생물반응기 배양을 통한 두릅나무(Aralia elata)의 체세포배 및 유식물체 대량증식

이원석,최은경,김재훈,Lee, Won-Seok,Choi, Eun-Gyung,Kim, Jae-Whune 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.3

두릅나무의 엽병을 1.0 mg/L 2,4-D가 포함된 MS 고체배지에서 배발생캘러스를 유도하였다. 배발생세포와 배발생세포괴는 1.0 mg/L 2.4-D가 포함된 MS 액체배지에서 배발생캘러스를 2주간 현탁배양하여 대량으로 얻었다. 그물망을 통과한 배발생세포는 1.0 mg/L 2.4-D가 포함된 MS 액체배지에서 배양하면 배발생능이 소실되지 않고 지속적으로 유지 및 증식시킬 수 있었다. 그물망을 통과하지 못한 배발생세포괴를 식물생장조절물질이 첨가되지 않은 1/2 MS 액체배지에 옮겨 2주간 배양하면 구상형의 체세포배로 발달하였다. 구상형의 체세포배는 5 L의 bioreactor를 이용하여 배양하면 심장형, 어뢰형, 자엽형의 배와 유식물체로 발달하였다. Bioreactor 배양을 통해 두릅나무의 체세포배를 효과적으로 대량증식 시킬 수 있었다. Embryogenic calli were induced from petioles of Aralia elata on MS solid medium supplemented with 1.0 mg/L 2,4-D. When embryogenic calli were transferred to MS liquid medium supplemented with 1.0 mg/L 2,4-D, embryogenic cells and embryogenic cell clusters were developed after 2 weeks of culture. Embryogenic cells were filtered through a 250 ${\mu}{\textrm}{m}$ sieve and the passed cells were proliferated and maintained in MS liquid medium supplemented with 1.0 mg/L 2,4-D. Embryogenic cell clusters entrapped on the sieve were transferred to 1/2 MS liquid medium without plant growth regulators, globular-shaped embryos were developed from embryogenic cell clusters after 2 weeks of culture. Numerous early stage somatic embryos could be developed to heart-shaped, torpedo-shaped, cotyledonary embryos and plantlets in 5 L bioreactor. Above results suggest that effective somatic embryo proliferation can be achieved via bioreactor culture systems in Aralia elata.

Special Report - W3C 오토모티브 웹(Automotive Web) 표준화 현황

이원석,이승윤,Lee, Won-Seok,Lee, Seung-Yun 한국정보통신기술협회 2016 TTA저널 Vol.166 No.-

국내외 네칸만화 수사적 표현 연구

이원석,Lee, Won-Seok 한국만화애니메이션학회 2005 만화애니메이션연구 Vol.- No.9

이 연구는 국내외 네칸만화에서 사용된 수사적 표현이 이야기 전개에서 어떻게 구현되는지 고찰한다. 수사라는 것이 말을 정갈하게 다듬는 과정이라면, 만화에서 수사는 그림의 스타일과 이야기의 전개를 보다 읽기 쉽고, 재미있게 만드는 데 목적이 있다. 네칸만화는 장편 만화의 서사구조를 따른다. 기승전결로 전개되는 기본적인 구조를 갖는데, 이때 수사적 표현이 동원된다. 수사적 표현에는 연역적 ${\cdot}$ 비연역적 전개를 주로 다룬다. 국내외 유명 작품의 수사적 표현의 분석을 통해 네칸만화가 가진 이야기 구조를 밝히는데 목적이 있고, 시사만화와 유머만화에서 사용된 수사적 표현의 차이점을 밝히는 기회도 된다. Comic has a story. Comic exits as a some kind of media to convey the story the writer wants to say and it not only is a message described by sign, content, and complexity but also works in more vast communication condition. This Study treats that the rhetoric expression of 4 panels comicstrip in internal and external weigh with the narration of story. If the rhetoric is the sort out of the words, in the rhetoric of 4 panels comicstrip, the target is how easy to read, how interesting to read. Here are the analysis of the narrative structure in daily life and current affair 4 Panel Comic Strips. To analyze and compare the narrative structure of two above strips proposes actual model of basic skill making 4 Panel and further will be applied in the narrative structure of long comics. The study of explaining the feature of 4 Panel Comic Strips is proposed, based on the basic act in all comics. The narrative structure of the 4 Panel Comic strips Comic Strips has the basic structure of long serialized comics and story telling.

표준·시험인증 기술 동향 - W3C 핵심 표준 개발 동향

이원석,이승윤,Lee, Won-Seok,Lee, Seung-Yun 한국정보통신기술협회 2016 TTA저널 Vol.165 No.-

1.75D 초음파 트랜스듀서의 설계 및 제작

이원석,노용래,Lee, Wonseok,Roh, Yongrae 한국음향학회 2013 韓國音響學會誌 Vol.32 No.3

In this paper, a $64{\times}8$ channel 1.75D ultrasonic transducer made of piezoelectric single crystals was designed, fabricated, and evaluated. First, a structure of the transducer was selected to be suitable for wiring on a planar array, and components were fabricated to correspond to the structure. Detailed structure of the transducer was designed through finite element analyses. As main performance factors, the crosstalk between neighboring elements was reduced through the control of kerf width and material, and desired frequency bandwidth of the transducer was achieved by designing the optimal thicknesses of the piezoelectric single crystal and matching layers. An experimental prototype of the transducer was fabricated following the design, and its performance was measured. Then the experimental results were compared with those of the finite element analysis, which led to the evaluation of the transducer developed in this work. 본 논문에서는 압전단결정으로 구성된 $64{\times}8$ 채널 1.75D 초음파 트랜스듀서의 설계, 제작 및 평가를 하였다. 우선, 평면 배열된 독립소자에 전기적 연결이 가능한 트랜스듀서의 구조를 선정하고 구조에 맞게 구성소자를 제작하였다. 그리고 유한요소 해석을 통해 트랜스듀서의 세부 구조를 설계하였다. 트랜스듀서의 주요 성능요소로서, 치폭의 너비와 재료를 조절하여 소자간 상호간섭의 영향을 줄이고, 압전단결정 및 정합층의 최적 두께를 설계함으로써 트랜스듀서의 원하는 대역폭을 구현하였다. 설계에 맞게 트랜스듀서 시편을 제작하고 실험적으로 그 특성을 측정한 후, 그 결과를 유한요소 해석 결과와 비교함으로써 개발된 트랜스듀서의 성능을 평가하였다.

정신분열병의 CDH2 유전자 다형성

이원석,김미경,정한용,우성일,권영준,김종우,이희제,Lee, Won Seok,Kim, Mi Kyung,Jung, Han Yong,Woo, Sung Il,Kwon, Young Joon,Kim, Jong Woo,Lee, Hee Je 대한생물정신의학회 2005 생물정신의학 Vol.12 No.1

Objective:There has been increasing evidence that neurodevelopmental dysfunction is involved in the pathophysiology of schizophrenia. Cadherin is known to be one of the important molecules in neurodevelopment. This study was performed to examine the relationship between T816C polymorphism of CDH2 gene and schizophrenia. Methods:Genoytypes of T816C polymorphism of CDH2 gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 156 Korea patents with schizophrenia and 170 controls. Results:No difference was found between the patients with schizophrenia and the controls in genotype and allele frequencies of T816C polymorphism of CDH2 gene. Conclusion:The results of this study do not support an association between T816C polymorphism of CDH2 gene and schizophrenia. However, it is necessary to investigate other polymorphic regions of CDH2 in schizophrenia.

의료용 초음파 위상배열 트랜스듀서의 열 분산 방안

이원석,노용래,Lee, Wonseok,Roh, Yongrae 한국음향학회 2015 韓國音響學會誌 Vol.34 No.3

초음파 영상 품질의 향상을 위해 트랜스듀서의 구동전압을 높이게 되면 트랜스듀서에서 열이 발생하여 환자의 피부 화상 및 트랜스듀서의 성능 저하를 초래할 수 있다. 따라서, 이렇게 온도가 상승하지 않도록 트랜스듀서 내의 열을 효율적으로 분산할 수 있는 방안에 대해 연구하였다. 이때 열 분산 해석 대상으로 중심주파수가 3 MHz이고 32채널을 가진 위상배열 트랜스듀서를 선정하였다. 먼저 트랜스듀서의 동작에 따른 발열 구조를 이론적으로 분석하였고, 그 결과로서 재료의 감쇠와 음압의 크기가 발열에 영향을 미치는 것을 확인하였다. 나아가 유한요소 해석을 통해 트랜스듀서 구성소자의 물성이 열 분산에 미치는 영향을 분석하였다. 분석된 결과를 바탕으로 트랜스듀서 내 열이 잘 분산되기 위한 구성소자의 열물성을 정하였다. 도출된 열 물성을 유한요소 해석 모델에 적용한 결과 환자와 접촉되는 부분인 음향렌즈의 최고온도가 원래 값의 51 %로 저하되었다. When the driving voltage of an ultrasound transducer is increased to improve the quality of ultrasound images, heat is generated inside the transducer that can cause patient's skin burn and degradation of transducer performance. Hence, in this paper, a method to disperse the heat of the transducer has been studied. The phased array transducer having 3 MHz center frequency and 32 channels was selected for analyses of the thermal dispersion. First, mechanism of the heat generation was investigated in relation to the transducer operation through theoretical analysis, and material damping and sound pressure amplitude were confirmed to be influential on the heat generation. Further, we investigated the effects of the properties of the materials constituting the transducer on the thermal dispersion through finite element analysis. Based on the analysis results, we determined the thermal properties of the constituent materials that could facilitate the thermal dispersion inside the transducer. The determined thermal properties were applied to the finite element model, and the results showed that the maximum temperature at an acoustic lens contacting with a patient was decreased to 51 % of its initial value.

Special Report - 블록체인과 DLT 상호운용성 표준 및 기술 동향

이원석,이강찬,Lee, Won-Seok,Lee, Gang-Chan 한국정보통신기술협회 2018 TTA저널 Vol.2018 No.-

수평사시 환아에서 수평직근후전술의 수술 결과에 대한 근시 및 안축장 길이의 영향

이원석,이종복,한승한,Won-Seok Lee,Jong-Bok Lee,Seung-Han Han 대한안과학회 2011 대한안과학회지 Vol.52 No.4

Purpose: To assess the influence of axial length for surgical outcome of horizontal recti recession in children with horizontal strabismus. Methods: A retrospective, non-comparative case series. 100 patients, 96 males and 44 females with unilateral or bilateral high myopia and strabismus surgery was performed. All patients had horizontal recti recession and no combined vertical strabismus. There was no history of any ocular trauma or surgery and no history of any other systemic diseases that affect ocular motility. After horizontal recti recession, the patients were followed-up for more than one year. Data was analyzed using SPSS Software for Windows, version 12.0.1. Results: In 100 children, the mean axial length was 23.21 ± 1.38 mm (range, 19.63-26.50 mm) and the mean age was 7.43 ± 2.86 years (range, 1-14 years). Lateral rectus recession was in 89 cases, and medial rectus recession was done in 11 cases. After operation, 86 cases were fully corrected, 11 cases were under corrected and 3 cases were over corrected. Binary logistic regression analysis was performed revealing that, preoperative angle of deviation (p-value 0.062) and axial length (p-value 0.05) were highly correlated with surgical outcome. Conclusions: Age, preoperative angle of deviation, and axial length may influence the surgical outcome of horizontal recti recession in children. In eyes with long axial length, the surgical outcome of horizontal recti recession appears to be poorer than that in eyes with short axial lengths. Adjusting the recession amounts when performing horizontal recti recessions in long axial length eyes may provide better results. J Korean Ophthalmol Soc 2011;52(4):472-476

WS-ECA 프레임워크 설계 및 구현

이원석,신동민,이규철,Lee, Won-Suk,Shin, Dong-Min,Lee, Kyu-Chul 한국정보통신학회 2007 한국정보통신학회논문지 Vol.11 No.8

유비쿼터스 환경으로 발전하면서 이종의 디바이스들을 자연스럽게 연동시킬 수 있는 기술에 대한 요구가 크게 증가하고 있으며, 이를 해결하기 위한 하나의 대안으로 웹서비스가 주목받고 있다. 최근 디바이스 연동을 선언적인 ECA(Event-Condition-Action) 룰로 정의하고 이를 이용하여 디바이스 연동을 수행할 수 있는 웹서비스 기반의 WS-ECA(Web Services-ECA) 프레임워크가 제안되었다. 본 논문에서는 WS-ECA 프레임워크에 대한 구체적인 모듈 단위의 설계 부분을 제안하고, 이를 기반으로 WS-ECA 프레임워크를 구현하였다. Requirement of coordination among heterogeneous devices is significantly increasing to meet the challenges presented by the evolvement of the ubiquitous computing environment. The web services technology is receiving much attention as a promising solution to support the inter-operability between such heterogeneous devices by means of well-defined protocol. Recenlty, ECA(Event-Condition-Action) rule-based framework for coordinating devices is proposed, which is called WS-ECA(Web Services-ECA) framework In this paper we propose a specific design and an implementation methodology for WS-ECA framework.