메티실린 내성 황색 포도상 구균에서 mecA, femA 유전자의 임상적 의의

박정은,김택선,박수성,김은령,김일수,안일영,김영진,김재종,강성옥,박한오,Park, Jung-Eun,Kim, Taek-Sun,Park, Su-Sung,Kim, Eun-Ryoung,Kim, Il-Su,Ann, Il-Young,Kim, Young-Jin,Kim, Jae-Jong,Kang, Sung-Ok,Park, Han-Ho 대한소아감염학회 1996 Pediatric Infection and Vaccine Vol.3 No.2

Purpose : In the treatment of MRSA infection, rapid detection of MRSA is extremely important. The mecA gene codes the new drug resistant polypeptides called PBP2' which mediates the clinically relevant resistance to all beta-lactam antibiotics. The identical mecA gene has been found in coagulase-negative staphylococcus with the methicillin-resistant phenotype. On the other hand, the femA gene was absent from coagulase negative staphylococcus strains with the methicillin resistant phenotype. This study is aimed at early detection and definite diagnosis of MRSA. Methods : A total of 24 MRSA strains were studied. All strains were tested for antimicrobial susceptibility and purified DNA. We amplified both mecA and femA genes by PCR in 24 strains. Results : In MRSA all the 16 strains (100%) carried femA gene and 11 strains (68.7%) carried mecA gene. In contrast, in methicillin sensitive staphylococcus all the 8 strains (100%) carried femA and only 3 strains (37.5%) were detected mecA. Conclusions : As results, there are difference in the phenotype and genotype of methicillin resistance by PCR of mecA and femA. Such disparities between methicillin resistance and the presence of mecA gene suggest the presence of control gene of the mecA.

물안보 확보를 위한 탄력적 하천유량관리 기술 개발

박정은,최현구,김정곤,Park, Jeong-Eun,Choe, Hyeon-Gu,Kim, Jeong-Gon 한국수자원학회 2014 물과 미래(한국수자원학회지) Vol.47 No.2

인공지능 스피커의 세대별 온라인 리뷰 분석을 통한 사용자 경험 요인 탐색

박정은,양동욱,김하영,Park, Jeongeun,Yang, Dong-Uk,Kim, Ha-Young 한국융합학회 2021 한국융합학회논문지 Vol.12 No.7

인공지능 스피커 시장은 꾸준히 성장하고 있지만, 실제 스피커 사용자들의 만족도는 42%에 그치고 있다. 따라서, 본 연구에서는 인공지능 스피커의 세대별 토픽 변화와 감성 변화를 통해 사용자 경험을 저해하는 요소는 무엇인지 분석해 보고자 한다. 이를 위해 아마존 에코 닷 3세대와 4세대 모델에 대한 리뷰를 수집하였다. 토픽모델링 분석 기법을 사용하여 세대별로 리뷰를 이루는 주제 및 주제의 변화를 찾아내고, 딥러닝 기반 감성 분석을 통해 토픽에 대한 사용자 감성이 세대에 따라 어떻게 변화되었는지 살펴보았다. 토픽모델링 결과, 세대별로 5개의 토픽이 도출되었다. 3세대의 경우 스피커의 일반적 속성을 나타내는 토픽은 제품에 긍정적 반응 요인으로 작용했고, 사용자 편의 기능은 부정적 반응 요인으로 작용했다. 반대로 4세대에서는 일반적 속성은 부정적으로, 사용자 편의 기능은 긍정적으로 도출되었다. 이와 같은 분석은 방법론 측면에서 어휘적 특징뿐 아니라 문장 전체의 문맥적 특징이 고려된 분석결과를 제시할 수 있다는 것에 그 의의가 있다. The AI speaker market is growing steadily. However, the satisfaction of actual users is only 42%. Therefore, in this paper, we collected reviews on Amazon Echo Dot 3rd and 4th generation models to analyze what hinders the user experience through the topic changes and emotional changes of each generation of AI speakers. By using topic modeling analysis techniques, we found changes in topics and topics that make up reviews for each generation, and examined how user sentiment on topics changed according to generation through deep learning-based sentiment analysis. As a result of topic modeling, five topics were derived for each generation. In the case of the 3rd generation, the topic representing general features of the speaker acted as a positive factor for the product, while user convenience features acted as negative factor. Conversely, in the 4^th generation, general features were negatively, and convenience features were positively derived. This analysis is significant in that it can present analysis results that take into account not only lexical features but also contextual features of the entire sentence in terms of methodology.

탄력성과 탄력적 물관리, 그리고 물안보 -1. 탄력성의 개념과 탄력적 물관리의 등장

박정은,김정곤,김현식,고덕구,Park, Jeong-Eun,Kim, Jeong-Gon,Kim, Hyeon-Sik,Go, Deok-Gu 한국수자원학회 2013 물과 미래(한국수자원학회지) Vol.46 No.5

근접치료용 방사성 동위원소의 선량분포 확인을 위한 디지털 반도체 센서의 제작 및 평가

박정은,김교태,최원훈,이호,조삼주,안소현,김진영,송용근,김금배,허현도,박성광,Park, Jeong-Eun,Kim, Kyo-Tae,Choi, Won-Hoon,Lee, Ho,Cho, Sam-Joo,Ahn, So-Hyun,Kim, Jin-Young,Song, Yong-Keun,Kim, Keum-bae,Huh, Hyun-Do,Park, Sung-Kwang 한국의학물리학회 2015 의학물리 Vol.26 No.4

방사선 치료분야 중 근접치료는 방사성 동위원소를 체내에 직접 삽입하여 병변 세포를 사멸시키는 치료법으로써, 주로 고선량률 치료가 시행되고 있다. 현재 치료계획과 실제 선량 방출범위의 일치성 여부는 Film/Screen 시스템을 통해 확인하고, 확인된 선량분포에 따라 방사선 치료를 시행하고 있다. 선량 분포 확인 시 F/S 시스템을 이용할 경우, Film 현상조건에 따른 신호 왜곡과 반음영에 의한 저분해능으로 인하여 치료계획과의 선량 분포 일치성을 정량적으로 파악하기 힘든 단점이 있다. 본 연구에서는 방사선 근접치료 시 치료계획과 동일한 선량 분포 여부를 확인하는 디지털 검출시스템의 기초 연구를 진행하고자, PIB법을 이용한 $HgI_2$ 반도체 검출센서를 제작하였다. 또한 이를 근접치료선원을 이용해 평가함으로써, QA 시스템으로 이용 가능성을 검증하고자 하였다. 근접치료 범위의 확인을 위하여 SDD의 변화에 대한 신호 수집량을 평가한 결과, 치료 범위 이상의 거리에서는 산란선으로 추정되는 낮은 신호만이 측정되었으므로 치료 계획시와 동일한 치료 범위를 정량적으로 확인할 수 있었다. 또한 동일한 ${\gamma}$-선 조사 조건에 대한 재현성 평가 결과, 변동계수 1.5% 이내인 것을 확인하였다. 이와 같은 결과를 바탕으로, 본 연구에서 제작한 센서는 방사선 근접치료 QA 시스템으로 적용 가능할 것이라 사료된다. In radiation therapy fields, a brachytherapy is a treatment that kills lesion of cells by inserting a radioisotope that keeps emitting radiation into the body. We currently verify the consistency of radiation treatment plan and dose distribution through film/screen system (F/S system), provide therapy after checking dose. When we check dose distribution, F/S systems have radiation signal distortion because there is low resolution by penumbra depending on the condition of film developed. In this study, We fabricated a $HgI_2$ Semiconductor radiation sensor for base study in order that we verify the real dose distribution weather it's same as plans or not in brachytherapy. Also, we attempt to evaluate the feasibility of QA system by utilizing and evaluating the sensor to brachytherapy source. As shown in the result of detected signal with various source-to-detector distance (SDD), we quantitatively verified the real range of treatment which is also equivalent to treatment plans because only the low signal estimated as scatters was measured beyond the range of treatment. And the result of experiment that we access reproducibility on the same condition of ${\gamma}$-ray, we have made sure that the CV (coefficient of variation) is within 1.5 percent so we consider that the $HgI_2$ sensor is available at QA of brachytherapy based on the result.

아연-공기 전지용 전해질의 Gelling Agent 분자량에 따른 자가 방전 억제 효과

박정은,조용남,Park, Jeong Eun,Jo, Yong Nam 한국재료학회 2019 한국재료학회지 Vol.29 No.12

A zinc-air battery is one of most promising advanced batteries due to its high specific energy density, low cost, and environmental friendliness. However, zinc anodes in zinc-air batteries lead to several issues including self-discharge, corrosion reaction, and hydrogen evolution reaction (HER). In this paper, viscosity of electrolyte has been controlled to suppress the corrosion reaction, HER, and self-discharge behavior. Various viscosity average molecular weights of poly(acrylic acid) (PAA) are adopted to prepare the electrolyte. The evaporation of electrolytes is proportional to the increase in molecular weight. In addition, enhanced self-discharge behavior is obtained when the gelling agent with high molecular weight is used. In addition, the zinc-air cell assembled with lower viscosity average molecular weight of PAA (M_v ~ 450,000) delivers 510.85 mAh/g and 489.30 mAh/g of discharge capacity without storage and with 6 hr storage, respectively. Also, highest capacity retention (95.78 %) is obtained among studied materials.

사회변화와 간호원의 복지전략 개발을 위한 연찬회 보고

박정은,Park J E 대한간호협회 1988 대한간호 Vol.26 No.5

HJ01이 OP9세포에서의 지방 분화와 P-407로 유발한 고지혈증 흰쥐에 미치는 영향

박정은,한상용,최은식,정명수,김윤경,Park, Jung-Eun,Han, Sang-Yong,Choi, Eun-Sik,Chong, Myong-Soo,Kim, Yun-Kyung 대한한의학방제학회 2013 大韓韓醫學方劑學會誌 Vol.21 No.1

Objectives : This study was designed to investigate the effect of a herbal preparation HJ01 consisting of Salicornia herbacea, Citri Reticulatae Pericarpium, Crataegi Fructus and Glycyrrhizae Radix on adipocyte differentiation in OP9 cells and on poloxamer 407(P-407)-induced hyperlipidemia in mice. Methods : 1. MTT assay was used to evaluate the potential cytotoxicity of Salicornia herbacea, Citri Reticulatae Pericarpium, Crataegi Fructus, Glycyrrhizae Radix and HJ01, respectively. 2. Bone-marrow derived OP9 cells were treated with HJ01, and the alterations in fat storage in the cells were determined by the Oil red O assay. 3. The protein level of CAAAT/enhancer binding protein alpha($C/EBP{\alpha}$), as a adipocyte differentiation marker, was examined using western blot analysis in differentiated OP6 cells. 4. Adult male C57BL6 mice received intraperitoneal injections of P407 to induce hyperlipidemia, simultaneously, were treated with HJ01 for 4 weeks. Then the cholesterol (TC), triglyceride (TG) and high-density lipoprotein-cholesterol (HDL-c) levels in sera and liver tissues were measured. Results : 1. The MTT assay exhibited that Salicornia herbacea, Citri Reticulatae Pericarpium, Crataegi Fructus, Glycyrrhizae Radix and HJ01 showed no significant cytotoxicity in tested dosages. 2. Ten days' treatment with HJ01 markedly inhibited the increases in fat storage in differentiated OP6 cells. 3. Four weeks' treatment with HJ01 down-regulated the protein level of CAAAT/enhancer binding protein alpha($C/EBP{\alpha}$) but up-regulated the levels of adiponectin in differentiated OP9 cells. 5. HJ01 inhibited the accumulation of TC and TG in liver tissues and increased serum levels of TC in hyperlipidemic mice. Conclusions : These results suggest that HJ01 can in vitro inhibit adipocyte differentiation and fat storage in OP6 cells, in vivo improve the hyperlipidemia induced by P-407 in mice, which may be mediated by promoting glucose uptake and improving a lipid metabolite profile.

물안보와 지속가능한 개발 : 물안보 도전과제와 이해당사자간 협력

박정은,이을래,임광섭,채효석,Park, Jeong-Eun,Lee, Eul-Rae,Im, Gwang-Seop,Chae, Hyo-Seok 한국수자원학회 2015 물과 미래(한국수자원학회지) Vol.48 No.7

지류 중소하천의 물관리 탄력성 평가지수 개발 및 평가

박정은,이을래,임광섭,Park, Jung Eun,Lee, Eul Rae,Lim, Kwang Suop 한국수자원학회 2016 한국수자원학회논문집 Vol.49 No.2

지류 중소하천의 미래 물관리 정책방향을 수립하기 위한 자료로, 물관리 탄력성 지수를 개발하고 이를 117개 중권역에 적용하였다. 물관리 탄력성 지수는 취약성, 견고성, 다양성의 부지수로 구성하였으며, 각 부지수는 다시 이수, 치수, 하천환경 분야의 지표들로 총 31개의 지표항목으로 구성하였다. 중권역은 다목적댐 하류 본류유역(범주 1), 용수공급 및 유량조절이 불가능한 지류(범주 2)와 가능한 지류(범주 3)로 분류하여 적용한 결과, 지류 중소하천에서의 물관리 탄력성, 특히 이수 및 치수분야의 다양성이 본류에 비해 낮은 것으로 나타났다. 또한 취약성과 견고성에 있어서는 본류와 지류가 큰 차이를 보이지 않았으나 다양성에서 지류가 낮은 값을 나타내었다. 따라서 지류 중소하천의 다양성을 높이는 방향으로 물관리 정책의 수립이 필요하다. 본 연구에서 개발된 물관리 탄력성 지수는 내외부 충격에 대한 안정성을 종합적으로 제시하여 수자원 정책 및 계획의 우선지역 선정 등에 활용할 수 있을 것이다. Water Management Resilience Index (WMRI) was developed as a policy measure of adaptability to withstand water stresses and to set up water management strategies mainly in mid-small scale tributaries, and then evaluated on 117 sub-basins in South Korea. The index consists of 3 sub-indices such as vulnerability, robustness and redundancy sub-indices, each including indicators of 3 sectors: water use, flood mitigation, and river environment. Total number of indicators selected for the index was 31. Taking into account the stream order and control capability of river flow discharge, sub-basins were categorized into 3: 1 for mainstreams of lower large dams, 2 and 3 for tributaries, respectively without and with flow discharge regulation. As a result of the evaluation, resilience index scores in Category 2 and 3 are much lower than that of Category 1, especially with very poor score of redundancy. Although there was no significant difference between mainstream and tributaries in vulnerability and robustness sub-indices, results of redundancy sub-index in tributaries were lower than those in mainstream. Thus, it is conceived that the variety of water management schemes should be considered to improve their resilience in the face of future uncertainty. Addressing comprehensive stability of river basin against internal and external impacts, WMRI in this study can also be used for the prioritization of water management plans.