Long-Term (Postnatal Day 70) Outcome and Safety of Intratracheal Transplantation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Neonatal Hyperoxic Lung Injury

안소윤,장윤실,김수윤,성동경,김은선,So Yub Rime,유욱준,최수진,오원일,박원순 연세대학교의과대학 2013 Yonsei medical journal Vol.54 No.2

Purpose: This study was performed to evaluate the long-term effects and safety of intratracheal (IT) transplantation of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) in neonatal hyperoxic lung injury at postnatal day (P)70 in a rat model. Materials and Methods: Newborn Sprague Dawley rat pups were subjected to 14 days of hyperoxia (90% oxygen) within 10 hours after birth and allowed to recover at room air until sacrificed at P70. In the transplantation groups, hUCB-MSCs (5×105) were administered intratracheally at P5. At P70, various organs including the heart, lung, liver, and spleen were histologically examined, and the harvested lungs were assessed for morphometric analyses of alveolarization. ED-1, von Willebrand factor, and human-specific nuclear mitotic apparatus protein (NuMA) staining in the lungs and the hematologic profile of blood were evaluated. Results: Impaired alveolar and vascular growth, which evidenced by an increased mean linear intercept and decreased amount of von Willebrand factor, respectively, and the hyperoxia-induced inflammatory responses, as evidenced by inflammatory foci and ED-1 positive alveolar macrophages, were attenuated in the P70 rat lungs by IT transplantation of hUCB-MSCs. Although rare, donor cells with human specific NuMA staining were persistently present in the P70 rat lungs. There were no gross or microscopic abnormal findings in the heart, liver, or spleen, related to the MSCs transplantation. Conclusion: The protective and beneficial effects of IT transplantation of hUCB-MSCs in neonatal hyperoxic lung injuries were sustained for a prolonged recovery period without any long-term adverse effects up to P70.

물리치료사와 물리치료과 학생의 HIV/AIDS에 대한 지식과 태도

안소윤,김병조,김수민,김재호,김종순 대한물리치료학회 2004 대한물리치료학회지 Vol.16 No.4

Mesenchymal stem cells transplantation for neuroprotection in preterm infants with severe intraventricular hemorrhage

안소윤,장윤실,박원순 대한소아청소년과학회 2014 Clinical and Experimental Pediatrics (CEP) Vol.57 No.6

Severe intraventricular hemorrhaging (IVH) in premature infants and subsequent posthemorrhagichydrocephalus (PHH) causes significant mortality and life-long neurological complications, includingseizures, cerebral palsy, and developmental retardation. However, there are currently no effectivetherapies for neonatal IVH. The pathogenesis of PHH has been mainly explained by inflammationwithin the subarachnoid spaces due to the hemolysis of extravasated blood after IVH. Obliterativearachnoiditis, induced by inflammatory responses, impairs cerebrospinal fluid (CSF) resorption andsubsequently leads to the development of PHH with ensuing brain damage. Increasing evidence hasdemonstrated potent immunomodulating abilities of mesenchymal stem cells (MSCs) in various braininjury models. Recent reports of MSC transplantation in an IVH model of newborn rats demonstratedthat intraventricular transplantation of MSCs downregulated the inflammatory cytokines in CSF andattenuated progressive PHH. In addition, MSC transplantation mitigated the brain damages that ensueafter IVH and PHH, including reactive gliosis, cell death, delayed myelination, and impaired behavioralfunctions. These findings suggest that MSCs are promising therapeutic agents for neuroprotection inpreterm infants with severe IVH.

Mulligan 의 도수치료 개념

안소윤,변만호 대한물리치료학회 1998 대한물리치료학회지 Vol.10 No.1

This study was introduce to Manual Therapy of Mulligan. The use of sustained natural apophyseal glides (SNAGS) and mobilisation with movement(MWM) for spine and peripheral joints has been developed by Mulligan. A mobilisation is applied parallel or right angles to restricted joint movement. If the applied mobilisation achieves immediate improvement in the functional movement and abolishes the pain the treatment involves sustaining the mobilisation while the patient performs the active movement repetitively. On reassessment of the joint function the movement should remain improved without the mobilisation. Theories as to why these techniques provide rapid improvement in pain-free range are proposed, and general principle of examination and treatment are outlined.

신생아 지속성 폐동맥 고혈압증에서 흡입 일산화질소 치료 임상 경과와 부작용

안소윤 대한신생아학회 2015 Neonatal medicine Vol.22 No.2

Inhaled nitric oxide (iNO) is a potent and selective pulmonary vasodilator agent that improves arterial oxygenation and subsequent clinical outcomes for newborn infants with persistent pulmonary hypertension of the newborn (PPHN). Along with beneficial pharmacological properties, iNO also shows toxicological effects. Although the side effects of iNO have not been fully understood, these need to be thoroughly considered and monitored for the safe and effective clinical use of iNO. This article presents a review of the side effects of iNO and short-term and long-term clinical prognosis in newborn infants ≥34 weeks’ gestation with PPHN.

부산지역 제조업 근로자들의 요통과 작업환경과의 관계

안소윤,신해림,손성근,김용완 인제대학교 1991 仁濟醫學 Vol.12 No.1

산업장 근로자들의 요통과 작업환경과의 관계를 조사한 결과 근로자들의 요통경험율은 58.4%였으며 현재 직업과 관련된 요통경험율은 요통근로자들의 54.1%였다. The low back pain (LBP) is not a new problem and it has been a major problem in industry. This study has been made to determine self-reported LBP rate and to know the relation between work environment and work-related LBP by questionnaire survey. The study subjects are 269 workers in the manufacturing industries in Pusan and the study period is from December 1 to 30 in 1989. The results are summerized as follows; 1.The mean age of the workers are 35.11±10.3 years and 64.4% of workers are 20-40 years. 2.Self-reported LBP rate is 58.4% among the 269 workers. 3.54.l% of LBP workers complaint work-related back pain(WBP). 4.There were significant differences between LBP workers and WBP workers about the fitness of the working chair (p<0.01), the fitness of the working table (p<0.05).

Pseudohypoaldosteronism in a premature neonate with severe polyhydramnios in utero

안소윤,신손문,김경아,이연경,고선영 대한소아청소년과학회 2009 Clinical and Experimental Pediatrics (CEP) Vol.52 No.3

We report a case of a premature newborn baby who presented with hyponatremia, hyperkalemia, and metabolic acidosis accompanied by severe polyhydramnios in utero. The baby was diagnosed with pseudohypoaldosteronism on the basis of normal 17-hydroxyprogesterone levels, elevated aldosterone, and clinical symptoms. His serum electrolyte levels were corrected with sodium chloride supplementation. Sodium supplementation was reduced gradually and discontinued at 5 months of age. At 5 months, the child was able to maintain normal serum electrolyte levels without oral sodium chloride supplementation, and showed normal physical and neurological development. This case illustrates that pseudohypoaldosteronism must be considered if a newborn infant with an antenatal history of severe polyhydramnios shows excessive salt loss with normal levels of 17-hydroxyprogesterone.

한국 신생아네트워크

안소윤 한국모자보건학회 2017 한국모자보건학회 학술대회 연제집 Vol.2017 No.1

물리치료학 교육목표와 표준 교과과정안 개발

안소윤,안창식,이완희,박래준,Ahn, So-Youn,Ahn, Chang-Sik,Lee, Wan-Hee,Park, Rea-Jun 대한물리치료과학회 2007 대한물리치료과학회지 Vol.14 No.1

We discuss why many current physical therapy entry-level programs are not designed to educate the type of physical therapy practitioners needed for the 21st century. We face a situation that we have to promote the profession's role in the prevention, diagnosis, and treatment of movement dysfunctions and the enhancement of the physical health and functional abilities of members of the public. The way how to train professional physical therapists became crucial. The purpose of study was to enhance the educational goals of physical therapy and the physical therapy curricula in Korea. In this study, we compared physical therapy training curricula recommended by WCPT with physical therapy training curricula in universities in other countries by dividing physical therapy curricula in universities and colleges into physical therapy students' electives, major required courses, labs and clinical practices, and counting the proportion of each category in the total credit hours. We discuss differences and similarities between curriculum in a university in Korea and curriculum in a university in the United States. We discussed possibly problematic portions of current physical therapy training curricula in korean universities. Finally, we statistically analyzed the regulations of WCPT and Health and Welfare Ministry in Japan, the Physical therapy curriculum of Creighton Entry-level DPT Program in the U.S. and the Inje University in Korea. The progressing direction of curricula in Korea had been researched. The future direction that korean physical therapy is to use an united curriculum that includes basic requirements of WCPT for all universities and colleges in Korea, rather than using each university's own modified version. The results of study can be helpful for developing a basic level of integrated curricula in universities and colleges in Korea.

물리치료사의 근무실태와 직무만족 및 직장애착

안소윤,김원중,허영배,Ahn Soyoun,Kim Won-Joong,Huh Young-Bae 대한물리치료학회 2002 대한물리치료학회지 Vol.14 No.4

The main objective of this paper is to examine the working conditions of physical therapists and to investigate the level of job satisfaction and organizational commitment under different working conditions. A survey was conducted through structured questionnaire for the physical therapists working in Busan area, and data from 175 therapists were utilized in the final analysis. Survey items included general characteristics of the therapists(sex, age, education, marital status, religion, income, career years, etc.), their working conditions(kinds of organizations they work in, location of PT room, weekly working hours, average number of patients, number of co-workers, equipments/facilities, etc.), and organizational effectiveness measured in terms of job satisfaction and organizational commitment. Major results are as follows: 1) As for the general characteristics of the surveyed physical therapists, male(51.4$\%$) slightly exceeded female(48.6$\%$) and majority(more than 90$\%$) was 20-39 in age. Also, there were more single(57.7$\%$) than married(42.3$\%$), and 54.9$\%$ of the respondents had religion while 45.1$\%$ did not. In terms of the ranks in their organizations, only a few of them(6.9$\%$) were managers, and monthly salary mostly ranged from $\￦$1,000,000 to $\￦$2,500,000. More than half of them worked in various kinds of hospitals, with the remaining in health centers or social welfare institutions. 2) In regard to the working conditions of the respondents, 19.4$\%$ of PT rooms were located in basement areas while the remaining 80.6$\%$ in first to third floors. 34.3$\%$ of them treated 15 patients or less per day, whereas 25.1$\%$ treated 31 patients or more. Also, 52$\%$ recorded physical therapy charts periodically. As for the equipments and facilities, 25.1$\%$ felt 'sufficient' and 40$\%$'insufficient.' 3) The respondents provided various kinds of suggestions for the improvement of their working conditions, where the most important were 'salary raise' and 'reduction of working hours.' In addition, their requests to the PT Association included 'permission of opening of independent practice,' 'permission of legal specialty,' and 'vitalization of the Association.' 4) The comparative analysis of job satisfaction and organizational commitment among different characteristics of the respondents revealed that there was no significant difference between male and female, but in terms of age, the group of 40 or older was highest in both aspects. Besides, therapists who had 15 or more years of hospital career reported higher job satisfaction and organizational commitment than the others. It was also found that the level of organizational commitment increased as the rank of the respondents got higher. There were no significant differences in terms of marital status and religion. 5) Comparison with regard to working conditions showed that therapists employed in university hospitals, health centers and social welfare institutions felt higher job satisfaction and organizational commitment than those in community hospitals and clinics. Also, respondents who worked in basement areas had significantly lower job satisfaction than otherwise. The length of weekly working hours did not really affect the level of job satisfaction, but it was important to maintain 'regular working hours' for the hospitals to improve the employees' organizational commitment. As for the number of patients per day, 'medium level' (21-25 patients) had highest scores in both aspects. And, finally, sufficiency of equipments and facilities was found to be important for the improvement of organizational commitment.