Snail1 is stabilized by O-GlcNAc modification in hyperglycaemic condition

Park SY,Kim HS,Kim NH,Ji S,Cha SY,Kang JG,Seo HK,Ota I,Shimada K,Konishi N,Nam HW,Hong SW,Yang WH,Roth J,Yook JI,Cho JW 한국당과학회 2011 한국당과학회 학술대회 Vol.2011 No.1

Protein O-phosphorylation often occurs reciprocally with O-GlcNAc modification and represents a regulatory principle for proteins. O-phosphorylation of serine by glycogen synthase kinase-3β on Snail1, a transcriptional repressor of E-cadherin and a key regulator of the epithelial-mesenchymal transition (EMT) programme, results in its proteasomal degradation. We show that by suppressing O-phosphorylation-mediated degradation, O-GlcNAc at serine112 stabilizes Snail1 and thus increases its repressor function, which in turn attenuates E-cadherin mRNA expression. Hyperglycaemic condition enhances O-GlcNAc modification and initiates EMT by transcriptional suppression of E-cadherin through Snail1. Thus, dynamic reciprocal O-phosphorylation and O-GlcNAc modification of Snail1 constitute a molecular link between cellular glucose metabolism and the control of EMT.

단속적 원형봉합 (Interrupted Circular Suture) 전치 유착태반 제왕절개 수술시 지혈방법

차경섭,계정웅,조주연,차광열,김수연 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.12

전치태반으로 제왕절개 수술시 때때로 태반박리부위부터 짧은 시간에 많이 나오는 출혈은 수술시야를 흐리게 하고 급격히 환자의 혈압을 떨어뜨리게 된다. 전치태반으로 제왕절개 수술을 받은 120예의 환자중에서 출혈부위의 단순봉합으로 지혈이 되지 않거나 태반박리 부위로부터 짧은 시간에 많이 나오는 출혈을 보이는 환자 20예에서 단속적 원형봉합수술을 시행하였으며 결과는 다음과 같다. 1. 유착 전치태반이 있는 산모 20예의 제왕절개 수술에서 19예는 단속적 원형 봉합수술로 지혈에 성공하여 자궁적출 수술을 피할 수 있었으며 1예는 자궁적출 수술을 하였다. 2. 이 새로운 수술방법은 전치태반으로 제왕절개 수술시 자궁적출을 피하면서 지혈을 시도할 때 출혈량이나 수술시간을 감소시킬 수 있을 것이다. 3. 전치태반으로 인한 제왕절개 수술시 자궁적출을 해결책으로 사용하는 빈도를 크게 감소시킬 수 있을 것으로 사료된다. Rapid accumulation of blood from the placental separation site during cesarean delivery for placenta previa sometimes obscures the surgical field and quickly leads to deterioration of the patient`s vital signs. We used the following technique in 20 cases of intractalbe bleeding among 120 cesareans for placenta previa. Following failure to control the bleeding by suture at the placental separation site via the lower segment cesarean incision, the vessels were ligated using interrupted 2-3 cm sutures at 1cm intervals in a circle around the bleeding area on the serosal surface of the uterus. The sutures were placed as deeply as possible in order to reach the endometrium. This led to marked decrease in bleeding and allowed the small vessels to be easily identified and ligated. The results were as follows. 1. The amount of blood transfused and the operation time were gradually reduced as we became adapt in the use of this ptocedure. 2. Our experience suggests that this technique could reduce the use of hysterectomy in cesarean section for placenta previa.

Effect of Weight Loss on Cerebrospinal Fluid and Plasma Concentrations of NPY, Galanin, CRH and Leptin in Obesc Women

(SY Nam),(KR Kim),(BS Cha),(YD Song),(SK Lim),(HC Lee),(KB Huh) 대한비만학회 2000 대한비만학회 학술대회 Vol.9 No.1

Comparison of Postmortem Proteolysis between Breast and Leg Muscles in Chiayi Native Chickens

Cha, Shih-Ting,Tseng, Tsai-Fuh,Ho, Sy-Shyan,Chou, Rong-Ghi R. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.5

Postmortem Proteolysis of breast (BM) and leg (LM) muscles in Chiayi native chickens at $5^{\circ}C$ were compared. Myofibrils were purified from BM and LM samples that were randomly taken from carcasses after 0, 1, 3, 7 and 14 days of storage at $5^{\circ}C$. Fragmentation of myofibrils were determined, and degradation of myofibrillar proteins were analyzed by the SDS-PAGE and western blots. The results showed that myofibril fragmentation index (MFI) was significantly (p<0.05) higher in BM than in LM samples. Disappearance of titin and nebulin and appearance of the 30 kDa component were more rapidly as seen on SDS-PAGE in BM than in LM samples. Western blots labeled with a monoclonal antibody to desmin also demonstrated that desmin degraded more quickly in BM samples. Our data suggested that postmortem proteolysis occurred more rapidly in breast muscles in Chiayi native chickens.

Effect of Weight Loss on Cerebrospinal Fluid and Plasma Concentrations of NPY, Galanin, CRH and Leptin in Obese Women

Nam, SY,Kim, KR,Cha, BS,Song, YD,Lim, SK,Lee, HC,Huh, KB 대한비만학회 2000 The Korean journal of obesity Vol.9 No.1

Photoluminescence enhancement of quantum dots on Ag nanoneedles

Ahmed, Syed Rahin,Cha, Hee Ryoung,Park, Jung Youn,Park, Enoch Y,Lee, Dongyun,Lee, Jaebeom Springer 2012 NANOSCALE RESEARCH LETTERS Vol.7 No.1

<P>Noble metal nanostructure allows us to tune optical and electrical properties, which has high utility for real-world application. We studied surface plasmon-induced emission of semiconductor quantum dots (QDs) on engineered metallic nanostructures. Highly passive organic ZnS-capped CdSe QDs were spin-coated on poly-(methyl methacrylate)-covered Ag films, which brought QDs near the metallic surface. We obtained the enhanced electromagnetic field and reduced fluorescence lifetimes from CdSe/ZnS QDs due to the strong coupling of emitter wave function with the Ag plasmon resonance. Observed changes include a six-fold increase in the fluorescence intensity and striking reduction in fluorescence lifetimes of CdSe/ZnS QDs on rough Ag nanoneedle compared to the case of smooth surfaces. The advantages of using those nanocomposites are expected for high-efficiency light-emitting diodes, platform fabrication of biological and environmental monitoring, and high-contrast imaging.</P>

ANCA와 연관된 혈관염 및 사구체 신염에서 환자 생존 및 신장관해에 대한 연구

한상엽,권영주,신진호,조상경,차대룡,조원용,표희정,김형규,윤수영 대한신장학회 1999 Kidney Research and Clinical Practice Vol.18 No.3

ANCA-associated vasculitis and glomerulonephritis encompasses a variety of conditions such as Wegener's granulomatosis(WG), microscopic polyangiitis (MP), Churg-Strauss syndrome(CS) and renal-limited vasculitis[pauciimmune necrotizing cre sctric glomerulonephritis(NCGN)]. To analyze risk factors for poor patient survival and renal remission, we examed sixteen patients diagnosed as ANCA-associated vasculitis and glomerulonephritis according to age, ANCA, extrarenal vasculitis and a presenting serum creatinine level. Diagnosis of sixteen patients(male 10/ female 6, mean age 51.2 years, range 21-74 years) were 5 WG, 4 MP, 1 CS, and 6 NCGN. Ten patients were ANCA positive(c-ANCA 5, p-ANCA 5) and six patients were ANCA negative. Six patients showed extrarenal vasculitis, especially lung involvement. The median value of a presenting serum creatinine was 4.55mg/dl(range 0.3-18mg/dl). According to age, older patients than 60 years(n=7) are at greater risk than younger patients in patient survival(n=9)(P= 0.01). In patient survival and renal remission, patients who present serum creatinine above 4.55mg/ dl(n=8) are at greater risk than below(n=8)(P=0.018, P=0.02). The other factors such as ANCA and extrarenal vasculitis did not influence on patient survival and renal remission. In conclusion, the poor prognostic factors were older age above 60 years of age and a presenting serum creatinine level above 4.55mg/dl. Therefore, patient survival and renal remission appear to have improved with early diagnosis and early recognition of disease activity before serum creatinine rises.

원격 전이를 동반한 과립막세포종 1 예

황태영,차문석,곽재환,김수영,박성용,허기영,제구화,김현호 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.6

본 병원 산부인과에서는 48세 폐경전기의 여성에서 파열된 양측성 과립막세포종으로, 발견 당시 이미 폐전이가 있어 FIGO stage IV로 진단되었고, 조직검사상 세포의 분화도가 나빴으며, 10개의 고배율 시야당 10개 이상의 유사분열을 보이는 과립막세포종 환자를, 적절한 수술적 치료후 BEP regimen으로 보존적 약물치료를 하였으나, 간 및 비장에 새로운 전이가 생겼고, 급성 신부전으로 사망한 일례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Granulosa cell tumor is a rare stromal cell tumor of the ovary, generally considered to be of low-grade malignancy, with an indolent clinical course. Only a small percentage of such tumors metastasize, and they rarely do so to the lung. In this paper, we present a case of bilateral ovarian granulosa cell tumor with lung metastases.

자궁경부의 세포검사에서 비정상소견을 보인 중신관 잔유물 1 예

한상원,차동수,정순희,이상엽,박수희 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.5

자궁경부 세포검사에서 비정상소견을 보인 환자에서 원추생검술을 시행하여 그 검체의 심부에서 중신관 잔유물(mesonephric duct remnant)이 확인되어 자궁적출술을 고려하였던 환자에게 더이상의 수술을 진행하지 않았던 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. The mesonephron is regressed and the uterus is formed by fusion of the Mullerian ducts by the 8th gestational weeks. The mesonephric ducts are occasionally involved in the lower corpus and cervix of the uterus. Mesonephric duct remnants in the uterine cervix is an unusual condition that is often misdiagnosed as adenocarcinoma. Mesonephric hyperplasia does not appear to require therapy, even if it is found at the resection margin of a conization or hyterectomy specimen. It is important to distinguish benign mesonephric lesion from malignancy, to avoid overtreatment.

흡임소파술에 굽은 칼만 (Karman) 씨흡입관 사용

조주연,차선희,성혜리,김수연 대한산부인과학회 1995 Obstetrics & Gynecology Science Vol.38 No.2

주로 자연유산으로 인한 자궁 내용물 제거수술을 할 때 쉽게 굽어지는 플라스틱 흡이관인 칼만씨 흡입관을 굽혀서 사용한 것과 직선인 것을 사용하는 방법의 차이를 알아보기 위해 굽은 칼만씨 흡입관과 곧은 칼만씨 흡입관으로 수술을 하여서 비교한 결과는 다음과 같다. 1. 수술 부작용 발생률을 곧은 칼만씨 흡입관을 사용한 군에서는 17%였고 굽은 칼만씨 흡 입관 사용군에서 10%로서 굽은 칼만씨 흡입관 사용군에서 유의하게 낮았다.(p$lt;0.05). 2. 수술시간은 곧은 칼만씨 흡입관을 사용한 군에서 9.58+-3.46(Mean+-S.D.)분, 굽은 칼만씨 흡입관사용군에서 12.18+-4.86(Mean+-S.D.)분으로서 굽은 칼만씨 흡입관 사용군에서 유의하게 적게 걸렸다(p$lt;0.05). A curved Karman`s cannula that may give the benefit of reducing operating time and complications is introduced for suction crettage. The curved Karman`s cannula was made as follows : First, a 4-7cm portion of the straight Karman`s cannula is exposed to heat. When desired portion becomes soft, it is bent to a desired shape and angle (with 15 and 30), used in accordance with the shape of the uterus. Curved or straight cannulas were used alternatively every other year for 4 years from March 1990 to February 1994. Curved cannulas were used in 380 cases and straight cannuls were used in 423 cases in study period. A comparison study was carried out in 278 cases using the curved cannula and 321 cases using the straight cannula where follow up was possible until the subsequent menstrual period. The results were as follows. 1. The complications rate was 17% using the straignt cannula and 10% using the curved cannula. There was lower complication rate using the curved cannula than the straight one(p$lt;0.05). 2. Operating time was 9.58+-3.46(Mean+-S.D.) minutes using the straight cannula and 12.18+-4.86(Mean+-S.D.) minutes using the curved cannula. It takes shorter operating time using the curved cannula than the straight one(p$lt;0.05).