화학기상증착법을 통해 합성된 그래핀 및 MoSe2를 이용한 반데르발스 수직이종접합 전계효과 트랜지스터

최선연,고은비,권성균,김민희,김설아,이가은,최민철,김현호 한국접착및계면학회 2023 접착 및 계면 Vol.24 No.3

본 van der Waals heterostructure 기반의 트랜지스터의 경우 표면에 불포화 결함(dangling bond) 없이 van der Waals 힘으로만 결합되어 있어 우수한 전자특성을 보이기 때문에 최근 많이 연구되고있다. 하지만, 트랜지스터에 사용되는 이차원 물질들은 대부분 스카치 테이프(mechanical exfoliation) 방법을 기반으로 하는 기초 연구에 머물러 있다. 그렇기 때문에 이를 발전시키기 위해 반데르발스 수직이종접합전계효과 트랜지스터를 제작하는 데 사용되는 모든 소재를 CVD (chemical vapor deposition)에서 성장된소재를 사용하였다. 전극으로는 CVD로 성장된 그래핀을 포토리소그래피 공정을 통해 패터닝하여 사용하였으며, CVD로 성장된 MoSe2를 픽업/전사하는 방식으로, 둘 사이의 반데르발스 이종접합 전계효과 트랜지스터를제작하였다. 본 연구에서는 이를 통해 제작된 소자의 특성을 보았으며 MoSe2의 결함 유무에 따라 트랜지스터의특성에 변화가 있음을 확인하였다.

산화 그래핀을 이용한 물리적 복제 방지 기능(PUF)소자 응용

최선연,윤주웅,이동현,이수빈,조준영,한중탁,유호천,김현호 한국고분자학회 2022 한국고분자학회 학술대회 연구논문 초록집 Vol.47 No.2

Unpredictable Multiple Key Generation Using Graphene Oxide in Physical Unclonable Functions

최선연,이수빈,이동현,조준영,한중탁,장병철,유호천,김현호 한국고분자학회 2023 한국고분자학회 학술대회 연구논문 초록집 Vol.48 No.1

약물상호작용을 중심으로 한 스타틴계 약물의 약물 사용 평가

최선,황유경,이수경,박은하,한옥연,나현오 한국병원약사회 2013 병원약사회지 Vol.30 No.2

A retrospective, cross-sectional study was conducted to evaluate the actual incidence of statin-related adverse events due to drug-drug interaction (DDI) using the Clinical Decision Supporting System (CDSS) with a DDI alert module. Electronic medical records (EMR) were used to estimate the statin-related harmful DDI. We reviewed the full EMR of those patients (1) who were prescribed with statins from 01 January 2010 to 30 June 2011 and (2) whose prescription had a DDI alert message due to contraindicated concomitant drug use. Among 45,080 statin users, 221 (0.49%) patients had DDI alert messages related to their statins. The most frequently DDI alerted statin was rosuvastatin (189/221, 85.5%), and cyclosporine was the most frequently alerted concomitant medication (190/221, 86.0%). Based on the EMR review, 36 of 221 (16.3%) DDI alerted patients had DDI intervention by increasing the dosing intervals of statin (77.8%, 28/36). Most DDI alerted patients had normal laboratory values in serum creatine kinase (CK) and in the liver function test. For the descriptive EMR records review, 3 patients had muscle symptoms sus-pected to be related to statin DDIs. According to the results, contraindicated drug combinations related to statins and actual statinrelated myopathy were rare. However, most patients prescribed with statins could have multiple comorbid conditions; hence, health care professionals should consider different pharmacokinetic profiles of statins and different spectrum of drug interactions in order to select the optimal statin for the patient. Counseling the patients with regards to the risk and warning signs of myopathy and the possibility of drug interactions is also essential.

Stability of Carboplatin in normal saline : dependence on initial dilution concentration and storage time

최선,한옥연,김경진,김순주,황보신이 한국병원약사회 2006 병원약사회지 Vol.23 No.3

Abstract Purpose: Opinions for stability of carboplatin in chloride containing solutions is controversial. This study was conducted to evaluate the stability of carboplatin in normal saline that was representative chloride containing solutions used for dilution of chemotherapeutic agents. Whether initial dilution concentration and storage time were effect factors on carboplatin decreasing rate were also assessed. Methods Dilutions of carboplatin 0.3, 0.5, and 1.0 mg /ml were prepared in normal saline. Aliquots of each solution were transferred to glass containers for storage at room temperature for 24hours. Samples of each solution were analyzed for carboplatin concentration decreasing rate initially and at 10, 12, 24 hours after preparation by high performance liquid chromatography. We compared the decreasing rate of each samples according to initial dilution concentration and storage time. For all these measures, multiple comparisons between samples were made with repeated measured ANOVA. Results Carboplatin was compatible with normal saline. During experimental period carboplatin decreasing rate was under 5% and this value was accepted as stable by Korean regulatory standard. When carboplatin decreasing rate of each samples were compared within same initial dilution concentration, the value increased proportion to storage time (p=0.0001). Compared decreasing rate at same sampling time, the more carboplatin was diluted, the more main ingredients decreased (P=0.0001). Conclusions Carboplatin was compatible with normal saline at room temperature for 24 hours. The more carboplatin was diluted and the longer left in room temperature, the more decreasing rate increased. Initial dilution concentration and storage time were effect factors on carboplatin stability after dilution.

임상시험 약국에 대한 인식도 및 만족도 조사

최선,임영미,한옥연,나현오 한국병원약사회 2012 병원약사회지 Vol.29 No.4

This survey was conducted to examine the internal and external customers’perceptions of and satisfaction in a clinical trial pharmacy in an educational general hospital. The survey questionnaire was sent to 51 internal customers (PI; principal investigator and CRC; clinical research coordinator) and 74 external customers (CRA; clinical research associate). The level of customer satisfaction was measured using a 5-point Likert scale [from 1 (the lowest score) to 5 (the highest score)] for 1) facilities, 2) operating system, and 3) service. Responses were received from 63.2% of CRC, 51.3% of CRA, and 28% of PI, respectively. Overall, the internal customer’s mean satisfaction score was relatively lower than that of the external customer: (1) facilities; 3.50 vs. 4.02 (p=0.0007), (2) operating system; 3.50 vs. 3.91 (p=0.0050), and (3) services; 3.90 vs. 4.14 (p=0.1667). The most highly rated items by both customers were the standard operating procedure (3.90 vs. 4.08, respectively) and attitude of the pharmacy staff (4.19 vs. 4.32, respectively). The lowest rated items by both customers were monitoring room in facilities (3.24 vs 3.63, respectively) and provision of information in the service (3.67 vs 3.84, respectively). According to the results, there is room for improvement in terms of the trial pharmacy facilities and more active communication with customers. Furthermore, internal customers need more specific consultation service for investigator-initiated trials. This result reflects customers’enlarged expectations. Accordingly, a clinical trial pharmacy must make efforts to expand its role and improve its systems, as well as to consistently check customers’needs and satisfaction in its system.

임상시험 재 참여에 대한 임상시험 피험자의 이해도 및 만족도의 영향

최선,최유리,류병은,한옥연,나현오 한국병원약사회 2011 병원약사회지 Vol.28 No.4

This research was conducted to identify the influence of trial participants’general knowledge and satisfaction in a clinical trial on re-participation. From September 2008 to October 2008, a survey questionnaire was distributed to 108 new clinical trial participants in ongoing clinical trials at the investigated Hospital. The participants’general knowledge and satisfaction were measured using a 5-point Likert scale (from 0 to 4) for general attitude towards clinical trials, information source and motive for clinical trial participation,general knowledge of clinical trials, and satisfaction towards one’s own clinical trial. The response rate was 83.3% and 63.3% of respondents agreed with re-participation. We grouped respondents as ‘re-participation acceptor’and ‘re-participation decliner’based on respondents’intent of re-participation. Most re-participation acceptors had more positive attitude towards clinical trial (p<0.05). Similarly, more re-participation acceptors evaluated their general knowledge level positively, especially for‘ background and objective’,‘ frequency of visit and test’and investigational drug dosing’(p<0.05). Among the investigated items, “interest in trials in general”was the most important factor, while “needs for clinical trial into some area”,and“ subjective satisfaction level”were significant effect factors (p<0.05). According to the results, clinical trial staff should make an effort to interest possible participants by providing proper information, and help them recognize the need for clinical trials and the benefits of clinical trials through consistent communication.

피부 섬유아세포에서 진달래 추출물의 항산화 및 항노화 효능

최선일 ( Choi S. I. ),이사라 ( S. Lee ),이혜진 ( H. J. Lee ),김병직 ( B. J. Kim ),여주홍 ( J. Yeo ),정태동 ( T. D. Jung ),조봉연 ( B. Y. Cho ),최승현 ( S. H. Choi ),한웅호 ( X. Han ),심완섭 ( W. S. Sim ),이진하 ( J. H. Lee ),이옥환 ( O. 강원대학교 농업생명과학연구원(구 농업과학연구소) 2017 강원 농업생명환경연구 Vol.29 No.3

현재 식품 공전에 식품원료로 사용이 가능한 것으로 등록된 국내 산림지역 자생 식물을 식품산업에 활용하고자 국내 자생식물 45종을 선별하였고, 본 연구팀의 선행연구에서 45종의 항산화 활성을 평가한 결과, 다른 종들과 비교하여 우수한 항산화 활성을 보인 진달래를 본 연구에서 사용하였다. 피부 섬유아세포에 Hydrogen peroxide로 산화적 스트레스를 유도한 상태에서 진달래 추출물의 세포 보호효과 및 세포내 항산화 효과를 관찰하였다. 세포 보호효과를 측정한 결과 Hydrogen peroxide로 인해 세포 생존율이 감소한 반면 진달래 추출물처리로 세포의 산화적 손상이 억제되는 것으로 관찰되었다. H2-DCFDA 염색을 통해 세포내 항산화 효과를 확인한 결과 진달래 추출물에 의한 활성산소 저감을 확인하였다. 또한 SA-β-galactosidase assay를 통해 항노화 효과를 확인한 결과 진달래 추출물을 처리했을 때 세포의 SA-β-galactosidase 활이 줄어드는 것을 확인할 수 있었다. 이상의 결과를 종합하여 볼 때 진달래 추출이 Hydrogen peroxide로 산화적 스트레스를 유도한 상태에서 세포 생존율을 증가시키고 세포내 활성산소의 생성을 억제하며 이로 인해 세포 노화억제가 관찰되어 천연물에서 유래한 기능성 식품원료로서의 활용도가 매우 넓을 것으로 판단된다. Skin aging is associated with increased reactive oxygen species (ROS) production by human cells, which is characterized by wrinkles and atypical pigmentation. Use of antioxidants is an effective approach to prevent symptoms associated with ROS-induced skin aging. Although synthetic antioxidants have been used to remove free radicals, several studies have reported that the synthetic antioxidants have side effects that cause long-term edema, which subsequently results in cancer and intracellular toxicity. Therefore, the study of safe and functional antioxidants derived from natural products is necessary for replacement of synthetic antioxidants. In this study, the antioxidant and anti-aging effect of Rhododendron mucronulatum Turcz. extracts (RTE) on human dermal fibroblasts (HDFs) were investigated. We confirmed that RTE markedly reduced hydrogen peroxide-induced cell damage, intracellular ROS, and oxidative stress-induced senescence in HDFs. These results suggest that RTE is potential natural source of antioxidant and anti-aging compounds.

피부 섬유아세포에서 갈참나무 잎 추출물의 항산화 및 항노화 효능

최선일,이종석,이사라,여주홍,정태동,조봉연,최승현,심완섭,한웅호,이진하,김종대,이옥환,Choi, Sun-Il,Lee, Jong Seok,Lee, Sarah,Yeo, Joohong,Jung, Tae-Dong,Cho, Bong-Yeon,Choi, Seung-Hyun,Sim, Wan-Sup,Han, Xionggao,Lee, Jin-Ha,Kim, Jong Dai 한국식품위생안전성학회 2018 한국식품위생안전성학회지 Vol.33 No.2

본 연구는 예부터 약용으로 사용되었거나, 현재 식품공전에 식품원료로 사용이 가능한 것으로 등록된 국내 산림지역에 자생하는 식물을 식품산업에 활용하고자 선행연구에서 우수한 항산화 활성을 보인 갈참나무 잎을 본 연구에서 사용하였다. 70% 에탄올을 이용하여 추출한 갈참나무 잎을 이용하여, hydrogen peroxide로 산화적 스트레스를 유도한 피부 섬유아세포에서의 세포 보호효과, 세포내 항산화 효과 및 항노화 효과를 측정하였다. 세포 독성을 평가한 결과 25, 50 및 $100{\mu}g/mL$의 갈참나무 잎 추출물을 처리하였을 때 모두 독성을 나타내지 않았으며, hydrogen peroxide로 산화적 스트레스를 유도한 상태에서는 세포를 보호하여 농도 유의적으로 세포생존율이 증가하였다. 특히, $100{\mu}g/mL$의 농도에서는 양성대조군으로 사용한 $50{\mu}M$ ascorbic acid 수준까지 세포 생존율이 증가하였다. 세포내 항산화 효과를 확인 하기위해 사용한 $H_2-DCFDA$ assay에서는 형광현미경과 형광흡광도 측정에서 모두 농도 유의적으로 세포내 ROS 저감 활성을 확인하였고 갈참나무 잎 추출물을 $100{\mu}g/mL$ 농도로 처리했을 때는 $50{\mu}M$ ascorbic acid와 비슷한 세포내 항산화 효과를 나타내었다. 또한 SA-${\beta}$-galactosidase assay를 이용한 갈참나무 잎 추출물의 피부 섬유아세포에 대한 항노화활성은 ROS 생성 억제 효과와 유사한 경향으로 갈참나무 잎 추출물의 농도 유의적으로 세포 노화 억제효과를 확인하였다. 이상의 결과를 종합하여 볼 때 갈참나무 잎 추출물이 hydrogen peroxide로 인한 산화적 스트레스 상태에서 세포 보호효과, 항산화 효과 및 항노화 효과가 관찰되어 기능성 식품원료로서의 활용도가 매우 넓을 것으로 판단된다. The skin of the human body occupies the largest surface area of the body and acts as a protection for the person's internal organs. As such, the skin is a major target of oxidative stressors, and these oxidative stressors are known to contribute to skin aging over the course of time. For the most part, an antioxidant is an effective approach to utilize to prevent symptoms related to the reactive oxygen species (ROS)-induced aging of the skin. Therefore, we investigated the antioxidant and anti-aging activity of the leaves of the Quercusaliena Blume extract (QBE). In our study, we confirmed that the cell viability tested with XTT {2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide innersalt} assay was not affected up to a concentration of $100{\mu}g/mL$. In addition, the cell viability of HDF cells induced by hydrogen peroxide was recovered from 81% to 104% after treatment with QBE, which showed the greater protective effect than that of ascorbic acid. Treatments of QBE dose-dependently inhibited reactive oxygen species (ROS) production in HDF cells induced by hydrogen peroxide, which correlated with their protective effects on cell viability. Since QBE treatment exhibited the suppression effect of skin aging by decreasing the ROS production, QBE could be used as a not only natural anti-aging but also antioxidant resource.

공장 및 수공업 생산 찐빵의 품질특성

조봉연 ( B. Y. Cho ),최승현 ( S. H. Choi ),김태선 ( T. S. Kim ),박영은 ( Y. E. Park ),김재민 ( J. M. Kim ),최선일 ( S. I. Choi ),정태동 ( T. D. Jung ),오지원 ( J. W. Oh ),이진하 ( J. H. Lee ),홍종민 ( J. M. Hong ),이옥환 ( O. H. Lee 강원대학교 농업생명과학연구원(구 농업과학연구소) 2016 강원 농업생명환경연구 Vol.28 No.1

본 연구에서는 현대식 대량제조 방식의 공장 생산 찐빵과 소규모 업체에서의 손찐빵의 품질특성을 평가하고자 texture analyzer를 이용한 조직감, 주사 전자현미경 관찰 및 관능검사를 실시하였다. 분석에 사용된 시료는 팥찐빵, 단호박찐빵, 흑미찐빵으로 각각 공장 생산 찐빵과 수공업 생산 찐빵으로 구분하여 사용하였다. 점착성과 응집성에서 수공업 생산 방식으로 제조한 찐빵이 공장 생산 방식으로 제조한 찐빵에 비해 높은 값을 나타내었다. 경도에서는 공장 생산 방식으로 제조한 찐빵이 수공업 생산 방식으로 제조한 찐빵에 비해 높은 값을 나타내었다. 주사 전자현미경 관찰 결과에서는 수공업 생산 방식으로 제조한 찐빵이 공장 생산 방식으로 제조한 찐빵보다 기공의 크기가 큰 것으로 나타났다. 관능검사에서는 풍미(먹음직스러움) 항목과, 전반적인 기호도 항목에서 수공업 생산 방식으로 제조한 찐빵이 공장 생산 방식으로 제조한 찐빵보다 높은 점수를 나타내었다. 본 연구의 결과는 향후 찐빵 제품의 품질 향상과 개발에 필요한 기초자료로 활용될 것으로 사료된다. This study was to investigate the quality characteristics of factory-style and handmade-style steamed bread products using texture analyzer, scanning electron microscope (SEM) and organoleptic evaluation. Our results showed that the handmade-style steamed bread were higher in adhesiveness and cohesiveness than those of factory-style steamed bread but hardness was lower. According to the SEM, many equally distributed large holes were present on the handmade-style steamed bread surface. In the organoleptic characteristics, flavor (taste) and overall experience of handmade-style steamed bread evaluated higher than those of factory-style steamed bread. This study was to provide basic data on quality characteristics of steamed bread that will be useful for the improving quality and developing steamed bread.