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우리나라에 발생하는 잿빛곰팡이병균 Botrytis cinerea의 분자계통학적 유연관계
백창기,이승열,정희영 한국균학회 2014 韓國菌學會誌 Vol.42 No.2
Several isolates were collected from apple, pepper, strawberry, cucumber and tomato having typical gray moldsymptoms. All the isolates were identified as Botrytis cinerea by using morphological characteristics and PCR-RFLP method. It wasdifficult to analyze the phylogenetic relationship of these isolates by using ITS region, HSP60 and G3PDH because these geneswere highly homologous in their nucleotide in inter-species of B. cinerea and intra-species of genus Botrytis. However, phylogeneticanalysis using combined sequences (RPB2, HSP60 and G3PDH genes) clearly showed that all isolate of B. cinerea were differentfrom Botrytis spp. Furthermore, it was also confirmed that strawberry isolate was distantly related to apple, pepper, cucumber andtomato isolates that were closely related to each other in nucleotide level. 잿빛곰팡이병의 전형적인 병징을 나타내는 병든 사과, 고추, 딸기, 오이, 토마토에서 곰팡이를 분리하고, 그들의 배양학적 특성과, 형태적 특성 및 PCR-RFLP을 통해 이 병원성 곰팡이를 모두 Botrytis cinerea로 동정하였다. 또한, 배양학적 특징에 따라 사과, 고추, 오이에서 분리한 잿빛곰팡이병균의 표현형은 균핵형이며, 딸기와 토마토에서 분리한잿빛곰팡이병균은 균사형이었다. 각각의 잿빛곰팡이병균의 ITS 영역 염기서열을 포함한4종의 유전자(RPB2, HSP60, G3PDH)의 염기서열을 결정하고 분자계통학적 유연관계를 분석하였다. RPB2 유전자염기서열을 제외한 ITS 영역, HSP60유전자 및 G3PDH 유전자의 염기서열은 Botrytis cinerea 종 내 뿐만 아니라Botrytis 속 종간에도 매우 높은 상동성을 나타내어 계통학적 유연관계 분석이 어려웠다. 하지만, 3종의 유전자(RPB2, HSP60, G3PDH)를 결합한 유전자 염기서열을 이용한 분자계통수 작성 결과, 본 연구에서 분리한 잿빛곰팡이병균은Botrytis 속의 다른 종들과 구별되며, 사과, 고추, 오이, 토마토의 분리주는 아주 높은 근연관계에 있고, 딸기잿빛곰팡이병균은 다른 분리주와 달리 종내 다른 lineage를 형성하였다.
백창기,한유경,Walftor Bin Dumin,박종한,한지원,배영석 충남대학교 농업과학연구소 2022 Korean Journal of Agricultural Science Vol.49 No.1
Leaf blight disease caused by Stemphylium species is an important disease in Allium crops, specifically onion, garlic and welsh onion. In 2018, leaf blight symptoms were severe and damaged onion and garlic in Jeonnam province in Korea. In addition, small purple spots on garlic burbs were observed in a post-harvest storage warehouse. Several Stemphylium isolates were isolated from diseased leaves from the field and from garlic bulb samples and were analyzed in terms of homology and the phylogenetic relationship based on the internal transcribed spacer region and calmodulin gene sequence. The results showed that among three Stemphylium species identified, S. vesicarium is most prevalent on onion and garlic. S. eturmiunum was for the first time identified as pathogenic to onion and garlic, whereas S. solani was found in welsh onion crops. Although these isolates grew well at the optimum temperature at 20 - 25℃, they could also grow at low temperatures of 10 - 15℃. A pathogenicity test was conducted using S. vesicarium and S. eturmiunum on onion and garlic respectively. These results showed that two Stemphylium species were highly virulent with cross pathogenicity in onion and garlic. The results of this study can support the biological characterization of Stemphylium species in Korea. Moreover, further research will need to develop fungicide application strategies for onion and garlic crops.
백창기,박미정,박건석,양창열,박종한 한국미생물학회 2020 미생물학회지 Vol.56 No.1
Xanthomonas arboricola pv. pruni is a causal agent of bacteria that caused “shot hole disease” on peach and stone fruits in South Korea. The complete genome of X. arboricola pv. pruni strain KACC21687 contains 5,141,998 bp with G + C contents of 65.38%, including 4,161 protein-coding genes in a circular chromosome. The type I, II, III, IV, and VI effector proteins were encoded 55 genes. These gene clusters may play an important role in recognizing host cells and virulence potential.
백창기,이승열,이부자,예미지,김상목,강인규,차재순,정희영 한국식물병리학회 2015 Plant Pathology Journal Vol.31 No.3
In this study, we developed a species-specific PCR assay for rapid and accurate detection of three Xanthomonas species, X. axonopodis pv. poinsettiicola (XAP), X. hyacinthi (XH) and X. campestris pv. zantedeschiae (XCZ), based on their draft genome sequences. XAP, XH and XCZ genomes consist of single chromosomes that contain 5,221, 4,395 and 7,986 protein coding genes, respectively. Species-specific primers were designed from variable regions of the draft genome sequence data and assessed by a PCR-based detection method. These primers were also tested for specificity against 17 allied Xanthomonas species as well as against the host DNA and the microbial community of the host surface. Three primer sets were found to be very specific and no amplification product was obtained with the host DNA and the microbial community of the host surface. In addition, a detection limit of 1 pg/μl per PCR reaction was detected when these primer sets were used to amplify corresponding bacterial DNAs. Therefore, these primer sets and the developed species-specific PCR assay represent a valuable, sensitive, and rapid diagnostic tool that can be used to detect three specific pathogens at early stages of infection and may help control diseases.
양파 마른썩음병 원인균 동정과 약제배지를 균사생장 억제효과 검정
백창기,이종태,Walftor Bin Dumin,한유경,박종한,배영석 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.11
양파는 우리나라에서 재배하는 겨울철 작물 중 하나로 그 재배적 가치가 매우 높다. 양파는 흑색썩음균핵병, 마른썩음병(시들음병), 무름병 등이 발생하면 수확량이 감소하게 된다. 본 연구에서는 양파 마른썩음병의 원인균을 분리하여 병원성 검정, 종 동정을 실시하였다. 그 결과, Fusarium oxysporum과 F. commue균을 분리하였고, 이 중 F. oxysporum이 병원성이 높은 것으로 조사되었다. 양파에서 분리한 마른썩음병균의 균사 생장억제효과를 검정하기 위하여 양파에 사용되는 6종의 살균제로 약제 희석배지를 제조하고 이를 이용하여 균사생장 억제효과를 검정하였다. 그 결과, 프로클로라즈망가니즈 등 2종 이상의 살균제에서 마른썩음병균의 균사생장을 효과적으로 억제하는 것으로 확인되었다. 현재, 양파 마른썩음병 방제용 살균제가 전무한 상태이므로, 향후 포트시험 등을 통한 양파 마른썩음병 방제효과를 검정하고, 포장시험 등을 통해서 양파 마른썩음병 방제용 살균제를 등록이 필요할 것으로 판단된다.