Critical Role of the Cysteine 323 Residue in the Catalytic Activity of Human Glutamate Dehydrogenase Isozymes

조성우,Seung-Ju Yang,Eun Hee Cho,Myung-Min Choi,Hyun-Ju Lee,허재완,최수영 한국분자세포생물학회 2005 Molecules and cells Vol.19 No.1

The role of residue C323 in catalysis by human gluta-mate dehydrogenase isozymes (hGDH1 and hGDH2) was examined by substituting Arg, Gly, Leu, Met, or Tyr at C323 by cassette mutagenesis using synthetic human GDH isozyme genes. As a result, the Km of the enzyme for NADH and -ketoglutarate increased up to 1.6-fold and 1.1-fold, respectively. It seems likely that C323 is not responsible for substrate-binding or coenzyme-binding. The efficiency (kcat/Km) of the mu-tant enzymes was only 1114% of that of the wild-type isozymes, mainly due to a decrease in kcat values. There was a linear relationship between incorporation of [14C]p-chloromercuribenzoic acid and loss of enzyme activity that extrapolated to a stoichiometry of one mol of [14C] incorporated per mol of monomer for wild type hGDHs. No incorporation of [14C]p-chloromer-curibenzoic acid was observed with the C323 mutants. ADP and GTP had no effect on the binding of p-chloromercuribenzoic acid, suggesting that C323 is not directly involved in allosteric regulation. There were no differences between the two hGDH isozymes in sen-sitivities to mutagenesis at C323. Our results suggest that C323 plays an important role in catalysis by hu-man GDH isozymes.

광릉긴나무좀, 왕녹나무좀, 노랑애나무좀 성충에 대한 포스핀의 훈증활성

조성우,김성일,김현경,김길하,Cho, Sung Woo,Kim, Sung Il,Kim, Hyun Kyung,Kim, Gil-Hah 한국응용곤충학회 2019 한국응용곤충학회지 Vol.58 No.1

많은 산림해충들은 목재검역에서 문제가 되고 있어 포스핀($PH_3$)을 이용하여 광릉긴나무좀, 왕녹나무좀, 노랑애나무좀의 성충에 대한 살충 활성을 조사하였다. 3종의 나무좀류에 대하여 99% 살충활성을 보이는 LCT값은 각각 3.192 (광릉긴나무좀), 0.994 (왕녹나무좀), $0.501mg{\cdot}h/L$ (노랑애나무좀) 순으로 나타났다. 포스핀을 처리한 3종의 나무좀류는 시간이 지남에 따라 살충활성도 증가하였다. 특히 광릉긴나무좀에서는 0.4 mg/L 이상의 농도에서 약제처리 7일 후에 100%의 사충률을 보였다. 이러한 결과로 볼 때 포스핀이 3종의 목재해충 성충에 대해 메칠브로마이드의 대체훈증제로 이용될 수 있을 것으로 판단된다. Many forest pests have caused problems for wood quarantine. The fumigation activity of phosphine ($PH_3$) was examined for the adults of three wood-boring insect pests. The $LCT_{99}$ values for Platypus koryoensis, Cryphalus fulvus, and Xyleborus mutilates were 3.192, 0.994, and $0.501mg{\cdot}h/L$ at $20^{\circ}C$, respectively. The effectiveness of $PH_3$ was increasingly time dependent for all doses tested in all three species. In particular, P. koryoensis showed 100% mortality at doses higher than 0.4 mg/L 7 days after fumigation. These results indicate that methyl bromide could be substituted for $PH_3$ for adults of these three species of wood pest.

꽃노랑총채벌레와 대만총채벌레에 대한 51종의 살충제 감수성 평가

조성우,경예진,조선란,신소은,정대훈,김성일,박근호,이승주,이영수,김민기,조인준,구현나,김현경,김길하,Cho, Sung Woo,Kyung, Yejin,Cho, Sun-Ran,Shin, Soeun,Jeong, Dae Hun,Kim, Sung Il,Park, Geun-Ho,Lee, Seung-Ju,Lee, Young-Su,Kim, Min-Ki,Jo, In-Jun 한국응용곤충학회 2018 한국응용곤충학회지 Vol.57 No.3

시판되고 있는 51종(단제 21종과 합제 30종)의 살충제를 이용한 꽃노랑총채벌레와 대만총채벌레 성충에 대한 약제 감수성을 검토한 결과 90% 이상의 살충률을 보인 약제 15종을 선발하였으며, 시판되고 있는 합제에서 많은 유효성분들이 오남용되고 있다고 보여진다. 2종의 총채벌레에 대한 살충제 감수성을 비교한 결과 모든 약제에 대하여 대만총채벌레가 꽃노랑총채벌레에 비해 높은 감수성을 보이기 때문에 꽃노랑총채벌레에 효과 있는 약제로 동시방제가 가능할 것으로 판단된다. 꽃노랑총채벌레를 대상으로 선발된 15종의 약제를 이용하여 약효발현속도($LT_{50}$과 $LT_{95}$), 침투이행성, 잔효성을 비교하였다. 약효 발현속도를 $LT_{95}$ (hour)값으로 비교했을 때, chlorpyrifos WP, chlorpyrifos + diflubenzuron WP가 2시간 이내로 가장 빨랐고, spinetoram WG은 62.3시간으로 가장 느렸다. Chlorfenapyr SC에서 뿌리와 엽면을 통한 침투이행 효과가 나타났고, spinetoram WG에서 엽면을 통한 침투이행 효과만이 나타났다. 약제 잔효성 실험에서 chlorfenapyr SC는 14일, benfuracarb WG와 chlorpyrifos WP는 3일까지 효과가 지속되었다. 꽃노랑총채벌레 야외집단(화훼재배지와 과채류재배지)간의 감수성 비교에서는 화훼재배지에서 채집된 집단이 더 낮은 약제 감수성을 보였다. The susceptibility of the western flower thrips, Frankliniella occidentalis and garden thrips, Frankliniella intonsa was evaluated using 51 commercial insecticides. 15 kinds of insecticides which showed more than 90% mortality against both thrips, F. occidentalis and F. intonsa was selected. Many active ingredients were misused and abused in commercial mixture formulation insecticides. Since the F. intonsa was more susceptible than F. occidentalis, it was considered that both thrips can be controlled by insecticides that showed insecticidal activity on the F. occidentalis. Lethal time ($LT_{50}$ and $LT_{95}$), systemic toxicity and residual toxicity of selected insecticides were compared. Both chlorpyrifos WP and chlorpyrifos + diflubenzuron WP revealed the fastest toxicity within 2 h ($LT_{95}$), while spinetoram WG revealed the slowest toxicity as 62.3 h ($LT_{95}$). Chlorfenapyr SC showed toxicity at foliar and drenching application while spinetoram WG was toxic only in foliar application. Chlorfenapyr SC showed residual effect at 3, 5, 7, 10, 15 days after treatment and both benfuracarb WG and chlorpyrifos WP showed residual effect at 3 days after treatment. As a result of treatment of selected insecticides for field population of F. occidentalis, the population collected from horticultural crops showed lower susceptibility than the population collected from vegetable crops.

EnergyPlus를 이용한 수평형의 지열 히트펌프와 어스튜브를 조합한 시스템의 성능 검토

조성우,Cho, Sung-Woo 한국지열에너지학회 2017 한국지열에너지학회논문집 Vol.13 No.2

This study is performed to performance of the combined system the GSHP (Ground Source Heat Pump) system with the Earth tube system using EnergyPlus program. The Earth tube system using fan is characteristics as supply lower (higher) air temperature than outdoor air temperature in cooling and heating seasons, the GSHP system is characteristics as small indoor air temperature variation range. As the results of Earth tube + GSHP system simulation, GSHP power can be reduced than the GSHP single operation as 17.3% in cooling seasons and 32.5% in heating seasons, the GSHP design capacity can be replaced more small size.

척추 측만증의 보존적 치료에 관한 최신 지견 - PubMed 검색을 위주로 2008년 이후 -

조성우,이승연,Cho, Sung Woo,Lee, Seung Yeon 대한한방소아과학회 2013 대한한방소아과학회지 Vol.27 No.1

Objectives Scoliosis is that spine's arrangement was changed to be curved. Mostly scoliosis was found at childhood and recently rate of scoliosis is increasing. Causes of scoliosis were very various, but the patients are due to idiopathic reasons. Scoliosis was treated by conservative treatment or surgery treatment. If scoliosis is diagnosed early enough, it can be treated with conservative treatment. Methods We are searching PubMed by keywords and found 47 theses complied with requirements. Results Studies of conservative treatment of Scoliosis were active. Conservative treatments are considered before surgery treatment and there was a set guideline of conservative treatment. Also, there were several studies about effectiveness of conservative treatment and quality of life, mental stress. Studies about various kinds of conservative treatment were found as well. There were brief studies about introduction of new brace, effectiveness of several braces, and case studies. Conclusions Conservative treatment is very effective choice before surgery treatment at Scoliosis patient.

공동주택에서 마스터룸(Master room) 평면 유형별 수요자 의식 조사 연구

조성우,이수용,문출성,오세규,Cho, Sung-Woo,Lee, Su-Yong,Moon, Cul-Seong,Oh, Se-Gyu 한국주거학회 2008 한국주거학회 논문집 Vol.19 No.6

The residential environment is a place which reflects the gradual evolution of human consciousness because it is a cultural space the term, 'houses' is not relevant in this context as it literally means 'contains'. The long history of human living habits cannot be 'contained' within a space, but can be 'expressed' by a space and by the way the space is used. Furthermore, due to the way it is typically constructed, the basic characteristics of the house usually cannot be rapidly changed to accommodate new habits. However, recent changes that have taken place to the characteristics of the traditional master room suggest that traditional societal values associated with patriarchy have diminished. The current trend where priority is given to children's education also affects such a change. Existing researches related to the master room is confined to the behavioral use of the space and the composition of the physical space, while research on the master room its plane type has not been carried out. Therefore, in this study, apartment model houses are selected, and are classified according to the types of master room. An analysis is then carried out of the master room based on the occupants' requirements in terms of consciousness. Any planning modifications where required are then suggested. This research comprises the followings stages. First, an examination is carried out of existing research on the master room in order to understand its background and spatial components. A definitiones of related terms is also provided. Second, an analysis is carried out of the model houses exhibited at Suwan District, examining the typical standard that has been selected for the master room. Third, an analysis is conducted of the topics and questions to be selected for a questionnaire. Fourth, an analysis is carried out of the consciousness, satisfaction and requirements of the residents, as respondents in the questionnaire, related to the master room. and Research is then conducted of the architectural methods for planning the master room. Finally, future countermeasures for planning the master room are described based on the above analysis and research findings.

사람 혈소판에서 분리한 Transforming Growth Factor - β가 초회 배양된 Mesangial 세포의 복제 및 Collagen 합성에 미치는 영향

조성우,박수길,김금이,이희봉,이재담 ( Sung Woo Cho,Su Kil Park,Geum Yi Kim,Hee Bong Lee,Jae Dam Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.3

The effect of TGF-β on cell replication and collagen synthesis in primarily cultured mesangial cells from rats was studied in vitro. The TGF-β was purified from human platelets by acidic ethanol extraction and gel filtration with FPLC. The effect of TGF-β on the DNA synthesis and the collagen synthesis in mesangial cells depended on both TGF-β concentration and cell density in monolayer culture. Concentrations of TGF-β ranging from 0.1∼15 ng/ml were evaluated for their effects upon DNA synthesis. In confluent cells, when compared to the growth rate in standard medium, TGF-β concentrations in the range 0.1∼15 ng/ml reduced a cell proliferation up to 35%, suggesting inhibition of cell division. The effect of TGF-β on collagen synthesis in mesangial cells was biphasic. In confluent cells, the addition of TGF-β induced a 2-fold increase in the production of collagen when compared with cells grown in standard medium. The maximal effect of TGF-β occurred at 0.5 ng/ml. At higher concentrations there was a decrease in the action of TGF-β with the effects on collagen production. In subconfluent cells, however, TGF-β showed no significant effects on the DNA synthesis and the collagen synthesis at all concentrations. These results support that TGF-β may play an important role as a multifunctional regulator in the pathogenesis of glomerulosclerosis. v

전통주거건축 공간구성에 따른 안방창호 구성에 관한 연구 - 전라남도 지역 중상류 주택을 중심으로 -

조성우,이재홍,문출성,천득염,Cho, Sung-Woo,Lee, Jae-Hong,Moon, Chul-Seong,Cheon, Deuk-Youm 한국주거학회 2014 한국주거학회 논문집 Vol.25 No.3

Korean traditional house has inherented construction characteristics that contain human requirements with the adaptation to nature's order. As for traditional buildings, there are a lot of facilities like palaces, Buddhist temples, Confucianism architectures, government facilities, etc. Among them, housing construction is not only closely connected to our lives, but also plays an important role to connect our lives from the past to present. The master room in a traditional korean house is responsible for playing a center role of the residential life which implies the traditional values responsible for multi-functions and it is also the sphere of the living space. Therefore, this thesis considers the residential environmental adjustment method and the behaviors centered around the main buildings of Jeonnam Province. And also investigates the main reason for windows and doors formation. From this, we would like to further evaluate the master room life, and the inner space constructions following the spacial and organizational analysis of windows and doors. We would like to analyze the space organization methods, environmental adjustment methods and the usages of traditional buildings. Using the modern interpretation as basis, we would like to use the foundation materials to reflect single family housing plans.

밀 유전 배경에서 호밀 B 염색체가 감수분열 염색체 접합에 미치는 영향

조성우,Cho, Seong-Woo 한국자원식물학회 2022 한국자원식물학회지 Vol.35 No.5

The effect of rye B chromosome (rye B) on chromosome association was investigated in meiosis of wheat addition line. The wheat addition line was with one Leymus mollis chromosome and one L. racemosus chromosome which are under homoeologous relationship. Chromosome behavior of the two Leymus chromosomes in wheat genetic background was revealed by genomic in situ hybridization. In the first metaphase, most of the two Leymus chromosomes showed univalent in the wheat addition line without rye B (98.1 ± 0.5%). On the other hand, the wheat addition line with rye B showed higher frequency of bivalent (10.3 ± 0.2%) than wheat addition line without rye B (1.9 ± 0.5%). The wheat addition line without rye B showed abnormal bivalents with abnormal structure while the wheat addition line with rye B showed normal bivalent in low frequency. By rye B, some bivalent was composed of wheat and L. racemosus, and some trivalent was composed of wheat bivalents with L. mollis chromosome. Also, some wheat bivalents showed hyper-crossover, so those wheat bivalents showed abnormal structure compared to other wheat bivalents with normal structure such as ring, rod, and pan.

참여디자인을 통한 세대(世代)별 공동주택 평면계획 선호특성에 관한 연구

조성우,오세규,유포숙,Cho, Sung-Woo,Oh, Se-Gyu,Yu, Po-Sook 한국주거학회 2014 한국주거학회 논문집 Vol.25 No.5

In the upcoming 21st century, we predict change and development in various aspects. Some fundamental causes of change in housing lifestyles such as the change in family make-up are gradually becoming more and more realistic problems as the society is shifting to the younger generation family make up refer to the rapid increase of the agedpopulation, growth of single households, the drop in the number of family members, the change in the economic situation and technology level, and change in values. The purpose of this research is to, more away from the standardized supply of housing and to approach apartment housing plans in multilateral aspects, compare and analyze the difference between the housing demands of different generations and the influence that the current housing type has on demand forecasting by case studies of Unit Living Space planning that we will utilize the participating design method in which parents and children took part in.