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Cho, I-R,Kaowinn, S,Song, J,Kim, S,Koh, S S,Kang, H-Y,Ha, N-C,Lee, K H,Jun, H-S,Chung, Y-H Nature America, Inc. 2015 Cancer gene therapy Vol.22 No.5
Although H-1 parvovirus is used as an antitumor agent, not much is known about the relationship between its specific tropism and oncolytic activity. We hypothesize that VP2, a major capsid protein of H-1 virus, determines H-1-specific tropism. To assess this, we constructed chimeric H-1 viruses expressing Kilham rat virus (KRV) capsid proteins, in their complete or partial forms. Chimeric H-1 viruses (CH1, CH2 and CH3) containing the whole KRV VP2 domain could not induce cytolysis in HeLa, A549 and Panc-1 cells. However, the other chimeric H-1 viruses (CH4 and CH5) expressing a partial KRV VP2 domain induced cytolysis. Additionally, the significant cytopathic effect caused by CH4 and CH5 infection in HeLa cells resulted from preferential viral amplification via DNA replication, RNA transcription and protein synthesis. Modeling of VP2 capsid protein showed that two variable regions (VRs) (VR0 and VR2) of H-1 VP2 protein protrude outward, because of the insertion of extra amino-acid residues, as compared with those of KRV VP2 protein. This might explain the precedence of H-1 VP2 protein over KRV in determining oncolytic activity in human cancer cells. Taking these results together, we propose that the VP2 protein of oncolytic H-1 parvovirus determines its specific tropism in human cancer cells.
패스틴^� 첨가가 단백질 분해율과 반추위 발효 및 영양소 소화율에 미치는 영향
최유지,최낙진,박성호,송재용,엄재상,고종렬,하종규 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.5
본 시험은 패스틴^R을 첨가하였을 때, in vitro 상에서 단백질 fraction과 분해율에 미치는 영향과, in vivo 상에서 반추위 성상, 미생물 군집, 암모니아태 질소 농도 및 영양소 소화율에 미치는 영향을 규명하고자 실시하였다. In vitro 실험에서는 1㎜로 분쇄된 대두박을 기질로 하여 패스틴^R(㈜은진인터내셔날)을 첨가하여 borate-phosphate buffer와 중성세제에서의 조단백질 분해율을 측정하였으며, exogenous enzyme (Streptomyces griseus 유래 protease)를 이용하여 39℃에서 0, 2, 4, 8, 12, 48 시간동안 배양 후 조단백질 분해율을 측정하였다. 반추위 발효성상과 영양소 소화율은 반추위 fistula가 부착된 평균체중 300㎏의 홀스타인 수소 4두를 이용하여 무첨가구, 패스틴^R 첨가구의 두 개 처리구에 2마리씩 4마리를 배치하여 측정하였다. Buffer Soluble Protein fraction은 패스틴^R 첨가 수준별로 차이가 없었으나, 무첨가구에 비해 패스틴^R 첨가구에서 감소하는 경향을 보였다. 단백질 분해율은 배양 0 시간대에서 4시간대까지는 처리구간 유의성이 없었지만, 12 h과 48 h에서는 패스틴^R 첨가로 시험구에서 감소되었다. 용해 단백질 분해율 “a”는 패스틴^R 시험구에서 경미하게 높은 수치를 나타내었지만, 소화 가능한 단백질 분해율 “a+b”는 패스틴^R 시험구에서 낮은 경향을 보였다. 패스틴^R 첨가로 pH와 NH_3-N 농도는 증가하는 경향이었으며 휘발성지방산, 미생물 수 및 enzyme activity는 감소하였고 영양소 소화율은 높았으나 유의적인 차이는 없었다. This study, including two in vitro experiments and an invivo experiment were conducted to evaluate effects of Passtein^R on crude protein degradability, ruminal fermentation characteristics and nutrient digestibility. In in vitro experiment protein degradability was examined using borate-phosphate buffer and neutral detergent, and using protease from Stroptomyces griseus at 39℃ for 0, 2, 4, 8, 12 and 48 h. In addition, an in vivo experiment was conducted in a switch back design and ruminal fermentation and nutrient digestibility were determined. Four ruminal-fistulated Holstein cows weighing 300㎏ in mean body weight randomly allotted to 2 treatments (control and Passtein^R supplementation). Although there was no significant difference on protein fraction between treatments, it appears that Passtein^R supplementation decreased buffer soluble protein fraction compared to control. Protein degradability was not affected by Passtein^R from 0 h to 4 h, but decreased at 12 h and 48 h compared to control. Degradation of immediately degradable fraction was higher in Passtein^R treatment, but degradation of fermentable fraction was lower in Passtein^R treatment compared to control. The pH and NH_3-N concentration tended to increase in Passtein^R treatment, but VFA production, microbial counts and enzyme activity tended to decrease in Passtein^R treatment compared to control. In addition, nutrient digestibility in the total tract tended to increase in Passtein^R treatment compared to control.
Jin, Y.C.,Kim, C.W.,Kim, Y.M.,Nizamutdinova, I.T.,Ha, Y.M.,Kim, H.J.,Seo, H.G.,Son, K.H.,Jeon, S.J.,Kang, S.S.,Kim, Y.S.,Kam, S.C.,Lee, J.H.,Chang, K.C. North-Holland ; Elsevier Science Ltd 2009 european journal of pharmacology Vol.614 No.1
The aim of the present study was to evaluate the protective effect of cryptotanshinone (CTS), one of active ingredients of Salvia miltiorrhiza root, on myocardial ischemia-reperfusion injury in rat due to inhibition of some inflammatory events that occur by NF-kB-activation during ischemia and reperfusion. Myocardial ischemia and reperfusion injury was induced by occluding the left anterior descending coronary artery for 30 min followed by either 2 h (biochemical analysis) or 24 h (myocardial function and infarct size measurement) reperfusion. CTS injected (i.v.) 10 min before ischemia and reperfusion insult. CTS significantly reduced the infarct size and improved ischemia and reperfusion-induced myocardial contractile dysfunction. Furthermore, CTS inhibited NF-kB translocation, expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), neutrophil infiltration and MPO activity in ischemic myocardial tissues. CTS also significantly reduced plasma levels of TNF-α, IL-1β due to ischemia and reperfusion. Interestingly, H<SUB>2</SUB>O<SUB>2</SUB>-stimulated NF-kB-luciferase activity and TNF-α-induced expression of vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) expressions in human umbilical vein endothelial cells (HUVEC) were significantly inhibited by CTS. Taken together, it is concluded that CTS may attenuate ischemia and reperfusion-induced microcirculatory disturbances by inhibition of proinflammatory cytokine production, reduction of neutrophil infiltration and possibly inhibition of adhesion molecules through inhibition of NF-kB-activation during ischemia and reperfusion.
볏짚의 사료가치 증진을 위한 적정 알카린 H2O2 처리 수준에 관한 연구
문양수(Y . S . Moon),하종규(J . K . Ha),고종열(J . Y . Ko),최연호(Y . H . Choy),조경훈(G . H . Cho),최윤재(Y . J . Choi),한인규(I . K . Han) 한국축산학회 1990 한국축산학회지 Vol.32 No.10
This study was carried out to determine the adequate levels of alkaline hydrogen peroxide treatment for the improvement of nutritive value of rice straw. In vitro digestibility and chemical analysis after several treatments were measured. Treatment variables were soaking time(12, 24, 48 and 96 hrs), temperature(5, 25, 50 and 75℃) of alkaline H₂0₂ solution, H₂0₂ concentration (0.5, 1, 2, 3 and 4%) and substrate /solution ratio (1, 2, 3, 4 and 5g/ml). The results obtained were summarized as follows. 1. The DM digestibilities or rice straw treated with H₂O₂ for 24 and 48 hours were higher than those of the others(p$lt;0.05). There was no differ ences when the rice straw was treated for 12, 72 or 96 hours. Cell wall contents were not affected by the time of treatment. 2. The DM digestibilities were higher at pH 11.5 or above(p$lt;0.05) and was decreased as pH declined. When rice straw was treated with H₂O₂ at pH 11.5 or higher, NDF, ADF and cellulose contents were increased. However, lignin content and DM recovery percentages were decreased. 3. The DM digestibilities were not influenced by the ratio of straw versus liquid. The contents of NDF and cellulose were not affected up to the ratio of 4g rice straw/50m1, but were decreased at 5g rice straw/50m1. Lignin content and DM recovery percentages, however, were increased at 5g rice straw /50m1. 4. The DM digestibilities were not different among treatments at 5, 25 and 50℃. However, the rice straw treated at 75℃ showed lowest digestibility of all treatment (p$lt;0.05). The content of NDF, ADF and cellulose was increased by increasing temperature. However, lignin content and DM recovery percentages were decreased by increasing temperature. 5. The AHP treated rice straw had higher content of ADF and cellulose, and lower content of hemicellulose and lignin as the concentration of H₂O₂, increased. But hemicellulose, lignin and DM recovery percentages were decreased. The NDF content was not different among five different concentrations of H₂0₂ solution. In conculsion, based upon the results of present experiments the most desirable method is to soak rice straw in 1% alkaline H₂0₂ solution at pH 11.5, at room temperatrve (25℃), for 24∼48 hours and at the ratio of 4g rice straw /50m1 solmtion.
Hybrid architecture of rhodium oxide nanofibers and ruthenium oxide nanowires for electrocatalysts
Kim, Y.L.,Ha, Y.,Lee, N.S.,Kim, J.G.,Baik, J.M.,Lee, C.,Yoon, K.,Lee, Y.,Kim, M.H. Elsevier Sequoia 2016 JOURNAL OF ALLOYS AND COMPOUNDS Vol.663 No.-
We report the synthesis and electrochemical performances of the hybrid architecture of rhodium oxide (Rh<SUB>2</SUB>O<SUB>3</SUB>) nanofibers (NF) and highly single crystalline RuO<SUB>2</SUB> nanowires (NW) by combining the electrospinning process and a simple recrystallization process. The amorphous Ru(OH)<SUB>3</SUB>.xH<SUB>2</SUB>O precursors at relatively low temperature were efficiently transformed into highly single crystalline RuO<SUB>2</SUB> nanowires with the tetragonal rutile structure on electrospun Rh<SUB>2</SUB>O<SUB>3</SUB> nanofibers. Pure Rh<SUB>2</SUB>O<SUB>3</SUB> NF and hybrid RuO<SUB>2</SUB> NW-Rh<SUB>2</SUB>O<SUB>3</SUB> NF exhibited different electroactivities toward H<SUB>2</SUB>O<SUB>2</SUB> electrochemical reaction: Rh<SUB>2</SUB>O<SUB>3</SUB> NF facilitates the H<SUB>2</SUB>O<SUB>2</SUB> oxidation vs. hybrid RuO<SUB>2</SUB> NW-Rh<SUB>2</SUB>O<SUB>3</SUB> NF promotes H<SUB>2</SUB>O<SUB>2</SUB> reduction more favorably. The H<SUB>2</SUB>O<SUB>2</SUB> reduction free from O<SUB>2</SUB> reduction interference at RuO<SUB>2</SUB> NW-Rh<SUB>2</SUB>O<SUB>3</SUB> NF is advantageous and finds the feasibility for selective H<SUB>2</SUB>O<SUB>2</SUB> detection in various samples. Furthermore, RuO<SUB>2</SUB> NW-Rh<SUB>2</SUB>O<SUB>3</SUB> NF generated a greatly higher current induced by H<SUB>2</SUB>O<SUB>2</SUB> reduction (i.e., enhanced sensitivity to H<SUB>2</SUB>O<SUB>2</SUB>) than bare Rh<SUB>2</SUB>O<SUB>3</SUB> NF.
Simulations of background sources in AMoRE-I experiment
Luqman, A.,Ha, D.H.,Lee, J.J.,Jeon, E.J.,Jo, H.S.,Kim, H.J.,Kim, Y.D.,Kim, Y.H.,Kobychev, V.V.,Lee, H.S.,Park, H.K.,Siyeon, K.,So, J.H.,Tretyak, V.I.,Yoon, Y.S. Elsevier 2017 Nuclear instruments & methods in physics research. Vol.855 No.-
<P><B>Abstract</B></P> <P>The first phase of the Advanced Mo-based Rare process Experiment (AMoRE-I), an experimental search for neutrinoless double beta decay ( 0 ν β β ) of <SUP>100</SUP>Mo in calcium molybdate ( <SUP> 40 </SUP> Ca<SUP>100</SUP>MoO<SUB>4</SUB>) crystal using cryogenic detection techniques, is in preparation at the YangYang underground laboratory (Y2L) in Korea. A GEANT4 based Monte Carlo simulation was performed for the first-phase AMoRE detector and shield configuration. Background sources such as <SUP>238</SUP>U, <SUP>232</SUP>Th, <SUP>235</SUP>U, and <SUP>210</SUP>Pb from inside the crystals, surrounding materials, outer shielding walls of the Y2L cavity were simulated. The estimated background rate in the region of interest was estimated to be < 1.5 × <SUP> 10 − 3 </SUP> counts/keV/kg/yr (ckky). The effects of random coincidences between backgrounds and two-neutrino double beta decays of <SUP>100</SUP>Mo as a potential background source were estimated to be < 2.3 × <SUP> 10 − 4 </SUP> ckky.</P>
Jeong, J.H.,Oh, Y.J.,Lho, Y.,Park, S.Y.,Liu, K.H.,Ha, E.,Seo, Y.H. S.E.C.T. [etc.] ; Elsevier Science Ltd 2016 European journal of medicinal chemistry Vol.124 No.-
The molecular chaperone Hsp90 plays an important role in cancer cell survival and proliferation by regulating the maturation and stabilization of numerous oncogenic proteins. Due to its potential to simultaneously disable multiple signaling pathways, Hsp90 has emerged as an attractive therapeutic target for cancer treatment. In this study, the design, synthesis, and biological evaluation of a series of Hsp90 inhibitors are described. Among the synthetic compounds, 6,7-dihydrothieno [3,2-c]pyridin-5(4H)-yl amide 19 exhibits a remarkable binding affinity to the N-terminus of Hsp90 in a fluorescence polarization (FP) binding assay (IC<SUB>50</SUB> = 50.3 nM). Furthermore, it effectively inhibits the proliferation of H1975 non-small cell lung cancer (NSCLC) and Skbr3 breast cancer cell lines with GI<SUB>50</SUB> values of 0.31 μM and 0.11 μM, respectively. Compound 19 induces the degradation of the Hsp90 client proteins including EGFR, Her2, Met, c-Raf, and Akt, and consequently promotes apoptotic cancer cell death. Compound 19 also inhibits the growth of H1975 xenografts in NOD-scid IL2R gamma<SUP>null</SUP> mice without any apparent body-weight loss. The immunohistologic evaluation indicates that compound 19 decreases the expression of Akt in xenograft tumor tissue via an inhibition of the Hsp90 chaperon function. Additionally, the cytochrome P450 assay indicates that compound 19 has no effect on the activities of five major P450 isoforms (IC<SUB>50</SUB> > 50 μM for 1A2, 2C9, 2C19, 2D6, and 3A), suggesting that clinical interactions between compound 19 and the substrate drugs of the five major P450 isoforms are not expected. Overall, compound 19 represents a new class of Hsp90 inhibitor with its 6,7-dihydrothieno[3,2-c]pyridin-5(4H)-yl-amide structure, and it has the therapeutic potential to overcome drug resistance in cancer chemotherapy.