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고온·알칼리성 Bacillus sp.의 Catabolite Repression 저항성 변이주로부터 Cellulase 및 Xylanase의 생산
최수철,정영철,강갑석,심기환,성낙계 慶尙大學校 1990 論文集 Vol.29 No.2
고온·알칼리성 cellulase 및 hemicellulase 생성 미생물을 분리할 목적으로 pH 10.3의 PY-CMC배지에서 55℃로 3회 연속 직접배양하여 효소 생성능이 우수한 20여균주를 분리하였고, 그 중에서 2개균주를 최종 선정하여 균학적 성상을 조사한 결과 고온·알칼리성 Bacillus속과 거의 일치하였다. 선정, 동정된 고온·알칼리성 Bacillus sp. F204와 F1020은 α-1, 4-glucose 등에서는 효소 생합성이 왕성하였으나, glucose와 같은 단당류에서는 효소가 전혀 유도되지 않았는데, 이 두 균주는 carbon catabolote repression을 받고 있었다. 2-deoxy glucose는 야생주에서는 강력한 repressor로 작용하였고, 야생주의 catabolite repression을 해제 시키는데 antimetabolite로 작용한다는 것이 밝혀졌다. NTG(500㎍/㎖, 50℃, 20∼60분)와 UV(2분) 처리후 2-DG(20mM)에 직접배양하여 강력한 catabolite repression resistant strain인 M-211과 M-367을 분리하였다. M-211과 M-367은 2-DG를 energy원으로 이용하였으며, glucose에 대하여 CMCase는 1.5%, avicelase 및 xylanase는 약 1.0%까지 저항성을 보였다. 또한 두개의 변이주의 효소 활성은 CMCase 2배, avicelase 1.5배 및 xylanase3배의 증가를 보였으며, 각 효소생합성을 위한 gene expression은 cAMP-independent transcription을 하는 것으로 나타났다. Cellulase and xylanase synthesis of thermophilic alkalophilic Bacillus sp. F204 and F1020 isolated in our lab, were repressed by glucose and induced by compounds structurally related to α-1,4 linkage (avicel cellulose and xylan et. al.). Catabolite repression-resistant mutants were obtained through treatment of NTG(500㎍./㎖), UV(2 min) and enrichment culture in 2-deoxy glucose (20 mM) containing PY-glucose on the wild type strain. T재 catabolite repression resistant mutants were not repressed by glucose and produced cellulase complex and xylansase when in grown in the presence of 1.0% glucose and 30mM 2-DC, which were repressors in wild type. Enzyme production was greater with the mutants (CMCase of 2 times, avicelase of 1.5 times, xylanase of 3 times) than with the wild type strains under various test conditions. T재 mutants M-211 and M-367 were potential catabolite repression resistant mutants and their gene expression was cAMP independent transcription.
최종운, 유태환, 김수균, 안성옥 배재대학교 공학연구소 2014 공학논문집 Vol.16 No.1
본 논문은 Unity3D 게임엔진을 이용하여 쉽게 접할 수 있는 아케이드 형식의 미니게임을 제작하는데 목적이 있다. 우리는 미니 게임을 통하여 창의력, 반사 신경을 향상시키도록 하였고, 또한 일상생활에서 볼 수 있는 애완동물이라는 소재를 사용하여, 게임에 접근하기 어려운 사람들에게 친근감을 주게 하는 것을 특징으로 한다. 또한 랭킹 시스템을 도입하여 유저들에게 경쟁을 유도하게 하여 유저들의 게임 참가율을 증대하도록 하였다. 본 논문에서는 Unity3D 엔진을 이용하여, 게임의 개발시간을 단축하여 개발 비용을 줄일 수 있으면서도 게임의 질은 떨어지지 않도록 하였다
한우, 칡소 및 제주 흑우 Calpain-Calpastatin 유전자 다양성
이승환,김승창,조수현,최봉환,Aditi Sharma,임다정,당창권,장선식,김재환,고문석,양보석,강희설 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2
The aim of study was to investigate genetic diversity for the calpain/calpastatin gene in three Hanwoo breeds [(Brown (n=62), Brindle (n=81) and Jeju Black (n=30)]. Random samples from three breeds of Hanwoo were selected and genotyped for the 7 SNPs of calpain/calpastatin using TaqMan method. Allele frequencies were investigated for CAPN1/CAST gene. Allele frequency of CAST2 SNP was 0.75, 0.59 and 0.22 for Brown, Brindle and Jeju black, respectively. The CAST3 revealed allele frequency of 0.59 and 0.57 in Brown and Jeju Black, while it showed very low allele frequency (0.07) in Brindle. In particular, favorable allele (G allele) for the CAPN1-2 SNP which was shown a strong association with tenderness in Taurine and Indicine cattle revealed 16% and 17% higher allele frequency in Brown Hanwoo (0.82) comparing Brindle (0.66) and Jeju Black Hanwoo (0.65). AMOVA demonstrated that among population variance occupied only 10% of total variance and among individual variance was 0%, while within individual variance was 90% of total variance. This result showed that population effect contributed very small portion of genetic to these three Hanwoo breeds, while within individual variance contributed large portion of genetic diversity within these Hanwoo breeds. In conclusion, three Hanwoo breeds (Brown, Brindle and Jeju black) showed a genetically homogeneous based on the 7 SNPs of CAPN1/CAST gene and it came from same ancestor to form modern Hanwoo breed.
손등 정맥 패턴의 회전 왜곡을 보상한 개인 식별 알고리즘의 연구
최환수,안장용 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-
This paper proposes an enhanced algorithm for person identification system utilizing hand vein pattern. The conventional algorithm does not cope with distortion caused by image rotation caused by misplaced hands on the imaging device. A straightforward approach to consider the rotaional compensation required too much computational load, thus, we devised an approach to expect the rotation direction along with image translation, reducing the compuational requirement dramatically. In this paper, we present the detail of the algorithm with experimental results with the new algorithm.
부적절한 예방화학요법 후 지연 발현된 Plasmodium vivax 말라리아 1 예
최수철,장상필,김상화,서환조,서진태,김구엽 대한내과학회 1998 대한내과학회지 Vol.54 No.3
Malaria is the world's most important parasitic infection. Although it has been erw5cated from temperate zones including Korea, increasing numbers of travellers visit tropical malarious countries and imported malaria becomes important medical problem in the developed countries. In Korea with increasing travellers to malaria-endemic area, the incidence of imported malaria shows rising tendency same as the developed countries. It becomes important to personal general personal protective measures and chemoprophylaxis to trevellers, when employed in appropriate manner, that can be highly effective in preventing malaria. We recently experienced a case of in Plasmodium vivax malaria with delayed manifestations due to inadequate chemoprophylaxis. A 53-year-old woman with history of trevel to East Africa 4 months ago and chief complaint of fever was diagnosed as tertian malaria. She had irregularly taken prophylatic antimalarial during travel and had not taken it after return but should have taken it at least 4 weeks after return.
클러스터링 기법을 이용한 손가락 마디지문 식별 알고리즘
최환수,주일용 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-
This paper proposes a finger crease pattern identification algorithm utilizing a clustering method. The algorithm has been developed for the use of biometric person identification system. Since the finger crease pattern may be well-imaged utilizing low cost imaging devices such as low-end CCD camera with LED lighting, the feasibility of commercialization of the algorithm and the system utilizing the proposed algorithm may be justified if the finger crease pattern is a reasonable choice for a biometric feature. In this paper, we exploit this possibility and show the potential of using the finger crease pattern as a feature for biometric person identification.
최의환,하정완,김수관,정태영,김수흥 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.3
Modified surgical technique for transposition of the inferior alveolar nerve followed by immediate placement of endosseous implants in mandibles with moderate to severe atrophy are presented. Five transpositions of the inferior alveolar nerve together with the installation of 10 implants were performed in four patients. The mean postoperative follow-up time was 17 months, with a range of 8 to 20 months. All implants with functioning pontics remained stable, with no mobility or symptoms of pain and infection during the follow-up period. Neurosensory evaluation was performed using the two-point discrimination test. Two patients had objective neurosensory dysfunction at postoperative, but all the nerve function were reported as normal by the patients 4 months postoperatively.
최환수,이종기 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-
The Naming Service is central to most common object request broker architecture(CORBA) applications. It serves as a directory for CORBA objects-the building blocks of CORBA applications. As the Windows computing environment is disseminated, the performance features of the existing CORBA is becoming of great interest. Thus, in this paper, we intend to examine some performance features of three commerically-available Naming Service implementations of CORBA in strictly Windows (NT and 98) environment.