호박벌 일벌독의 성분 분석 및 생리활성 탐색

한상미,이광길,여주홍,권해용,우순옥,윤형주,김미애,김원태,백하주,Han, Sang-Mi,Lee, Kwang-Gill,Yeo, Joo-Hong,Kweon, Hae-Yong,Woo, Soon-Ok,Yoon, Hyung-Joo,Kim, Me-Ae,Kim, Won-Tae,Baek, Ha-Ju 한국응용곤충학회 2007 한국응용곤충학회지 Vol.46 No.2

호박벌 일벌독의 성분과 생리활성을 규명하기 위하여 단백질 성분분석과 암세포 생육 저해 효능, 항균력을 검토하였다. 이차원단백질 분석을 통해 호박별의 일벌독은 63개의 단백질이 존재하는 것으로 확인되었으며, 가장 많은 함량을 보이는 3개의 단백질을 염기서열 분석하였다. 그러나 이들 성분은 아직 밝혀지지 않은 성분으로 판단되었다. 호박벌 일벌독의 암세포 (간암; Hep3B, 폐암; A549, 유방암; BT-20, 위암; AGS) 에 대한 생육 저해능은 시료 농도가 증가함에 따라 증가하는 경향을 보이며 100ng/ml에서 간암세포 (Hep3B) 에 대한 생육 저해능이 55%로 가장 높았다. 항균활성에 E. faecium 과 S. sonnei에 대하여 최소발육억제농도와 최소살균농도 모두 각각 0.256ug/ml 로 강한 항균활성을 나타내었으며, 그 외의 피검균에 대해서도 비교적 높은 활성을 보였다. 이상의 결과로부터 호박벌 일벌독의 성분은 다른 벌의 독성분과는 차이를 보이며, 그 생리활성에 있어 약리학적 이용이 가능할 것으로 판단되었다. Pure Bombus ignitus venom samples were submitted to two-dimensional gel electrophoresis. A total of 64 excised spots were analyzed by mass spectrometry. Three main proteins resulted in the identification have not been described in other bee venoms before. Dose-dependence against human carcinoma (Hep3B, BT-20, A549 and AGS) were observed from 1ng/ml to 100ng/ml. Expecially, the treatment of 100ng/ml B. ignitus venoms showed the highest cytotoxicity with 55% against hepatocellular carcinoma (Hep3B). The B. ignitus venoms showed strong antimicrobial activities against Enterococcus faecium and Shigella sonnei, and practically antimicrobial activity against the other microorganisms tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of E. faecium and S. sonnei, were 0.256ug/ml, respectively.

국산 프로폴리스의 Helicobacter pyrori 억제효과

한상미,홍인표,우순옥,김세건,장혜리,장재선,Han, Sang Mi,Hong, In Phyo,Woo, Soon Ok,Kim, Se Gun,Jang, Hye Ri,Jang, Jae Seon 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.1

Propolis possesses antimicrobial, anti-inflammatory, anaesthetic and immunostimulating activities. The aim of this study was to evaluated chemical composition and in vitro anti-Helicobacter pylori activity of eight propolis ethanolic extracts form different region in Korea. The chemical composition of eight Korean propolis was investicated by absorption spectrophotomety and UHPLC. Total penolic and total flavonoids content ranged between $21{\pm}1.5$ and $31{\pm}1.8$ gallic acid g/100 g and $7.2{\pm}0.9$ and $24.4{\pm}1.9$ quercetin g/100 g, respectively. The p-coumaric acid as standard components content ranged between $0.29{\pm}0.00$ and $2.34{\pm}0.02mg/g$ using UHPLC. Cinnamic acid content between $0.43{\pm}0.00$ and $1.33{\pm}0.01mg/g$. 80% ethanolic extract of propolis against H. pylori was evaluated by using the disc diffusion method. Ethanol was used as a control. Mean diameters of H. pylori growth inhibition using $10{\mu}g/mL$, $50{\mu}g/mL$ or $100{\mu}g/mL$ ethanol propolis per disc were 0, ranging from 0.9 to 2.1 mm and from 1.9 to 3.5 mm, respectively. In conclusion, variation in the chemical composition of Korean propolis were mainly due to the difference in the preferred regional plants visited by honeybees. Also Korean propolis possesses considerable antibacterial activity against H. pylori. There was not confirmed a correlation between the chemical composion contens and anti-H. pylori effect. The potential of propolis in the prevention or treatment of H. pylori infection is worth further extensive evaluation.

채취 시기 및 지역에 따른 봉독의 성분 분석

한상미,윤형주,백하주,Han, Sang-Mi,Yoon, Hyung-Joo,Baek, Ha-Ju 한국응용곤충학회 2012 한국응용곤충학회지 Vol.51 No.3

서양종 꿀벌 독의 채집시기와 지역에 따른 봉독의 성분 변화 및 약리효과에 미치는 영향을 검토하였다. 채집시기는 5월부터 9월까지, 채집지역은 전국 35개 지역으로부터 채취한 봉독을 대상으로 2010년과 2011년 2년에 걸쳐 동일 지역에서 동일한 방법으로 봉독을 채취하였다. 채취한 봉독은 액체크로마토그래피를 통해 멜리틴과 아파민 그리고 포스포리파아제 A2의 성분 함량을 분석하였다. 그 결과 채집시기와 지역에 따른 성분에 유의한 차이는 확인되지 않았다(One way-ANOVA, Duncan's test (${\alpha}$=0.05)). 봉독의 성분은 채집시기와 지역에 관계없이 멜리틴 $55.2{\pm}2.07%$, 아파민 $2.57{\pm}0.103%$ 그리고 포스포리파아제 A2는 $12.51{\pm}0.37%$을 차지하였다. 이상의 결과로부터 봉독은 채취시기에 따른 주요 성분은 차이를 갖고 있지 않았으며, 이는 꿀벌의 먹이, 사육온도 등 외부 환경이 봉독 분비에 영향을 주지 않는 것으로 사료되었다. This study aims to investigate whether geographical variation affects the antibacterial component properties of honeybee (Apis mellifera L.) venom in Korea. Honeybee venom samples were collected from May to September, during 2010 and 2011, from 35 different sites, and were analyzed for major components, including melittin, apamin and phospholipase A2 were determined by a liquid chromatography using ammonium formate, acetonitrile, trifluoracetic acid. On average, melittin, apamin and phospholipase A2 were determined $55.2{\pm}2.07%$, $22.57{\pm}0.103%$, and $12.51{\pm}0.37%$, respectively. The ratio of the major components, including melittin, apamin and phospholipase A2 did not differ significantly according to flower or temperature during collections (One way-ANOVA, Duncan's test (${\alpha}$=0.05)).

무당벌레(Harmonia axyridis) 추출물에 의한 BV-2 세포주의 Nitric Oxide 생성 저해 활성

한상미,이상한,윤치영,강석우,이광길,김익수,윤은영,이평재,김선여,황재삼,Han Sang-Mi,Lee Sang-Han,Yun Chi-Young,Kang Seok-Woo,Lee Kyung-Gill,Kim Ik-Soo,Yun Eun-Young,Lee Pyeong-Jae,Kim Sun-Yeou,Hwang Jae-Sam 한국응용곤충학회 2006 한국응용곤충학회지 Vol.45 No.1

퇴행성 뇌질환은 뇌에 존재하는 면역세포인 소교세포의 염증반응이 발병 요인 중의 하나로 알려져 있다. 이에 본 연구는 초고속자동화시스템(high throughput screening: HTS)을 이용하여 약용곤충추출물로부터 항산화와 항염증 기능이 있다고 알려진 무당벌레 추출물로부터 염증발생인자인 nitric oxide의 생성에 어떠한 영향을 주는지 다음과 같은 결과를 얻었다. 소교세포인 BV-2세포에 대한 무당벌레 추출물의 세포독성은 물과 메탄올, DMSO 추출물에서는 100 ng/ml 까지는 거의 없었으나 에탄올 추출물은 1 ng/ml에서도 세포독성이 있었다. 물과 메탄을 추출물(50 ng/ml)은 LPS로 활성화된 BV-2세포에서 $TNF-{\alpha}$와 $IL-1{\beta}$의 발생을 35-60% 가량 억제 하였다. LPS로 유도된 NO의 생성은 물과 메탄올 추출물을 처리했을 때 각각 55%, 76% 억제되었다. 또한, MeOH 추출물을 처리했을 경우 LPS에 의한 iNOS 발현 정도를 단백질 수준과 mRNA 수준에서 현저하게 억제시킴을 확인하였다. Inflammation in the brain has known to be associated with the development of a various neurologiacal diseases. The hallmark of neuro-inflammation is the activation of microglia, brain macrophage. Pro-inflammatory compounds including nitric oxide(NO) are the main cause of neuro-degenerative disease such as Alzheimer's disease. In the study, we examined whether Harmonia axyridis extracts inhibit the NO production by a direct method using Griess reagent, western blotting and by RT-PCR(Reverse Transcription-Polymerase Chain Reactionin) the gene expression of inducible nitric oxide synthase(iNOS). Distilled water$(H_2O)$ and methanol(MeOH) extracts of H. axyridis inhibited the protein expression of TNF-a(Tumor Necrosis Factor) and IL-6(Interleukin) in LPS (Lipopolysaccharide) stimulated BV-2 cells at the concentration of 100 ng/ml. Incubation of BV-2 cells with the extracts of $H_2O$ of MeOH inhibited the LPS induced NO and iNOS protein. And this inhibition of iNOS protein is concordant with the inhibition of iNOS mRNA expression. These data suggested that H. axyridis extracts may play a crucial role in inhibiting the NO production.

의류제품의 치수 규격동향

한상미,Han, Sang-Mi 기술표준원 2003 기술표준 Vol.15 No.-

사회복지행정론 교과목에 플립드러닝 적용 효과분석

한상미(Sang-mee Han) 한국사회복지교육협의회 2023 한국사회복지교육 Vol.63 No.-

지역사회복지관 사회복지사들의 사회자본 유형 연구

한상미 ( Sang Mee Han ) 한국사회복지정책학회 2007 사회복지정책 Vol.31 No.-

유아교사의 직무스트레스와 교직적응과의 관계에서 직업정체성의 매개효과

한상미 ( Han Sang-mi ),서현아 ( Seo Hyun-ah ) 한국유아교육학회 2020 유아교육연구 Vol.40 No.6

본 연구의 목적은 유아교사의 직무스트레스와 교직적응과의 관계에서 직업정체성의 매개효과를 알아봄으로써 유아교사의 교직적응을 향상 시킬 수 있는 방안을 모색하는 데 있다. 이를 위해 B시, G도에 소재한 유아교육기관의 유아교사 386명을 대상으로 직무스트레스, 교직적응, 직업정체성 척도로 설문조사를 실시하였으며, 자료분석은 Pearson 적률상관분석과 회귀분석 및 Sobel test를 통하여 응답결과를 분석하였다. 그 결과 첫째, 유아교사의 직무스트레스는 직업정체성과 교직적응에 부적 상관관계가 나타났으며, 직업정체성은 교직적응에 정적 상관관계가 나타났다. 또한 유아교사의 직무스트레스는 교직적응과 직업정체성을 낮추는 부정적인 영향을 미치며, 직업정체성은 교직적응을 높이는 긍정적인 영향을 미치는 것으로 나타났다. 둘째, 유아교사의 직무스트레스와 교직적응과의 관계에서 직무스트레스는 직업정체성을 부분 매개하여 교직적응에 영향을 미치는 것으로 나타났다. 따라서 유아교사의 직무스트레스를 줄일 수 있는 방안을 모색하고 유아교사의 직업정체성을 높여줌으로써 교직적응에 긍정적인 영향을 줄 수 있음을 시사한다. The purpose of this study was to provide ways of improving early childhood teachers’ adjustment to the teaching profession by investigating the mediating effect of professional identity on relationships between those teachers’ job stress and adjustment to that profession. A questionnaire survey was constructed of 386 teachers working in early childhood education institutions located in Busan and Gyeongnam Province. The survey was conducted employing scales respectively for job stress, adjustment to the teaching profession and professional identity. Data from the surveyed teachers’ responses was analyzed through Pearson’s product-moment correlation coefficient analysis, regression analysis and the Sobel test. The findings are summarized as follows. First, there were negative correlations between early childhood teachers’ job stress and professional identity or adjustment to the teaching profession, In contrast, negative correlations existed between their professional identity and adjustment to the teaching profession. Second, in case of relationships between early childhood teachers’ job stress and adjustment to the teaching profession, job stress was shown to have an effect on the adjustment through a partial mediation of professional identity. These findings suggest that effective ways to reduce early childhood teachers’ job stress should be developed and implemented in order to foster the teachers’ professional identity, which would have a positive effect on their adjustment to the teaching profession.

젖소 유방염에 대한 국내산 봉독의 치료 효과

한상미 ( Sang Mi Han ),이광길 ( Kwang Gill Lee ),여주홍 ( Joo Hong Yeo ),권해용 ( Hae Yong Kweon ),우순옥 ( Soon Ok Woo ),오백영 ( Baeg Young Oh ),이윤근 ( Yun Geun Lee ),김봉순 ( Bong Soon Kim ),백하주 ( Ha Ju Baek ),김순태 ( Soo 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1

The therapeutic effect of honeybee venom collected using bee venom collector on bovine mastitis was investigated. Mastitic cows from four farms were selected in the Yang-pyeong areas. Chronic mastitic cows were injected with the various concentrations of honey-bee venom per day. There was a significant difference in the reduction rates of somatic cell counts (SCC) according to treatment concentration and method of bee venom. The milk SCC were significantly decreased in all concentrations of bee venom 3 days after treatment. The reduction rates of SCC in treatment of 3, 6, 12 and 24㎎ honeybee venom were 20, 43, 63.3 and 65.8% respectively. Honeybee venom treatment consisted of two methods, a syringeful and a Bovivet Spenstift . The treatment with Bovivet Spenstift was more effective in the reduction rates of SCC compared with the syringeful. Thirty two out of 53 quarters were cured by Bovivet Spenstift with 12㎎ bee venom per day for 14 days. The venom cure rates of bovine mastitis by Escherichia coli, Stapylococcus aureus, Gram positive bacteria and Gram negative bacteria were 33.3, 75, 75 and 43.8% respectively. These results suggested that bee venom treatment (by Bovivet Spenstift with 12㎎) might be effective for treatment of bovine mastitis.

온라인 한국어 교육과정 개발을 위한 기초 연구

한상미 ( Sang Mee Han ),김제열 ( Je Yeol Kim ),전지인 ( Jee In Chun ),이수민 ( Su Min Lee ),최유경 ( Yoo Kyung Choi ),김종인 ( Jong In Kim ),김성진 ( Seong Jin Kim ),김지현 ( Chee Hyun Kim ),김미혜 ( Mi Hye Kim ),김지원 ( Ji Won K 연세대학교 언어연구교육원 한국어학당 2012 외국어로서의 한국어교육 Vol.37 No.-

A Research on the development of the online Korean Curriculum. Teaching Korean as a Foreign Language, 37, 411~448. This is a research on the development of the online Korean curriculum of the Korean Language Institute(KLI), Yonsei University, focusing on the educational contents comprising the KLI online Korean program and the principle of LMS development. In chapter two, we examined the concepts of e-learning, e-learning 2.0 and smart-learning, and the characteristics of e-learning activities. In chapter three, the principles of developing online contents for Korean language education were investigated by holistically examining the content composition along with a model unit. In chapter four, the principles of LMS development were demonstrated and plans for program implementation were suggested. Finally, in chapter five, we summarized our study and established and explained its significance.