유방암 치료에 대한 한의 임상 가이드라인 개발을 위한 기초연구

이강욱,유화승,이진선,이남헌,Lee, Kang-Wook,Yoo, Hwa-Seung,Lee, Jin-Sun,Lee, Nam-Hun 대한한방부인과학회 2016 大韓韓方婦人科學會誌 Vol.29 No.2

Objectives : The purpose of this study was to present the clinical practice guidelines for breast cancer through the analysis of existing clinical practice guidelines in Western and Chinese medicine. Methods : The literature search was performed regarding conventional and integrative medical treatment of breast cancer using Google Scholar, KIOM OASIS, PUBMED and Jisan library of Daejeon university. Results : Breast cancer is the fifth most common cancer in Korea. Recent studies revealed that combining conventional and integrative medical treatment can reduce the sequela of surgical operation and improve survival rate. However there isn’t still a systemic clinical guideline for breast cancer in korean medicine. This study will be a preliminary study to establish clinical practice guidelines of Korean Medicine for breast cancer.Conclusions : Further objective and systemic clinical studies related to Korean Medicine are needed to develope more advanced clinical practice guidelines of breast cancer.

김치유산균용 발현벡터 pSJE6c 개발과 이를 이용한 외래 유전자 발현

이강욱,박지영,이지연,이황아,백창운,조현덕,김주연,권건희,천지연,김정환,Lee, Kang-Wook,Park, Ji-Yeong,Lee, Ji-Yeon,Lee, Hwang-A,Baek, Chang-Un,Jo, Hyeon-Deok,Kim, Joo-Yeon,Kwon, Gun-Hee,Chun, Ji_Yeon,Kim, Jeong-Hwan 한국미생물·생명공학회 2009 한국미생물·생명공학회지 Vol.25 No.4

본 연구실에서 개발한, 김치에서 분리한 Leu. mesenteroides SY2 유래 pFMBL1 을 바탕으로 구축한 셔틀벡터인, pSJE[7]를 외래유전자 발현에 적합하게 개량한 발현벡터를 구축하였다. Lactococcus lactis LM0230에서 분리한 프로모터 P6C를 pSJE에 도입하였다. P6C 염기서열을 지닌 oligonucleotide 쌍을 따로 제조한 후 annealing을 통해 짧은 DNA 단편을 얻어서 제한효소 처리후 pSJE에 도입하여 pSJE6c를 구축하였다. PSJE6c 효능 검증을 위해서 외래 유전자인 aga와 lacZ를 각각 pSJE6c에 도입하였다. P6C 프로모터와 비교를 위해 고유 프로모터를 지닌 유전자들도 각각 pSJE에 도입하였다. 재조합 plasmid들을 electroporation 방법으로 Lactobacillus brevis 2.14 균주에 도입하고 재조합균주들의 생육곡선과 효소역가 그리고 slot blot으로 전사체 농도를 측정하였다. 결과를 보면 PSJE6c에 클로닝 된 유전자들이 pSJE상의 유전자보다 효소역가들이 약 1.5배에서 2배 정도로 높았다. 전사체 농도 측정 결과도 pSJE6c 들에서 더 많은 전사체가 생성됨을 보여주었다. 이상 결과들은 효율적인 발현벡터들의 사용을 통해서 김치유산균에서 외래유전자 발현 효율을 높일 수 있음을 보여준다. Development of expression vectors is important for the basic and applied researches on kimchi LAB (lactic acid bacteria). An expression vector, pSJE6c was constructed by inserting P6C promoter sequence from Lactococcus lactis into pSJE, a shuttle vector for E. coli and Leuconostoc species. To test the efficiency of pSJE6c, aga ($\alpha$-galactosidase) and lacZ ($\beta$-galactosidase) genes were expressed in Lactobacillus brevis 2.14. Compared to the pSJE, expression levels of both genes were increased, indicating P6C promoter was better than indigenous promoters. Enzyme activities of L. brevis cells harboring pSJE6caga (pSJE6c with aga) or pSJE6Z (pSJE6c with lacZ) were 1.5-2 fold higher than those with pSJEaga (pSJE with aga) or pSJEZ (pSJE with lacZ). More RNA transcripts were detected in cells harboring pSJE6c based recombinant plasmid. The results indicated that heterologous gene expressions in kimchi LAB could be improved significantly by use of efficient expression vectors.

두부외상후 만성 기질성 정신장애 환자에서 Tc-99m HMPAO Brain SPECT 분석

이강욱,이종진,송민호,강민희,지익성,신영태,노흥규 ( Kang Wook Lee,Jong Jin Lee,Min Ho Shong,Min Hee Kang,Ick Sung Ghi,Young Tai Shin,Heung Kyu Ro ) 대한핵의학회 1994 핵의학 분자영상 Vol.28 No.3

It is well known that Tc-99m HMPAO brain SPECT can reflect the functional lesions better than X-ray computerized tomography(CT) and magnetic resonance imaging(MRI) in the cerebral disorders. In order to evaluate the clinical utilities of Tc-99m HMPAO brain SPECT in patients with post-traumatic chronic organic mental disorder(OMD). We included 28 patients diagnosed as OMD in department of psychiatry after traumatic head injury. And we compared the results of Tc-99m HMPAO SPECT with those of MRI, EEG and MINI mental status examination(MMSE). The results were as follows 1) All patients diagnosed as OMD showed diffuse or focal decreased cerebral perfusion on Tc-99m HMPAO SPECT. 2) Most frequent lession on brain Tc-99m HMPAO SPECT was decreased perfusion on both frontal lobe. And most frequent lesion on brain Tc-99m HMPAO SPECT was decreased perfusion on both frontal lobe. And most frequent lesion on brain Tc-99m HMPAO SPECT showing normal brain MRI result was also decreased both frontal perfusion. 3) Eight of 28 patients showed focal brain MRI lesions(4 small frontal hygroma, 3small cerebral infarction and 1 cerebellar encephalomalacia) which were not detected in brain Tc-99m HMPAO SPECT. 4) The patients showing less than 20 points on MMSE disclosed abnormal results of EEG more frequently than those disclosing more than 20points. In conclusion, we think that Tc-99m HMPAO brain SPECT is sensitive method to detect functional lesions of the brains in patients with chronic post-traumatic organic mental disoder.

임신 Rat에 있어서 방사성인(³²P)의 흡수 분포에 관한 연구

이강욱,정영채,이홍식,남치주,Lee, Kang Wook,Chung, Yung Chai,Lee, Heung Shik,Nam, Tchi Chou 대한수의학회 1969 大韓獸醫學會誌 Vol.9 No.2

The distribution of radiophosphorus ($^{32}P$) was observed in three groups of female Albino tat consisted of early pregnancy group (10 days of pregnancy), late pregnancy group (18 days of pregnancy) and nonpregnanted control group. The results obtained were as follows: 1. The various sites arranged in the descending of absorption rate of $^{32}P$ were femur, mandible, liver, spleen, ovary, fetus (only from early pregnancy group) and kidney in both groups of non-pregnanted and early pregnancy and femur, mandible, fetus, liver, ovary, spleen and kidney in the late pregnancy group. 2. The significant difference (P<0.05) in the absorption rate was found between femur and mandible in the early pregnancy group. 3. The absorption rate of fetus from late pregnancy group was significantly (P<0.01) higher than that of fetus from the early pregnancy group. 4. The high absorption rate was observed with femur and mandible in both groups of non-pregnanted and early pregnancy. 5. The significantly (P<0.01) high absorption rate was observed with ovary and fetus from the late pregnancy group.

입체복합공간 개발사업의 프로그램 성과관리 체계 구축

이강욱,홍화욱,박희대,한승헌,Lee, Kang-Wook,Hong, Hwa-Uk,Park, Hee-Dae,Han, Seung-Heon 한국건설관리학회 2011 한국건설관리학회 논문집 Vol.12 No.1

도시재생사업의 시장 규모는 꾸준히 증가하고 있으며, 도시재생 사업에서 큰 비중을 차지하는 입체복합공간 개발사업 역시 증가하는 추세를 보이고 있다. 하지만 기존의 도시재생 관련 연구는 국내외 사례의 비교분석에 기반한 시사점 및 제도의 개선방안 도출이 주를 이루어왔으며, 사업성패여부와 관련 깊은 사업관리 분야의 연구, 특히 사업관리의 기초를 제공하는 성과관리에 대한 연구는 상대적으로 미비한 실정이다. 본 연구는 입체복합공간 개발사업의 성공적인 추진을 위해 공공발주자 관점의 프로그램 성과관리 체계를 구축하는데 목적을 두고 진행되었다. 이를 위해 기존 문헌분석, 전문가 인터뷰 등을 실시하였으며, 입체복합공간 개발사업의 시설물 유형별 성과지표 및 리스크 연계 성과지표를 도출하고, 프로그램 성과점수 통합방법을 제안하였다. 본 연구에서 제안한 성과관리 체계는 기본계획 수립단계에서부터 유지관리단계까지 전 생애주기를 고려하고 있으며, 시설물 유형별 성과를 실시간으로 파악 및 평가가 가능한 구조를 갖추고 있다. 또한 리스크 연계 성과관리 체계는 주요 리스크요인별 대응주체 및 대응방안을 제공함으로써 리스크가 높은 성과지표의 지속적인 추적 및 통제를 가능하게 하였다. 본 연구는 도시재생사업의 전 생애주기에 걸친 성과관리 기술 향상을 통해 해당 분야의 효율성 제고에 기여할 것으로 기대되며, 향후 데이터웨어하우스 기반의 웹기반 성과관리시스템의 기본구조로 활용될 것이다. The market volume of urban regeneration projects has steadily increased, thereby mixed-use development also shows significant market growth. However, previous researches on urban regeneration mainly deduced suggestions and plans for institution improvement based on comparison between projects implemented the inside and outside of the country. Whereas, researches on project management closely related to project success were limited, particularly in performance management. Accordingly, this research aims at developing a program performance management framework for public clients so as to succeed mixed-use development projects. Through extensive literature review and expert interview, this research developed performance indices for diverse facilities, risk management framework and integrating method of program performance score. The proposed framework is able to consider total life cycle from basic planning to maintenance phase and to check real-time performance level. Moreover, risk management framework can periodically assess and control the level of inherent risks within performance indices. The results can contribute to improve existing performance management practices and be basis for a web-based system developed in future.

한인진(韓茵蔯), 울금(鬱金), 산사(山査), 단삼(丹蔘) 발효복합추출물이 RAW264.7 및 HUVEC 세포에서 나타나는 이상지질혈증 관련 염증인자 발현 및 항산화에 미치는 영향

이강욱,조현경,유호룡,설인찬,김윤식,Lee, Kang-wook,Cho, Hyun-kyoung,Yoo, Ho-ryong,Seol, In-chan,Kim, Yoon-sik 대한한방내과학회 2018 大韓韓方內科學會誌 Vol.39 No.4

Objectives: To investigate the effect of fermented extract of Artemisiae Iwayomogii Herba, Curcumae Longae, Crataegi Fructus and Salviae Miltiorrhizae Radix (FMH) on anti-inflammation associated with dyslipidemia and anti-oxidation in RAW264.7 and HUVEC cells. Methods: The total polyphenols, total flavonoids, DPPH radical scavenging activity, ABTS radical scavenging activity, and cytotoxicity of FMH were measured. RAW264.7 cells treated with FMH were tested for production of NO, and for cytokine and LTB4 levels and HUVEC cells treated with FMH were examined for production of cDNA of genes related to inflammation. Results: 1. FMH contained polyphenols and flavonoids. The DPPH and ABTS radical scavenging activity of FMH increased in a concentration-dependent manner. 2. FMH treatment inhibited the production of nitric oxide (NO), cytokines, and LTB4 in RAW264.7 cell when compared to the untreated control group. 3. FMH decreased the transcription of pro-inflammatory genes, whereas it increased transcription of anti-inflammatory genes, in HUVEC cells. Conclusion: FMH is effective as an antioxidant and for treatment and prevention of dyslipidemia, atherosclerosis, ischemic heart disease, stroke, and other cardiocerebrovascular diseases.

국내 토목산업의 설계-시공 균형발전 방안 도출에 관한 연구

이강욱,한승헌,박형근,진경호,Lee, Kang Wook,Han, Seung Heon,Park, Hyung Keun,Chin, Kyung Ho 대한토목학회 2013 대한토목학회논문집 Vol.33 No.1

최근 해외건설시장에서는 산업설비 분야를 중심으로 기획, 설계, 구매조달, 시공을 통합하는 EPC(Engineering, Procurement, & Construction) 형태의 발주물량이 증가하고 있으며, 토목산업 분야에서도 점차 Design-Build 발주사례가 증가하고 있다. 이러한 발주환경의 변화에 따라 프로젝트종합관리, 기본설계 등 건설엔지니어링의 중요성이 대두되고 있으나, 아직까지 국내 건설산업은 시공 위주의 산업구조에서 벗어나지 못하고 있다. 따라서 본 연구는 국내 토목산업의 엔지니어링 역량을 제고하고, 장기적으로는 설계-시공 균형발전을 위한 산업 차원의 발전방안을 제시하는데 목적을 두고 진행되었다. 이를 위해 우선 국내외 건설산업 현황을 분석하였으며, 국내 건설업체들의 인식수준 및 니즈 파악을 위한 설문조사와 발전방안의 실효성 검토를 위한 전문가 심층면담을 순차적으로 진행하였다. 본 연구에서 도출한 국내 토목산업의 설계-시공 균형발전 방안은 1) 토목설계업 관리체계 효율화, 2) 토목설계업 관련 제도 선진화, 3) 고부가가치 우수인력 육성, 4) 엔지니어링조합 및 컨소시엄 구성, 5) 해외건설 협력기구 조직 및 운영으로 구분하여 제시되었다. In the recent global construction environment, EPC contracts that integrate planning, design, procurement, and construction have been widely used for industrial facilities. Similarly, as the importance of the engineering capability such as project management consulting(PMC) and front end engineering design(FEED) has been increased, design-build contracts also have been gradually adopted for infrastructure projects. Whereas, the Korean infrastructure industry has fragmentary features which mainly emphasize on the construction sector. Therefore, this study aims at developing strategies for enhancing the engineering capability and for balancing the competitiveness between engineering and construction sectors in the Korean infrastructure industry. To this end, the authors first carried out market analysis for both global and domestic market. Then, a structured survey and expert in-depth interviews were sequentially performed in order to reflect practitioners' perspectives and needs for institutional strategies. As a result, five strategies drawn from this study are as follows: 1) rearranging the government's roles for supporting the engineering sector; 2) establishing institutional bases being compatible with global standards; 3) fostering value-added workforce for undertaking complex tasks; 4) constructing an consortium between an engineering association and general contractors; and 5) building a nationwide collaborative networks for overseas market expansion.

임신한우의 혈액에 관한 연구 2 . 임신한우의 혈액학치에 관한 연구

이강욱,강희탁 ( Kang Wook Lee,Hi Tak Kang ) 한국축산학회 1976 한국축산학회지 Vol.18 No.5

경주마(競走馬) 혈청(血淸)의 총단백질(總蛋白質) 및 Ascorbic Acid 함량(含量)에 관하여

이강욱,용만중,Lee, Kang Wook,Yong, Mahn Joong 대한수의학회 1973 大韓獸醫學會誌 Vol.13 No.2

The concentrations of ascorbic acid and total protein in blood serum of 57 healthy race horses (17 males and 40 females) were observed. The results obtained were summarized as follows: 1) The mean value of ascorbic acid concentration was $0.44{\pm}0.16$ mg/100 ml (SD) ranging 0.20-0.92mg/100ml and sex difference was not significant. 2) The mean value of total protein concentration was $6.11{\pm}0.3$ g/170 ml(SD) ranging 5.6-7.0g/100 ml and their sex differences were significant(P<0.05); male $6.36{\pm}0.09$ (SE), female $6.13{\pm}0.06$ (SE). 3) Observation on the correlation between the total protein and ascorbic acid content (${\gamma}$=+0.3) and a liner repression equation (y=0.123x-0.31) were obtained.

Guinea pig의 갑상선(甲狀腺) 및 부갑상선척출(副甲狀腺剔出)이 각장기(各臟器) 및 조직(組織)에서의 방사성(放射性) 칼슘(⁴⁵Ca)의 흡수분포(吸收分布)에 미치는 영향(影響)

이강욱,이원창,정영채,Lee, Kang Wook,Lee, Won Chang,Chung, Young Chai 대한수의학회 1973 大韓獸醫學會誌 Vol.13 No.1

Seventy two guinea pigs were divided into 3 groups; 24 each of control, unilateral thyro-parathyroidectomized and bilateral thyro-parathyroidectomized groups. After the intraperifoneal in jection of $16{\mu}Ci$ of radioactive calcium ($^{45}Ca$) $^{45}Ca$ uptake of various organs and tissues were measured at the 8th, 16th, 24th and 36th hours, respectively, 18 animals were slaughtered (6 from each group) each time. The results obtained were as follows: 1. It was shown that each organ and tissue of treated animals had higher $^{45}Ca$ uptake than the control group, and the bilateralectomized group, higher than the unilateralectomized group, generally. However, there were some exceptions of the findings. The unilateralectomized group had higher $^{45}Ca$ uptake than the bilateralectomized group in case of adrenal gland and femur at the 8th hour; adrenal gland and blood at the 16th hour; spleen, pancreas, adrenal gland, femur and skeletal muscle at the 24th hour; and pancreas, femur and skeletal muscle at the 36th hour, respectively. 2. $^{45}Ca$ uptakes of each organ and tissue were also determind with respect to time. The results were varied with each treatment group, but generally the highest uptake was shown at the 8th hour and decreased gradually thereafter. The differences were not, however, statistically significant. 3. Femur had shown the highest $^{45}Ca$ uptake and the next were blood, pancreas, liver, skeletal muscle and spleen respectively, and the least were adrenal gland and liver, even though there were some variations in the results. 4. The differences in $^{45}Ca$ uptake of each organ and tissue for the treatment group were statstically significant as compared with the test of the control group, except the spleen and blood at the 8th hour; liver at the 16th hour; adrenal gland, spleen, pancreas at the 24th hour; and spleen at the 36th hour.