http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
허규희,허정식,김선욱,이영기,박덕배 제주대학교 생명과학기술혁신센터 2002 제주생명과학연구 Vol.5 No.-
Insulin has a variety of activities like as glucose uptake, inhibition of lipolysis, glycogen biosynthesis in a number of cells and tissues. Recently insulin has known to exert an antiapoptotic activity in different cell systems. However, it is not clear by which insulin can protect apoptosis cells from apoptosis. Thus, CHO cells expressing the human insulin receptors (CHO-IR) were used to evaluate the importances of different signaling steps activated by insulin in this process. The Ras/Raf-1/MEK/ERK cascade and the serine/threonine protein kinase Akt are two survival pathways that may be activated in response to insulin. We tested the hypothesis that inhibition of farnesylated Ras was casually related to manumycin-induced apoptosis and showed that the response to manumycin was found to be independent of K-Ras function. Moreover, blocking Ras/Raf-1/MEK/ERK cascade by the expression of a transdominant inhibitory mSOSI mutant in CHO-IR cells kept cells to sensitive to the antiapoptotic action of insulin. Insulin-dependent activation of Akt was blocked by 4th treatment with manumycin. This study suggest that the deplection of short-lived farnesylated proteins by manumycin suppresses the antiapoptotic action of insulin at least in part by disrupting Aktactivation but not of the K-Ras/Raf-1/MEK/ERK-dependent cascade.
HL-60 백혈병 세포의 성장에 대한 노랑하늘타리의 효과
김상철,박수영,허규희,김세재,강희경 제주대학교 생명과학연구소 2001 제주생명과학연구 Vol.4 No.-
The purpose of the present study is to examine the inhibitory effect of extracts of Trichosanthes kirilpwii var. japonica on the growth of HL-60 cells and to develop an anti-cancer agent using components of its leaves. To examine the inhibitory effect on the growth, metabolic activity was measured with 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay. In results, methanol(MeOH) extract of its leaves and ethylacetate (EtOAc) fraction of MeOH extract markedly inhibited the growth of these cells. At the same time, DNA fragmentation was observed in its extract-treated HL-60 cells. Therefore, inhibitory effect of Trichosanthes kirilpwii var. japonica on the growth of HL-60 seems to arise from the induction of apoptosis.