http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
송시환,김형진,신천철,임광현,하창수,한상섭 한국독성학회 1998 Toxicological Research Vol.14 No.2
KH-502 (Flupyrazofos), a new organophosphorus insecticide synthesized by Korea Re-search Institute of Chemical Technology, was found to be effective against diamond-back moth(Plutella xylostella). This study was carried out to determine the acute toxicity of KH-502 in Sprague-Dawley rats and ICR mice. The test article was orally or dermally administered to the animals. Death, tremors, salivation, lacrimation, abnormal gait and corneal opacity were observed. Decrease in body weight gain was observed in all treatment groups. At necropsy, dark red coloration of lung, enlargement of adrenal glands and atrophy of spleen were observed. The oral $LD_{50}$ value was 372 mg/kg in male rats, 605 mg/kg in female rats, 186 mg/kg in male mice, and 115 mg/kg in female mice. And the dermal $LD_{50}$ was 4086 mg/kg in male and 3881 mg/kg in female rats.
송시환,양덕춘,정세영 한국식품위생안전성학회 2004 한국식품위생안전성학회지 Vol.19 No.4
산삼배양추출물의 세균에서의 돌연변이 유발성 검색을 위하여 Salmonella typhimurium의 히스티딘 요구성 균주 TA100, TA1535, TA98 및 TA1537의 4개의 균주와 대장균 Eschericha coli의 트립토판 요구성 균주인 WP2 uvrA를 이용해 복귀돌연변이 시험을 실시하였다. 시험물질은 멸균생리식염수에 용해하여 처리하였다. 대사활성계 적용 및 미적용시 모든 균주에 대해 0, 62, 185, 556, 1,667 및 5,000 μg/plate의 범위를 설정하고 각각 음성 및 양성대조군으로 시험군을 구성해 본 시험을 실시하였다. 시험 결과 모든 균주에서 최고농도에 이르기까지 집락수의 일관성 있는 증가는 나타나지 않았다. 이상의 결과를 종합할 때, 시험물질 산삼배양추출물은 본 시험조건 하에 사용한 시험균주들의 복귀돌연변이를 유발하지 않는 것으로 사료된다. To evaluate the bacterial reverse mutation of wild ginseng culture extract, the in vitro Ames test using Salmonella typhimurium (TA100, TA1,535, TA98, TA1,537) and Escherichia coli (WP2 uvrA) were performed with wild ginseng extract at the concentrations 0, 1.6, 8, 40, 200, 1,000, 2,500 and 5,000μg/ml/plate. Wild ginseng culture extract was negative in Ames test with both Salmonella typhimurium or Escherichia coli with and without rat liver microsomal enzyme (S-9 fraction). According to these results, we concluded that wild ginseng culture extract did not cause bacterial reverse mutation.
산삼배양추출물의 ICR 마우스 골수세포를 이용한 복강 투여 소핵시험
송시환,양덕춘,정세영 한국식품위생안전성학회 2005 한국식품위생안전성학회지 Vol.20 No.1
시험물질 산삼배양추출물의유전독성 평가를 위해 수컷 ICR 마우스 골수세포를 이용한 소핵시험을 실시하였다. 1회 투여 최고량은 예비 시험에서 결정하였다. 약 7주령의 수컷 마우스에 시험물질 0, 500, 1,000 및 2,000 mg/kg의 용량을 1일 1회 2일간 복강내 투여하고, 최종 투여로부터 약 24시간 후에 골수세포를 수거하여 소핵 유발과 세포독성을 평가하였다. 개체당 2,000개의 다염성적혈구(michromatic crythrocyte, PCE)중에 나타나는 소핵을 가진 다염성 적혈구(micronucleated polychromatic erythrocyte, MNPCE)의 수를 게수한 결과, 모든 시험물질 투여군은 음성 대조군에 비해 통계학적으로 유의한 증가는 나타나지 않았으며 일반 증상에서도 모든 시험군은 투여로 인한 것으로 판단되는 증상은 관찰되지 않았다. 부검시 체중에 있어서는 시험물질 최고 용량군에서 유의한 감소가 관찰되었다. 따라서 산삼배양추출물은 위 시험 조건에서, 본 시험에 사용한 마우스 골수세포에 소핵을 유발하지 않는 것으로 사료된다. To assess elastogenic effects of the wild ginseng culture extract (WGCE) is vivo micronucleus test was performed using 7 weeks old ICR mice. At 24 hours after 2nd treatment with wild ginseng culture extract at the doses of 0, 500, 1,000, and 2,000 mg/kg/day by peritoneal route mice were sacrified and marron cells were prepared for smear slides. As a result of counting the micronucleated polychromatic erythrocyte (MNPCE) of 2,000 polychromatic erythrocyte(PCE), all treatment groups did not show statistically significant increase than negative control group. And there was no clinical sign connected with injection of wild ginseng culture extract. It was concluded that wild ginseng culture extract did not induce micronucleus in the marrow cells of ICR mice.
Methylcellulose의 경구 및 정맥내 반복 투여가 SD랫드의 간장, 비장 및 신장에 미치는 독성학적인 영향
송시환,강부현,한상섭,노정구,이창업,Song, Si-whan,Kang, Boo-hyun,Han, Sang-seop,Roh, Jung-koo,Lee, Chang-eup 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.1
This experiment was carried out to study the toxic effect of solublized methylcellulose (MC). Sprague-Dawley rats were dosed with 1%(w/v) MC in 0.9% saline by gavage at a dose of 10ml/kg b.w/day or by intravenous injection at a dose of 5ml/kg b.w/day for 28 days. Clinical signs were observed once a day. Body weights, water and food consumptions were measured and urinalysis was performed several times during the experiment. Rats were sacrificed on days 3, 7, 15 and 28 for hematology, blood chemistry, organ weights and histopathology. The relative weight of the spleen and foamy cells of the spleen were increased in the gavage group. Body weight gain, food consumptions, the values of RBC, Hb, MCH, Hct, serum proteins, glucose, bilirubin, AST, and ALP were decreased in I.V. treatment group. On the other hand, water consumptions, the values of serum cholesterol, creatinine, and BUN were increased. Microscopic findings were granulomas, distended sinusoids, and hypertrophy of Kupffer cells with vacuoles in the liver. Spleen exhibited granuloma, increased extramedullary hematopoiesis, and congestion. Kidney exhibited foamy cells in the glomeruli, distension of the tubules. The findings appeared more severe when the treatment was extended. In conclusion, MC solution is not a safe vehicle for intravenous administration because of the toxic effects on the liver, kidney and spleen. In addition, a long-term and large dosage of oral administration of MC appears to be unsafe also and needs to be investigated further.
산삼배양추출물의 비글견을 이용한 단회 경구투여 독성시험
송시환(Si-Whan Song),양덕춘(Deok Chun Yang),정세영(Se Young Choung) 한국독성학회 2005 Toxicological Research Vol.21 No.1
To investigate the acute toxicity of adventitious roots extract derived from wild ginseng, it was orally administered to beagle dogs with a single dose. In acute toxicity test, three groups (9 beagle dogs of male) were administered with different dosages of adventitious roots extract (prepared by Biopia Corp.) 500 mg/kg (G2), 1,000 mg/kg (G3), 2,000 mg/kg (G4) and one group (G1, 2 beagle dogs of male) were received by only capsule without the extract according to the Regulation on Korea Food and Drug Administration (1999. 12. 22). There were vomitus for a time and mucous stool at the day, and anorexia and mucous stool at the first day in the group of 2,000 mg/kg administration. There were mucous stool in one and anorexia for a while in two beagle dogs at the first day in the 1,000 mg/kg administration. But no death or abnormal clinical sign was observed through the study period. Therefore, the adventitious roots extract derived from wild ginseng is considered not to have the acute toxicity in the beagle dogs. These results suggest that LD_(50) value of the test substance was considered to be more than 2,000 mg/kg in the beagle dogs.
게르마늄 복합물인 STB-HO-BM에 대한 유전독성에 관한 연구
송시환(Si Whan Song),정연권(Winston Jung),홍동호(Dong Ho Hong) 한국독성학회 2006 Toxicological Research Vol.22 No.2
We have investigated the genotoxicity of STB-HO-BM using in vitro and in vivo system such as Ames reverse mutation test, chromosomal aberration test and micronucleus test. in Ames reverse mutation test, STB-HO-BM treatment at the dose range up to 5,000 ug/plate did not induce mutagenicity in Salmonella typhimurium TA98, TA100, TA102, TA1535, TA1537 and in Escherichia coli WP2 uvrA with and without metabolic activation. Any significant aberration wasn’t observed in chinese hamster lung (CHL) fibroblast cells treated with STB-HO-BM at the concentration of 12.5, 2.5, 5 ㎎/㎖ both in the absense and presence of metabolic activation system. In mouse micrnucleus test, no significant increase in the occurrence of micronucleated polychromatic erythrocytes was observed in ICR male mice orally administered with STB-HO-BM at the doses of 0.5, 1.0, 2.0 g/㎏. These results indicate that STB-HO-BM has no mutagenic potential under the condition in this study.
게르마늄 복합물 STB-HO-BM의 랫드 및 비글견에서 단회투여 독성연구
송시환(Si Whan Song),정연권(Winston Jung),홍동호(Dong Ho Hong) 한국독성학회 2006 Toxicological Research Vol.22 No.2
The acute toxicity of STB-HO-BM was evaluated in Sprague Dawley (SD) rats and beagle dogs. STB-HO-BM was administered orally to rats at dose levels of 0 and 2,000 ㎎/㎏/day and to dogs at dose levels of 0, 500, 1,000 and 2,000 ㎎/㎏/day. In these experiments, there were no death and clinical changes which were related to STB-HO-BM administration. In addition, there were no significant changes between control and treated groups in body weights and autopsy findings. In conclusion, the administration of STB-HO-BM 2,000 ㎎/㎏ in SD rats and up to 2,000 ㎎/㎏ in beagle dogs was proved to be safe, and it is thought that STB-HO-BM may not show any toxicity in its clinical use.