직류전위차법에 의한 피로균열 진전속도 측정시스템의 제작

배성인,차정환,김종섭,최수준 國立 昌原大學校 産業技術硏究所 1995 産技硏論文集 Vol.9 No.-

Automatic fatigue crack length measuring system using DC electrical potential method and the system control program for automatic fatigue testing under random load condition were made in this study. And using these system and control program, fatigue tests were executed under constant and random load condition. As the result, the propagation of crack in random loading can be represented Paris equation and log normal probability function. But, constant and random load test show different crack propagation properties.

초등교육용 온라인 멀티미디어 코스웨어 개발을 위한 설계 전략

배종수,최성우 서울교육대학교 과학교육연구소 1997 科學과 數學敎育 論文集 Vol.23 No.-

Helicobacter pylori 항원을 이용한 면역우유 생산 및 항체특성에 관한 연구

배만종,김수정,예은주,김병기,박창호,김미경 경산대학교 생명자원개발연구소 2003 생명자원과 산업 Vol.7 No.-

본 연구는 위염, 위궤양, 위림프종 및 위암과 같은 소화기 질환의 원인균으로 알려진 Helicobacter pylori균을 항원으로 하여 젖소에 면역시킨 후 생산된 우유의 anti-H. pylorigkdcp의 생성능을 검토하고, 백신투여량과 항체 생성과의 관계, 항원 항체의 특이성, H. Pylori균 응집력, 항체의 산과 열에 대한 안정성, 그리고 백신투여가 젖소에 미치는 영향을 알아보았다. 1. 백신 투여량에 따른 혈청과 유청내의 anti-H. pylori항체의 함량은 10㎖, 20㎖, 30㎖ 백신투여 모든 군에서 대조구에 비해서 높은 양의 항체 생성을 확인하였다. 유의적인 차이는 없었으나 20㎖투여가 항체 생성이 가장 많은 것으로 조사되었다. 백신 투여량에 따른 유청내의 anti-H. pylori항체 생성량은 혈청에서 나타난 결과와 유사한 양상으로 형성되었다. 2. Anti-H. pylori항체의 SDS-PAGE에 의한 분자량측정 결과 heavy chain은 50kDa정도, light chain은 24kDa정도로 확인 되었다. 3. H. pylori항원 단백질의 분자량측정 결과 12개의 band가 형성되었다. Anti-H. pylori의 항원 특이성을 알아보기 위해 western bloting을 한 결과 혈청, 혈청정제, 유청, 유청 정제 모두 7개의 항원성 물질을 확인할 수 있었고, 주 항 원성 물질은 분자량이 97, 66, 34kDa 이었다. 4. 응집반응결과 유청속의 anti-H. pylori항체가 H. pylori균에 대해 1/10의 응집가를 나타내었다. 5. Anti-H. pylori항체의 산·알칼리에 대한 안정성 실험에서는 pH 5 ∼ pH 10 범위에서 안정한 상태로 100%의 활성을 나타내었다. 6. Anti-H. pylori항체의 열에 대한 안정성 실험에서는 60℃에서 60분간 안정한 상태를 보였고, 70℃에서도 비교적 안정한 상태였으나 60분 경과후 40%정도 활성이 감소하였다. 80℃에서는 4분간 처리했을 때 77%의 활성을 유지하였고, 100℃에서도 1분간은 비교적 안정한 상태였다. 7. 백신투요로 인하여 유량이 12% 감소하는 경향을 나타냈고, 최장 1주일 정도 지나면서 회복되었다. 8. 백신투여 후 젖소의 체온을 측정한 결과 대조구에 비해 정상적인 범위 내에서 체온이 상승하는 경향을 나타내었다. This study has been to examine bio-function of anti-H. pylori antibodies of milk produced from cows immune with antigen germ of Helicobacter pylori and search the relation between vaccine dosage volume and antibody formation, peculiarity of antigen antibody, cohesion of H. pylori germ, stability about add and heat of antibody, and impact of vaccine dosage on cows. The content of serum and Anti-H. pylori antibody within whey in accordance with vaccine dosage volume has confirmed the formation of high-quantified antibody compared to the controlled conditions in all groups vaccine dosages of 10㎖, 20㎖, and 30㎖. It has been turned out that the antibody was farmed most in 20㎖ dosage on while there was no attention difference. The molecular weight of Anti-H. pylori antibody measured by SDS-PAGE were turned out as about 50kDa in the heavy chain and about 24kDa in the light chain. 12 bands were formed as the result of measured molecular weight of antibody protein. The western blotting was performed in order to examine the antigen peculiarity of Anti-H. pylori that all 7 antigen substances including serum, serum refining, whey and whey refining could be confirmed and the main antigen substances were 97, 66, 34kDa of molecular weight. As a result of cohesive response Anti-H. pylori antibody in whey showed 1/10 cohesive rate about H. pylori germ. In stability test about acid and alkali of antibody there was 100% activated in the range of pH 5-pH 10. In stability test about heat it showed stable condition in 60℃: for 60 minutes and comparatively stable condition in 70℃, but reduced activation to 40% after 60 minutes. It maintained 77% activation in 80℃ for 4 minutes and comparatively stable in 100℃ for I minute. It was inclined to reduce to 12% of flow caused by vaccine injection, but recovered after about maximum 1 week. In measurement of body temperature of cows after vaccine injected, it was inclined to rise with the normal scope in comparison with the controlled conditions.

肝吸蟲에 대한 Albendazole의 殺蟲效果에 관한 實驗的 硏究

裵平元,金洙鎭,林漢鍾 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.3

Newly developed albendazole(zentel®) is a broad spectrum anthelmintic which high activity against intestinal nematodas as well as trematodes and cesstodes infections. The present study was undertaken to evaluato in vitro and in vivo activities of albendazoles against adult worms of clonorchis sinensis. The morphological change of C. sinensis obtained from experimentally infected rats treated with various dosages of albendazole were studied by means of scaning and transmission electron microscope. The results are as follows: 1. The adult worms of C. sinensis were incubated in the medium containing albendazole with various concentrations. By 24 hours after incubation, none of the worms were dead in concentrations of 0.01μg/ml of albendazole. 2. In order to evaluate the effect of albendazole in vivo, experimentally infected rats with 50 metacencariae of C. sinensis each were treated orally with various dosages of albendazole. In the result, anthelmintic effect was confirmed at the dosage of albendazole with 2×25㎎/㎏×2 days (total amounts 100㎎/㎏). 3. Macrocopital examination was to the clonorchis worms which were collected from the bile ducts of the rats autopsied at 24 hours after treatment with albendazole. The lack of intestinal contents was observed in most of worms from the groups treated with 1×200 ㎎/㎏ and 2×25 ㎎/㎏×2 or 4 days, and the elongation of the anterior portion of the worm body was also observed n the worms from the groups treated with 2×25 ㎎/㎏×2 or 4 days. 4. In the scanning electron microscopic observation, many blebs were fromed on the surface of the damaged tegument of the worms at 24 huors after treatment with albendazole(1×200 ㎎/㎏ and 2×25 ㎎/㎏×2 or days). 5. The transmissions electron microscopic observation indicated that degeneration of mitochonriae and destruction of tegumental syncytium of the worms at 24 hours after treatment with albendazole(1×200 ㎎/㎏ and 2×25㎎/㎏ or 4 days). Inaddition to the findings the degeneration of the intestinal lamellae in the caeca was also observed in the worms from the groups treated with same dosages of albendazole.

計算機를 活用하여 問題 解決 指導를 위한 資料 開發에 대한 硏究

배종수 서울교육대학교 과학교육연구소 1997 科學과 數學敎育 論文集 Vol.23 No.-

서울敎育大學敎 學內 電算網 構築 方案에 관한 硏究

裵鍾洙,洪命熹,張豪演 서울교육대학교 과학교육연구소 1994 科學과 數學敎育 論文集 Vol.20 No.-

數學 및 科學 敎育 授業現場에서의 Portfolio의 適用 : 基礎 硏究(Ⅰ) Preliminary Study(Ⅰ)

裵鐘洙,徐熹愛 서울교육대학교 과학교육연구소 1995 科學과 數學敎育 論文集 Vol.21 No.-

The term portfolio has become a popular word in areas of educational research. Unfortunately, the background of educational theories and philosophical perspectives has not been clearly established to support the effectiveness of the use of portfolios in classrooms. In this study, we have reviewed the definitions of portfolio, have analyzed the effectiveness of portfolio in mathematics and science education, and have discussed suggestions and guidelines for teachers how to use portfolios in their classroom. Further, from this study, we proposed that portfolio is grounded in learning process which aligned with Constructivism and individualized instruction. Learning characteristics of students in portfolio classroom include self-refelction upon their own learing, continuous growth in learning, and self-motivated learning. Students also improve academic, personal management, and teamwork skills in the process of learning. Particularly, the use of portfolio in science and mathematics classrooms enhance student's problem solving skill, creativity, reasoning skill, scientific thinking skill, and ability to apply knowledge to real world. It was recommended that teachers and teacher educators need to understand the nature of portfolio in order to effectively implement portfolios in classrooms. Changes in current classroom culture toward portfolio approach would stimulate more effective student learning and improve science and mathematics education in the country.

위에서 발생한 원발성 순수편평상피암 1예

이수형,배용목,권균홍,조용건,이현승,김규종 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

A case of pure squamous cell carcinoma of the stomach in a 41-year-old female is reported. Its pathogenesis is still unknown and epidermoid carcinomas of the stomach without a glandular cell component are a rare tumor described in only about 20 patients since 1905. In Korea, six patients have reported to date. In this case upper gastrointestinal endoscopy showed a large ulceroinfiltrating mass with marginal irregularities in the lower body of the stomach. Histologic finding of biopsy specimen was squamous cell carcinoma. Abdominal computerd CT showed metastatic invasions to the spleen, abdominal wall and small bowel loop.

마우스에서 애엽 약침액의 급성독성시험

임종국,김철호,조경희,손윤희,배만종,남경수 경산대학교 생명자원개발연구소 2000 생명자원과 산업 Vol.4 No.-

쑥 양침액의 투여와 관련된 변화를 관찰한 결과, 전 시험기간을 통해 단회투여 후 14일 동안 일반증상, 치사율, 체중변화, 부검소견, 장기중량변화, 혈청생화학적 분석등의 각종 지표에 의한 안전성을 평가했을 때 1× 실험군에서는 대조군에 비해 유의한 변화를 관찰할 수 없었으며 10×쑥약침액 투여 후 관찰된 체중의 감소, 혈정생화학적 수치의 유의한 변화는 과용량의 시험물질에 의한 영향으로 생각된다. 한편, 5×, 10×의 쑥약침액을 투여한 실험군에서 활동력 저하, 호흡수 감소, 졸음 및 무기력등의 임상증상이 관찰되었는데 이는 중추신경계 억압에 의한 증상으로 보고??되어 있다. 또한, 혈액내 혈청을 분리하여 간세포 이상의 지표로 사용하고 있는 GOT, GPT를 측정하였고 혈청 중의 total enzyme을 측정하여 LDH의 활성치를 계산, 이것을 심폐질환, 혈액질환, 간질환의 유무를 알아내는 지표로 사용하였으며 간염, 간경변, 골질환의 유무를 알아내는 지표로 사용하는 ALP-s를 측정하였다. 농도에 따른 쑥 약침액의 영향을 알아본 결과 쑥 약침액을 대조군에 비하여 단회투여시 1×의 농도에서는 대조군에 비해 유의한 차이를 나타내지 않았으나 5×, 10×의 농도의 실험군에서는 상당히 유의한 차이를 나타냄으로써 쑥 약침액의 과용량의 투여는 마우스의 간세포, 골세포, 심폐기관 등에 유해작용을 일으킬수 있음을 알 수 있었다. 본 실험을 통해 1×의 쑥 약침액의 용량은 급성 독성시험 결과 무해한 용량으로 판단되며, 5×이하의 용량군을 세밀히 나누어 검토할 필요가 있다고 판단된다. Acute toxicity of Artemisia Nakai Aqua-acupuncture Solution(ANAS) as studied in ICR mice. Mice were injected inrtapeitoneally with single dose of 1×, 5×, 10× ANAS and toxicological responeses were observed for consecutive 14 days. Mortality, clinical signs, body weight changes, organ weight, and serum chemistry were performed. The mortality and body weight changes of mice treated with 1× and 5× ANAS were not affected during the experimental periods. With the 10? ANAS treatment, dead animals and changes of body weight, organ weight and serum biochemical values were observed during the experimental period. There results suggest that 1× ANAS causes no toxicity that 10× ANAS causes toxicity in acute toxicity test.

Walsh함수 단일항전개에 의한 대규모 시스템의 비집중형 최적제어

安斗守,李漢錫,裵鍾一 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.1

This paper presents a method of decentralized optimal control for time invariant large scale systems via Single Term Walsh Series. In coordinator of decentralized optimal control, the interaction prediction principle approach of Takahara is used as the vehicle for large scale system. The main reason for this is that the prediction method require very little second-level calculation. In the subsystems of that, it is well known that the optimal control of a large scale system with quadratic performance criteria often involves the determination of time varying feedback gain matrix by solving the matrix Riccati differential equation, which is usually quite difficult. Therefore, in order to solve the problem, this paper is introduced to Single Term Walsh Series. The advantages of proposed method are simple and attractive for the control of large scale system in computation.