Clinical Impact of Somatic Variants in Homologous Recombination Repair-Related Genes in Ovarian High-Grade Serous Carcinoma

최민철,황소현,김세화,정상근,박현,주원덕,송승훈,이찬,김태헌,강혜윤,안희정 대한암학회 2020 Cancer Research and Treatment Vol.52 No.2

Purpose In this study, we investigated the frequencies of mutations in DNA damage repair genes including BRCA1, BRCA2, homologous recombination genes and TP53 gene in ovarian highgrade serous carcinoma, alongside those of germline and somatic BRCA mutations, with the aim of improving the identification of patients suitable for treatment with poly(ADPribose) polymerase inhibitors. Materials and Methods Tissue samples from 77 Korean patients with ovarian high-grade serous carcinoma were subjected to next-generation sequencing. Pathogenic alterations of 38 DNA damage repair genes and TP53 gene and their relationships with patient survival were examined. Additionally, we analyzed BRCA germline variants in blood samples from 47 of the patients for comparison. Results BRCA1, BRCA2, and TP53 mutations were detected in 28.6%, 5.2%, and 80.5% of the 77 patients, respectively. Alterations in RAD50, ATR, MSH6, MSH2, and FANCA were also identified. At least one mutation in a DNA damage repair gene was detected in 40.3% of patients (31/77). Germline and somatic BRCA mutations were found in 20 of 47 patients (42.6%), and four patients had only somatic mutations without germline mutations (8.5%, 4/47). Patients with DNA damage repair gene alterations with or without TP53mutation, exhibited better disease-free survival than those with TP53 mutation alone. Conclusion DNA damage repair genes were mutated in 40.3% of patients with high-grade serous carcinoma, with somatic BRCAmutations in the absence of germline mutation in 8.5%. Somatic variant examination, along with germline testing of DNA damage repair genes, has potential to detect additional candidates for PARP inhibitor treatment.

체형에 따른 골프 어드레스 동작의 주요 관절각도 비교

최민철,이창진,임영태 한국운동역학회 2011 한국운동역학회지 Vol.20 No.4

This study was aimed at comparing selected joint angles at the golf address stance by categorizing three different body types. 43elite male golfers were selected and 9 of them turned out to be the ectoderm while 24 of them to be the mesoderm. The remaining 10 subjects were the endoderm. The measurement was carried out at the address stance with the number 7 iron and the driver. The result showed that the angle of trunk flexion did not different among body types. The trunk tilting angle became more inclined to the right side, which confirmed the guidelines from most of golf lesson books, for bigger people since they tended to put more weight on the right foot. The angle of both knees showed similar but the right knee was bent more than the left knee. The target direction and body alignment faced more to the left side than the target spot because of the influence of open stance and natural aiming position. It seems that pelvis and knees turned a little bit more to the right side than the target direction in order to maintain the parallel. Overall, significant differences among body types were found at the trunk tilting angle and pelvis-target alignment and golf address configuration can be differentiate by these factors.

Aloe vera투여가 Cobalt-60 감마선 조사를 받은 마우스의 생존율과 조혈간세포에 미치는 영향

최민철,성재기,Choi,Min-cheol,Sung,Jai-Ki 한국임상수의학회 1990 한국임상수의학회지 Vol.7 No.2

The present study was carried out to investigate whether the aloe had a radioprotective effect in mice exposed to cobalt-60 gamma radiation or not. The survival ratio of mice for 30 days, hematopoiesis of blood-forming stem cells by spleen colony assay, chromosomal aberration frequency of bone marrow cells and histopathological findings of bone marrow were investigated. The survival ratios of aloe administered groups with concentration of 250, 500, 1,000 and 1,500mg for 3 days before irradiation and control group in cobalt-60 gamma irradiated mice(700rads whole body irradiation, dose rate of 50rads/min.) were 77.4, 79.3, 80.6, 90.0 and 53.1%, respectively. The survival ratios of pre-irradiation aloe administered groups were superior to those of post-irradiation aloe groups and control group. In spleen colony assay, Aloe vera administration before irradiation enhanced the recoveries of numbers of blood-forming stem cells of bone marrow of irradiated mice. There were decreased chromosomal aberrations of bone marrow cells at the first day after irradiation in aloe administered groups compared to that of control group. Histopathological findings in the bone marrow of irradiated mice were hypocellularity due to the depletion of myelocytes, abundant of fat vacuoles and these changes were weakened in aloe administered groups compared to that of control group.

Aloe vera가 Cobalt-60 gamma선 조사를 받은 쥐의 혈액성분에 미치는 영향

최민철,Choi,Min-chul 대한수의사회 1990 대한수의사회지 Vol.26 No.1

The effect of Aloe vera administration(500mg/kg, P.O.) for 21 days on the total white blood cell, packed cell volume, hemoglobin, total protein, serum glutamic pyruvic transaminase and sorbitol dehydrogenase values in cobalt-60 gamma irradiated rats was s

티타늄 판상에 배양된 정상인 조골세포에 대한 효과에 관한 연구

최민철,오충훈,천재식 대한구강악안면병리학회 2008 대한구강악안면병리학회지 Vol.32 No.1

Low energy photon irradiation by light in the far red to near infrared spectral range(630~1000nm) using low energy lasers or light emitting diode arrays has been found to modulate various biological processes in cell culture and animal models. The purpose of this study was to examine the light emitting diode irradiation effect on activity of normal human osteoblast on titanium plate in vitro by various energy density, and to observe morphologic change of NHost on titanium plate and to analysis concentration of Ca++, IP and ALP. NHost were cultured in DMEM containing 10% FBS, and observed by inverted microscope for attatchment to the surface of titanium plate. Ca++, I.P., and alkaline phosphatase(ALP) concentration in medium was calculated during 4 weeks, which was treated with Wilcoxon rank, Anova test and linear regression. Morphologic changes showed LED produced in vitro increases of cell growth of 144~256% in NHost. During a culture period, Ca++ concentration was decreased. LED treatment(>3J/cm2) stimulate calcium consumption in NHost. Statistically, a significant difference was not found between LED power density. LED treated group(>3J/cm2) had higher total inorganic phosphate concentrations than control group in NHost. Statistically, a significant difference was not found between LED power density. No significant changes were observed between ALP acitivity and LED treatment. In spite of LED power density, there were rapid growth rate of NHost and no significant of Ca++, IP and P concentration but these concentration showed predominant change than that of control.

Prevalence of germline BRCA mutations among women with carcinoma of the peritoneum or fallopian tube

최민철,배진식,정상근,박현,주원덕,송승훈,이찬,김지호,이기찬,이성훈,이제호 대한부인종양학회 2018 Journal of Gynecologic Oncology Vol.29 No.4

OBJECTIVE: The aim of the present study was to assess the frequency of germline mutations in patients with peritoneal carcinoma (PC) or the fallopian tube carcinoma (FTC), using a multi-gene panel. METHODS: Twenty-six patients diagnosed with either PC or FTC between January 2013 and December 2016 were recruited consecutively. Germline DNA was sequenced using a 6-gene next generation sequencing (NGS) panel following genetic counseling. Surgico-medical information was obtained from hospital records. Genetic variations were detected using the panel and were cross-validated by Sanger direct sequencing. RESULTS: Germline BRCA1/2 mutations were identified in 6 patients (23.1%). Four were detected in patients with PC and 2 were in FTC patients. No mutations were detected in TP53, PTEN, CDH1, or PALB2. We identified 11 variant of uncertain significance (VUS) in 9 patients; 2 in BRCA1, 3 in BRCA2, 2 in TP53, and 4 in CDH1. We also detected a CDH1 c.2164+16->A VUS in 3 patients. CONCLUSION: The prevalence of germline BRCA1/2 mutations in patients with PC or FTC is comparable to that of BRCA1/2 mutations in epithelial ovarian cancer patients.

초음파유도 난포란 채취를 위한 기본 기술의 개발 2 : 소의 마취방법과 채란기구의 개발

최민철,조성근,강태영,박준규,손우진,이효종 韓國受精卵移植學會 1997 한국동물생명공학회지 Vol.12 No.2

This experiment was carried out to develop the anesthetic methods for ultrasonography and a new simplified disposable needle guidance device for ovum pick-up(OPU) in cows. Three different anesthetic methods were applied as. 1) epidural analgesia only with 2% lidocaine(20~30 ml), 2) epidural analgesia with 2% under general sedation with xylazine, 3) epidural analgesia with 2% lidocaine under general sedation with detomidine. We evaluated the anesthetic effects with items such as relaxation of anal sphincter, tail movement and rectal wall, retractability of both ovaries, additional anesthesia and possibility of OPU. Through this experiment, the above three anesthetic methods were applicable to OPU, but the epidural anlagesia under general sedation with detomldine was most effective for OPU. We developed a new disposable needle guidance device with stainless steel tube. With this, disposable needles can be easily attatchable to any other intravaginal probes. And also, it was found to he practical, economic and effective for OPU with the recovery rate of 51.2%.

AHP 분석의 문제점과 수정가중치모형의 개발

최민철 대한경영정보학회 2020 경영과 정보연구 Vol.39 No.2

This study examines problems with using the conventional analytic hierarchy process (AHP) method and proposes a method of weight adjustment as a modification of AHP. AHP is a method for transforming complex decision problems into a hierarchal structure, which is composed of elements in the upper and lower levels and then using pairwise comparisons to evaluate these elements and subsequently to obtain their relative weights. The elements' relative importance is reliable if the elements in the lower hierarchical levels (sub factors) that comprise each element in the upper hierarchical level (primary factor) are equal in number. In other words, if the number of sub factors is different for each primary factor, a serious error is expected as a result. Therefore, this study proposes a modification of AHP that can avoid such an error when AHP is used. Specifically, an error that arises from different number of sub factors (matrix size) can be overcome by making the number of sub factors identical for each primary factor. The resulting model has been validated through the applications in different AHP hierarchical structures. 본 연구는 AHP 적용상의 문제점을 살펴보고 올바른 적용방법을 위한 ‘AHP 수정가중치모형'을 개발하는 연구이다. 여기서 AHP는 복잡한 의사결정 문제를 계층화 하여 상위수준요인과 하위수준요인들로 분해하고, 그 요인들에 대한쌍대비교를 통해서 요인들에 대한 상대적인 가중치를 도출하는 방법이다. 하지만 AHP 계층구조에서 각 상위수준요인(대항목)을 구성하는 하위수준요인(소항목)들의 개수가 같을 경우에는최종가중치가 바르게 나타나지만 각 대항목을 구성하는 소항목들의 개수가 다른 경우에는 심각한 오류가 발생한다. 이에 본 연구에서는 이를 개선할 수 있는 ‘AHP 수정가중치모형'을 개발하였다. 구체적으로 AHP 분석의 오류가 소항목의 개수, 즉 규모가 다를 때 발생하는 것에 착안하여 소항목 개수의 규모를 상대적으로 같게 만들어서 그 오류를제거하였다. 그리고 개발된 모형을 다양한 AHP 계층구조에 적용하여 검증하였다. 구체적으로 첫 번째 모형검증에서는 4개의 대항목과 각각 개수가 다른 소항목으로 구성되어 있으며 대항목과 소항목의 중요도가 같다고 가정하였고, 두 번째 모형검증에서는 2개의 대항목과 각각 개수가 다른 소항목으로 구성되어 있으며 모든 대항목과 소항목의 중요도가 다르다고 가정하였다. 그리고 세 번째 모형검증에서는 실제 선행연구에 적용하였다. 그 결과 본 연구에서 개발된 ‘AHP 수정가중치모형'이 AHP 분석의 문제점을 효과적으로 해결할 수 있었다.

유전성 부인암의 예방, 조기검진 및 치료

최민철 대한산부인과학회 2017 대한산부인과학회 학술대회 Vol.103 No.-

Osteochondrodysplasia in three Scottish Fold cats

최민철,장진화,Joohyun,Jung,Sunkyoung,Oh,Sungok,Lee,Gyeongmin,Kim,Haksang,Kim,권오경,윤정희 대한수의학회 2007 JOURNAL OF VETERINARY SCIENCE Vol.8 No.3

'스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

This report explains typical radiographic features of Scottish Fold osteochondrodysplasia. Three Scottish Fold cats suffering from lameness were referred to the Veterinary Medical Teaching Hospital, Seoul National University, Korea. Based on the breed predisposition, history, clinical signs, physical examination, and radiographic findings, Scottish Fold osteochondrodysplasia was confirmed in three cases. Radiographic changes mainly included exostosis and secondary arthritis around affected joint lesions, and defective conformation in the phalanges and caudal vertebrae. The oral chondroprotective agents such as glucosamine and chondroitin sulfate make the patients alleviate their pain without adverse effects.