IL-15에 의한 류마티스관절염 환자 활막 섬유모세포에서의 SDF-1 유도

박영은 ( Young Eun Park ),김성일 ( Sung Il Kim ),박성후 ( Seong Hu Park ),백승훈 ( Seung Hoon Baek ),오혜좌 ( Hye Jwa Oh ),허양미 ( Yang Mi Heo ),조미라 ( Mi La Cho ) 대한류마티스학회 2010 대한류마티스학회지 Vol.17 No.3

Objective: Interleukin-15 (IL-15) recruits and activates synovial T cells, and IL-15 plays an important role in amplifying and perpetuating inflammation in the pathogenesis of rheumatoid arthritis (RA). Stromal cell-derived factor-1 (SDF-1) is a potent chemoattractant for memory T cells in the inflamed RA synovium. This study investigated the effect of IL-15 on SDF-1 production in RA fibroblast-like synoviocytes (FLS). Methods: The expressions of IL-15 and SDF-1 were determined from the synovium of patients with RA and osteoarthritis (OA) by performing immunohistochemistry. The expressions of SDF-1 was measured from the RA FLS that were cultured with IL-15 and IL-17 by real-time RT-PCR and ELISA. The SDF-1 expression was also measured, via ELISA, from the RA FLS stimulated by IL-15 together with the inhibitors of such intracellular signal molecules as phosphatidylinositol 3-kinase (PI 3-kinase, LY294002), STAT3 (AG490), MAP Kinase (PD98059), NF-κB (parthenolide) and activator protein 1 (AP-1, curcumin). Results: IL-15 and SDF-1 were mainly expressed in the RA synovium compared to that of the OA synovium. IL-15 increased the SDF-1 expressions and it, and had an additive effect with IL-17 on the SDF-1 expressions in the cultured RA FLS. The IL-15 induced increase of the SDF-1 expression in the cultured RA FLS was blocked by the inhibitors of PI 3-kinase, NF-κB and AP-1. Conclusion: The SDF-1 expression was increased in the RA synovium and it was up-regulated by IL-15 in the RA FLS through the PI 3-kinase, NF-κB, and AP-1 pathways. These results imply that the IL-15 induced increase of the SDF-1 expressions may be involved in the immunopathogenesis of RA.

류마티스 관절염 동물 모델에서 활막의 RANKL/OPG mRNA 발현 비율 및 IL-17의 효과

이준희 ( Jun Hee Lee ),김근태 ( Geun Tae Kim ),류선 ( Sun Ryu ),김주인 ( Ju In Kim ),백승훈 ( Seung Hoon Baek ),김성일 ( Sung Il Kim ) 대한류마티스학회 2006 대한류마티스학회지 Vol.13 No.2

Objective: To investigate the synovial mRNA expression of receptor activator of NFκB (RANK), RANK ligand (RANKL), osteoprotegerin (OPG) and RANKL/OPG mRNA expression ratio, and to evaluate the effects of IL-17 in experimental rheumatoid arthritis (RA) model. Methods: After induction of collagen-induced arthritis (CIA) by type II collagen in DBA1 mice, mice were anesthetized at day 28 and a small aperture in the skin of the knee was performed. Mice, in which arthritis of knee was present, were selected and divided into 3 groups, and phosphate-buffered saline (PBS group), IL-17 (IL-17 group) or anti-IL-17 monoclonal antibody (anti-IL-17 group) was injected to both knee joint at day 28 and 32. At day 35, mice were sacrificed and synovium of knee joints were isolated. Synovial mRNA expression of RANKL, RANK and OPG was assessed by real-time RT-PCR and immunohistochemical stain. Results: Synovial RANKL and RANK mRNA expressions were significantly different among IL-17, PBS, anti-IL-17 and normal group (IL-17>PBS>anti-IL-17>normal group), and synovial OPG mRNA expressions in PBS, IL-17 and anti-IL-17 group were significantly high than those in normal group, however, there was no significant difference among IL-17, PBS and anti-IL-17 group. RANKL/OPG mRNA ratio was significantly different among these groups (IL-17>PBS>anti-IL-17>normal group). In immunohistochemical stain, RANKL, RANK and OPG-positive cells were expressed at synovium. Conclusion: Synovial RANKL/OPG mRNA ratio was enhanced in CIA, and IL-17 induced higher RANKL/OPG ratio in the synovium of CIA, which was blocked by anti-IL-17 antibody. These results suggest that RANKL/OPG mRNA ratio play an important roles on bone destruction, and IL-17 may be actively involved in bone destruction by enhancing RANKL/OPG ratio in CIA model.

Interleukin-17이 배양된 류마티스관절염 활막세포에서 vascular endothelial growth factor 생성에 미치는 영향

곽임수 ( Ihm Soo Kwak ),남태수 ( Tae Soo Nam ),나하연 ( Ha Yeon Rha ),서정탁 ( Jeung Tak Suh ),김유선 ( Yoo Sun Kim ),김성일 ( Sung Il Kim ) 대한류마티스학회 2001 대한류마티스학회지 Vol.8 No.3

Objective: To investigate the the effects of interleukin-17 (IL-17) on the production of vascular endothelial growth factor (VEGF) from cultured rheumatoid arthritis synoviocytes. Methods: Fibroblast-like synovial cells(FLS) were prepared from the synovial tissues of rheumatoid arthritis patients and cultured in the presence of IL-17, IL-17 with or without transforming growth factor-β(TGF-β), tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β). VEGF levels were determined in the culture supernatants by sandwitch ELISA. Results: Stimulation of FLS by serial concentration of IL-17, TGF-β, TNF-α, IL-1β increased the production of VEGF by 2.1-2.7, 2.2-3.0, 2.0-2.9, 2.3-3.1 fold over the constitutive levels of unstimulated FLS. Stimulation of FLS by IL-17 with TGF-β or TNF-α or IL-1β also increased the production of VEGF according to culture periods by 1.6-1.8, 1.1-1.9, 1.5-1.7 fold over the levels stimulated with TGF-β or TNF-α or IL-1β, respectively. This results indicated that IL-17 increased the effect of TGF-β, TNF-α, IL-1β on FLS, leading synergistic enhancement of VEGF production. Conclusion: IL-17 may be involved in the neovascularization in rheumatoid synovitis by enhancing the production of VEGF.

라미네이트 도재와 복합레진 시멘트의 결합강도에 관한 연구

김성일,임호남,박남수,Kim, Sung-Il,Lim, Ho-Nam,Park, Nam-Soo 대한치과보철학회 1991 대한치과보철학회지 Vol.29 No.1

The purpose of this study were to comfirm the effects of the thickness and kinds of porcelain, etchants, illumination time, elapsed time for the measurement, and chemical cure component to the bond strength of porcelain laminate and composite resin cement, and to compare the effects between the light cured resin and the dual cured resins. The etched porcelain surface, the sectioned surface crossing porcelain and resin after bonding, and the debonded surfaces were observed by the SEM. One product of laminate porcelain powder, one light cured resin and two dual cured resins were selected. Each resin cements are lightened through the thin porcelain disc which was cut from cylindrical porcelain specimen by the diamond saw, and by the light through the porcelain disc they were bonded. Changes of thickness and kinds of porcelain, etchants, illumination time, and the elapsed time for the measurement were considered as variables for the bond strength. And the bond strength of porcelain and dual cured resins under the conditions of autopolymerization or the removal of chemical cure component were measured and compared. Bond strength were measured by shear stress. The etched surface, the cross-sectioned surface, and the debonded surface of porcelain or resin were observed by SEM. On the summary of this study, the following conclusions can be stated; 1. Bond strength of light cured resin was decreased inversely by the thickened porcelain laminate and showed the lowest value to the masking dentin porcelain among 4 kinds of porcelain powder. 2. Bond strength of autopolymerization of dual cured resin without illumination in dark chamber were from 75% to 98% to the data of dual cured resin with illumination. 3. Bond strength of dual cured resin used without chemical cured components were same to them of light cured resin. 4. Cross-sectioned surface treated by silane did not show the gap between the porcelain and resin. 5. Illumination over 80 seconds did not make the significant increase of bond strength on all kinds of resin.

건설기술용역 발주 및 입·낙찰 방식의 문제점 및 개선방안

김성일,장철기,박대근,배유진,Kim, Sung-Il,Chang, Chul-Ki,Park, Dae-Keun,Bae, Yu-Jin 한국건설관리학회 2014 한국건설관리학회 논문집 Vol.15 No.5

국내 공공건설사업의 건설기술용역업체 선정을 위한 발주 및 입 낙찰방식을 관련법에서 다양하게 제시하고 있으나, 용역업체 선정을 위한 발주 및 입 낙찰 방식 선정 기준 및 틀이 부재한 실정이다. 아울러, 현행 건설기술 용역업체 선정에서 가격중심의 선정 방식이 주로 활용되고 있다. 이에 본 연구에서는 현행 국내 건설기술용역 발주 및 입 낙찰 방식의 실태조사를 통하여 문제점을 도출하고, 건설기술용역사업의 효과적인 성과 도출과 효율성 극대화를 위해 다양한 발주 및 입 낙찰 방식의 활용방안 및 기존 방식의 개선사항 등을 제시하였다. 이러한 개선방안을 통하여 사업의 성격에 부합한 발주 및 입 낙찰 방식의 활용이 가능한 기반을 조성하고, 기존 가격중심에서 탈피하여 기술기반의 평가요소를 반영하여 용역업체를 선정함으로써, 용역업체의 기술력 향상과 해외시장에서의 경쟁력을 강화하는데 기여 할 수 있을 것으로 판단된다. Various procurement systems for Architecture-Engineering service for public construction project are currently established by construction-related law. However, comprehensive framework and criteria for the selection of Architecture-Engineering firm are in absence. Even though various selection methods for Architecture-Engineering service are currently established by law, cost-based selection method is mainly used in selecting Architecture-Engineering firm for the service. This paper examined current use of procurement system for Architecture-Engineering service and drew problems in practice. Through analysis of problems and bench-marking of those systems in advanced countries and international organizations, several strategies to improve performance of Architecture-Engineering service and to maximize the efficiency were suggested. The suggestions include framework and guideline to select a procurement method for the service which is being considered for the project and way of improving current procurement system. These suggestions will be a foundation for utilizing appropriate procurement system for the specific project and can make domestic Architecture-Engineering firm more competitive in the global market by breaking form the cost-based selection method in the selection of Architecture-Engineering firms.

Cr(III)-picolinate 착물의 합성과 구조분석에 관한 연구

김성일,강대경,차기원,Kim, Sung-Il,Kang, Dae-Kyung,Cha, Ki-Won 한국분석과학회 2003 분석과학 Vol.16 No.1

Cr(III) picolinate는 생체이용성 물질로 동물이나 사람에게 필수 미네랄 크롬을 공급하는 물질로 알려져 있다. 본 연구에서는 두 가지 방법으로 Cr(III)- picolinate를 합성하고 원소분석, FT-IR, 고성능액체크로마토그래피 및 MALDI-MS 등으로 생성물 및 부산물의 순도와 구조를 확인하였다. Chromium(III) picolinate, a highly bioavailable compound, is known to be used for supplementing essential chromium(III) to human beings and animals. Two different methods were used to synthesize chromium(III) picolinate. The synthetic products were characterized by elemental analysis, FTI-IR, high performance liquid chromatography (HPLC) and MALDI-MS.

동안신경마비를 동반한 안와 국균증 1예

김성일,최희영,이지웅,Sung Il Kim,Hee Young Choi,Ji Woong Lee 대한안과학회 2014 대한안과학회지 Vol.55 No.1

Purpose: To report a case of orbital aspergillosis with third nerve palsy. Case summary: A 75-year-old male presented with abrupt onset of visual impairment, extraocular movement limitation, and ptosis. The patient previously experienced rhinolalia and headache and was diagnosed with sinusitis 2 months prior, and was treated with oral antibiotics for 1 week. Orbital magnetic resonance imaging revealed a mass with bone erosion including the nasal cavity, ethmoid bone, and left orbit suggestive of fugal sinusitis. Aspergillus was detected histopathologically in the mass which was removed by endoscopic surgery. Amphotericin B was administered intravenously for 7 days along with voriconazole. There was no recurrence during the follow-up period. Extraocular movement limitations and ptosis were recovered postoperatively. Conclusions: The present study results indicate that visual impairment and third nerve palsy can develop in a patient with orbital aspergillosis. J Korean Ophthalmol Soc 2014;55(1):149-154

화재안전기준(NFSC)에 따른 기계분야 소방설비 설계, 시공, 감리 유지관리 업무 표준화 방안에 대한 제안

김성일,강경식,Kim, Sung-Il,Kang, Kyung-Sik 대한안전경영과학회 2017 대한안전경영과학회지 Vol.19 No.3

The study seeks to cooperate with the investigation of a fire investigation based on public-private partnership. Fire protection systems provide a wide variety of viewpoints from the perspectives of design, construction, management, maintenance, maintenance and maintenance systems at a particular point in time, construction, management, and maintenance systems. What is controversial is that there are illegal activities such as illegal activities of the Patent Office, misconduct of construction work and unreasonable construction of construction works.As a theoretical framework, the present study identified the four key elements of a successful disaster response system : responsiveness, control, professionalism, and bias.

자유기에 의한 지질과산화 반응에 대한 황금 약침액의 항산화 효능

김성일,문진영,김갑성,김두희,남경수,임종국,Kim Sung-Il,Moon Jin-Young,Kim Kap-Sung,Kim Doo-Hie,Nam Kyung-Soo,Lim Jong-Kook 대한예방한의학회 1997 대한예방한의학회지 Vol.1 No.1

Scutellariae radix, has been used as a natural drug for fever, inflammation, cataract, and liver disease in traditional medicine. This study was performed in order to investigate the antioxidative effects of Scutellariae radix aqua-acupuncture solution (SRAS) on lipid peroxidation by free radicals. Lipid peroxidation levels were determined by TBA method during the autoxidation of linoleic acid. In this linoleic acid autoxidation system, SRAS markedly exhibited antioxidant activity, which inhibited 89% of linoleic acid peroxidation. SRAS showed scavenging effects on ${\alpha},{\alpha}-diphenyl-{\beta}-picrylhydrazyl$(DPPH) radical, inhibited superoxide generation in xanthine-xathine oxidase system, and also inhibited lipid peroxidation of rat liver tissue by hydroxyl radical derived from $H_2O_2-FE^{+2}$ system. These effects were similar to those of $dl-{\alpha}-tocopherol$, BHA and BHT. In addition, SRAS protected the cell death induced by ter-butyl hydroperoxide (t-BHP) and significantly increased cell viability in the normal rat liver cell (Ac2F). On the basis of these results, it is suggested that SRAS might play a protective role in lipid peroxidation by free radicals.

전방깊이에 따른 백내장수술 전후의 각막내피세포수의 변화

김성일,나경선,권형구,이현수,김만수,Sung Il Kim,Kyung Sun Na,Hyung Gu Kwon,Hyun Soo Lee,Man Soo Kim 대한안과학회 2010 대한안과학회지 Vol.51 No.12

Purpose: To evaluate the effects of preoperative and postoperative parameters, particularly anterior chamber depth, on corneal endothelial cell loss during cataract surgery. Methods: Eighty-two eyes of 82 patients who underwent cataract surgery by the same surgeon using the same technique were selected for the present study. Various preoperative and postoperative parameters including age, anterior chamber depth, duration and power of phacoemulsification, preoperative endothelial cell density, coefficient of variation, hexagonality, and grade of nucleosclerosis were evaluated. Endothelial cell densities were recorded preoperatively and at two months postoperatively using a noncontact specular microscope. Results: After two months, the mean central endothelial cell loss in all eyes was 8.77%. Among the pre- and post-operative parameters, patient’ age, anterior chamber depth, duration, power, and energy of phacoemulsification and grade of nucleosclerosis showed significant correlation with endothelial cell loss. Stepwise multiple linear regression analysis identified age, nucleosclerosis, anterior chamber depth, and phacoemulsification time as independent predictors for endothelial cell loss. Conclusions: The risk of endothelial cell loss increased with increases in patient’ age, nucleosclerosis, and phacoemulsification time. However, eyes with a deep anterior chamber showed a significantly lower risk of endothelial cell loss. J Korean Ophthalmol Soc 2010;51(12):1568-1572