무당벌레(Harmonia axyridis) 추출물에 의한 BV-2 세포주의 Nitric Oxide 생성 저해 활성

한상미,이상한,윤치영,강석우,이광길,김익수,윤은영,이평재,김선여,황재삼,Han Sang-Mi,Lee Sang-Han,Yun Chi-Young,Kang Seok-Woo,Lee Kyung-Gill,Kim Ik-Soo,Yun Eun-Young,Lee Pyeong-Jae,Kim Sun-Yeou,Hwang Jae-Sam 한국응용곤충학회 2006 한국응용곤충학회지 Vol.45 No.1

퇴행성 뇌질환은 뇌에 존재하는 면역세포인 소교세포의 염증반응이 발병 요인 중의 하나로 알려져 있다. 이에 본 연구는 초고속자동화시스템(high throughput screening: HTS)을 이용하여 약용곤충추출물로부터 항산화와 항염증 기능이 있다고 알려진 무당벌레 추출물로부터 염증발생인자인 nitric oxide의 생성에 어떠한 영향을 주는지 다음과 같은 결과를 얻었다. 소교세포인 BV-2세포에 대한 무당벌레 추출물의 세포독성은 물과 메탄올, DMSO 추출물에서는 100 ng/ml 까지는 거의 없었으나 에탄올 추출물은 1 ng/ml에서도 세포독성이 있었다. 물과 메탄을 추출물(50 ng/ml)은 LPS로 활성화된 BV-2세포에서 $TNF-{\alpha}$와 $IL-1{\beta}$의 발생을 35-60% 가량 억제 하였다. LPS로 유도된 NO의 생성은 물과 메탄올 추출물을 처리했을 때 각각 55%, 76% 억제되었다. 또한, MeOH 추출물을 처리했을 경우 LPS에 의한 iNOS 발현 정도를 단백질 수준과 mRNA 수준에서 현저하게 억제시킴을 확인하였다. Inflammation in the brain has known to be associated with the development of a various neurologiacal diseases. The hallmark of neuro-inflammation is the activation of microglia, brain macrophage. Pro-inflammatory compounds including nitric oxide(NO) are the main cause of neuro-degenerative disease such as Alzheimer's disease. In the study, we examined whether Harmonia axyridis extracts inhibit the NO production by a direct method using Griess reagent, western blotting and by RT-PCR(Reverse Transcription-Polymerase Chain Reactionin) the gene expression of inducible nitric oxide synthase(iNOS). Distilled water$(H_2O)$ and methanol(MeOH) extracts of H. axyridis inhibited the protein expression of TNF-a(Tumor Necrosis Factor) and IL-6(Interleukin) in LPS (Lipopolysaccharide) stimulated BV-2 cells at the concentration of 100 ng/ml. Incubation of BV-2 cells with the extracts of $H_2O$ of MeOH inhibited the LPS induced NO and iNOS protein. And this inhibition of iNOS protein is concordant with the inhibition of iNOS mRNA expression. These data suggested that H. axyridis extracts may play a crucial role in inhibiting the NO production.

의류제품의 치수 규격동향

한상미,Han, Sang-Mi 기술표준원 2003 기술표준 Vol.15 No.-

호박벌 일벌독의 성분 분석 및 생리활성 탐색

한상미,이광길,여주홍,권해용,우순옥,윤형주,김미애,김원태,백하주,Han, Sang-Mi,Lee, Kwang-Gill,Yeo, Joo-Hong,Kweon, Hae-Yong,Woo, Soon-Ok,Yoon, Hyung-Joo,Kim, Me-Ae,Kim, Won-Tae,Baek, Ha-Ju 한국응용곤충학회 2007 한국응용곤충학회지 Vol.46 No.2

호박벌 일벌독의 성분과 생리활성을 규명하기 위하여 단백질 성분분석과 암세포 생육 저해 효능, 항균력을 검토하였다. 이차원단백질 분석을 통해 호박별의 일벌독은 63개의 단백질이 존재하는 것으로 확인되었으며, 가장 많은 함량을 보이는 3개의 단백질을 염기서열 분석하였다. 그러나 이들 성분은 아직 밝혀지지 않은 성분으로 판단되었다. 호박벌 일벌독의 암세포 (간암; Hep3B, 폐암; A549, 유방암; BT-20, 위암; AGS) 에 대한 생육 저해능은 시료 농도가 증가함에 따라 증가하는 경향을 보이며 100ng/ml에서 간암세포 (Hep3B) 에 대한 생육 저해능이 55%로 가장 높았다. 항균활성에 E. faecium 과 S. sonnei에 대하여 최소발육억제농도와 최소살균농도 모두 각각 0.256ug/ml 로 강한 항균활성을 나타내었으며, 그 외의 피검균에 대해서도 비교적 높은 활성을 보였다. 이상의 결과로부터 호박벌 일벌독의 성분은 다른 벌의 독성분과는 차이를 보이며, 그 생리활성에 있어 약리학적 이용이 가능할 것으로 판단되었다. Pure Bombus ignitus venom samples were submitted to two-dimensional gel electrophoresis. A total of 64 excised spots were analyzed by mass spectrometry. Three main proteins resulted in the identification have not been described in other bee venoms before. Dose-dependence against human carcinoma (Hep3B, BT-20, A549 and AGS) were observed from 1ng/ml to 100ng/ml. Expecially, the treatment of 100ng/ml B. ignitus venoms showed the highest cytotoxicity with 55% against hepatocellular carcinoma (Hep3B). The B. ignitus venoms showed strong antimicrobial activities against Enterococcus faecium and Shigella sonnei, and practically antimicrobial activity against the other microorganisms tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of E. faecium and S. sonnei, were 0.256ug/ml, respectively.

국산 프로폴리스의 Helicobacter pyrori 억제효과

한상미,홍인표,우순옥,김세건,장혜리,장재선,Han, Sang Mi,Hong, In Phyo,Woo, Soon Ok,Kim, Se Gun,Jang, Hye Ri,Jang, Jae Seon 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.1

Propolis possesses antimicrobial, anti-inflammatory, anaesthetic and immunostimulating activities. The aim of this study was to evaluated chemical composition and in vitro anti-Helicobacter pylori activity of eight propolis ethanolic extracts form different region in Korea. The chemical composition of eight Korean propolis was investicated by absorption spectrophotomety and UHPLC. Total penolic and total flavonoids content ranged between $21{\pm}1.5$ and $31{\pm}1.8$ gallic acid g/100 g and $7.2{\pm}0.9$ and $24.4{\pm}1.9$ quercetin g/100 g, respectively. The p-coumaric acid as standard components content ranged between $0.29{\pm}0.00$ and $2.34{\pm}0.02mg/g$ using UHPLC. Cinnamic acid content between $0.43{\pm}0.00$ and $1.33{\pm}0.01mg/g$. 80% ethanolic extract of propolis against H. pylori was evaluated by using the disc diffusion method. Ethanol was used as a control. Mean diameters of H. pylori growth inhibition using $10{\mu}g/mL$, $50{\mu}g/mL$ or $100{\mu}g/mL$ ethanol propolis per disc were 0, ranging from 0.9 to 2.1 mm and from 1.9 to 3.5 mm, respectively. In conclusion, variation in the chemical composition of Korean propolis were mainly due to the difference in the preferred regional plants visited by honeybees. Also Korean propolis possesses considerable antibacterial activity against H. pylori. There was not confirmed a correlation between the chemical composion contens and anti-H. pylori effect. The potential of propolis in the prevention or treatment of H. pylori infection is worth further extensive evaluation.

미국자리공(Phytolacca americana L.)의 뿌리에서 추출한 α-spinasterol의 생물활성 검정

한상미,배기환,최관삼,Han, Sang Mi,Bae, Ki Hwan,Choi, Kwan Sam 충남대학교 농업과학연구소 1996 Korean Journal of Agricultural Science Vol.23 No.2

미국자리공의 뿌리로 부터 추출한 분획물에 대한 생물활성을 관찰하였다. 그 중 에틸아세테이트 추출 분획물에서 분리한 $\alpha$-spinasterol가 식물의 초기생육에 있어서는 $10{\mu}g/ml$의 낮은 농도에서도 뿌리의 괴사를 유발시킴으로서 식물체 전체에 대한 고사까지 초래하였다 미생물에 대한 생장 억제 효과로서는 Mucor racemosus에 대하여는 그 값이 $200{\mu}g/disk$ 정도로 높은 억제력을 보였다. 그러나, 두종류의 공시된 동물 암세포에 대한 세포독성은 미약하였다.

채취 시기 및 지역에 따른 봉독의 성분 분석

한상미,윤형주,백하주,Han, Sang-Mi,Yoon, Hyung-Joo,Baek, Ha-Ju 한국응용곤충학회 2012 한국응용곤충학회지 Vol.51 No.3

서양종 꿀벌 독의 채집시기와 지역에 따른 봉독의 성분 변화 및 약리효과에 미치는 영향을 검토하였다. 채집시기는 5월부터 9월까지, 채집지역은 전국 35개 지역으로부터 채취한 봉독을 대상으로 2010년과 2011년 2년에 걸쳐 동일 지역에서 동일한 방법으로 봉독을 채취하였다. 채취한 봉독은 액체크로마토그래피를 통해 멜리틴과 아파민 그리고 포스포리파아제 A2의 성분 함량을 분석하였다. 그 결과 채집시기와 지역에 따른 성분에 유의한 차이는 확인되지 않았다(One way-ANOVA, Duncan's test (${\alpha}$=0.05)). 봉독의 성분은 채집시기와 지역에 관계없이 멜리틴 $55.2{\pm}2.07%$, 아파민 $2.57{\pm}0.103%$ 그리고 포스포리파아제 A2는 $12.51{\pm}0.37%$을 차지하였다. 이상의 결과로부터 봉독은 채취시기에 따른 주요 성분은 차이를 갖고 있지 않았으며, 이는 꿀벌의 먹이, 사육온도 등 외부 환경이 봉독 분비에 영향을 주지 않는 것으로 사료되었다. This study aims to investigate whether geographical variation affects the antibacterial component properties of honeybee (Apis mellifera L.) venom in Korea. Honeybee venom samples were collected from May to September, during 2010 and 2011, from 35 different sites, and were analyzed for major components, including melittin, apamin and phospholipase A2 were determined by a liquid chromatography using ammonium formate, acetonitrile, trifluoracetic acid. On average, melittin, apamin and phospholipase A2 were determined $55.2{\pm}2.07%$, $22.57{\pm}0.103%$, and $12.51{\pm}0.37%$, respectively. The ratio of the major components, including melittin, apamin and phospholipase A2 did not differ significantly according to flower or temperature during collections (One way-ANOVA, Duncan's test (${\alpha}$=0.05)).

천연항생제로서 봉독의 기원 및 지표성분 설정

한상미 ( Sang Mi Han ),김정민 ( Jung Min Kim ),한상훈 ( Sang Hoon Han ),여주홍 ( Joo Hong Yeo ),홍인표 ( In Pyo Hong ),우순옥 ( Soon Ok Woo ),이광길 ( Kwang Gill Lee ),권해용 ( Haeyong Kweon ) 한국동물위생학회 2014 한국동물위생학회지 (KOJVS) Vol.37 No.2

This research was performed in order to investigate the origin, standard compound, and structural and physical properties of honeybee venom which used as natural antibiotic ingredients to animal. We compared the nucleotide sequence of mitochondrial cytochrome c oxidase subunit 1 gene (COI) of honeybees were collected from Gangwon, Gyeonggi, Chungnam, Gyeongbuk, Gyeongnam province and Suwon. As major constituent of honeybee venom, melittin was assayed by liquid chromatography. X-ray, differential scanning calorimetry (DSC) and fourier transform infrared spectroscopy (FT-IR) were utilized to examine the structural and physical properties of honeybee venom. Based on the 627bp sequence of COI, Apis mellifera ligustica was determinated honeybees collected from all six regions. Melittin content varied from 50.7 to 68.6 and averaged 59.8%. According to XRD analysis, honeybee venom showed regular crystal structure peaks at 2θ=8.5° and 21.5°. DSC showed that the maximum degration temperature of powder was around 230°C. Through FT-IR analysis, we could identify cross-linking by the presence of peptide peak at 1,500∼1,600 cm-1. In conclusion, the origin of honeybee venom was Apis mellifera ligustica and effective ingredient standards was melittin content varied from 50.7 to 68.6 as natural antibiotic ingredients.

중등 가정과 교원양성을 위한 의생활 영역 교육과정에 대한 연구

한상미(Han Sang Mi),김경민(Kim, Kyungmin),이윤정(Lee, Yoon-jung) 학습자중심교과교육학회 2019 학습자중심교과교육연구 Vol.19 No.23

본 연구의 목적은 교육과정평가원(2008)에서 제시하는 가정과 의생활 분야 임용시험의 평가영역 및 내용요소가 교원양성기관에서 시행하는 의생활 분야의 교육과정을 평가하는 기준으로 적절한지 살펴보고, 이를 기준으로 사범대학 및 일반/교육 대학원의 의생활 분야 교육과정을 분석하는 데 있다. 우선, 27명의 전문가 집단에게의생활 분야 임용시험의 평가영역 및 내용요소의 적절성 및 필요성을 확인하였고, 2015 개정 교육과정과의 비교를 통해 다시 한 번 적절성을 확인하였다. 다음으로, 전 국 교원양성기관의 의생활 분야 교육과정 운영 현황 및 실태와 평가기준 반영 여부를 살펴보았다. 연구결과는 다음과 같다. 첫째, 교육과정평가원(2008)에서 제시하는 가정과 의생활 분야의 평가영역 및 내용요소는 교원양성기관의 교육과정을 평가하 기에 적절하고 필요한 내용이 포함되어 있는 것으로 나타났으며, 이는 2015 개정 교육과정과도 관련이 있는 것으로 나타났다. 둘째, 의생활분야 교육과정 운영실태를 살펴본 결과 사범대학에서는 의류학 분야 전공과목이 다수 운영되고 있으며 그 중 1~2개의 과목을 전공필수로 지정하고 있었지만, 교육대학원의 경우 전공필수로 1개과목만을 이수하도록 구성된 경우가 많았다. 그리고 대부분의 기관에서는 각 평가내용요소를 다양한 과목을 통해 다루고 있으나, 일부 학교에서는 의복과 환경에 관련 된 교과목이 없는 것으로 나타났다. 이상의 결과를 토대로 가정과 교원양성기관의 교육과정에 대해 논의하였다. The purpose of this study was to analyze ‘Clothing life’ curriculum on teacher preparation institutions for middle school home economics teachers based on the evaluation standard and contents on the secondary school home economics teacher certification examination. First, we examined propriety of the evaluation standard and contents on clothing life by survey of 27 home economics experts. Also, we compared the evaluation standard and contents on clothing life with 2015 revised curriculum. Second, we investigated ‘clothing life’ curriculum on teacher preparation institutions. Most college of education offered mandatory subjects relating clothing life, however, a few subject was administered at graduate school of education. And correlation between most institutions ‘clothing life’ curriculum and the evaluation standard and contents was high overall, except on ‘clothing and environment’. Suggestions for the ‘clothing life’ curriculum on teacher preparation institutions were discussed.

요양병원 간호사의 의사소통 능력과 직무스트레스가 조직유효성에 미치는 영향

한상미(Han Sang-Mi),박성주(Park Sung-Ju) 전남대학교 간호과학연구소 2019 Nursing and Health Issues(NHI) Vol.24 No.2

Purpose: This study was a descriptive survey and aimed to identify the communication skill, job stress, and organizational effectiveness of geriatric hospital nurses and examine the effects of communication skill and job stress on organizational effectiveness. Methods: The participants were 180 nurses with more than one year of work experience as a geriatric hospital nurse, who were randomly recruited from six selected long-term care hospitals with more than 100 beds, located in G Metropolitan City and J Province. Results: Communication skill had an average score of 3.56 points, job stress had 3.77 points, and organizational effectiveness had 3.17 points on a five-point scale. Organizational effectiveness of long-term care hospital nurses had a significant positive correlation with communication skill (r=.43, p <.001) and a significant negative correlation with job stress (r=−.28, p <.001). The factors significantly influencing organizational effectiveness were communication skill and job stress. The explanatory power of the two variables was 23%. Conclusion: Organizational effectiveness had a significant positive correlation with communication skill and a significant negative correlation with job stress. Therefore, it is judged to be necessary to develop various studies and programs for enhancing communication skill and reducing job stress in order to promote organizational effectiveness of long-term care hospital nurses.

제주산 감귤꿀과 유채꿀의 항산화 및 멜라닌 생성 억제 효과

한상미(Sang Mi Han),우순옥(Soon Ok Woo),남성희(Sung Hee Nam),박서희(Seo Hui Park) 한국양봉학회 2011 Journal of Apiculture Vol.26 No.2

In order to investigate the potential of Korean honey for whitening cosmetics, we prepared citrus blossom and oilseed rape honey from Jeju island in Korea. Citrus blossom and oilseed rape honey were showed antioxidant activities, such as 1,1-diphenyl-2-pycrylhydrazyl (DPPH) (IC<SUB>50</SUB>=29.25 and 41.5㎍/mL, respectivity) and 2,2′-azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) (IC50=38.25 and 59.25㎍/mL, respectivity). We measured their inhibitory effects on mushroom tyrosinase and melanin synthesis in B16F1. Both citrus blossom and oilseed rape honey were showed inhibitory activity higher than positive control, arbutin against mushroom tyrosinase. Citrus blossom and oilseed rape honey significantly prevented melanin production without effects on cell viability. When 25㎍/mL of citrus blossom and oilseed rape honey were applied, they reduced melanin contents of B16F1 melanoma cells about 62.25% and 70.25%, respectively. Therefore, this study suggests that citrus blossom and oilseed rape honey may be useful as a natural skin whitening agent.