Anti-Inflammatory Effect of By-Products from<i>Haliotis discus hannai</i>in RAW 264.7 Cells

Rho, Ho-Seok,Kim, Hyang,Kim, Jung-Ae,Karadeniz, Fatih,Ahn, Byul-Nim,Nam, Ki-Ho,Seo, Youngwan,Kong, Chang-Suk Hindawi Limited 2015 Journal of chemistry Vol.2015 No.-

<P>Several reports promoted the potential of shellfish due to their ability to act as antioxidant, anti-inflammatory, and antimicrobial agents. Pacific abalone,<I>Haliotis discus hannai</I>viscera is, reported to possess bioactivities such as antioxidative stress and anti-inflammatory. In this study, anti-inflammatory potential of mucus-secreting glands from shell-shucking waste of<I>H. discus hannai</I>was evaluated using RAW 264.7 mouse macrophage cell model. Results indicated that presence of<I>H. discus hannai</I>mucosubstance by-products (AM) significantly lowered the nitric oxide (NO) production along the expressional suppression of inflammatory mediators such as cytokines TNF-<I>α</I>, IL-1<I>β</I>, and IL-6 and enzymes iNOS and COX-2. Also, AM was shown to increase expression of anti-inflammatory response mediator HO-1. Presence of AM also scavenged the free radicals<I>in vitro</I>. In conclusion, by-products of<I>H. discus hannai</I>are suggested to possess notable anti-inflammatory potential which promotes the possibility of utilization as functional food ingredient.</P>

Interleukin-2를 이용한 진행성 위장관암의 비장을 경유한 자가 LAK Cell의 면역학적 반응

안우섭,강중신,김인호,전석길,손수상 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.3

To elucidate the anticancer effect by observing the effect of LAK cells after infusion of IL-2 through splenic artery for the advanced gastrointestinal cancer, randomized study was performed for the 8 patients who had liver metastasis from gastic and rectal cancer. 4 patients were performed subtotal gastrectomy for stomach cancer and the others were done abdominoperineal resections for rectal cancer. The liver metastasis were noted all of the cases after surgery between October 1989 and December 1992 at the Department of Surgery, Keimyung University School of Medicine. After confirmation of the hepatic metastasis from gastorintestinal cancer, we performed selective splenic arterial infusin of IL-2 with the dosage of 20,000 units/kg B.W. We checked the immune function pre-and post-IL2 infusion therapy, such as total lymphocyte count, T-cell percentage and CMI multitest with blood count and hepatorenal function. There was no difference between the pre-and post IL-2 infusion in the hemoglobin, serum protein, albumin, BUN and creatinin. The total lymphocyte counts of pre-and post IL-2 infusion were 1641.2 and 1727.4, respectively. The T cell percentage of pre-and infusion were 62.51% and 64.27%, respectively and had the tendency to increase in comparison with pre-IL-2 infusion, but there were not stastical differences. The results of CML muiltitest of pre-and post IL-2 infusion were not definite differences. From the above results, it could be suggested that LAK cell after infusion of IL-2 via selective splenc artery had the slightly positive effect of the immune function of advanced gastrointestinal cancer patient, with hepatic metastasis.

STAFF와 SECRETARY에 대한 사회과학적 재조명 : 직업교육으로서의 비서학 Secretarial Studies as a Vocational Education

안호석 이화여자대학교 법정대학 비서학과 1988 비서학연구 Vol.- No.8

양측 쇄골하동맥 및 양측 신동맥 협착을 동반한 Takayasu 동맥염 1예

임선호,임수빈,오석규,이재홍,정진원,박양규,박옥규,소병준,노병석 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.8

소양호에서 세균수와 세균 체적의 계절적 수심별 변화

안태석,최승익,변명섭,박호완 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-

To define the ecological roles of bacteria in Lake Soynng, the depth profiles of bacterial numbers and their biovolume were estimated temporally. In August, 1992, the bacterial numbers and biovolume were ranged from 12×10^(5) to 28×10^(5) cells ml^(-1) and from 0.4 to 1.0 ㎛³ cell^(-1), respectively. At 30m depth, the biovolume was highest as 1.0㎛³ cell^(-1) while the numbers were not changed. These large bacteria might be allochthonous bacteria originated from terrestrial during the heavy rain. In October, 1882, the bacterial numbers were ranged from 6×10^(5) to 17×10^(5) cells ml³ , and biovolumes at epilimnion were about 0.5 ㎛³ cell^(-1), while at metalimnion and hypolimnion, larger than 0.9 ㎛³ cell^(-1). In this time, zooplankton grazing pressure and nutrients release from sediment might affect the bacterial numbers and biovolume. In April, 1993, when Asterionella and Fragifaria were dominant species of phytoplankton only in epilimnion, the bacterial numbers were about 20×10^(5) cells ml^(-1), and biovolumes were about 0.8㎛³ cell^(-1). While at other depths, the numbers and biovolumes were smaller. These results suggest that the bacterial numbers were dynamically changed with season, while their mean cell volume were commonly stable as 0.5㎛³ cell^(-1). But by the allochthonous bacteria, organic materials released by phytoplnnkton and grazing pressure by zooplankton might increase the biovolume.

외상성 횡격막 손상

안성국,이상목,이기형,고석환,김용호,박호철,고영관,조규석 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Fifty two cases of traumatic diaphragmatic injuries that we have experienced from Jan. 1973 to Oct. 1994 were evaluated. The following results were obtained. The age of the patient was ranged from 1 to 74 years. Male was 38 and female was 14 in number with a ratio of 2.7 : 1. The traumatic diaphragmatic injuries were due to blunt trauma in 35 cases(motor vehicle accident 25, fall down 8, press 1, kick by fight 1) and penetrating trauma in 17 cases(stab wound 15, shot wound 1, explosion 1). In the blunt trauma, the preoperative diagnosis of the diaphragmatic injury was possible in 25 out of 35 cases(71%) and in the penetrating trauma, 15 out of 17 cases(88%). In the blunt trauma, the repture site was located in the left in 22 cases(63%) and in the right in 13 cases(31%). In the penetrating trauma, the rupture site was located in the left in 9 cases(53%) and in the right in 8 cases(47%). In the blunt trauma, 20 cases(63%) were treated within 24 hours and in the penetrating, 15 cases(88%) within 24 hours. In the blunt trauma, the herniated organs into the thorax were stomach(7), omentum(6), spleen(6), liver(5), colon(4), small bowel(2) and in the penetrating, stomach(7), colon(6), omentum(3), liver(2), and spleen(1) were herniated. Injury severity score(ISS) of 35-blunt trauma ranged from 11 to 66 with mean value of 30.6. Mean ISS of survivors and nonsurvivors was 27.6 and 52.7 respectively. The diaphragmatic repair of 49 cases was performed with thoracic approach in 23 cases, thoracoabdominal approach in 7 cases and abdominal approach in 19 cases, and 3 cases were not operated. The postoperative complication and mortality were developed in 16 out of 49 cases(33%) and in 5 cases(9.6%) respectively, and the causes of death were hypovolemic shock(1), combined head injury(2), asphyxia(1), and pulomnary edema and renal failure(1). In conclusion, the injuries of the diaphragm should be suspected in all patients with severe blunt trauma or penetrating injuries at thorax and upper abdominal area near the diaphragm. All of the cases had associated injury and most of deaths were related to the severity of associated injuries.

방사선조사가 흰쥐 소뇌겉질의 미세구조에 미치는 영향

안의태,고정식,박경호,유석준 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.6

應力과 캐비테이션 下에서의 應力腐蝕 擧動에 관한 硏究

황재호,임우재,안석환 釜山水産大學校 1994 釜山水産大學 硏究報告 Vol.34 No.1

In this paper, stress corrosion cracking behavior, and stress corrosion cracking behavior under cavitation-erosion of water injection were investigated. Main result obtained were as follows 1) The weight loss of stress corrosion under stress is less susceptive than that of stress corrosion under stress and cavitation environment. 2) Cracking growth rate of stress corrosion under stress and cavitation is faster than that of stress corrosion under stress. 3) Corrosion potential of stress corrosion under stress and cavitation is less noble than that of stress corrosion under stress. And corrosion current density under stress and cavitation is higher than that of stress corrosion under stress.

東洋系 Cymbidium屬 種子의 無菌發芽와 幼苗의 增殖體系 確立

姜鎬宗,安相烈,蔡潤錫 진주산업대학교 1990 論文集 Vol.28 No.-

동양계 Symbidium 속인 산천보세란 종자의 무균발아와 유묘증식을 위하여 근경형성을 위한 초기배양방법의 확립, 근경 및 유묘의 증식에 미치는 배지 및 Hormone제의 영향에 관한 실험을 수행한 결과를 요약하면 다음과 같다. 산천보세란 종자발아는 H_3P_4+NAA 0.1㎎/ℓ, Kinetine 0.01㎎/ℓ첨가배지에서 가장 양호하였으며 발아에 소요된 일수도 210일로서 가장 짧았다. 종자발아에서 얻은 근경의 재증식은 H_3P_4배지에 NAA 1㎎/ℓ, Kinetine 1.0~3.0㎎/ℓ첨가한 배지에서 양호하였으며, 유묘의 증식과 생장에 있어서는 H_3P_4+α-as-paratic acid 0.5㎎/ℓ, NAA 1.0㎎/ℓ, BA 3㎎/ℓ첨가 배지에서 가장 양호하였다. 지금까지의 실험을 종합하여 볼때 무균종자를 파종해서 발아시킨 후, 근경을 급속으로 증식시키고 H_3P_4+NAA 1.0㎎/ℓ함유배지에 BA 3㎎/ℓ을 첨가시킨 후 치상하여 multiple shooting을 시킨 다음 동일조성의 배지에 Hormone제를 제거하고 활성탄 1.6g/ℓ을 첨가한 배지에 이식해서 생장을 도모한 후 광도를 높이고 온도를 낮추며 일장을 16시간 정도 조절하여 순화시킨뒤 폿드 이식 가능한 유묘를 대량샌산 할 수 있을것으로 판단된다. In the process of nonsymbiotic germination and seedling multiplication of Sancheunboseran seed (temperate zone ochid, cymbidium sinense Willd); to establis the early culture method for rhizome formation and to examine the effect of various media and hormone drugs upon the multiplication of rhizome and seeding were the main prupose of this experiment. The results obtained are as follows: The medium with hyponex 3g/ℓ, peptone 4g/ℓ, NAA 0.1㎎/ℓ and Kinetine 0.01㎎/ℓ added was best for the seed germination for Samcheunboseran: the days required for germination were 210- the shortest of all mcdia. The remultiplication of rhzome acquired in the seed germination was most active in the medium with hyponex 3g/ℓ, peptone 4g/ℓ and kinetin 1~3㎎/ℓ added. The seedling showed the best multiplicatin and growth in the medium with hyponex 3g/ℓ, peptone 4g/ℓ, L-asparatic acid 0.5㎎/ℓ, NAA 1.0㎎/ℓ, and BA 3㎎/ℓadded. These results recommend us to plant the aseptic seed, to multiply rhizomes rapidly after germination, to plant the rhizome in the medium with hyponex 3g/ℓ, peptone 4g/ℓ, and NAA 1㎎/ℓ added, to remove the hormone drugs from the medium after multiple shooting and add charcoal(1.6g/ℓ) to the medium, and to trnsplant seedling to the medium. Such procedure will make it possible to produce pottable seedling massively.

가시오가피의 부위별 물 추출물이 흰쥐의 혈관이완반응에 미치는 영향

姜奉錫,金亨煥,安德均,崔湖榮 대한본초학회 2001 大韓本草學會誌 Vol.16 No.2

In this study we have examined the actions of water extract of Eleutherococus senticosus (Rupr. et Maxim.) Maxim. (ESH), Stem, Root bark (RB) and Root xylem (RX) in isolated thoracic and abdominal aorta assay. To compare the vasodilation effect, put. ESH, Stem, RB and RX into them which vessels were precontracted by 5-hydroxytryptamine (5-HT). We found out the thoracic aorta segments were responded to ESH with a dose-dependent vasodilation. The 5-HT induced contraction at 10-4M were relaxed after addition of the 2.0 ㎎/㎖ ESH as 32.1% (Stem), 34.9% (RX) and 87.2% (RB). The 5-HT induced vasocontraction at 10^-4M were vasodilated such as 87.2% in thoracic aorta and 89.0% in abdominal aorta with 2.0㎎/㎖ ESH. Addition of 2.0㎎/㎖ ESH in thoracic aorta which was precontracted by the 5-HT at 10-4M were come out differently 0% (water fraction), 53.8% (butanol fraction) and 96.6% (ethyl acetate fraction). The 5-HT induced contraction at 10-4M with and without endothelium were inhibited by 87.2% and 87.8% after addition of the ESH (2.0 ㎎/㎖). In conclusion, ESH-induced relaxation in the isolated rat thoracic aorta and abdominal aorta were come out be dose-dependent relaxation, and ESH's ethyl acetate fraction was showed the potent vasodilation effect.