기관지천식 환아에서 면역요법에 따른 특이 IgE , 기도과민증 및 부비동염의 변화

김소연(Soh Yeon Kim),이준성(Joon Sung Lee) 대한천식알레르기학회 1999 천식 및 알레르기 Vol.19 No.2

Background: Though immunotherapy(IT) has become an effective rnethod in extrinsic allergic patients who didn't respond to pharmacologic therapy or couldn't avoid allergen, the mechanism, termination index and prognostic index of IT have not been clarified yet. Methods: We selected 81 asthmatic children on immunotherapy with house dust mite (Dermatophagoides pteronyssinus and Dermatophagoides farinae). We measured the hematologic findings, the levels of serum IgG and IgE, allergen(house dust, Dermatophagoides pteronyssinus and Dermatophagoides farinae)-specific IgE concentrations, lymphocyte subsets and methacholine challenge test yearly during IT, and checked the radiographs of chest and paranasal sinus. Results: Peripheral white blood cell count, the percentage of eosinophil and total eosinophil count decreased significantly after 2 years of IT. Serum IgG level increased significantly after 3 years of IT. Serum total and specific IgE levels decreased significantly after 3 years of IT, but they were still higher than the normal values. CD4+, CD8+, and B lymphocytes did not change with the IT, but CD3+ lymphocytes increased significantly after 2 years of IT. PC20-methacholine increased significantly after 1 year of IT, but no correlation was found between the duration of IT and bronchial hyperreactivity. Twenty-eight patients(34.6%) had abnormal findings on chest radiographs: 15 patients(53.6%) as bronchitis, 10 patients(35.7%) as bronchopneumonia, 2 patients(7.1%) as hyperinflation and 1 patient(3.6%) as atelectasis. Sixty-three patients(77.8%) had abnormal findings on paranasal sinus radiographs. In the follow-up radiographs of 49 patients, 28 patients(57.1%) showed improvement of paranasal sinusitis after 1 year of IT. Conclusion: This study showed some changes of the immunologic findings such as eosinophil count, IgG, IgE, allergen-specific IgE and CD3+ lymphocytes, and improvement of bronchial hyperreactivity and paranasal sinusitis' in asthmatic children during IT. These findings were closely related to clinical improvement.

공공임대아파트의 사회적기업 운영 상가에 대한 실태조사

김화영(Kim, Hwa-Young),김소연(Kim, Soh-Yeon),윤지원(Yun, Ji-Won),박경옥(Park, Kyoung-Ok) 한국주거학회 2018 한국주거학회 학술대회논문집 Vol.30 No.2

원룸형 다가구주택 단위주호의 저녁시간대 실내공기질 측정조사

김도경(Kim, Do-Gyeong),김소연(Kim, Soh-Yeon),최윤정(Choi, Yoon-Jung) 한국주거학회 2017 한국주거학회 학술대회논문집 Vol.29 No.1

소아 골수이형성 증후군에서 조혈모세포이식의 단기간 결과 분석

이진,김소연,조빈,장필상,정낙균,김학기,Lee, Jin,Kim, Soh Yeon,Cho, Bin,Jang, Pil Sang,Chung, Nak Gyun,Kim, Hack Ki 대한소아청소년과학회 2002 Clinical and Experimental Pediatrics (CEP) Vol.45 No.3

목 적 : 소아의 골수이형성 증후군은 드문 질환군으로 예후가 매우 불량하며 화학요법으로는 완치가 어렵다. 유일한 완치요법으로서 조혈모세포이식이 시행되고 있으나 소아의 경우 증례가 적어 이에 대한 체계적 결과 분석이 빈약한 실정이다. 저자들은 골수이형성증후군에서 조혈모세포이식을 시행한 증례들의 단기간의 결과와 이식합병증들을 분석하고자 하였다. 방 법 : 1995년 11월부터 2001년 1월까지 가톨릭대학교 성모병원 소아과에서 골수이형성 증후군으로 조혈모세포이식을 시행 받은 10명의 환아를 대상으로 하였다. 대상질환은 CMMoL 5례, RAEB 3례, RAEBt 2례이었고, 이식형태는 HLA-일치 형제간 골수이식 4례, 비혈연간 골수이식이 4례, 제대혈 조혈모세포이식이 1례, 가족간 HLA-부분일치 조혈모세포이식이 1례이었다. 전처치로는 BuCy 5례, TBI+BuCy 2례, BuCy+ATG, TBI+Cy 및 TBI+Melphalan이 각각 1례에서 사용되었다. 결 과 : 1) 10명 모두 생착(100%)되었으며 현재 8명(80%)이 무병생존(3-65개월, 정중 추적기간 11개월) 중이다. 2) 이식전처치로 인한 합병증으로 VOD가 3례에서 관찰되었으나 사망한 예는 없었다. 3) II-III도의 급성 이식편대 숙주병은 5례(50%)에서 발생하였으며 II가 4례, III가 1례이었다. 급성 이식 편대 숙주병과 관련된 사망은 없었다. 4) 전체 환아 10례 중 3례에서 이식 후 재발되었으나 1례는 화학요법 후 조혈모세포구제술에 의하여 현재 무병생존 중이며 2례는 사망하였다. 결 론: 소아 골수이형성 증후군에서 조혈모세포이식은 질환을 완치시킬 수 있는 우수한 결과를 보여주고 있으나 아직 증례가 적고 추적기간이 짧아 향후 더 많은 연구가 필요하다. Purpose : In most cases, myelodysplastic syndrome(MDS) transforms into a more aggressive state or acute myelogenous leukemia; it's prognosis is very poor. It is believed that hematopoietic stem cell transplantation(HSCT) is the only curative treatment of MDS, but available data in children are very sparse. In this report, the short term outcome of HSCT in childhood MDS was analyzed. Methods : Ten children with MDS(CMMoL 5, RAEB 3, RAEBt 2) underwent HSCT(HLA-matched sibling transplantation 4, HLA-matched unrelated transplantation 4, cord blood transplantation 1, HLA-mismatched familial transplantation 1) between November 1995 and January 2001 at St. Mary's Hospital. Median follow-up duration was 11 months. Results : Engraftment was successful in all cases and 8 patients are alive without disease. Three cases of VOD were observed and improved without complication. Four cases of grade II and 1 case of grade III acute GVHD were observed and well controlled with treatment. Three patients relapsed after transplantation. One patient is alive without disease after cytoreduction with allogenic stem cell rescue and 2 patients died of relapse. Conclusion : HSCT is a curative strategy of MDS and the survival rate is relatively higher than that of adults. But there is an obvious need for more studies because of the small number of patients and the short duration of the follow-up.

중소도시 주말텃밭 이용자의 참여동기와 이용만족에 관한 연구: 전주시 사례

황영모(Young Mo Hwang),김소연(Soh Yeon Kim) 한국농촌사회학회 2013 農村社會 Vol.23 No.2

이 연구는 도시민의 생활농업과 농업인의 생업농업이 교류를 통해 만나는 접점으로 중소도시 주말텃밭에 주목하고 있다. 특히 주말텃밭의 발전방향은 공급측면보다 수요측면의 중요도가 높다는 점을 전제로 하였다. 이러한 관점에서 이 연구는 전주시 주말텃밭 이용자를 대상으로 주말텃밭 이용자의 욕구와 만족도 관계를 분석하고, 주말텃밭 운영 활성화에 시사점을 도출하고자 하였다. 주말텃밭 이용자의 참여동기 요인은 ‘여가휴식, 농촌향수, 식품안전, 커뮤니티, 가족정 서공유’ 등으로 분석되었다. 이중 식품안전 및 여가휴식 동기가 타 참여동기에 비해 높게 나타났다. 주말텃밭 이용자의 만족도 요인은 ‘재배환경, 운영자 태도, 부대시설, 농장기반’ 등으로 추출되었다. 이중 농장기반을 제외하고 나머지는 부족한 것으로 평가되었다. 참여동기와 만족도 요인은 개인 특성별로는 큰 차이가 없는 것으로 나타났다. 따라서 기대하는 목표에 구체적으로 부응하거나 부대시설 및 운영자 태도 개선 등의 주말텃밭 운영방안을 모색하는 것이 요구된다. 주말텃밭 이용자의 참여동기와 이용 만족도 간의 관계를 분석한 결과 생산-문화 관계성과 가족실리 추구의 강약이 참여동기와 이용 만족도 형성에 관여되는 속성이라는 것을 확인하였다. This study focuses on weekend farms in small and medium-sized cities, which play a role for a contact point between urban agriculture and agriculture for making a living. Especially, the direction for developing weekend farms is based on the fact that demand is more important than supply. In this regard, this study analyzed the relations between the desire of weekend farm users and their satisfaction among the weekend farm users in Jeonju-si and then drew implication on facilitation of the operation of weekend farms. Weekend farm users are led by the following motivations: rest and recreation, nostalgia for countryside, food safety, community activities, and sharing sentiments with family members. Among such motivations, food safety and rest and recreation account for a high portion. Users’ satisfaction factors are cultivation environment, response of operators, auxiliary facilities and basis of farm. Except for the basis of farm, the rest are insufficient to satisfy the users. Participation motivation and satisfaction factors do not show any significant difference by individual characteristics. Thus, it is required to seek ways to operate weekend farms in a successful manner, such as specifically responding to expected goals and improving behavior of persons operating farms and auxiliary facilities. As a result of analyzing the relations between the participation motivation and satisfaction levels, we confirm that the relations with production-culture and strength of pursuing family interests are involved in forming a participation motivation and improving satisfaction.

녹색 형광단백 유전자를 함유한 아데노바이러스 주입시 마우스 생체내 발현양상

진종률(Jong Youl Jin),송치원(Chi Won Song),김진아(Jea Na Kim),이희진(Hee Jin Lee),김태규(Tai Gyu Kim),한치화(Chi Wha Han),엄현석(Hyun Seok Eom),박수정(Soo Jeong Park),정대철(Dae Chul Jeong),정낙균(Nak Gyun Chung),김소연(Soh Yeon Kim) 대한내과학회 2001 대한내과학회지 Vol.61 No.5

Background : The green fluorescent protein (GFP) from jelly fish, A equorea victoria, has become a versatile reporter for monitoring gene expression in a variety of cells and organisms . Using GFP as a marker protein we studied whether there are any differencies in the expression patterns among organs in mouse after intravenous injection of adenovirus vectors with GFP gene. Methods : Recombinant E1, E3- defective type 5 adenovirus vectors (2×10(8)/mouse) with CMV promoter and GFP gene were injected into mice via tail vein. On 3, 6, 9, 14, 21, 28 days after gene transfer, 5 mice per experiment s were sacrificed by cervical dislocation and obtained liver , lung, heart , kidney, spleen, small intestine and bone. Half of them were examined by optical microscope after H-E stain. Another half were examined by fluorescent microscope after frozen section. Western blotting were done for each samples with anti- GFP monoclonal antibody and obtained GFP bands were quantitatively compared using Gel-Doc (Bio- Rad, USA) image analyzer. Results : In all organs that we obtained, expression of GFPs are noticed 3 days after gene transfer and reached a maximum around 9th to 14th days, after then the intensities are slightly decreased but maintained until 28th days as determined by Western blotting. On fluorescent microscopic examination, GFPs are well and most frequently expressed on lung among all the examined organs. There are little expression of GFPs on liver parenchymal area around the sinusoids and central veins, although patchy expression of GFPs are observed along the liver capsules. GFPs are highly expressed around the splenic trabecula area but splenic pulp area, it is very spar sely expressed. GFPs are more frequently and highly expressed around the renal tubular area than gromerular area in kidneys. In small intestine, GFPs are expressed on mid portion of microvilli. GFPs are not expressed on myocardium except scanty expression on endocardium. Bone marrow showed GFPs but precise localization is difficult because bony spicules mashed bone marrow during the preparation of frozen section. No specific pathologic lesions possibly related with adenovirus administration are observed on microscopic examination of H-E stained specimens. Conclusions: GFPs can be detected in cells without the fixing and staining and a good marker to studying the kinetics and persistence of adenovirus mediated gene therapy. And there are different GFP expression patterns according to the organs after intravenous injection of adenovirus vectors with GFP gene in mouse.(Korean J Med 61:537- 545, 2001)

L'Aspriration vers l'infini dans les Memoires d'outre-tombe

김소연 德成女子大學校 1988 德成女大論文集 Vol.17 No.-

Chateaubriand savait prendre une reconfortante hauteur au-dessus des terrestres miseres. Il a dit que l'individualite hamaine avait servi a mesurer la petitesse des plus grands evenements. Il avait l'obsession du Temps et de la Mort. Ce queteur incessant d'absolu, d'immensite, d'infini a incarne la passion de l'independance, le souci de la dignite individuelle avec son style superbe. Il ne cesse de recourir pour figurer un Espace et un Temps sans limites. Les mots caracteristiques des Chateaubriand sont mer, abime, desert, immensite, desirs, chimeres, songes, souvenirs. Tous sont lies a l'image de l'espace. Sa voluminosite des etats de l'ame trovent dans les images spatiales leur decor. Son ame est situe au centre d'une immensite. Et Chateaubriand construit sa phrase comme une serie de groupes ou de membres d'etendue croissante, quand il veut figurer un espace qui s'elargit, une duree, un mouvement qui se prolongent indefiniment.

선천성 순적혈구 형성부전 환아에서 시행한 동종 골수이식 1예

이현정,김소연,정낙균,조빈,정대철,김학기 대한조혈모세포이식학회 1999 대한조혈모세포이식학회지 Vol.4 No.2

선천성 순적혈구 형성부진은 드문 질환으로 심한 빈혈, 망상적혈구 감소와 골수에서 과립구 형성 및 거핵구 형성은 정상이면서 적혈구계의 형성만 선택적인 부전이 일어나는 질환이다. 이렇나 적혈구 전구 억제의 이유는 erythropoietin(Epo), interleukin-3 (IL-3) 같은 cytokine이나 적혈수 상의 그 수용체의 유전 인자적인 결함일 수 있으나 아직까지 연구결과가 분명하지 않다. 치료는 부신피질 스테로이드 단독, 혹은 cyclophosphamide, azathioprine, cyclosprine, 또는 ATG나 ALG같은 면역억제제의 병용투여가 시행되고 있다. 그러나 적극적인 면역억제 치료에도 불구하고 재발하거나 치료에 불응성인 환자들의 경우 지속적인 적혈구 수혈에 의존하게되며 이러한 경우 바이러스 감염 등 수혈과 연관된 혈액전파, 감염, 헤모시데린 침착증 등에 의한 전신 장기부전 등의 부작용이 나타나 환자의 생존에 심각한 영향을 미치게 된다. 저자들은 치료불응성 선천성 수적혈구 형성부전 환아에서 국내에서는 처음으로 전신 방사선조사와 고용량의 cyclophosphamide로 전처치 후 HLA-일치 동종 골수이식을 시행하여 성공적으로 치료한 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Congenital pure red cell aplasia (PRCA) is characterized by anemia, reticulocytopenia, and erythroid hypoplasia without abnormalities in the myeloid and megakaryocytic series. In most cases of PRCA, the erythroid progenitor cell is intrinsically abnormal in the following respects: (1) decreased sensitivity to erythropoietin (EPO); (2) decreased sensitivity to interleukin-3 (IL-3). Corticosteroid therapy is frequently beneficial if it begins early. The mechanism of its effect is unknown. Other therapies, including androgens, cyclosporin A, cyclophosphamide antithymocyte globulin (ATG), high dose intravenous immunoglobulins, erythropoietin, and interleukin-3(IL-3) have not had a consistent beneficial effect and may have a high incidence of side effects. For PRCA patients with refractory to conventional treatments, disease morbidity is increased and life expectancy is significantly shortened because of increased infection risk or multiorgan failure due to chronic immune suppression status and prolonged transfusions. We report our experience that the high dose immune suppression with cyclophosphamide (50 mg/kg/day, for 4 day) and total body irradiation (TBI) followed by allogeneic bone marrow transplantation for refractory PRCA patient, and he is hematologically remission for 8 months after transplantation. In Korean, we describe the first report of a patient with PRCA who was successfully treated with allogeneic bone marrow transplantation.

영아기 거대세포 바이러스(CMV) 간염 : 혈청내 CMV 특이 IgM 항체와 소변내 바이러스 조기항원 검출에 대한 연구

손영모,조형래,김소연,정기섭,송경순,오기근,이원영 대한감염학회 1990 감염 Vol.22 No.4

Four hundred and three infants with hepatosplenomegaly or abnormal liver function were studied for antibodies of Hepatitis A and B, Rubella, Herpes simplex virus, Cytomegalovirus (CMV), Toxoplasma and Syphilis. Among them, 22 infants were also studied for viruses in urine. Monoclonal antibodies especially, for that of CMV, were used to detect virus encoded early antigen with in the urine and the following results were obtained. Among the 403 infants tested, 37 cases (9.2%) were confirmed CMV infection by detection of CMV specific IgM or viral antigen detection in urine. Among the 22 cases in which virus culture was done in urine, 8 cases (36.4%) were confirmed CMV infection and CMV was isolated in 6 cases (27.3%). Virus isolation rate of confirmed CMV infected cases was 75% (6/8). It can be concluded that hepatitis due to CMV plays a significant role in neonatal hepatitis in Korea.

백혈병 마우스 모델의 동종골수이식에서 활성화된 자연살해세포들의 보충이 이식편대백혈병효과와 이식편대숙주반응에 미치는 영향

엄현석,한치화,박수정,김소연,정낙균,정대철,진종률,최일봉,양형모,서영훈,송현근,최인표,민우성,김춘추 대한조혈모세포이식학회 2001 대한조혈모세포이식학회지 Vol.6 No.1

배경: 백혈병에서 동종골수이식 (allogeneic bone marrow transplantation)의 성공적 치료 효과를 얻기 위해서는 이식편대숙주반응 (graft-versus-host disease, GVHD) 발생의 극복과 재발의 방지가 중요한 과제이다. 골수를 역류원심성 세포분리 (counterflow centrifugal elutriation, CCE) 방법으로 분리하여 얻은 rotor off (R/O) 세포분획은 T 세포의 수는 적지만 조혈모세포들을 다량 포함하고 있어 동종골수이식에서 주조직적합복합체 (major histocompatibility complex, MHC) 차이를 극복할 수 있고, 이식편의 생착 성공과 GVHD 발생 예방에 효과적이다. 그러나 골수로부터 T 세포를 제거하면 백혈병세포를 공격하는 이식편대백혈병 (graft-versus-leukemia, GVL) 효과가 감소되기 때문에 백혈병 재발의 빈도가 높다. 자연살해세포 (natural killer cell, NK cell)의 보충 첨가는 동종골수이식 후 GVHD 발생을 줄이면서 충분한 GVL 효과를 얻을 수가 있다. 따라서 저자는 분리 후 IL-2로 활성화시킨 NK 세포들을 골수 R/O 세포분획과 함께 백혈병 마우스 모델에 동종이식함으로써 GVHD와 GVL에 미치는 효과를 관찰하였다. 방법: Balb/c (H-2^(d)) 마우스에서 유래된 A20 (murine B-lymphoma/leukemia cell line, H-2^(d)) 백혈병 세포를 이식 2 일 전에 Balb/c 마우스에 주입하고, 치사량의 전신 방사선을 조사한 직후에 Balb/c 또는 C57BL/6 (H-2^(b)) 마우스의 골수 R/O 세포분획을 꼬리정맥을 통하여 주입하였다. 이들은 모두 이식 후 6-8 주 이내에 사망하였다. 동종이식의 대조군 (n=9)에는 1 × 10^(7)의 R/O 세포분획만을 주입하였고, 실험군 (n=9)에는 C57BL/6 마우스의 비장세포들로부터 단클론항체들을 이용한 negative selection방법으로 분리한 후 IL-2로 활성화된 5 × 10^(5)의 NK 세포분획을 1 × 10^(7)의 R/O 세포분획과 함께 주입하였다. GVL 효과의 판정은 이식 후 14 일과 28 일 째 되는 날 마우스에서 골수, 비장, 간 등을 얻어 백혈병 세포들의 침윤을 조직학적으로 관찰하였으며, GVHD의 정도는 육안적 관찰법으로 평가하였다. 결과: R/O 세포분획만을 이식한 대조군의 골수, 비장, 그리고 간 조직에서는 A20 백혈병 세포의 침윤이 각각 89% (8/9), 78% (7/9)와 22% (2/9)에서 관찰되었고, R/O 세포분획과 NK 세포분획을 함께 이식한 실험군에서는 비장과 간을 제외한 골수에서만 89% (8/9)에서 A20 백혈병 세포의 침윤이 관찰되어 두 군 사이에 장기별 분포의 차이를 볼 수 있었다 (P= 0.0001). 한편 GVHD는 두 군 모두에서 경하게 나타나서 유의한 차이는 없었다. 또한 생착 부전으로 사망한 마우스는 없었다. 결론: CCE를 이용하여 T 세포를 제거한 동종골수이식에서 NK 세포의 보충은 GVHD의 악화는 일으키지 않으면서, 백혈병의 진행을 억제하는 GVL 효과를 얻을 수 있었다. Background: Allogeneic bone marrow transplantation (BMT) with T cell-depleted marrow accompanies engraftment failure and relapse of leukemia by a loss of the graft-versus-leukemia (GVL) effect frequently, while it can prevent GVHD. Supplement of NK cells could prevent GVHD and enhance GVL effect in several murine allogeneis BMT models Roter off (R/O) cell fraction obtained by counterflow centriation elutriatio (CCO) contains small number of T cells and many hematopoietic stem cells. The aim of this study was to determine the effect of R/O cell fraction supplemented with IL-2 activated NK cells on GVL and GVHD within the leukemic mouse BMT model. Methods: Inoculation of A20 (H-2d, murine B-lymphoma/leukemia, Balb/c origin) cells into Balb/c mice via the tail vein 2 days prior to lethal total body irradiation (TBI) and infusion of the Balb/c BM or C57BL/6 (H-2b) R/O fraction were performed. It resulted in 100% mortality within 6 to 8 weeks. The irradiated mice in the control group were injected with 1 × 107 R/O cell fraction alone (n=9) and in the experimental group mice were injected with 1 × 107 R/O cell fraction plus 5 × 105 negatively selected IL-2 activated NK cell fractions of the spleens via the tail vein (n=9). On day 14 and 28 after BMT, the bone marrows, spleens, and livers of mice were harvested for histopathologic analysis of the infiltrations of leukemic cells. We then evaluated the GVHD within the mice. Results: A histopathologic study of the recipients receiving R/O fraction alone showed infiltration of leukemic cells, 89% (8/9) in bone marrows, 78% (7/8) in spleens, and 22% (2/9) in livers. The experimental group of mice showed only the infiltration of leukemic cells 89% (8/9) in bone marrows, not in spleens and livers. There were the organ differences of the leukemic cells infiltrations between the two groups (P=0.0001). There were no obvious differences in the GVHD scores between these two groups, and severe GVHD was not observed. There was no engraftment failure among groups. Conclusion: Thus, our findings suggest that R/O cell fraction obtained by CCE and supplemented with NK cells can promote GVL effect without mediating clinically overt GVHD in allogeneic BMT of mouse leukemia.