GST 융합 시스템에서 나타나는 macrophage migration inhibitory factor의 tautomerase 활성 저해에 관한 연구

김상수,김경희,박효진,허은혜,임향숙,Kim Sang-Soo,Kim Kyung-Hee,Park Hyo-Jin,Hur Eun-hye,Rhim Hyangshuk 한국생명과학회 2005 생명과학회지 Vol.15 No.6

Cytokine으로 처음 밝혀진 macrophage migration inhibitory factor (MIF)는 다양한 생물학적 활성 및 효소적 활성을 지닌 단백질로 알려지고 있다. 아직까지 MIF의 서로 다른 활성간의 연관성 및 효소적 기능이 세포내에서 어떤 역할을 하는지는 명확하게 밝혀지지 않았다. 본 연구에서는 MIF가 지닌 효소적 활성 중 tautomerase활성에 대하여 좀더 자세하게 연구하였다. 먼저, GST 융합 시스템을 이용하여 MIF의 정제 조건을 확립하였다. 이를 통해서 얻어진 GST (glutathione S-transferase)-MIF와 MIF 간의 활성을 비교하여 N-말단의 GST에 의하여 MIF의 tautomerase활성이 완전히 저해되는 것을 확인하였다. 다음으로, GST 대신 N-말단에 존재하는 매우 짧은 아미노산 잔기가 MIF의 tautomerase 활성을 저해하는 지를 조사하고자, GST-MIF의 GST를 thrombin으로 제거한 tMIF를 사용하였다. 그 결과 tMIF도 GST-MIF와 마찬가지로 tautomerase 활성이 완전히 저해된다는 사실을 확인할 수 있었다. 추가로 N-말단 tag에 의한 MIF의 효소적 활성 저해가 단백질의 구조적인 변환에 의한 것인지를 조사하기 위하여 단백질 cross-linking 연구를 수행하였다. 단백질 cross-linking 결과 tMIF도 MIF와 같이 정상적으로 oligomer를 형성하는 것을 확인하였다. 이들 결과는 MIF의 N-말단 $P^{2}$를 중심으로 하는 소수성 활성부위의 노출이 tautomerase활성에 결정적인 역할을 한다는 것을 시사한다. 또한 본 연구결과는 세포내에서 MIF가 다른 단백질과의 상호결합에 의해서도 충분히 tautomerase활성이 저해될 수 있을 것이라는 가능성을 제시한다.lelopathy 효과가 있음을 밝혀냈다. 특히 화본과 사료작물의 경우 italian ryegrass, 콩과 사료작물의 경우는 purple alfalfa가 생장억제효과가 높게 나타났다. 영향을 미치지 못했다. 이상의 결과를 종합하면 현재의 체형, 체형에 대한 인식, 매스컴의 영향 등이 체형에 대항 불만족이라는 스트레스로 인지되고, 이러한 이지된 스트레스와 체중조절 행동에 대한 태도가 체중조절 행동의도를 갖도록 하는 것으로 보인다. 특히 다이어트 행동에 대한 태도는 행동결과에 대한 신념이 주요 변수였음을 감안할 때 체형에 대한 불만족을 유발하는 요인들과 감안할 때 체형에 대한 불만족을 유발하는 요인들과 다이어트 행동 결과에 대한 신념이 주요 변수였음을 감안할 때 체형에 대한 불만족을 유발하는 요인들과 다이어트 행동 결과에 대한 신념이 체중조절 행동을 유발하는 주요 요인인 것으로 판단된다. 따라서 과잉 체중조절의 확산을 억제하기 위해서 현재 자신의 체형과 바람직한 체형의 기준에 대한 바른 인식을 갖도록 하는 것이 필요하다. 그리고 체형에 대한 사회적 인식이 중용한 스트레스 요인임을 감안하여 사회 전반에 만연되어 있는 바람직한 체형에 대한 인식의 변화를 위한 노력이 행해져야 한다. 또한 신념이 행동의도에 큰 영향을 미친다는 결과에 따라 다이어트 행동 결과에 대한 신념이 올바른 지식에 근거한 바람직한 신념으로 변화될 수 있도록 하여야 할 것이다. 또한 본 연구에서는 영양지식이 다이어트 행동의도에 아무런 영향을 미치지 않은 것으로 나타났다. 그러나 단기간에 실시한 영양지식의 교육이 행동의 변화까지는 영향을 미치지 않았으나 태도에는 영향을 미친 것을 참고하여 장기적인 목표를 갖고 영양 지식의 보급에도 힘을 기울여야 하겠다.큰 것으로 보인다.의 경우 압흔과 함께 pitting 이 관찰되며, Ormco Stainless Steel의 Macrophage migration inhibitory fartor (MIF), known as a cytokine, is a multifunctional protein that is ubiquitously expressed in a variety of cells and tissues; however, enzymatic function of MIF still remains elusive in cells. In this study, we assessed details of the tautomerase activity of MIF. We established rapid purification condition for MIF by using pGEX system and compared the L-dopachrome tautomerase activity of GST-MIF, tMIF, and MIF. The results show that GST (glutathione S-transferase)-epitope tag or N-terminal amino acids flanking the essential $P^{2}$ almost completely abrogated L-dopachrome tautomerase activity of MIF. Subsequently, to determine whether the N-terminal tags have effects on oligomerization of MIF, protein cross-linking products were analyzed on $15\%$ SDS-PACE. The result demonstrates that N-terminal tags are dispensable for the formation of MIF's homooligomers. Thus, the results imply that exposure of If containing hydrophobic pocket in the active site is critical for L-dopachrome tautomerase activity of MIF. In addition, our study suggest that the MIF's tautomerase activity might be influenced by interacting with cellular partners.

슬링운동과 스위스볼 운동이 체간 유연성 및 근력에 미치는 영향

김상수,권원안,송명수,Kim, Sang-Soo,Kwon, Won-An,Song, Myung-Soo 대한고유수용성신경근촉진법학회 2011 PNF and Movement Vol.9 No.2

Purpose : The Purpose of this study was performed to find out back flexibility and strength by Swiss ball and sling exercise. Methods : Subjects were randomly assigned to 3 groups; Swiss ball(n=9), Sling(n=8) and Swiss ball and Sling(n=8). Each groups had the exercise 30 minute per week for 4 weeks. The case of Swiss ball-sling group had 15 minutes for ball exercise and 15 minute for sling exercise. Results : First, sling group among groups significantly increased in flexibility test 1. Second, ball group among groups significantly increased in flexibility test 2. Third, ball-sling group among groups significantly increased in strength test 1 and 2. Fourth, follow-up study after 2 weeks was revealed that flexibility and strength were decreased in all groups. Conclusion : The effect were not consistent with exercise methods. Accordingly, it should be selected for purpose and effect you want.

물리치료과 학생들의 임상실습만족도에 관한 연구 : 일부지역을 중심으로

김상수,서현규,김병곤,이재홍,권원안,Kim, Sang-Soo,Seo, Hyun-Kyu,Kim, Byoung-Gon,Lee, Jae-Hong,Kwon, Won-An 대한고유수용성신경근촉진법학회 2012 PNF and Movement Vol.10 No.1

Purpose : This study was investigation of satisfaction in clinical practice of students in a college. Methods : 250 students were participated in this study for 8 weeks though getting results of clinical practice from 1, 2, 3 clinic hospitals. Results : This was for knowing about achievement, It could figure out the increasing achievement the higher satisfaction with education and environment for clinical practice of physical therapy. As a result, whole satisfactions for environment and course or education in clinical practice was higher. Conclusion : This research was not too much higher satisfaction on participated students in clinical practice. So, it needs to suggest to more study for increasing satisfaction on clinical practice.

중년 남환에서 다병소성(多病巢性) 골수염으로 오진된 Langerhans Cell Histiocytosis (LCH)의 치험례

김상수,박형식,윤현중,허진영,김진권,정재호,Kim, Sang-Soo,Park, Hyung-Sik,Yong, Hyun-Jung,Huh, Jin-Young,Kim, Jin-Kwon,Jung, Jae-Ho 대한악안면성형재건외과학회 1997 Maxillofacial Plastic Reconstructive Surgery Vol.19 No.1

Langerhans Cell Histiocytosis(Idiopathic Histiocytosis, Histiocytosis-X) is most often found in children and young adults, and cell proliferation with specific phenotype shows ultrastructural and immunohistochemical similarities with Langerhans Cells that normally exist in epithelium and mucosa. This disease occurs as single or multiple lesions in skull, ribs, vertebrae, mandible and long bones, and when it involves mandible, clinical sign and symptoms such as bone swelling and pain are noticed. When it involves alveolar bone, severe tooth mobility as well as gigival inflammation, proliferation, and ulceration are commonly found, and so it is not easy to differentiate it from general inflammatory diseases. Any local lesion at the tooth apex on the x-ray view needs to be differentiated from inflammatory disease, and multiple lesions from multiple ostoeoma and chronic multifocal osteomyelitis. This case is LCH in 51-year-old male patient ; this is a rare case, for the patient belongs to an age group with very low incidence rate of the disease. although three-timed biopsy tests and longterm observation at two university hospitals, it was misdiagnosed as multifocal osteomyelitis.

사회적지지가 베이비부머의 창업기회역량에 미치는 영향에 관한 연구: 창업자기효능감의 매개효과를 중심으로

김상수,임왕규,Kim, Sang Soo,Lim, Wang Kyu 한국벤처창업학회 2017 벤처창업연구 Vol.12 No.5

본 연구는 베이비부머 (1955-1963년생)를 대상으로 창업자기효능감을 매개 변수로 하여 사회적지지가 창업기회역량에 미치는 영향에 관하여 분석하였다. 창업정책과 교육은 청년창업사관학교 등의 제도를 통해 대부분 대학생 및 청장년층을 위주로 진행되고 있고, 또 이들을 대상으로 사회적지지나 제도적지원이 창업의지에 미치는 영향 등에 대한 연구는 활발히 진행되고 있는 반면, 베이비부머에 대한 연구는 복지부문에 치우치고 있고 실제로 이들은 은퇴 후 생계를 위해 치밀한 준비없이 생계형 창업에 뛰어들고 있으나, 이 베이비부머를 대상으로 한 사회적지지가 기회형 창업 추진에 필요한 창업기회역량에 미치는 영향에 관한 연구는 거의 없다시피 하다. 이에 본 연구는 사회적지지가 베이비부머의 창업기회역량에 미치는 영향을 알아보기 위해 주로 서울시와 경기도 일원에 거주하는 베이비붐 세대들로부터 수집한 설문지 387부를 실증 분석을 실시하였다. 분석 결과 첫째, 사회적지지가 창업기회역량의 기회인식과 기회평가 모두에 유의한 영향을 미치는 것으로 나타났다. 둘째, 사회적지지가 창업자기효능감에 미치는 영향에서는 정서적, 물질적 지지만 자신감에 유의한 영향을 미치고, 정서적지지와 평가적지지만 자기조절효능감에 유의한 영향을 미치고, 정서적지지와 물질적지지만이 과업도전감에 유의한 영향을 미치는 것으로 나타났다. 셋째, 창업자기효능감 중 자기조절효능감과 과업도전감 만이 창업기회인식에 유의한 영향을 미치는 것으로 나타났고, 자신감, 자기조절효능감 및 과업도전감 모두가 기회평가에 유의한 영향을 미치는 것으로 나타났다. 넷째, 사회적지지가 창업자기효능감을 매개하여 창업기회역량에 미치는 간접효과는 부분적으로 유의한 것으로 나타났다. This study analyzed the effects of social support on entrepreneurial opportunity competence for Baby boomer (born in 1955-1963), focusing on mediating effect of entrepreneurial self-efficacy. The governmental start-up policy and entrepreneurship education are mostly conducted for the college students and young generation mainly through such programs as the youth start-up school. In addition, studies on the effects of social support and institutional support on the entrepreneurial intention of young generation entrepreneurs are being actively pursued. Meanwhile, the study for baby boomers is on the welfare side mainly. Although they are starting to make their living-based business without prior careful preparation for their livelihood after retirement, while there is little study on the effect of social support for this baby boomer on entrepreneurial opportunity competence needed for opportunistic entrepreneurship. Therefore, in this study, in order to investigate the effect of social support on Baby boomer's entrepreneurial opportunity competence, an empirical analysis of the 387 questionnaires collected by the baby boomers living in Seoul and Gyeonggi provinces were conducted. As a result of analysis, First, Social support has a significant effect on both Opportunity recognition and Opportunity assessment of entrepreneurial opportunity competence. Second, In the effects of social support on self-efficacy were found to be significant in Emotional and Instrument support only on Self-confidence, In Emotional support and Feedback support only on Self-regulated efficacy & Emotional support and Instrument support only on Goal challenge were found to be a significant effect. Third, Self-regulated efficacy and Goal challenge in the Entrepreneurial self-efficacy on Entrepreneurial opportunity recognition were found to be a significant effect. All Self-confidence, Self-regulated efficacy & Goal challenge in the Entrepreneurial self-efficacy on Entrepreneurial opportunity assessment were found to be a significant effect. Fourth, Indirect effect of social support on entrepreneurial opportunity competence mediated by Entrepreneurial self-efficacy was found to be partially significant.

항공교통관제사의 감정통제가 직무몰입과 조직충성도에 미치는 영향

김상수,김기웅,최진영,이명우,최연철,Kim, Sang Soo,Kim, Kee Woong,Choi, Jin Young,Lee, Myung Woo,Choi, Youn Chul 한국항공운항학회 2016 한국항공운항학회지 Vol.24 No.4

Air Traffic Controllers (ATC) are also essential to the efficiency of airports and the airline industry. With the increasing volume of traffic, managing flights is a complex and sometimes very stressful job. ATC is using radar and other technology to track planes 'en route' between airports and keep in touch with pilots. Alternatively, ATCs are liaising with the planes on approach. However, ATC's working condition is getting more stressful, as the volume of air traffic increases. Thus this paper tried to research cognitive emotional regulation, job satisfaction, and job involvement and organizational committment of ATCs under the stressful work condition, taking care of safety of a couple of hundreds passengers per plane. Using CERQ survey sheet (Cognitive Emotional Regulation Questionnaire), it was found out that positive thinking and acceptance of self-blame have a significant impact on job satisfaction, work absorption and organizational Loyalty.

폐결핵(肺結核) 환자(患者)에서 수소(愁訴)가 치료과정(治療過程)에 미치는 영향(影響)

김상수,Kim, Sang-Soo 대한예방의학회 1982 예방의학회지 Vol.15 No.1

In order to investigate the relation of subjective complaints to objective course of treatment in pulmonary tuberculosis, one hundred and seventy four cases registered at the University Health Center in Chonnam University were divided into two groups, complaint group and non-complaint group. By analysing the record at the University Health Center during these seven years from 1973 to 1979, following results were obtained: 1. In complaint group who had subjective symtoms, thirty eight cases(77.6%) among forty nine cases were detected. at clinic visit. In non-complaint group who had not any symtoms, one hundred and twenty one cases (96.8%) among one hundred and twenty five cases were detected at compulsive physical examination. 2. Comparisons of complaint group with non-complaint group were not statistically significant in many aspects, but significantly higher cases were discharged at the University Health Center to receive other medical service in complaint group than in non-complaint group. (6.1% vs 0.8%, respectively) On the contrary, non-complaint group refused medication more than two times compared with complaint group in the course of treatment (29.9% vs 12.2%, respectively). 3. In the average time lag from initial diagnosis to initial treatment, comparisons between complaint vs non-complaint group were significant (7.8 vs 28.3 days, respectively). 4. Subjective symptoms were thought to create motivation to receive therapy, and influence of motivation to therapy was strong at initial stage but it become weaker as time passed.

체간신전근의 근력강화운동과 신장운동이 20대 청소년의 근력 및 유연성에 미치는 영향

김상수,이우형,송명수,Kim, Sang-Soo,Lee, Woo-Hyung,Song, Myung-Soo 대한고유수용성신경근촉진법학회 2010 PNF and Movement Vol.8 No.1

Purpose : This study aimed to examine the Influence of muscle strengthening exercise and stretching exercise of trunk extensor muscles on muscular strength and flexibility in adolescent of the twenties. Methods : Muscle strengthening exercise group(n=10), stretching exercise group(n=10) and strengthening and stretching exercise group(n=10) were measured muscular strength by digital back and leg dynamometer and flexibility by sit and reach test and trunk extension test at pre-intervention and post-intervention in 4weeks. Results : The flexibility 1, flexibility 2 and muscular strength of muscle strengthening exercise group were significantly increased within the intervention period(p<.05). The flexibility 2 and muscular strength of stretching exercise group were significantly increased within the intervention period(p<.05). but flexibility 1 was not significantly different at in 4 weeks(p>.05). The flexibility 1, flexibility 2 and muscular strength of muscle strengthening and stretching exercise group were significantly increased within the intervention period(p<.05). Conclusion : We were found that muscle strengthening exercise and stretching exercise of trunk extensor muscles could improve muscular strength and flexibility for adolescent of the twenties.

하악골운동 추적을 위한 단순기록장치 고안에 관한 연구

김상수,윤창근,Kim, Sang-Soo,Yoon, Chang-Keun 대한치과보철학회 1987 대한치과보철학회지 Vol.25 No.1

The purpose of this study was to improve limitations and disadvantages of the mechanical pantograph and the Visi-Trainer, and to design the reliable and reproducible device mandibular movement tracking device (MMTD) that is more simple, convenient and save the chair time than the mechanical pantograph and Visi-Trainer. MMTD was consist of head frame, horizontal bar, condylar path tracking stylus holders, anterior path tracking stylus holder, two condylar path plastic recording plates, one anterior path plastic recording plate, toggles and open occlusal clutch. To prove the reliability and reproducibility of MMTD, a five adults were selected and mandibular condylar movement was recorded one time by the mechanical pantographic tracing and MMTD. The border movement recording of the mandibular incisor (frontal, sagittal and horizontal) was also recorded by Visi-Trainer and the MMTD. The obtained results were as follows; 1. The condylar movement path (sagittal, horizontal) of the MMTD was not coincidence with that of mechanical pantograph. 2. Measurements of the angulation which established between working and balancing path records by use of the mechanical pantograph and MMTD, there was no significance. 3. In a view of MMTD's reproducibility, there was revealed almost similar recording pattern. 4. The border movement recording of the mandibular incisor by the MMTD and the Visi-Trainer showed almost same reliability and reproducibility. 5. The subjects were able to his original mandibular movements by use of open occlusal clutch in the MMTD.

감마선이 조사된 미성숙 생쥐 난소 내 인히빈의 발현 변화

김상수,이창주,윤현태,윤용달,Kim, Sang Soo,Lee, Chang Joo,Yoon, Hyun-Tae,Yoon, Yong-Dal 대한생식의학회 2006 Clinical and Experimental Reproductive Medicine Vol.33 No.1

목 적: 본 연구의 목적은 난소 내 인히빈-${\alpha}$에 단백질 발현과 인히빈 ${\alpha}$, ${\beta}A$, 그리고 ${\beta}B$ 유전자의 발현에 감마선이 미치는 효과를 연구하는 것이다. 연구방법: 미성숙 생쥐에 감마선을 치사량의 25% 효과로 전신 조사하였다. 감마선 조사 후, 0, 3, 6, 12, 24시간이 지나서 난소를 적출하였다. 적출한 난소를 이용하여, 인히빈 ${\alpha}$에 대한 면역조직화학 염색과 인히빈 ${\alpha}$, ${\beta}A$, 그리고 ${\beta}B$에 대한 RT-PCR을 수행하였다. 결 과: 인히빈 ${\alpha}$ 면역양성반응성은 12시간 방사선 조사 후까지 유지가 되었고, 이후에 감소하였다. 인히빈 ${\alpha}$ mRNA의 발현은 방사선 처리 후에 유의하게 증가하였다. 그러나, 인히빈 ${\beta}A$와 ${\beta}B$의 mRNA의 발현은 유의한 변화가 나타나지 않았다. 결 론: 인히빈은 감마선 조사로 유도된 생쥐난포 폐쇄에 조절적 요소로 작용하는 것으로 사료된다. Objective: The purposes of the present study were to investigate the effect of ${\gamma}$-radiation on the expression of inhibin-${\alpha}$ proteins and genes for inhibin ${\alpha}$, ${\beta}A$, and ${\beta}B$ in the ovary. Methods: Immature mice were whole-body ${\gamma}$-irradiated with 25% of a lethal dose. At time 0, 3, 6, 12, and 24 hours after the irradiation, the ovaries were collected and used for immunohistochemistry for inhibin-${\alpha}$, and RT_PCR for inhibin-${\alpha}$, ${\beta}A$, and ${\beta}B$. Results: The expression of the immunoreactive inhibins-${\alpha}$ was maintained at 12 hours post-irradiation and reduced thereafter. The expression of inhibin-${\alpha}$ mRNA was significantly increased with the time after the irradiation. However there were no significant changes in the expression of ${\beta}A$ and ${\beta}B$ mRNAs. Conclusion: It might be thought that inhibin acts as one of the regulatory factors in the ${\gamma}$-radiation-induced follicular atresia in mice