Helicobacter pylori cagE 유전자 다형성이 위상피세포주에서 Interleukin-8 유도능에 미치는 영향

이수민,이학성,이인석,조유경,한혜원,오정환,박재명,최명규,정인식,Lee Su-Min,Lee Hak-Sung,Lee In-Suk,Co Yu-Kyung,Han Hae-Won,Oh Jung-Hwan,Park Jae-Myung,Choi Myung-Gyu,Chung In-Sik 한국생명과학회 2004 생명과학회지 Vol.14 No.6

Helicobacter pylori infection is highly prevalent, as high as 2/3 of whole population infected, in Korea. H. pylori infection initiates inflammation by induction of interleukin-8 through type IV secretion of CagA. It was recently suggested that induction failure of IL-8 is not associated with defect in cag PAI but associated with cagE locus diversity. This study was designed to investigate ability of 11-8 in-duction according to sequence variation within the cagE gene, cagA TP motifs and vacA m-types in vitro study using AGS cell-line, and to evaluate its association with different clinical outcome. Seventy-four H. pylori stains were isolated from 23 patients with gastric cancer (Ca), 24 subjects with gastritis (G) and 27 patients with duodenal ulcer (Du) in Kangnam St. Mary's Hospital, Seoul, Korea. cagE gene diversity was confirmed by the PCR-RFLP methods with MboI/NlaIII and tyrosine phosphate motifs (TPMs) of cagA was determined TPM-A and C by using DdeI/Tsp5091 restriction enzyme and TPM-B was determend by Real time PCR the method of Owen et al. and IL-8 was measured by ELISA assay. IL-8 activity was positively detected in 59 among 74 strains $(79.7\%)$. IL-8 secretion was significantly increased in MboI A and MboI B type compared to MboI C type and in MboI/NlaIII A-C and B-C type than C-C type. 1L-8 activity was not associated with either the number or composition of cagA tyrosine phosphorylation motifs and vacA m-type. There was no significant difference in IL-8 activity among patient groups. cagE gene diversity is thought to be mainly associated with the induction of IL-8 in H. pylori infection. 한국에서 분리한 Helicobacter pylori 균주가 위암 세포주에서 IL-8를 유도하는 유도능을 관찰하고자 하였으며, 특히 4형 분비구조의 주요 구성체인 cagE 유전자의 다형성에 따라 IL-8 유도능의 차이 및 각 질환에서 분리한 균주에서의 차이가 있는지 검토하고자 하였다. 강남성모병원 내시경검사를 받은 환자들 중 H. pylori 감염 양성으로 확인된 위암 23명 $(31.0\%)$, 위염 24명$(32.6\%)$, 십이지장궤양27명$(36.4\%)$ 총 74명을 대상으로 하여 H. pylori를 분리한 후 PCR-RFLP방법을 이용하여 cagE 다형성을 확인하였으며, PCR 및 Real-time PCR을 이용하여 ragA TPMs를 결정하였다, 또한 분리된 균주를 위암세포주에 감염시켜 상등액을 취하여 ELISA방법으로 IL-8을 정량화 하였다. 전체 74개의 분리 균주 중 59개의 균주$(79.7\%)$에서 IL-8 유도능이 있는 것으로 확인되었으며, cagE 다형성 중에서 가장 높은 빈도로 관찰되는 형태는 MboI/NlaIII B-C, A-C 및 C-C 타입 이었으며, 이중 A-C와 B-C 형태가 C-C 형태에 비해 유의적으로 높은 IL-8 유도능을 나타냈다. 그러나 cagA TPMs의 분류 및 vacA m-아형 분류와 IL-8 유도능과는 연관성이 없었다. 비록 본 연구에서 cagE 다형성과 IL-8 유도능과 아주 밀접한 연관성이 나타나지는 않았지만 vacA m-아형이나 cagA TPMs 보다는 cagE 다형성이 IL-8 유도능과 연관성이 클 것으로 생각된다.

Bayesian Estimation for the Weibull Model under the Progressively Censoring Scheme

이인석,조길호,채현숙,Lee, In-Suk,Cho, Kil-Ho,Chai, Hyeon-Suk Korean Data and Information Science Society 1991 한국데이터정보과학회지 Vol.2 No.-

The maximum likelihood estimators and Bayes estimators of the parameters and reliability function for the two-parameter Weibull distribution under the type-II progressively censoring schemes are derived when a shape parameter is known and unknown, respectively. Efficiencies for above estimators are also compared each other in terms of the mean square errors, and Bayes risk sensitivities of the Bayes estimators are investigated.

잔차 분산을 이용한 선형회귀모형의 다중전환점 검정

이인석,김종태,이금자,Lee, In-Suk,Kim, Jong-Tae,Lee, Kum-Ja 한국데이터정보과학회 2001 한국데이터정보과학회지 Vol.12 No.1

The purpose of this study is to test a multiple change point in the regression model with the passage of time, using the estimated residual variance figure suggested by Gasser, Sroka and Jennen - Steinmez (GSJS). As a result of the simulation, it is showed that there is a jump change of the estimated residual variance figure at that time of change point. The way to analyse a intuitive multiple change point through graphics is more effective and accurate than any other existing ways. 본 연구는 시간의 변화에 따라 여러 개의 전환점이 발생하여 선형회귀모형들이 여러번 변화할 때의 변환시점을 Gasser, Stroke와 Jennen-Steinmez의 잔차분산 추정량을 이용하여 검정하고 실제의 몇 가지 모형을 제시하여 Graphic을 통하여 조사한 결과 여기서 제시한 방법이 더 효과적으로 자중전환점을 찾을 수 있었다.

Simultaneous Estimation of Several Poisson Means under a Linex Loss Function

이인석,정원태,정혜정,Lee, In-Suk,Jeong, Won-Tae,Jeong, Hye-Jeong The Korean Data and Information Science Society 1993 한국데이터정보과학회지 Vol.4 No.-

We find a class of admissible Bayes estimator for the mean vector ${\theta}=({\theta}_{1},{\theta}_{2},...,{\theta}_{p}$ of Poisson distribution under a LINEX loss function. The Monte Carlo Simulation is performed to compare the emprical Bayes estimater under the LINEX loss function and weighted squared error loss respectively.

On Jacknife Reliability Estimation in the Weibull Case

이인석,금윤희,Lee, In-Suk,Keum, Yoon-Hee The Korean Data and Information Science Society 2002 한국데이터정보과학회지 Vol.13 No.2

We compare MISE of the MLE, UMVUE, invariantly optimal estimator and Jacknife estimator for the reliability function of the Weibull distribution when the sample size is small.

연구논문 : 다중오류들을 갖는 소프트웨어 신뢰성의 추정

이인석,정원태,정혜정 ( In Suk Lee,Won Tae Jung,Hye Jeong Jeong ) 한국품질경영학회 1995 품질경영학회지 Vol.23 No.3

한반도 동해안 밍크고래 체내 미량금속 잔류특성

전유영,황동운,이인석,최민규,김숙양,이석모,Jeon, You-Young,Hwang, Dong-Woon,Lee, In-Seok,Choi, Min-Kyu,Kim, Sook-Yang,Lee, Suk-Mo 해양환경안전학회 2012 해양환경안전학회지 Vol.18 No.6

To determine the concentration of trace metals in the tissues of whale from the coastal ocean of Korea, we measured the concentration of trace metals (Cu, Cd, Pb, As, Zn and Hg) in the organs (liver, intestine, muscle, epidermis and blubber) of minke whale entangled from the eastern coast of Korea in 2009. The highest and lowest concentrations of Cu, Cd, Zn, and Hg were found in the liver and blubber, respectively. In contrast, the highest and lowest concentration of As was found in the blubber and muscle, respectively. The accumulation trend of trace metals in the organs of minke whales was in the order Zn > Cu > As > Pb > Hg > Cd. The concentrations of Cu, As and Zn in liver and epidermic were higher in male than in female, whereas the concentrations of Cu and Pb in intestine and muscle were higher in female than in male. The mature individuals of minke whale were much higher Cd in liver, Cd and Hg in muscle, As and Hg in epidermic and blubber, Cd, As and Hg in intestine than immature individuals of minke whale. 한반도 연안에 서식하는 고래의 조직 내 미량금속 농도 잔류특성을 알아보기 위하여 2009년 동해안에서 혼획된 밍크고래의 간, 창자, 근육, 표피, 지방 내 미량금속(Cu, Cd, Pb, As, Zn, Hg) 농도를 측정하였다. 조직 내 Cu, Cd, Zn, Hg은 간에서 가장 높고 지방에서 가장 낮았다. 이와 대조적으로 As는 지방에서 가장 높은 농도를 보였으며, 근육에서 가장 낮은 농도를 나타내었다. 전반적으로 밍크고래의 조직 내 미량금속은 Zn > Cu > As > Pb > Hg > Cd의 순으로 높은 농도를 나타내었다. 성별에 따른 미량금속은 수컷은 간과 표피에 Cu, As, Zn을, 암컷은 창자와 근육에 Cu와 Pb을 더 효율적으로 축적하고 있는 것으로 나타났다. 성숙도에 있어서는 간의 경우 Cd, 근육은 Hg, 표피와 지방은 As와 Hg, 창자는 Cd, As, Hg이 성숙한 개체에서 미성숙한 개체보다 상당히 높은 농도를 보였다.

역류식도염 환자에서 Helicobacter pylori 감염과 Gastrin, Pepsinogen의 관계 -역류식도염과 H. pylori 감염, 위축위염의 관계-

권정현 ( Jung Hyun Kwon ),정인식 ( In Sik Chung ),손혜숙 ( Hye Suk Son ),박재명 ( Jae Myung Park ),조유경 ( Yu Kyung Cho ),이인석 ( In Seok Lee ),김상우 ( Sang Woo Kim ),최명규 ( Myung Gyu Choi ) 대한소화기학회 2008 대한소화기학회지 Vol.51 No.3

목적: H. pylori 감염은 위축 위염의 중요한 원인이 되며, 혈청 gastrin과 pepsinogen (PG) 분비에도 영향을 미친다. 위식도역류질환 환자에서 H. pylori의 빈도가 낮다는 보고들이 있으나 위식도역류질환의 발생과 H. pylori 감염의 관계는 아직도 분명치 않다. 이번 연구에서는 미란 식도염(erosive reflux esophagitis, ERD)의 원인인자인 위산 분비와 관련이 있는 H. pylori 감염과 gastrin, PG I, II 및 I/II 비와의 관계를 알아보고자 하였다. 대상 및 방법: 환자 2006년 3월부터 10월까지 강남성모병원에서 한 명의 내시경 전문의가 상부위 장관 내시경 검사로 ERD로 진단한 환자를 대상으로 하였고 나이와 성을 대응시켜 대조군을 선정하였다. 두 군 모두 내시경 검사에서 궤양 등의 다른 위장관 질환이 있거나 제균 치료를 받은 자는 제외하였다. 음주와 흡연 유무, 신체질량 지수(BMI)를 조사하였고 H. pylori 감염, 혈청 PG I, II (라텍 스응집면역비탁법)와 gastrin (방사면역측정법)을 검사하였다. 결과 총 45명의 ERD 환자군과 66명의 대조군을 비교하였다. H. pylori 감염은 ERD군에서 대조군보다 낮았다(11.1%vs. 43.9%, p<0.05). PG I/II 비는 ERD군에서 대조군보다 높았다(7.0±3.1 vs. 5.3±2.6, p<0.05). PG II와 gastrin은 ERD군에서 대조군 보다 낮았다(p<0.05), BMI는 ERD군이 대조군보다 높았다(24.46 kg/m2 vs. 23.08 kg/m2, p<0.05). 결론: 한 국인에서 ERD 환자군은 대조군보다 H. pylori 감염률이 낮고, PG I/II 비가 증가되어 있어 역류식도염은 위점막의 위축이 더 적은 환자에서 잘 생기는 것으로 생각한다. PG I/II비는 H. pylori 감염 검사와 함께, ERD 환자군에서 동반되어 있는 위축 위염의 형태나 정도, 위산 분비 정도를 예측해 볼수 있는 인자로서 유용성이 있다고 생각한다. Background/Aims: Helicobacter pylori (H. pylori) infection is known as a major cause of atrophic gastritis and is associated with serum gastrin, pepsinogen, and gastric acid secretion. There is still a controversial association between gastroesophageal reflux disease and H. pylori infection. This study was designed to investigate the relationship among serum gastrin, pepsinogen, and H. pylori infection in the erosive reflux esophagitis (ERD) patients. Methods: Patients who were diagnosed as ERD by one gastroenterologist at the Kangnam St. Mary`s hospital were prospectively enrolled. The persons without ERD in the control group were matched for age and sex. We examined the gastrin, pepsinogen I (PG I), PG II, PG I/II ratio, and H. pylori infection. Results: Forty five patients were enrolled in ERD group and 66 persons in control group. The H. pylori infection rate in ERD group was lower than that in the control group (11.1% vs. 43.9%, p<0.001). PG I/II ratio in ERD group was higher than that in the control group (7.0±3.1 vs. 5.3±2.6, p=0.003). The PG II (p=0.016) and gastrin (p=0.029) in ERD group were lower than those in the control group. BMI in ERD group was higher than that in the control group (24.5 vs. 23.1 kg/m2, p=0.013). Conclusions: The H. pylori infection rate in ERD group was lower and PG I/II ratio was higher than that in the control group. Reflux esophagitis is thought to be reversely associated with the atrophy of gastric mucosa. (Korean J Gastroenterol 2008;51:159-166)

개인-환경적합성이 조직유효성에 미치는 영향에 관한 연구

이인석(Lee, In-Suk),백종훈(Baek, Jong-Hoon),전무경(Jeon, Moo-Kyeong) 대한경영학회 2010 大韓經營學會誌 Vol.23 No.4

남북경협 현황과 활성화 방안

이인석 ( In Suk Lee ) 아시아.유럽미래학회 2006 유라시아연구 Vol.3 No.1

Since 1989, economic relation between two Koreas has been rapidly increased in term of trade and investment as well, mainly drived by government of South Korea. As a result, South Korea is the 2th largest trade partner for North Korea, next to China and economic dependency on South Korea is also getting higher. Trade with South Korea is one of important sources of earning foreign currency for North Korea. However, lack of marketable products of north korea to export to south korea makes it difficult for both sides to expand economic exchange and cooperation. Development of economic relation between two Koreaa relys substantially on non-commercial transaction in form of economic aids by South Korea. For enhancement of economic relation with north korea, efforts are needed to promote exchange of products. As for investment in North Korea, business climate is underdeveloped so that privite companies invest hardly with firm expectation of gaining profits. Under abnormal situation facing North Korea, it is desirable to put first priority on promoting transaction of products by privite companies. In parallel with it, efforts should be concentrated on making investment climate more sound, for example the development of Gaesung Industrial Complex. Besides to promoting the bilateral relation, cooperation with neighboring countries will make great contribution to promotion of economic relations between two Koreas. However, confrontation between North Korea and the U.S is main obstacle to pave the way for multilateral cooperation, which makes North Korea more isolated from the rest world. If current issues like nuclear problem would not find solution, North Korea``s isolation would be hardly overcome, which would also make difficult further development of economic cooperation between two Koreas It is remarkble that trade between North Korea and China is expanding with galoping tempo. Trade between two countries in 2005 increased by 40% comparing previous year, which is faster than that between two Koreas. China is the 1st trade partner for North Korea. Political leaders of China who visited Pungyang last year gave words to cooperate with North Korea for economic development. Nowdays China is first largest trade partner for both North- and South Korea. Interdependency of three countries will be more deepening. China has grand plan to establish the economic zone in the Northeast Asia including North Korea. Tripartite relation among China, ROK, DPRK will play favourable role in developing economic relation of two Koreas.