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Differential expression of Chinese cabbage infected by Myzus persicae and Plutella xylostella
구현나,조선란,문일성,김길하 한국응용곤충학회 2013 Journal of Asia-Pacific Entomology Vol.16 No.1
Plants can exhibit marked differences in gene expression due to herbivore attack. In this study, we compared global proteome profiles and the expression pattern of defense-related genes in Chinese cabbage when infested byMyzus persicae and Plutella xylostella. Four-week-old Chinese cabbagewas exposed to each insect for 24 h, and then proteins and total RNA were extracted from the leaves. To elucidate the herbivore-induced differentially expressed proteins in Chinese cabbage, proteins were separated by two-dimensional gel electrophoresis, and visualized by staining with Coomassie G250. Approximately 1600 protein spotswere separated, of which nearly 160 showed reproducible changes in expression. Among these, nine proteins whose expressions were markedly up-regulated in the M. persicae-infested group were identified usingmatrix-assisted laser desorption/ionization time of flight mass spectrometry. The identified herbivore-responsive proteins (ribulose-1,5-bisphosphate carboxylase/oxygenase, ATP synthase CF1, putative mismatch binding protein Mus3, and integrase core domaincontaining protein) were involved in regulation of photosynthesis, carbohydrate metabolism and DNA repair. The expression levels of chitinase, β-1,3-glucanase, peroxidase, PR1, and PR4 in herbivore-infested Chinese cabbage were also analyzed by quantitative real-time PCR.
구현나,김슬기,이재선,강원진,조우성,경예진,서진원,김현경,김길하,조수원 한국곤충학회 2017 Entomological Research Vol.47 No.3
Pseudococcus longispinus, a notorious cosmopolitan pest species of mealybugs, known to be distributed indoors in Korea since 2002, is found to be mixed with another species, Pseudococcus orchidicola. Finding P. orchidicola as a pest of tropical plants in Korea is rather unexpected because of their main distribution in the Pacific area and South Asia. However, all the available information from morphology, molecule and advice on identification from mealybug specialists indicates this is the best match for P. orchidicola. Morphological, molecular and some biological notes on P. orchidicola are provided with some adult and nymphal images, and compared with P. longispinus. A full discussion mainly on identity and distribution of P. orchidicola is given.
Pseudococcus viburni (Signoret) (Hemiptera: Pseudococcidae) does not occur in Korea
구현나,정대훈,김슬기,경예진,김길하,조수원,Koo, Hyun-Na,Jeong, Dae-Hoon,Kim, Seulki,Kyung, Ye Jin,Kim, Gil-Hah,Cho, Soowon Korean Society of Applied Entomology 2017 한국응용곤충학회지 Vol.56 No.4
가루깍지벌레류인 Pseudococcus viburni Signoret (Hemiptera: Pseudococcidae)는 포도원과 다른 과실 과원에도 피해를 주는 국제적 해충이다. 이 종은 한국에서 오랫동안 격리되어 왔으며 야외에서의 발생기록은 전혀 없다. 본 연구에서는 한국에서의 P. viburni 부재를 확인하기 위해 전국적으로 감 과수원 533사이트, 사과 및 기타 과수원 144사이트, 묘목원 및 온실 281사이트를 지난 3년간(2015-2017) 조사하였다. 그 결과 P. viburni는 한국의 야외에서나 묘목원 등에서 발생되지 않는 것으로 확인되어 이를 보고한다. The obscure mealybug, Pseudococcus viburni Signoret (Hemiptera: Pseudococcidae) is a polyphagous cosmopolitan pest, damaging vineyards and fruit orchards. The species has long been quarantined in Korea and has never been recorded in the wild in Korea. In this study, to confirm the absence of P. viburni in Korea, we have searched for the species from the orchards. 533 sites of persimmon orchards, 144 sites of apple and other orchards, and 281 sites of nurseries and greenhouses were surveyed for the last three years (2015-2017). As a result, P. viburni occurs neither in the wild nor in nurseries in Korea.
구현나,Gil Hah Kim,Seung-Hwan Yoon,Youn-Ho Shin,Changmann Yoon,Jong-Suk Woo 한국응용곤충학회 2011 Journal of Asia-Pacific Entomology Vol.14 No.3
The effect of electron beam irradiation on each developmental stage of diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae),was examined. Eggs, larvae, pupae, and adultswere irradiated at target doses of 30,50, or 100 Gy or they were left untreated as controls in replicated experiments. When eggs and pupae were irradiated with 100 Gy, emergence rates greatly decreased and, although some adults laid eggs, they did not hatch. Egg hatching of irradiated larvae and adult decreased with increasing irradiation doses. However, electron beam irradiation did not kill P. xylostella directly. Adult longevity was not affected. Reciprocal crosses between irradiated and unirradiated moths demonstrated that females were more radiosensitive than males in hatchability; however, the difference was not significant. In addition, electron beam-irradiated larvae showed typical DNA fragmentation in a dose-dependent manner compared with cells from unirradiated larvae. Our findings suggest that electron beamirradiation induces abnormal development and reproduction of P. xylostella;therefore, it may contribute to effective disinfestation and quarantine treatments of P. xylostella.
구현나,배성민,우수동 한국잠사학회 2016 International Journal of Industrial Entomology Vol.32 No.2
The major structural proteins of porcine reproductive and respiratory syndrome virus (PRRSV) are derived from ORFs 4, 5, and 6. They have been considered very important to arouse the humoral and cellular immune responses against PRRSV infection and proposed to be the excellent candidate proteins in the design of PRRS bioengineering vaccine. However, the PRRSV structural proteins are produced in low levels in the infected cells because it forms insoluble protein and possesses several transmembrane regions. To overcome this problem, we fused the ORF4, ORF5, and ORF6 with SUMO (small ubiquitin-related modifier). The resulting fusion protein SUMO-ORF4, -ORF5, and -ORF6 were highly expressed in Bm5 cells. The level of protein expression using the Bombyx mori larvae was higher than that using Bm5 cells. In addition, fusion to SUMOstar, which is not processed by native SUMO proteases, significantly enhanced protein expression levels compared to SUMO fusion. This study demonstrated that SUMO or SUMOstar, when fused with PRRSV structural proteins, was able to promote its soluble expression. This may be a better method to produce PRRSV structural proteins for vaccine development.
구현나,배성민,Jae Bang Choi,신태영,Bit Na Rae Yun,최재영,이광식,노종열,제연호,진병래,Sung Sik Yoo,김재수,In Joong Yoon,우수동,Young In Kim 한국응용곤충학회 2011 Journal of Asia-Pacific Entomology Vol.14 No.1
To characterize the NYJ strain of pseudorabies virus (PRV; Alphaherpesvirus of swine) isolated from the serum of an infected swine in Korea, the nucleotide sequence of three major glycoproteins (gB, gC, and gD)was analyzed. The expression of most potent immunogenic glycoprotein (gD) was also investigated using a Bombyx mori nucleopolyhedrovirus (BmNPV) expression system. The length of the glycoprotein genes corresponding to gB, gC, and gD of the NYJ strain were 2751 bp, 1443 bp, and 1203, respectively, and their identity ranged from 94.2% to 99.8% when compared with other strains. Phylogenetic analyses using these sequences showed that the NYJ strain forms a distinct branch with high bootstrap support. A novel transfer vector (pBmKSK4) was engineered with the polyhedrin promoter of BmNPV and a 6xHis tag to express glycoprotein gD in Bm5 cells and silkworm, B. mori, larvae. The immunogenicity of recombinant gD was demonstrated by its specific detection in both Bm5 cells and silkworm larvae by porcine anti-PRV antibody. The results of this study have implications both for the taxonomy of Korean PRV strains and vaccine development.
복숭아혹진딧물 가해에 의한 무당벌레의 유인과 배추의 간접방어
구현나,김길하 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04
무당벌레 성충은 복숭아혹진딧물이 가해한 배추에 유인이 되었으며 배추와 복숭아혹진딧물 단독에는 유인되지 않았다. 복숭아혹진딧물의 접종 밀도별 유인검정 결과, 60마리의 복숭아혹진딧물을 배추에 접종 시 무당벌레 성충의 유인율은 70.3%로 가장 높았다. 또한 인위적인 상처를 입힌 배추에는 성충과 유충 모두 유인되지 않았다. 배추에 복숭아혹진딧물 접종 후, 시간에 따른 무당벌레 성충의 유인검정결과, 60마리를 접종 시 24, 48시간 때에 유인되었고, 90마리를 접종 시 12시간 때에 유인되었다. 배추좀나방 유충에 의해 가해당한 배추에 대해 무당벌레 성충은 유인되지 않았다. 이러한 결과는 해충 가해에 의해 식물에서 방출되는 휘발성물질이 특정 해충에 대한 천적을 유인하는 것으로 설명될 수 있다. 따라서 복숭아혹진딧물에 의해 배추에서 방출되는 휘발성성분을 분석하고자 시도하였으나 실패하였다. 복숭아혹진딧물과 배추좀나방에 의한 배추에서의 단백질발현 변화를 보기위해 2D-gel electrophoresis를 수행하였으며 160개 정도의 유의성 있게 변화를 보이는 spot을 detection하였다. 또한 복숭아혹진딧물과 배추좀나방이 가해한 배추에서의 chitinase, β-1,3-glucanase, peroxidase, PR1, 그리고 PR4 등의 방어관련 유전자발현 변화를 분석하였다.
구현나,윤승환,김현경,김길하 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
이온화에너지(Ionizing irradiation)는 검역대상 병해충의 소독에 있어 각광받고 있는 비화학적 소독기술 중 하나이다. 현재 선진국에서는 감마선, X-선, 전자빔 등을 이용하여 화훼류, 과일, 채소, 저장 곡물의 살충작용 뿐만 아니라 식품과 의료품의 살균, 종자발아 억제 등에 널리 사용되고 있다. 본 연구에서는 이온화에너지에 의한 곤충의 발육과 생식에 미치는 영향을 조사하였고 어떤 기작을 통해 불임을 유발하는지 관련 메커니즘을 규명고자 하였다. 그 결과, 이온화에너지에 의해 성충 수명에는 영향이 없었고 부화율, 우화율, 용화율, 그리고 F1세대의 발육 및 생식에는 조사선량이 증가할수록 무처리 대조군에 비해 비정상적인 발육과 불임현상을 보였다. 불임 메커니즘 규명을 위해 먼저 난황단백질의 발현에 초점을 맞추어 연구하였고 또한 날개 형태기형에 관여하는 유전자 발현정도를 조사하였다. 또한 약제 저항성을 지닌 해충에 이온화에너지가 미치는 영향도 조사하였다.