한방 이화주의 미백 및 피부 주름 개선 효과

이상진(Sang-Jin Lee),권이영(Yi-Young Kwon),조성원(Sung-Won Cho),권희숙(Hee-Suk Kwon),신우창(Woo-Chang Shin) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.4

본 연구에서는 한방이화주의 피부 생리기능 활성을 알아보기 위해 70% EtOH 추출물의 피부 미백, 주름 개선 및 항염증 효과를 조사하였다. HEE은 tyrosinase 활성 억제 및 tyrosine을 기질로 melanin이 형성되는 pathway에 관여하는 주요한 인자인 TRP-1과 TRP-2를 저해하는 작용 기전을 통해 피부 색소침착의 주요 원인 물질인 melanin 생합성을 농도 의존적으로 저해하는 것을 확인하였다. HEE은 피부진피 내 피부 탄력을 유지하는 elastin을 분해하는 효소인 elastase의 활성을 저해하였고, 피부의 keratinocyte가 생성분비하는 MMP-2와 MMP-9의 단백질 발현과 proteolytic 활성을 억제하여 노화에 따른 피부 주름 생성 억제할 수 있는 가능성을 확인하였다. 또한 세포 독성 없이 LPS에 의해 유도된 염증 반응을 50% 저해하는 HEE의 농도(IC50)는 24.9 μg/mL이며, 50 μg/mL 농도로 처리하였을 때 염증 반응 저해 효과가 70%로 높은 효과를 가지는 것을 확인하였다. 이상의 결과를 종합하면 HEE의 피부 미백, 주름 개선 및 항염증에 우수한 효과를 나타내고 있으므로 기능성 화장품의 주요한 소재로 이용 가치가 높을 것으로 사료된다. Extrinsic skin aging is characterized by the loss of skin tone and resilience, irregular pigmentation, and deep wrinkles. The aim of this study was to investigate the effects of Ehwa Makgeolli containing oriental herbs (Glycyrrhiza uralensis Fisch., Lycium chinense MILL., Morus alba L., and Saururus chinensis Baill) on skin whitening and wrinkling in human skin cells. We prepared Makgeolli extracts (HEE) with 70% ethanol. HEE significantly inhibited in vitro mushroom tyrosinase activity and reduced the cellular and secreted melanin content of mouse melanoma melanocytes (B16F1 cells). HEE down-regulated the protein expression of tyrosinase related protein (TRP)-1/-2, a key player in melanogenesis. Treatment with HEE in human keratinoctyes (HaCaT cells) inhibited the proteolytic activities of matrix metalloproteinase (MMP)-2/-9 in a dose-dependent manner and dramatically reduced the expression of MMP-2/-9. In addition, HEE attenuated lipopolysaccharide (LPS)- induced nitric oxide production in murine macrophages (RAW264.7 cells). These results indicate that HEE may be a great cosmeceutical ingredient for its whitening, anti-wrinkle, and anti-inflammatory effects.

후방 도달 척추 절제술을 이용한 강직성 요천추 변형의 치료

석세일,정의룡,이정희,김진혁,김성수,이지호,최원기,박용원 대한척추외과학회 2004 대한척추외과학회지 Vol.11 No.2

심한 척추변형(측만증 및 후만증)에 대한 척추 후방 절제술

석세일,김진혁,이상민,정의룡,이정희,김성수,정락용 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.1S

■ Purpose To report a technique of vertebral column resection through a single posterior approach and its first results in the treatment of severe spinal deformities. ■ Methods and materials Seventy spinal deformity patients treated by posterior vertebral column resection(PVCR) were reviewed. Minimum follow up was 2 years (2 - 3.3 years). There were 34 males and 36 females with a mean age of 27.4 years at the time of the operation. Etiologic diagnoses were adult scoliosis in 7, congenital kyphoscoliosis in 38, and post-infectious kyphosis in 25. The surgery consisted of temporary stabilization of the vertebral column with segmental pedicle screw fixation, resection of the vertebral column at the apex of the deformity via the posterior route followed by gradual deformity correction and global fusion. ■ Results The total number of resected vertebrae were 143; 76 in thoracic and 67 in lumbar. Mean operation time was 4 hours 31 minutes with average blood loss of 2333 ml. The deformity correction was 40o in the coronal plane and 5Oo in the sagiotal plane. Complications were encountered in 24 patients: 2 complete cord injuries in severe adult scoliosis and thoracic kyphosis patient who had significant preoperative cord compromise, 6 hematomas, 4 root injuries (all incomplete), 5 fixation failures, 2 infections and 5 hemopneumothoraxes. ■ Conclusions PVCR is an effective alternative for severe fixed spinal deformities. However, extreme caution must be taken in the patients with preoperative cord compromise who are the high risk of neurological complications.

분절간 척추경 나사 고정과 척추전이법을 이용한 특발성 척추 측만증의 치료

석세일,김진혁,이상민,정의룡,김성수,이정희,박준석 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2

Objective : To report the clinical result of segmental pedicle screw fixation and translation technique in surgical correction of idiopathic scoliosis. Methods and materials : Ten idiopathic scoliosis. patients treated with segmental pedicle screw fixation and translation technique were analyzed retrospectively. The mean follow-up interval was 23.8months, with a minimum of 12 months. Eighteen structural curves were fused in one double curves(King type Ⅰ), seven double thoracic curve(King type Ⅴ) and two thoracolumbar curve. Deformity correction, apical vertebral rotation(AVR), apical vertebral translation(AVT), trunk balance, shoulder balance and complications were evaluated. Result : The structural curve was corrected from 47.4±10.6° to 20.3±5.9° at 1week after surgery and 55.6±14.1° at latest follow-up. AVR was corrected from 2.06±0.4 to 0.94±0.41 at 1week after surgery and latest follow-up. AVT was improved from 25±15.7㎜ to 11.5±7.3㎜ at 1week after surgery and 12.3±7.3㎜ at latest follow-up. There were no trunk imbalance(less than 10㎜ distance from C7 plumb line), no shoulder imbalance(less than 10㎜ shoulder height difference) and no screw related complications. Conclusions : The segmental pedicle screw fixation and translation technique is an effective method in treating for idiopathic scoliosis.

토양환경지도 자료를 이용한 콩밭 토양의 화학성 비교

박지숙,이민진,정재원,김미혜,이서연,지윤미,한준호,김유학,공명석,한광현,노희명 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

It is important to interpret the soil chemical properties in order to effectively manage the farmland. This study was conducted to investigate the soil chemical properties of upland soybean field according to the soil series at 32 upland fields located in the parts of Gangwon-do. Soil sampling sites were selected by using the National Statistics DB and KSIS DB that is a soil environment information system provided by the Rural Development Administration. Soil samples were collected from 0~15 cm of top soil before seeding or transplanting, air-dried, passed through a 2 mm sieve, and analyzed for soil pH, EC, organic matter, available phosphorus (P2O5), exchangeable potassium, exchangeable calcium and exchangeable magnesium. The average chemical properties have exceeded the each nutrient optimum range level of RDA, except the organic matter in experimental soil 2015. And despite application of conventional fertilization, each soil series nutrient contents were change respectively. The results of this study suggest that conventional fertilization should be applied based on the analysis about the amount of accumulated nutrient contents in the soil.

FAF1의 도메인 특이적 항체 제조 및 검증

이윤숙,채진희,김은희 충남대학교 생물공학연구소 2009 생물공학연구지 Vol.15 No.-

한국산 배의 Polyphenol 화합물군이 쥐의 면역기능에 미치는 영향

최희진,한호석,박정혜,배종호,우희섭,안봉전,배만종,김현구,최청 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.4

This study was conducted to investigate immunofunctional activity of the polyphenol fractions isolated from Korean pear. In the experimental of Rosette forming cell, the results showed that all the polyphenol fractions enhance the cell count compared with the control group. Especially polyphenol fraction II and III showed highly significant effect on Rosette forming cell, and allergy inhibition. After antigen challenge, histamine content of blood of the polyphenol groups was lowered to near the normal group.

할로겐화 탄화수소의 흡착제거기술 Ⅱ. Pressure Swing Adsorption 공정에 의한 할로겐화 탄화수소/공기 혼합물의 분리

이태진,안희관,김윤갑,강석호 嶺南大學校 環境問題硏究所 1990 環境硏究 Vol.10 No.1

An experimental study was performed for the bulk separation of halogenated hydrocarbons and air mixture(CF₂Cl₂/air, CH₂Cl₂/air=0.1/99.9 Vol.%) in a pressure swing adsorption (PSA) process packed with various adsorbents, The PSA process cycle comprised four stes : I pressurizaton, II adsorption, III countercurrent depressurrzation, IV purge. The order of appearance of break-through curve is MS-5A > MS-13X > W-AC > S-AC for CF₂Cl₂/Air and W-AC > S-AC > MS-13X for CH₂Cl₂/Air respectively. The separation effects for the variation of adsorption pressure were investigated. In a steady state PSA cycles, high purity air from step II and CFCs-rich gas from step IV were obtained.

'광물의 감정' 멀티미디어 학습 프로그램의 개발

양은진,최석원,김희수 공주대학교 사범대학 과학교육연구소 1999 과학교육연구 Vol.30 No.1

고등학교 지구과학 '광물의 감정' 내용에 대한 컴퓨터 보조학습 프로그램이 16 bit IBM-PC 기종에서 학습할 수 있도록 한국형 저작도구(KAS)로 개발되었다. 이 프로그램은 하이퍼텍스트 및 인터럽트 기능 등을 이용하여 만들어 졌으며 학습자가 혼자서 학습할 수 있는 반복 연습형이다. 그리고 이 프로그램은 학습자와 컴퓨터와의 상호작용 효과를 고려하여 제작되었다. 'Judgememt of Minerals' Muitimedia program is developed as an helping tool for the content of high school earth science with 16 bit IBM-PC compatible computer using 'KAS(Korea Authoring System) Ver.3.0'. This program is Drill and Practice type for individual study through, hypertext, interupt functions. And the program is considered to the reciprocal action effect between learner and computer.

W(110) 표면에 CO의 흡착

이경희,유위량,한현석,부진효,이순보,곽현태 성균관대학교 기초과학연구소 1998 論文集 Vol.49 No.-

The interaction of CO with W(110) surface was investigated through LEED, TDS, and photoelectron spectroscopy using synchrotron radiation under UHV condition. After CO saturation at RT, two desorption states, called α and β , were observed at about 400 and 1150 K in thermal desorption spectra, respectively. The kinetics of 3-CO followed the first order kinetics, indicating the existence of molecular CO on W(110) surface. This is contrary to the previous results. The O 1s BE(binding energy) of CO adsorbed on W(110) surface at room temperature was 529.9 eV. On the other hand, the O 1s BE of β-CO after heating to 900 K was different from that of oxygen adsorbed W(110) surface, suggesting a different adsorption state. According to the UP valence band spectra, we observed two peaks at near -10.7 eV (4σ) and -7.0 eV (5σ+1π), indicating the molecular adsorption of CO at room temperature. Furthermore we could see the 4σ peak at the various photon energy and elevated temperatures. Comparing the energy separation, Δ(4σ-1π) , between 4σ and 1π UP peaks of chemisorbed CO, we found that an increased separation reflects an decreased C-O bond strength. Therefore on the basis of TDS and photoelectron spectroscopy, we could suggest that β state of CO on W(110) may not be dissociated and has an adsorption geometry of lying-down mode.