Anti-inflammatory Effects of Pinitol (3-O-methyl-chiro-inositol) in Rats

김종춘,이상민,박소양,신동호,김성호,신용철 한국실험동물학회 2006 Laboratory Animal Research Vol.22 No.1

This study evaluated the anti-inflammatory effect of a natural compound, pinitol (3-O-methyl-chiro-inositol) against acute or subacute inflammation in rats. The single oral administration of pinitol at 5 and 20 ㎎/㎏ showed a significant inhibition of paw edema in a dose-dependent manner. Although no statistically significant difference was noted, the 7-day administration of pinitol at 5 and 20 ㎎/㎏ also showed a tendency to decrease the weights of granuloma formed by cotten pellet implantation. It was concluded that pinitol has an anti-inflammatory effect on acute and subacute inflammation. These results suggest that a pinitol can be developed as an anti-inflammatory drug or a functional food to prevent or attenuate various types of inflammation-related diseases.

Reader Preferences in Teacher-guided and Self-directed Extensive Reading Program

김종춘 사단법인 한국융합기술연구학회 2023 아시아태평양융합연구교류논문지 Vol.9 No.11

Extensive Reading (ER) is a pedagogical approach in second language (L2) education that has a strong foundation and is highly effective in fostering a passion for reading and enhancing reading proficiency. This study aimed to investigate the diverse landscape of ER, revealing the extensive range of genres, topics, and book selections it contains. The study examined a group of 83 nursing students in Seoul, who were divided into two groups: one receiving teacher guidance and the other engaging in self-directed learning. The program lasted for a duration of 15 weeks and focused on extensive reading training. The results indicated a strong preference for non-fiction literature, particularly in the field of Science, which aligns with the professional interests of the nursing students who took part in the study. The group that was supervised by the teacher demonstrated a wider range of subject study, particularly in the areas of Science, Social Studies, and History, highlighting the significance of having organized direction. On the other hand, the self-directed group exhibited a more equitable distribution of reading materials, encompassing both fiction and non-fiction genres. The observation may potentially indicate a correlation with their personal motivations and educational paths. Notable correlations were observed among specific subjects, suggesting a strong inclination towards interdisciplinary pursuits and the possibility of integrating disparate areas of knowledge. The books that are most frequently read tend to be focused on the subject of Science, while also maintaining a balanced reading level. This suggests that students have a preference for materials that are neither excessively simplistic nor excessively hard. Despite being somewhat overshadowed by factual content, narratives continue to maintain their attractiveness, therefore underlining their crucial position in the landscape of extensive reading settings. Furthermore, the study provides valuable perspectives on the reading habits of second language (L2) learners, emphasizing the ongoing popularity of narratives, the need of multidisciplinary education, and the delicate equilibrium between self-direction and instructional support. The aforementioned discoveries have the potential to impact instructional approaches, leading to a more effective integration of student needs and interests. Additionally, they provide a foundation for future investigations into the underlying motivations driving these preferences for reading materials.

Evalution of Anti-diarrheal Activity of Bamboo Charcoal in Rats

김종춘,양영수,Bo-Kyoung Park,김성호,신동호,박승춘 한국실험동물학회 2006 Laboratory Animal Research Vol.22 No.1

경유 중 황 및 방향족화합물 조성에 따른 배출가스 특성연구 : 미량대기오염물질 중심으로

김종춘,서충열,임윤성,임철수,임재현,이종태,윤창완,이상일,김홍규,김상규,황춘식 한국대기환경학회 2008 한국대기환경학회 학술대회논문집 Vol.- No.-

Evaluation of the testicular toxicity caused by 2-bromopropane in rats

김종춘,이현숙,윤효인,정문구,Kim, Jong-choon,Lee, Hyun-sook,Yun, Hyo-in,Chung, Moon-koo The Korean Society of Veterinary Science 2000 大韓獸醫學會誌 Vol.40 No.2

최근 2-bromopropane(2-BP)이 사람과 실험동물에서 정소독성을 유발한다고 보고된 바 있다. 그러나 수컷 생식기계에 있어서 2-BP의 지연효과에 대해서는 세부적으로 조사된 바가 없다. 본 연구는 Sprague-Dawley 랫드에서 2-BP의 정소독성과 정자발생의 회복을 조사하기 위하여 수행하였다. 5주령의 수컷 랫드에게 2-BP를 1,000mg/kg 용량으로 4주간 반복투여하였고, 투여시작후 1, 2, 3, 4 및 12주째에 부검하였다. 정소독성의 평가는 병리조직학적인 질적평가와 생식기관 중량, 정자두부수 및 재생지수 등의 양적평가로 수행하였다. 시험결과 2-BP를 투여한 랫드에서는 체중과 정소 및 정소상체 중량이 대조군에 비해 시간의존적인 방식으로 억제 또는 감소하였다. 병리조직검사에서는 투여 1주째에 stage I~IV에서 정조세포와 stage VII~IX에서 세사전기 및 세사기의 정모세포가 현저하게 소실되었다. 정조세포는 투여 2주째에 모든 stage에서 광범위하게 소실되었으며, 정자발생주기가 진행됨에 따라 2, 3 및 4주째에는 접합기 정모세포, 비후기 정모세포 및 원형 정자세포가 전구세포의 결손에 의해 점진적으로 소실되었다. 지지세포의 기능적 이상을 암시하는 지지세포의 공포화와 정자세포 저류는 상기한 모든 시기에서 관찰되었다. 8주 회복후인 12주째에는 대부분의 곡세정관이 심하게 위축되어 지지세포만 관찰되었으며, 간질조직에서는 간질세포의 과형성이 인정되었다. 또한 2-BP에 의해 유발된 정소의 손상이 비가역적임을 암시해주는 정자두부와 재생지수의 현저한 감소가 관찰되었다. 상기결과는 랫드의 2-BP를 1,000mg/kg의 용량으로 4주간 반복투여하면 정조세포의 결손에 의해 점진적으로 생식세포가 감소하고 이로 인하여 장기적인 정소위축이 유발된다는 것을 암시해준다. It has been recently reported that 2-bromopropane (2-BP) induces male reproductive toxicity in both human and experimental animals. However, delayed effects of 2-BP on male reproductive system have not been investigated in detail. The present study was conducted to investigate the testicular toxicity of 2-BP and to determine the recovery of normal spermatogenesis in Sprague-Dawley rats. Male rats aged 5 weeks were administered 1,000mg/kg 2-BP by gavage daily for 4 weeks and sacrificed sequentially at 1, 2, 3, 4 and 12 weeks after initiation of 2-BP treatment. Testicular toxicity was evaluated qualitatively by histopathological examinations and quantitatively by reproductive organ weights, spermatid head count, and repopulation index. In the 2-BP treated rats, the body weights was significantly suppressed and the weights of testes and epididymides were also decreased in a time-dependent manner. On histopathological examination, spermatogonia in stages I-VI and preleptotene and leptotene spermatocytes in stages VII-IX were strongly depleted at 1 week of dosing. Spermatogonia were depleted extensively in all spermatogenic stages at 2 weeks. Continuing with the evolution of spermatogenic cycle, zygotene spermatocytes, pachytene spermatocytes, and round spermatids were sequentially depleted at 2, 3, and 4 weeks of dosing due to the depletion of their precursor cells. Vacuolization of Sertoli cells and spermatid retention were also observed at all time points, suggesting that 2-BP induced Sertoli cell dysfunction. At 12 weeks, after 8 weeks recovery, most of the tubules appeared severely atrophic and were lined by Sertoli cells only. Leydig cell hyperplasia in the interstitial tissue was also found. In addition, dramatic reductions in the number of spermatid heads and repopulation index were observed, indicating that 2-BP-induced testicular injury is irreversible. These results indicate that 4 weeks repeated-dose of 1,000mg/kg 2-BP results in a progressive germ cell loss due to the depletion of spermatogonia followed by long-term testicular atrophy in SD rats.

멕시코의 신의 도시 〈2〉

김종춘 한국자동차공학회 2018 오토저널 Vol.40 No.7

비글견에 있어서 새로운 안트라싸이클린계 항암제 DA-125의 정소독성연구

김종춘,차신우,송시환,정문구,Kim, Jong-choon,Cha, Shin-woo,Song, Si-whan,Chung, Moon-koo 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.2

To assess the testicular toxicity induced by DA-125, a new anthracycline anticancer agent, the test substance was intraveneously administered to male beagle dogs at dose levels of 0, 0.0023, 0.0375, 0.15, and 0.6 mg/kg/day, 6 days a week for 26 weeks. At 0.6 mg/kg/day, 1 out of 3 dogs had died on day 42 of treatment and the other dogs were sacrificed on days 46 and 122 of treatment due to the increasingly severe clinical condition. Clinical signs considered to be related to treatment were included anorexia, vomiting, salivation, decreased activity, mucous and/or dark faeces, diarrhea, and swelling, abscess and/or ulceration of injection sites. Suppression in body weight gain, reduction in food intake, decreases in testicular weight and size, and hemorrhage of epididymis were also observed in male dogs. Microscopically, severe degenerative changes such as atrophy of seminiferous tubules, loss of germ cells, degeneration of germ cells, vacuolization of Sertoli cells, and hyperplasia of Leydig cells were observed in all dogs. Azoospermia in epididymal tubules, atrophy of epithelia in the cauda epididymis, and prostate atrophy were also found. At 0.15 mg/kg/day, anorexia, vomiting, salivation, diarrhea, and swelling of injection sites were observed. In addition, suppression in body weight gain and decreases in testicular weight and size were found in male dogs. Atrophy of seminiferous tubules, decrease of germ cells, degeneration, exfoliation and retention of germ cells, vacuolization of Sertoli cells, and hyperplasia of Leydig cells were observed by histopathological examination. Azoospermia in epididymal tubules and prostate atrophy were also found. At 0.0375 mg/kg/day, there were no clinical signs considered to be indicative of a reaction to treatment, but testicular size was significantly reduced. Microscopically, decreases in the number of spermatogonia and epidydimal speramtozoa were found. There were no evidences of general or testicular toxicity at 0.0023 mg/kg/day. These results indicate that DA-125 produces significant and persistent damage to the spermatogenic compartments of the testes in male beagle dogs.

Single dose toxicity study of CKD-602, a new camptothecin anticancer agent, in Beagle dogs

김종춘,신동호,박승춘,손우찬,차신우,한정희,배주현,서정은,정문구,Kim, Jong-Choon,Shin, Dong-Ho,Park, Seung-Chun,Son, Woo-Chan,Cha, Shin-Woo,Han, Junghee,Bae, Joo-Hyun,Suh, Jeong-Eun,Chung, Moon-Koo The Korean Society of Veterinary Science 2004 大韓獸醫學會誌 Vol.44 No.1

The present study was carried out to investigate the potential acute toxicity of CKD-602 by a single intravenous dose in Beagle dogs. The test chemical was administered intravenously to male and female Beagle dogs at dose levels of 0.3, 0.5, or 2.5 mg/kg. Mortalities, clinical findings, and body weight changes were monitored for the 14-day period following the administration. At the end of 14-day observation period, all animals were sacrificed and complete gross postmortem examinations were performed. All males and females of the 2.5 mg/kg dose group were found dead between the fourth and seventh day after the injection. Treatment related clinical signs, including vomiting, anorexia, mucous stool, diarrhea, and no stool were observed. Decrease or suppression of body weight was observed in a dose-dependent manner. In autopsy, dark red discoloration of the gastrointestinal tract, atrophy of the thymus, paleness of the spleen, sporadic dark red spots of the lung and petechia of the heart were observed in dead animals of the 2.5 mg/kg dose group. There were no specific adverse effects on males and females of the 0.3 and 0.5 mg/kg dose groups, except for the transient clinical signs such as anorexia, vomiting, and mucus/no stool. On the basis of the results, it was concluded that a single intravenous injection of CKD-602 to Beagle dogs resulted in increased incidence of abnormal clinical signs and death, decreased body weight, and increased incidence of abnormal gross findings. The absolute toxic dose of this chemical was 2.5 mg/kg for both genders. The $LD_{50}$ value was 1.1 mg/kg (95% confidence limit not specified) for both genders. The no-observed-effect level (NOEL) was considered to be below 0.3 mg/kg for both genders.

Integration of ENIE (English Newspaper In Education) as a Source of Language Input and a Medium for Extensive Reading Program

김종춘 사단법인 한국융합기술연구학회 2024 아시아태평양융합연구교류논문지 Vol.10 No.2

Today, when English proficiency is vital globally, innovative teaching resources that effectively portray real-life English use are needed. This urge is strongest in non-English-speaking countries. This problem can be solved by using English Newspapers in Education (ENIE) as a language input and Extensive Reading (ER) medium, notably in Korean English as a Foreign Language context. This study aimed to investigate the integration of English Newspapers in Education (ENIE) as a source of language input and a medium for Extensive Reading (ER) within the South Korean EFL context. The research involved 50 intermediate level of university nursing students in an English for Specific Purposes (ESP) class over a 10-week period, utilizing a mixed-method approach that included a questionnaire and interviews. Results from the questionnaire and inverviews indicated a generally positive response to ENIE, with increased reading ability, autonomy, and engagement reported by the majority of students, despite some challenges such as language complexity and cultural relevance. They indicate a generally positive reception, with the majority (68%) reporting improvements in reading ability, 84% acknowledging English newspapers as a beneficial source of language input, and 88% expressing interest in continuing to engage with online English newspaper articles independently. The findings suggest that ENIE can significantly contribute to language learning when tailored to students' levels and interests, combined with adequate teacher training and resource curation. This study investigates the incorporation of English Newspapers in Education (ENIE) into the Korean EFL curriculum, aiming to bridge the gap between traditional language instruction and the demands of global communication and literacy, by examining its effectiveness in enhancing extensive reading skills and providing authentic language exposure for educators, curriculum developers, and language learners. The results should inform pedagogical tactics, make English language teaching more engaging and relevant, and give Korean students the linguistic and critical thinking abilities they need for global interactions.

Analyzing the Impact of Book Reports on Reader Proficiency and Fluency in Extensive Reading Program

김종춘 사단법인 한국융합기술연구학회 2024 아시아태평양융합연구교류논문지 Vol.10 No.3

This study aimed to investigate the effectiveness of book reports in learning portfolios in the context of English as a Foreign Language (EFL) education in Korea. Driven by a mixed-methods research design, the study combined quantitative assessments with qualitative feedback to explore the impact of book reports on learners' reading competency and fluency. The study involved 83 participants and the intervention spanned 15 weeks, with the students having access to an extensive selection of 461 graded books available both digitally and in print. The study assessed the impact of book reports on critical reading measures, including TOEIC RC (Test of English for International Communication Reading Comprehension), Accelerated Reader (AR), Reading Speed, and Reading Speed Comprehension. It aimed to identify differences in results between two different teaching approaches: Educator-facilitated and Learner-initiated. In addition to quantitative evaluations, the study integrated input from participants, collected through questionnaires and interviews, to offer a comprehensive perspective on the impact of book reports on enhancing reading ability and fluency. The key findings indicate that book reports significantly enhance reading ability with notable variances observed between the two teaching approaches. The educator-facilitated approach yielded more pronounced improvements, which suggest that the integration of book reports in extensive reading program can be a potent tool in EFL settings, with different methodologies catering to varied learning objectives. By integrating these diverse perspectives, the study result, in terms of implication, aspires to offer insights into the value and impact of book reports, thus contributing to the optimization of learning portfolios for the enhancement of reading skills among EFL learners.