원발성 위암조직에서 fragile histidine triad 유전자의 이상 발현에 대한 연구

조이현,이상한,우기민,송호연,조만희 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background : The fragile histidine triad(FHIT) gene, located at human chromosome 3p14.2, has been shown to produce aberrant transcripts in a number of human cancers. Also, FHIT protein is supposed to have tumor suppressor function. Methods : To determine the role of the FHIT gene in gastric carcinoma, FHIT mRNA transcripts were examined in 16 primary adenocarcinomas by reverse transcription-polymerase chain reaction(RT-PCR). Expression of FHIT protein was measured by immunohistochemistry. Results : In this study, we found normal and abnormal RT-PCR products in 7 cases, and no transcript in one case. Positive immunostaining was observed in normal gastic gland, whereas FHIT immunoreactivity was significantly decreased or absent in most cases tested. Gastric cancer is thought to be develop from carcinogenic exposure, possibly accompanying with the high frequency of the FHIT gene abnormalities. Conclusion : The above results suggest that the FHIT gene can be a preferential target of environmental carcinogens, and the FHIT inactivation may play a role in the gastric carcinogenicity.

원심펌프 성능에 관한 실험적 연구 (II)

조만호,현동수 大田産業大學校 2001 한밭대학교 논문집 Vol.18 No.-

This work is to investigate effect of operating characteristics of pump according to the rotational speed of pump and the change of flow rate when centrifugal pump operates with the condition that regulated flow, head, revolution number, and specific speed are respectively 0.7 ㎥/min, 8 m, 1750 rpm, 180 (m, ㎥/min, rpm). The pump used in the experiment doesn't have a guide vane and directly connects with outside rim so that the rotational speed of volute pump in spiral or volute casing increase by 100 rpm from 1450 until it reaches 1750. The results of the relationship of Q-H, Q-L, Q-η and the dimensionless performance features such as head coefficient, power coefficient and efficiency are as follows; (1) According to the increase of evolution number, the head and power increase up to the square or cube of the evolution number. At the same evolution number, the power is increased according to the flow. In time of standard operation, the head has a stable feature in the early stage of flow increase from the segment point. (2) The pump's highest efficiency shows 52% with flow rate 0.17㏄/min and head 4.7 m in time of 1450 rpm and 50% with flow 0.18㎥/min and head 6.7 m in the pump's highest rotational speed of 1750 rpm. (3) In each rotational speed, curve of efficiency, head and power is different. Meanwhile, the dimensionless performance valuables show a line irrespective of rotational speed. As flow coefficient increases, power coefficient increases and head coefficient decreases. When the flow coefficient is 0.08, it reaches the maximum efficiency 52%.

면역학적 특성에 따른 yeast cytochrome c의 구조에 관한 연구

조경환,우기민,조만희,이상한,박현경,김창세 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

The antiserum against yeast cytochrome c and horse heart cytochrome c was reacted immunologically with those from various species. The results were as followings; 1. The antiserum against horse heart cytochrome c was reacted immunologically with heart cytochrome c of horse, cow, rabbit, mouse, chicken, pigeon, frog, tuna, and guanaco, and were not with yeast and neurospora cytochrome c. 2. However, antiserum against yeast cytochrome c was only reacted immunologically with yeast cytochrome c, and was not mammals and vertebrates horse, cow, rabbit, mouse, chicken, pigeon, frog, tuna, guanaco, even neurospora 3. CNBr-digested peptides of horse heart cytochrome c were obtained 5 peaks, Ⅰ(1-80), Ⅱ(1-65), Ⅲ(66-104), Ⅳ(81-104), Ⅴ(66-80), and other peptide (1-50). Among them peptides Ⅰ and Ⅱ were weakly reacted immunologically with antiserum against horse heart cytochrome c. CNBr-digested peptides of horse heart cytochrome c were not reacted with antiserum against yeast cytochrome c. 4. From the above, the immunologically positive cross reaction was thought to be related with Ω loop D.

쑥을 첨가한 두부의 제조 및 저장 중 품질변화

조덕제,정숙현,조만기 東西大學校 1996 동서논문집 Vol.2 No.1

쑥의 효과적인 이용방법을 위해서 쑥의 최적건조 조건을 설정하고, 대두와 숙의 기능적 특성을 잘 살린 쑥을 첨가한 두부의 최적 가공조건과 제품의 저장 중 품질변화에 대해서 실험한 결과를 요약하면 다음과 같다. 1. 건조과정 중 쑥은 조단백질, 조지방 및 조회분은 감소하였으며, 반면 탄수화물은 증가하였다. 2. 1% NaHCO3에 데치기 하여 동결건조한 제품(FDB)이 쑥의 색도유지가 가장 잘 되었으며, Chlorophyll 잔존률도 가장 많았다. 3. 쑥을 첨가한 두부의 제조에서 응고제 GDL, 응고농도 0.1%, 응고온도 70-80℃, 쑥분말 첨가량 0.2%가 최적가공 조건이었다. 4. 쑥을 첨가한 두부의 저장에서 색도변화 및 TBA가의 변화로 미루어 보아 30℃에서는 12시간, 5℃에서는 4일 정도까지 저장 가능한 것으로 판단되었다. An investigation was carried out to study the optimum drying condition of mugwort, the optimum processing conditions of soybean curd added mugwort, and the quality change during storge of soybean curd added mugwort. Crude protein and crude lipid contents of mugwort were decreased during drying process, the contents of those in dried mugwort were 18.0-21.2%, 1.3%-4.0%, respectively. The freeze drying after blanching in 1% NaHCO3 solution(FDB) was the best in color index of dried mugwort, also the highest in retention ratio of total chlorophyll content. The yield of soybean curd with coagulants were showed that MgCl2, CaCl2, CaSO4 and GDL were 3.4g, 3.0g, 3.5g and 4.2g per 30g soy milk, respectively. GDL showed the highest yield among coagulants, and amount of GDL was suited at 0.1%. The optimum coagulation temperature was 70-80℃, addition amount of powered mugwort was 0.2%. The change of color index and TBA value were increased during storage. The quality of soybean curd added mugwort was maintained up to 12 hrs or 4 days at 30℃ or 5℃ storage, respectively.

遠心펌프 性能에 關한 實驗的 硏究Ⅰ

조만호,현동수 大田産業大學校 2000 한밭대학교 논문집 Vol.17 No.1

This study is to investigate effect of operating characteristics of pump according to the rotational speed of pump and the change of flow rate when centrifugal pump operates with the condition that regulated flow rate, head, revolution number, and specific speed are respectively 0.7m3/min, 8 m, 1750 rpm. 180(m, m3/min. rpm). The pump used in the experiment doesn't have a guide vane and directly connects with outside rim so that the rotational speed of volute pump in spiral or volute casing increase by 100 rpm from 1350 until it reaches 1750 rpm with five phases. The study results of the relationship of H-Q features, L-Q features, η-Q and dimensionless performance characteristics. (1) According to the increase of rotational speed, the head and power increase up to the square or cube of the rotational speed. At the same rotational speed, the power is increased according to the flow rate. In time of standard operation, the head has a increase characteristics in the early stage of flow rate. (2) The maximum efficiency shows 53% at 0.170.7m3/min and head 4.7m of 1450 rpm and 51% with 0.180.7m3/min and head 6.7m in the pump's highest rotational speed of 1750 rpm. (3) In the curve of performance characteristics of flow rate vs. head, the constant efficiency curve changes into oval shape without becoming the quadratic equation curve that passes through the origin coordinates according to pump's similarity law.

부산의 한 대학병원에서 분리된 Acinetobacter baumannii의 PER-1 Extended-Spectrum β-Lactamase 생성 현황

김정만,강현경,정석훈,배일권,권수봉,조병규,김둘만,김현주,김아성 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

Dunaliella salina의 성장에 미치는 탄소원 및 빛의 영향

이지현,이정석,조만기 동서대학교부설연구소 1998 연구소 논문집 Vol.3 No.-

The marine microalga Dunaliella salina was studied to optimize its growth conditions in batch cultrue. Various culture conditions were studied by adding glucose and NaHCO??. Another culture methods like photoautotrophic, photoheterotrophic and heterotrophic conditions were studied. The adding 0.5% glucose in the medium made the growth rate increased more than 2 times, but the adding NaHCO?? had no effect on the growth rate of Dunaliella salina. Dunaliella salina did not grow in heterotrophic condition but grew rapidly in photoheterotrophic condition with light and glucose.

알코올 및 인삼액이 효소활성도에 미치는 영향에 관한 연구

신경현,조만희,김창세 순천향대학교 1994 논문집 Vol.17 No.2

This study was investigated for the change of r-GTP, CPK activity and CPK-isoenzyme by alcohol and Ginseng administration, The normal white S.D rats were classified with four groups; ethanol treated was group A, ethanol pretreated group combined pretreated with Ginseng extract was group B, Ginseng extract treated was group C, and Ginseng extract treated group combined pretreated with ethanol was group D. The white rats of four groups were treated with 20%, 30%, 40% ethanol and/or 1% Ginseng extract. Then enzyme activities such as r-GTP, CPK and CPK-isoenzyme had been measared in serum, hepatic, and renal tissues. The sample was collected by 5 day intervalus for 25 days. The following results were obtained. 1. The changes of serum r-GTP activities were significantly increased in ethanol-treated group, decreased in Ginseng-treated group. 2. The changes of hepatic r-GTP activites were significantly increased in ethanol-treated group, decreased in Ginseng-treated group. 3. The changes of renal r-GTP activites were significantly increased in ethanol-treated group, decreased in ginseng-treated group. 4. The changes of serum CPK activites were significantly increased in ethanol-treated group, decreased in ginseng-treated group. 5. The changes of hepatic and renal tissue CPK activites were not significant. 6. The changes of serum and hepatic tissue CPK-isoengyme were significantly increased in ethanol-treated group, decreased in ginseng-treated group combined pretreated with ethanol. 7. The changes of CPK-isoengyme, CPK 1 and CPK 2 were not significant.

아므르불가사리 (Asterias amurensis)의 배우자를 이용한 남해해역 연안해수의 생물학적 수질평가

유춘만,박지인,조기안,주현수,박종천,나명석 한국환경과학회 1999 한국환경과학회지 Vol.8 No.1

This study was carried out to evaluate off-shore water quality and to draw marine pollution map to Southern sea in Korea by bioassay using gametes and early development system of Asterias amurensis. From the bioassay, it was determined that the water qualites of Southern sea maintained the grade Ⅱ, because development of A. amurensis were moderately inhibited. However, Sunchon, Kwangyang, and Jinhae Bay belonged to grade Ⅲ, because development of A. amurensis were strongly inhibited. This could be due to the inflows of several river systems and poor water circulation. The water quality at Yosu island was the grade I, because A. amurensis were not inhibited in its early development.

Metro optical Ethernet 소프트웨어의 연구

방만원,박종현,이명은,최명선,김명조 木浦大學校 應用科學硏究院 2001 應用科學硏究誌 Vol.1 No.-

본 논문에서는 메트로 옵티컬 이더넷의 소프트웨어 구조에 관한 연구를 위하여 10기가 비트 이더넷 스위치에 적용될 QoS 지원 소프트웨어의 적용성을 자원할당 방식 시그널링 프로토콜인 RSVP와 트래픽 특성에 따라 CoS를 구분하는 DiffServ가 10 기가비트이더넷 스위치에 활용 가능한지를 네트웍크 프로세스 처리 능력과 확장성 측면에서 분석하였다. 결론적으로 RSVP, Diffserv와 802.1p는 구현 및 상혼 연동이 가능하다. 그러나 이것은 기업 내부망으로 한정된다는 단점을 가지고 있다. 일반적으로 중소규모의 특정 기업의 내부망에 위의 기술들을 구현하는 것은 어려움이 없다. 그러나 공중망에서의 적용은 아직까지는 어려움이 많이 존재하며 서비스 제공자간의 연동은 거의 불가능하다. 따라서 이러한 기술들은 End-to-End QoS를 보장하는 것에 목적을 두고 있다.