[문화 에세이] 달항아리 예찬

강희경,천성철(편집자) 한국콘크리트학회 2024 콘크리트학회지 Vol.36 No.2

Mesenchymal Stem Cells Ameliorate Adriamycin Induced Proteinuric Nephropathy

강희경,박소연,하일수,정해일,최용,Kang, Hee-Gyung,Park, So-Yeon,Ha, Il-Soo,Cheong, Hae-Il,Choi, Yong Korean Society of Pediatric Nephrology 2010 Childhood kidney diseases Vol.14 No.1

목 적 : 사구체신염은 흔히 단백뇨를 보이며 특이 치료법이 없고, 만성 신부전으로 발전하는 경우가 많다. 몇몇 연구에서 중간엽 줄기세포(Mesenchymal stem cell, MSC)를 실험적 사구체신염에 투여하여 단백뇨가 호전된 것을 보고한 바 있으나, 이는 신염을 일으키는 약제와 중간엽 줄기세포를 함께 투여하거나 신장에 직접 투여한 것이었다. 본 연구에서는 실험적 신병증에서 단백뇨가 발현된 시점에서 정주 요법으로 MSC를 투여함으로써 MSC의 임상적인 적용 가능성을 탐색하였다. 방 법 : 실험용 생쥐에 Adriamycin을 투여하여 신병증(ADR-GN)을 유발한 후, 2주 후에 대량의 단백뇨를 확인하고 MSC를 생쥐 꼬리의 정맥에 주사하였다. MSC에 의한 질병 완화의 기전을 확인하기 위한 in vitro 실험으로 mixed lymphocyte culture(MLC)에 MSC를 투여하였을 때의 염증 관련 cytokine인 IFN-$\gamma$ and IL-10의 변화를 측정하였다. 결 과 : 실험용 생쥐에 ADR-GN를 유발하고 단백뇨가 보일 때 MSC를 정주한 군에서는 단백뇨의 소실이 더 먼저 관찰되었다. 또한 MSC를 투여받은 군에서의 생존률이 더 나은 경향이 관찰되었다. MLC 에 MSC를 투여하였을 때, 염증을 유발하는 cytokine인 IFN-$\gamma$ 는 감소하고 염증을 억제하는 cytokine인 IL-10는 증가하였다. 결 론 : 이 연구는 이전의 보고들에서 관찰되었던 사구체신염에서의 MSC의 질병완화 효과가 좀더 임상적으로 적용 가능한 방법으로 투여된 경우, 즉 단백뇨가 있을 때 정주 요법으로 투여한 경우에도 관찰됨을 확인하였다. 이러한 효과의 기전과 임상적용에 요구되는 안전성 등에 대한 확인을 위해서는 추가 연구가 필요하겠다. Purpose : Glomerulonephropathy (GN) often manifests as proteinuria and progresses to chronic renal failure without specific therapy. Mesenchymal stem cell (MSC) has been tried as a therapeutic agent in experimental GN, and previous studies showed that administration of MSC concomitantly to the insult inducing GN or via intra-renal administration ameliorated proteinuria. The purpose of this study was to test the therapeutic potential of MSC administered via intravenous route at the time of clinically evident proteinuria. Methods : MSCs were administered intravenously via tail vain into the mice with adriamycin (ADR) induced nephropathy (ADR-GN), two weeks after ADR injection when massive proteinuria was evident. To test the capacity of MSC modulate the cytokine production in the inflammatory milieu, the concentrations of IFN-$\gamma$ and IL-10 were measured in the supernatant of in vitro mixed lymphocyte culture (MLC) with or without additional MSC. Results : MSCs administered intravenously into the proteinuric mice with ADR-GN accelerated the recovery of this experimental GN with disappearance of proteinuria in two weeks when the saline treated (control) mice still showed significant proteinuria. The mice treated with MSC also had a tendency of better survival. Addition of MSC decreased IFN-$\gamma$ and increased IL-10 in the supernatant of MLC. Conclusion : This study showed that MSC had a therapeutic potential even when administered in a more clinically relevant setting into a proteinuric glomerulonephropathy model. Further study to verify the mechanism and long-term safety of this phenomenon is required.

간호의 책임 : 빈곤 속의 아동 Childhood in poverty

강희경 제주한라대학 1997 論文集 Vol.21 No.-

Definition of poverty as a modifiable health risk is crucial to nursing, as nursing is committed to protecting and improving the health of disadvantaged populations. The theme of this article is "the health of childhood in poverty as a disadvantaged population". Since most of society's policies and programs are ostensibly based on research findings, the clarity, or its lack, in nursing's choice is critical in health care for poor children. For the theme of this article, work of Jurgen Habermas, the most prolific contemporary critical theorist, was briefly reviewed. In Harbermas' eyes, Poverty is a process that have dehumanized children. And, this process is a need to move beyond consideration of poverty as a variable to be controlled and to examine the relationship between poverty and health more carefully. Suggestions to enhance further investigation of this relationship include · Identification and clarification of the conceptual frameworks guiding inquiry into the relationship between health and poverty in childhood · Broadening the definitions of poverty providing operationalization · Developing the ideas about programs that can be integrated and translated into policy for children in poverty Conclusively, nursing is described as extending beyond the health care system and into the broader environment to include poverty affecting health. Poverty, therefore, is a modifiable health risk that can be considered within the scope of nursing practice.

간호진단과 컴퓨터 : 간호교육의 중심 테마 The Central Theme in Nursing Education

강희경 제주한라대학 1993 論文集 Vol.17 No.-

In this article, nursing diagnosis and computer assumed as the central theme in nursing education were reviewed. These are buildings as skills of survivals for nursing within other disciplines and guides for the domain of nursing within health care system. First, the view is taken that nursing diagnoses provide a way of conceptualizing the domain of nursing and a focus for theory development. Nursing diagnosis appear to be catching the attention of many in the nursing arenas of practice, administration, research, and education. Second, history and future of the computer in nursing education were reviewed. Nursing educators are beginning to realize that computer literacy has become a survival skill for the profession. They suggest the openness and awareness for future technologic possibilities. Also, the evolution of formal coursework in computer literacy has moved from learning "about the computer" to learning "with the computer". Today the use of the computer is expanding geometrically as microcomputers become common. Graduate students and faculty use them for literature searchs and data analysis. Undergraduates are routinely using computer-assisted instruction. Coursework in computer technology is fast becoming a given for nursing students and computer competency a requisite for faculty. Therefore, computer literacy becomes a necessity "for Heaven and the future's sake". Finally, the computer strategies for teaching nursing diagnosis were introduced. Computer techniques such as EXTEND (EXperT System for Education in Nursing Diagnosis) and ART (Adaptive Resonance Theory)-2 Neural Network et al. can provide new avenues for students and educators in nursing.

학령기 아동의 사회적 지지와 자기평가

강희경 한국부모교육학회 2011 부모교육연구 Vol.8 No.1

This study investigated the relationship between social supports and the self-evaluation of school-aged children. The Social Supports Scale is composed of three factors : social supports from family, friends and teachers. The subjects were 397 school-aged children. The main results were as follows. First, the level of social supports was generally high, with the highest level being from family. Second, the level of self-evaluation was also generally high. Finally, all supports from family, friends and teacher had positive influences on self-evaluation. The most influential factor was social support from friends. The implications of these results were discussed.

황순원 장편소설의 인본주의 성격 - 『나무들 비탈에 서다』,『일월』을 중심으로

강희경 국제한인문학회 2018 국제한인문학연구 Vol.- No.22

토양으로부터 Cryptococcus sp. CS-2의 분리 및 균주가 분비하는 Polygalacturonase의 특성에 관한 연구

강희경,문명님,임채영,양영기 한국미생물학회 1999 미생물학회지 Vol.35 No.2

제주도 밀감 과수원의 토양으로부터 polygalacturonase 고생산 효모를 분리하였다. 분리된 strain CS-2 의 생리화학적인 실험을 수행한 결과 분리균주는 Diazonium bule B color test 와 urease test에서 양성반응, acetic acid, citrate acid 생성 시험, gelatin 분해시험, fat 분해시험에서는 음성반응을 나타났고, 탄소원의 조사에서 galactose를 탄소원으로 이용할 수 없었고, 질소원은 모두 이용할 수 없었다. 또한 $30^{\circ}C$ 에서는 성장하는 반면, $35^{\circ}C$ 이상에서는 성장을 하지 못하였고 50% 이상의 glucose 농도, 0.01% cyclohexamide 그리고 1% acetic acid에서 성장을 하지 못하였다. 현미경을 이용한 형태학적 관찰 결과, 크기는 $1.3{\times}2.9$$\mu$m인 타원형으로 관찰되었다, ,multiple budding을 하며 ascospore는 존재하는 반면 pseudomycelium과 true mycelium 은 존재하지 않았다. 이와 같은 결과를 종합하여 Cryptococcus 속의 특징과 유사함을 알 수 있었다. 분리된 Cryptococcus sp. CS-2 의 polygalacturonase 는 유도적으로 합성되어 catabolic repression 에 의해 조절됨을 알 수 있었으며, 3일 배양시 가장 높은 활성도를 나타내었으며, 고유 활성도는 2.50~2.55 units/mg 으로 나타났다. 이러한 polygalacturonase를 SDS-PAGE, activity staining 그리고 단일 탄소원에 따른 단백질 양상의 변화를 비교하여 분자량 측정한 결과 약 46KDa으로 나타났다. A ploygalacturonase-produchg yeast was isolated from Cheju soil by selective eivichment media. One strain which has the highesl activity of polygalacturonase was selected. The characle~ishcs of the strain CS-2 were as follows: CS-2 utilized xylose. sucrose, maltose, u.ehalose, cellobiose. melibiose, lactose, raffinose, inosiiol, dulicilol, and dextrose, but did not utilized galactose, nitrate. nit~te, and lysine. Growth of CS-2 was inhibited by cyclohexamide, 1% acetic acid, and high concenaation (over 50%) of glucose. It grew at $30^{\circ}C$ but did 'IIOL $35^{\circ}C$. The cell size ofthe strain CS-2 was 2.9 p ~ n in length and 1.3 $\mu$ in diameter. Vegetable reproductmn was multiple budding and ascospre was present I to 4. Pseudomycelia or true myceliua formation were not observed In any of the cullureq. These results suggest that strain CS-2 is most likely a strain related Cryptococcus spp. (Cryptococcu spp. CS-2). When polygalacturonase or ihe yeast was induced by addition of polygalactoronic acid, polygalacturonase activity was detected in culture supernatent. There was a peak of specific activity a1 he mid-stationary phase(3 days culture) of growth. Polygalacturonase specific activity of Crylmcoccus sp. CS-2 was 2.96 unitsling. The molecular weighl ol'polygalacturonase was showed to be 46 KDa by both SDS-PAGE and activity stailling.

신이식 후 면역반응의 이해 2부 이식면역검사와 면역억제제

강희경,Kang, Hee-Gyung 대한소아신장학회 2008 Childhood kidney diseases Vol.12 No.2

이식을 위해서는 수여자와 공여자의 혈액형과 HLA type을 알아야 한다. 통상 ABO 혈액형이 적합한 경우 이식할 수 있으며 HLA 부적합은 근래 큰 문제가 되지 않으나 HLA 부적합이 없는 경우 이식장기의 장기생존률이 높다. PRA(panel reactive antibody)는 수여자가 HLA에 감작되었는지 검사하는 방법이며 이식 전에는 반드시 교차반응 검사를 하여 음성인 경우에만 이식을 진행한다. 이식 전후에 donor specific antibody(DSA)를 검사하여 이식장기에 대한 수여자의 면역반응을 예측 할 수 있다. 근래에는 스테로이드, calcineurin inhibitor(cyclosporine, tacrolimus), azathioprine 또는 mycophenolate mofetil (MMF)의 삼제요법을 주로 사용하며 항림프구 항체 (Thymoglobulin 또는 항IL-2 receptor 항체 basiliximab/daclizumab)을 이용하여 이식 초기에 면역억제상태를 induction하는 경우도 많다. For solid organ transplant, ABO blood type of donor and recipient should be compatible in principle. Recent improvement of immunosuppressant made HLA typing not so important while no-mismatch transplant still shows the longest graft survival. PRA(panel reactive antibody) test is to screen and identify recipients with HLA sensitization. When solid organ transplant is scheduled, cross-match test of donor cell and recipient serum should be performed and positive result of cross-match prohibits transplantation. Donor specific antibody(DSA) test can predict the severity of recipient immune reaction against donor organ. Today's mainstay of allograft immunosuppressant regimen is triple therapy of steroid, calcineurin inhibitor(cyclosporine, tacrolimus), azathioprine or mycophenolate mofetil(MMF). Antibody induction using Thymoglobulin or anti-IL-2 receptor antibody(basiliximab or daclizumab) is frequently practiced as well.

급성 골수성 백혈병 세포의 성장에 대한 비타민 C의 효과

강희경 제주대학교 생명과학연구소 1999 제주생명과학연구 Vol.2 No.-

The purpose of the present study is to examine the effect of vitamin C on the growth of leukemia cells. The effect was observed using KG-1 cell line which was derived from bone marrow of acute myelogenous leukemia patient. When KG-1 cells were grown in liquid media in the presence of vitamin C at the concentrations of 10-6 M to 10-3 M, the growth of these cells was stimulated up to 10-4 M and was inhibited at higher concentrations(≥ 5 X 10-4 M). The difference of growth according to the concentration of vitamin C was also observed in semisolid culture. The induction of differentiation on KG-1 cells by vitamin C was assessed by expression of cell surface antigens, CD 66b or CD 14 antigens. At low concentrations (10-6 to 10-4 M), vitamin C had little capacity to induce differentiation of KG-1 cells. In contrast, it was found that at higher concentrations(≥ 5 X 10-4 M) vitamin C-treated KG-1 cells showed activity to express CD 66b or CD 14 antigens. The results indicate that the degree of growth inhibition of KG-1 is in parallel with that of differentiation into granulocytes and monocytes. Therefore, inhibitory effect of vitamin C on the growth of KG-1 seems to rise from the induction of differentiation.

로바젯정으로 유발된 간수치 상승 환자의 한방 치료 증례 보고

강희경,조임학,이찬,허기윤,김민화,권정남,김소연,박성하,윤영주,이인,최준용,홍진우,한창우,Kang, Hee-kyung,Cho, Im-hak,Lee, Chan,Heo, Gi-yoon,Kim, Min-hwa,Kwon, Jung-nam,Kim, So-yeon,Park, Seong-ha,Yun, Young-ju,Lee, In,Choi, Jun-yong,Hong, 대한한방내과학회 2021 大韓韓方內科學會誌 Vol.42 No.5

Objective: This study evaluated a clinical case of a patient with Rovazet-induced abnormal liver function treated with intradermal acupuncture. Method: A 59-year-old woman with abnormal liver function induced by Rovazet ceased taking the drug and instead received intradermal acupuncture treatment. After 15 days, clinical symptoms and laboratory test results were evaluated. Results: Aspartate aminotransferase and alanine aminotransferase levels decreased, and clinical symptoms such as nausea and fatigue improved after treatment with intradermal acupuncture and cessation of the Rovazet. Conclusion: This study meaningfully examined a clinical case of a patient with abnormal liver function induced by a lipid-lowering agent, which is uncommon in Korea. Unfortunately, the lack of clear evidence and the use of only one case are limitations of the study.