Redox-sensitive Transcriptional Regulators from Streptomyces coelicolor

Kang, JG,Bae, JB,Hahn, JS,Hahn, MY,Oh, SY,Seok, YJ,Roe, JH 이화여자대학교 세포신호전달연구센터 2000 고사리 세포신호전달 심포지움 Vol. No.2

We are investigating the regulation and the role of several oxidative defense enzyme systems in the growth and differentiation of the antibiotic-producing, Gram-positive bacterium Streptomyces coelicolor A3(2). Genes for catalase A(catA; monofunctional, vegetative catalase}, catalase B(catB; monofunctional, stationary phase specific), catalase C(catC; catalase-peroxidase, late exponential phase-specific), Fe-containing SOD(sodF), and Ni-containing SOD(sodN) have been examined for their regulation and the role in the growth and differentiation of S coelicolor. Transcriptional regulators governing the regulation of these genes in. response to thiol-oxidation(RsrA/SigR), nickel(SodR1), hydrogen peroxide(CatR, OxyR), osmolarity(RsbA/SigB), or others(FurA) were found and partially characterized. SigR(Φ^(R)) is a sigma factor responsible for inducing the thioredoxin system in response to oxidative stress in S coelicolor. A redox-sensitive, Φ^(R)-specific anti-sigma factor, RsrA, was identified. It binds <DR and inhibits Φ^(R)-directed transcription in vitro only under reducing conditions. Exposure to H₂O₂ or to the thiol-specific oxidant diamide caused the dissociation of the Φ^(R)-RsrA complex, thereby allowing Φ^(R)-dependent transcription. This correlated with intramolecular disulfide bond formation in RsrA. Thioredoxin was able to reduce oxidized RsrA, suggesting that Φ^(R), RsrA and the thioredoxin system comprise a novel feedback homeostasis loop that senses and responds to changes in the intracellular thiol-disulfide redox balance. Another redox-sensitive regulator CatR was identified as a repressor for catA gene expression encoding catalase A. The catR gene was isolated as a genetic locus whose mutation caused overproduction of catalase A. The catR gene encodes a 138 amino acids protein(15319 Da) showing homology to Fur proteins of other bacteria. Derepression of transcription from catRp1, catRp2, and catA promoters was observed within 10 min of H₂O₂ treatment, suggesting H₂O₂-mediated modification of CatR activity. Detailed mechanisms for the redox-dependent modulation of these transcriptional regulators are under investigation.

Human Papilloma Virus Type 16 양성 유무에 따른 자궁경부암의 임상양상 및 예후인자에 관한 연구

강순범,이효표,박만철,이경희,장윤석,송계용,박상철,박재갑,안기범 대한산부인과학회 1990 Obstetrics & Gynecology Science Vol.33 No.7

서울대학교병원 산부인과를 내원하여 조직 생검상 자궁경부암으로 진단받은 51예와, 그외의 여성 생식기 암 10예 및 이상소견이 없었던 12예에서 자궁경부조직을 채취하여 HPV 16의 검출을 위한 southern blot 핵산교잡법과 단백질 분해효소의 측정을 통해 다음과 같은 결론을 얻었다. 1. HPV16 DNA는 자궁경부암에서 35.3%(18예)의 양성율을 보였으나 기타의 여성생식기암과 정상자궁경부 조직 소견을 보인 군에서는 전혀 발견되지 않았다. 2. HPV 16 DNA 양성율은 종양의 크기, 세포의 형태, 자궁경부조직 및 자궁방결합 조직의 침윤 정도 및 임파혈관의 침윤과는 유의한 상관관계가 없었으나, 골반내 임파절 전이가 된 군에서 유의하게 증가하였다(p$lt;0.05). 3. 자궁경부암이 있었던 경우에서는 HPV 16 DNA양성군에서 serine protease와 metalloprotease의 활성도가 유의하게 증가하였다(p$lt;0.001). 이상의 결과에서 HPV 16 DNA 양성군에서는 조직침윤성이 높은 것으로 추측이 되는 반면, 자궁경부암의 유발인자로서의 HPV 16의 역할에 대하여는 추후 장기적이고 더 많은 역학적, 분자생물학적 연구가 필요하리라고 사료된다. It is suspected that HPV infections, especially type 16 and 18, influence the biologic behavior of the cervical cancer. This study included 51 cases of biopsy-proven cervical cancer, 10 cases of other genital cancer, and twelve cases of negative biopsy. We have analysed for the presence of HPV deoxyribonucleic acid(DNA)by Southern blot analysis and measured the proteolytic activities of the cervical cancer tissues. Human papilloma virus DNAs were found in 18 of 51(35.3%)women with cervical cancer, but none of women with other genital cancer and negative biopsy. Human papilloma virus 16 positive rate was significantly elevated in group of positive pelvic lymph node metastasis(p$lt;0.05). In cervical tissues of HPV 16 DNA positive cervical cancer group, the activities of serine protease and metalloprotease were increased with statistical significance(p$lt;0.001). The results of this study suggest that the ability of tissue invasion may be high in HPV DNA positives group. But further epidemiological & biological study will be needed to clarify the role of HPV 16 in cervical cancer.

진통 기록지상으로 본 자궁수축

이정기(JG Rhee),박경희(KH Park),강재화(JH Kang),김기진(KJ Kim) 대한산부인과학회 1968 Obstetrics & Gynecology Science Vol.11 No.11

A proper understanding of the characteristics of normal uterine contractility is necessary in order to appreciate the irregularities which exist in abnormal labors and to permit rational treatment. The auther was interested in trying to find out what the cause of this phenomenon might be, and if that were not possible, at least to learn more about the character of the uterine activity which takes place during pregnancy and labor. Uterine contractility has been studied in the past by different methods, each one of which brings into view some special aspect of the uterine function and contributes to a partial underrstanding of problems. An external method for recording simultaneously the activity by tocographic study of the uterus has been developed in the department of obstetrics, Catholic Medical College. The results obtained were summarized as follows: 1. AM & D type tocographic records were made of the uterine contraction patterns of 10 patients while they were experiencing normal pregnancy and labor. 2. As the term approaches, not only greater uterine contraction wave but also smaller one could be traced that were arisen by true and false labor pain. 3. Uterine contraction was observed with increasing frequency and tonus as pregnancy advanced, and it`s greatest increase taking place about the end of the last stage. 4. Characeteristic contraction pattern of each true labor pain presented while it was in 1st, 2nd and 3rd stage and could be understood the whole pergnancy and labor coures by it`s explanation. 5. The uterine contraction had great individual differences.

이동한 자궁내기구에 발생한 방광결석

남재근,강정수,양희탁,최낙규 大韓泌尿器科學會 2003 Korean Journal of Urology Vol.44 No.2

A phenolic compound, 5-caffeoylquinic acid (chlorogenic acid), is a new type and strong matrix metalloproteinase-9 inhibitor

Jin UH,Lee JY,Kang SK,Kim JK,Park WH,Kim JG,Moon SK,Kim CH 한의병리학회 2006 대한동의병리학회 학술대회논문집 Vol.2006 No.9

보조감각운동영역 간질의 수술적 치료 결과

정택근,이정교,강중구,이상암,고태성,강신광,권병덕 대한신경외과학회 2002 Journal of Korean neurosurgical society Vol.31 No.5

The glycemic efficacies of insulin analogue regimens according to baseline glycemic status in Korean patients with type 2 diabetes: sub-analysis from the A₁chieve® study

Hwang, Y-C,Kang, JG,Ahn, KJ,Cha, BS,Ihm, S-H,Lee, S,Kim, M,Lee, B-W Blackwell Publishing Ltd 2014 INTERNATIONAL JOURNAL OF CLINICAL PRACTICE -HOVE- Vol.68 No.11

<P><B>Aims</B></P><P>In this study, we compared the glucose-lowering effectiveness of insulin analogues and their combination according to baseline glycemic status in patients with type 2 diabetes (T2D) from the A<SUB>1</SUB>chieve® study conducted in Korea.</P><P><B>Methods</B></P><P>This sub-analysis from the A<SUB>1</SUB>chieve® study was a 24-week prospective, multicenter, non-interventional, open-labelled study. Of the 4058 patients, 3074 patients who had their HbA<SUB>1c</SUB> level measured at baseline were included in this sub-analysis. We classified patients into three groups according to baseline HbA<SUB>1c</SUB> levels: group I (HbA<SUB>1c</SUB> < 7.5%), group II (7.5% ≤ HbA<SUB>1c</SUB> < 9.0%) and group III (HbA<SUB>1c</SUB> ≥ 9.0%).</P><P><B>Results</B></P><P>Patients in group I showed no significant HbA<SUB>1c</SUB> reduction with any insulin regimens (detemir, aspart, detemir and aspart or biphasic aspart 30 (Novo Nordisk A/S, DK-2880 Bagsværd, Denmark) after 24 weeks of treatment. In group II, although HbA<SUB>1c</SUB> was decreased for all insulin regimens, there was no difference in mean HbA<SUB>1c</SUB> reduction among the four insulin regimens. In patients with a high baseline HbA<SUB>1c</SUB> level (group III), mean HbA<SUB>1c</SUB> reduction was the greatest in patients on a basal-bolus regimen (detemir and aspart, −3.50%) and lowest in patients on a bolus regimen (aspart, −1.81%; p < 0.001).</P><P><B>Conclusion</B></P><P>For optimal glycaemic control, a basal-bolus regimen may be adequate for Korean patients with poorly controlled T2D (HbA<SUB>1c</SUB> ≥ 9.0%).</P>

자궁경관임신의 1 례

유금준(GJ Yoo),강경주(KJ Kang),김중근(JG Kim),곽태로(TR Gwag) 대한산부인과학회 1987 Obstetrics & Gynecology Science Vol.30 No.6

저자들은 개인병원에서 소파수술후 지속적인 성기출혈을 주소로하여 내원한 47세의 경산부에서 희귀한 자궁경관임신을 경험하여 자궁전적출술을 시행하였고 병리조직학적 검사에서 확진되었기에 문헌적 고찰과 함께 보고하는 바이다 A case of cervial pregnancy which is recently experienced in our department is presented with a brief review of related literatures

기능장애성 자궁출혈의 임상 및 병리학적 고찰

김철,지정희,하부수,정종기,강소희 대한산부인과학회 1993 Obstetrics & Gynecology Science Vol.36 No.6

1988년 1월부터 1992년 11월까지 본 병원 산부인과에서 기능장애성 자궁출혈환자 509례에 대한 임상및 조직학적 검사에서 다음과 같은 결과를 얻었다. 1. 연령분포는 40대가 35.4%, 30대가 36.1%, 50대가 13%였다. 2. 평균 임신횟수는 4.1회였고, 분만횟수는 2.1회였다. 3. 출혈양상은 월경간출혈 223례(43.8%), 월경과다 151례(29.5%)로 많은 비중을 차지하였다. 4. 자궁내막의 조직학적 소견은 증식기내막이 310례(60.9%)로 가장 많았으며, 분비기내막 159례(31.2%), 내막증식증이 28례(5.5%) 등이었다. 5. 표준화한 월경주기가 14일후 자궁내막생검을 시행한 209례 중 배란성 출혈을 의미하는 분비기내막은 82례(39%)로서 비배란성출혈이 많았다. 6. 치료는 자궁내막소파술이 292례(57.4%), 자궁내막생검 및 소파수술후 호르몬요법이 191례(37.8%) 였으며 자궁적출술이 21례(5.1%)였다. Dysfunctional Uterine Bleeding is defined as abnormal bleeding from the uterine endometrium, which is unrelated to anatomic lesion of uterus and excluded uterine bleeding secondary to organic lesion of uterus. Dysfunctional uterine bleeding may occur on any age, from menarche to menopause, especially occurs periods of puberty and menopause easily. This Study is reported on clinical and pathologic aspects in 509 cases of dysfunctional uterine bleeding, and brief review of literature. The results were as follows. 1. Age distribution of dysfunctional uterine bleeding was mainly 4th & 5th decades. 2. Obstetric history of patients with dysfunctional uterine bleeding showed 2~3 times deliveries and 4~5 times pregnancies. 3. Among variable patterns, intermenstrual bleeding (43.8%) was most frequent and menorrhagia (29.5%) was next. 4. Histologic findings of endometrium were proliferative phase, 310 caes (60.9%), secretory phase, 159 cases (31.2%) and hyperplasia, 24 cases (5.5%) in order of frequency. 5. According to endometrial biopsy, the incidence of on anovulatory DUB was 61% (82 cases out of 209 cases) and ovulatory DUB was 39% (82 cases out of 209 cases). 6. Treatment were made by endometrial currettage(57.4%), Hormonal therapy (37.8 %) & Hysterectomy (5.1%).

45,tX,dic(X;X)(q28;q26),der(13;14)(q10;q10)/44,X,der(13;14)(q10;q10) 핵형을 보이는 터너 증후군 1 예

임헌정,기광수,양승옥,박중기,강기철,이장용 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.7

저자들은 사춘기 지연 및 원발성 무월경을 주소로 본원 산부인과에 내원한 16세 여아에서 X 염색체 모자이시즘과 상염색체의 로�맒�전좌를 동반한 45,tX,dic(X; X)(q28;q26),der(13;14)(q10;10)/44,X,der(13;14)(q10;q10) 핵형을 보이는 터너 증후군 1예를 경험하였기에 간단한 문헌 고찰과 함께 보고하는 바이다. Turner syndrome, the most common female gonadal dysgenesis, is characterized by short stature, sexual infantilism, neck webbing, cubitus valgus and low posterior hairline, which is often associated with many variant mosaicisms and aberrations of X chromosome. We experienced a case of 16-year-old phenotypic female examined because of delayed puberty and primary amenorrhea, and showing 45,tX,dic(X;X)(q28;q26),der(13;14)(q10;q10)/44,X,der(13;14)(q10;q10) karyotype. To our knowledge, this karyotype may be a very rare variant of Turner syndrome, and we report this case with brief review of related literatures.