의 천추에 발생된 갈색종양 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. The bony involvement in primary hyperparathyroidism present as browm tumor or osteitis fibrosa cystica and lead to pathologic fracture. Brown tumor commonly involve long bone such as femur, tibia, fibula, humerus, and radius. Brown tumor of axial skeleton is a rare entity. Previously reported case that involve axial skeleton still not present. We report a 47-year-old male patient that he has a hyperparathyroidism presenting as brown tumor involving sacrum.

Methylphenidate 투약에 따른 주의력결핍 과잉운동장애 아동의 정량화 뇌파의 변화

이병대,정성훈,이만기,장수민,백지령,임효덕 대한생물치료정신의학회 2002 생물치료정신의학 Vol.8 No.1

HMG-CoA 환원효소 억제제에 의한 ICAM-1 유전자의 발현조절

김현진,정효균,홍우정,김군순,조영석,김도희,채수흥,구본정,송민호,노흥규,김영건 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : ICAM-1 act as one of major adhesion molecules in the atherosclerotic lesion. ICAM-1 expression is mainly regulated at the level of transcription and depend on IFN-γ signal transduction pathway in which the STAT1 transcrption factor is a critical intermediate. IFN-γreceptor not only initiates tyrosine 701 phosphorylation of STAT1 by Jak1 and Jak2, but also phosphorylates serine 727 through the activation of Raf-1/MAP kinases. HMG-CoA reductase inhibitors have anti-atherosclertic effects, beyond normalization of hypercholesterolemia, by directly acting on endothelial cells, macrophages and vascular smooth muscle cells. HMG-CoA reductase inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibition posttranslational farnesylation and geranyl-geranylation processing of small GTP-binding preoteins and inhibition of normal signaling activities. Method : We made several 5'-deletion constructs of rat ICAM-1 promoter and analyzed the promoter activities by measuring the luciferase activity after transfection into ECV304 cells and smooth muscle cells. We checked the level of total and phosphorylated STAT1 protein by immunoblot analysis using specific antibodies. Results : Lovastatin inhibits IFN-γ-induced ICAM-1 gene expression in the ECV304cell. The cells pretreated with PD98059, MEKK inhibitor showed significantly low ICAM-1 RNA induction with IFN-γ stimulatio. IFN-γ induced phosphorylation of tyrosine 701 was not significantly changed by the pretreatment of lovastatin. But lovastatin suppresses IFN-γ-induced phosphorylation of ERK1/ERK2 which are responsible for the seine 727 phosphorylation in STAT1. Conclusion : We showed that HMG-CoA reductase inhibitors, lovastatin, suppresses IFN-γ mediated ICAM-1 gene expression through the inhibition of transcription. HMG-CoA reductase inhibitor suppresses IFN-γ-induced phosphorylation of serine 727 in STAT1 through the modulation of MAP kinases.

마이크로 그루브 가공의 특성 평가

권동희,박진효,김정석,김병민,정융호,강명창 한국공작기계학회 2007 한국공작기계학회 추계학술대회논문집 Vol.2007 No.-

As demands for micro parts are on the increase in recent industries such as aerospace, biomedical, electronics, environment, information technology and display, the need for manufacturing such parts is also increasing. Major methods of manufacturing micro parts are based on semiconductor process technology such as lithography and etching and non-traditional machining using lasers, ultrasonic, ion-beams or electrical discharge. However, these methods require lots of time, workpiece materials are limited, and such methods are only applicable to the machining of two-dimensional shapes. Therefore, mechanical machining methods are required for the manufacturing of micro parts with complex shapes. This paper presents an investigation on the characteristics for micro groove machining by using the ultrahigh-speed air turbine spindle, and verifies the properties of mechanical method in fabricating molds for micro barrier ribs. As a result, the optimal machining condition could be found from the investigation of surface roughness according to the kinds of burr shapes in the fabricating of iron mold by using micro endmill.