Molecular Characterization of Extracellular Medium-chain-lengthPoly(3-hydroxyalkanoate) Depolymerase Genes fromPseudomonas alcaligenes Strains

김도영,Hyun Chul Kim,Sun Young Kim,이영하 한국미생물학회 2005 The journal of microbiology Vol.43 No.3

A bacterial strain M4-7 capable of degrading various polyesters, such as poly(ε-caprolactone), poly(3-hydroxybutyrate-co-3-hydroxyvalerate),poly(3hydroxyoctanoate), and poly(3-hydroxy-5-phenylvalerate),was isolated from a marine environment and identified as Pseudomonas alcaligenes. The relative molecular mass of a purified extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA)depolymerase (PhaZPalM4-7) from P. alcaligenes M4-7 was 28.0 kDa, as determined by SDS-PAGE. The PhaZPalM4-7 was most active in 50 mM glycine-NaOH buffer (pH 9.0) at 35oC. It was insensitive to dithiothreitol, sodium azide, and iodoacetamide, but susceptible to p-hydroxymercuribenzoic acid,bromosuccinimide, acetic anhydride, EDTA, diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride,Tween 80, and Triton X-100. In this study, the genes encoding MCL-PHA depolymerase were cloned,sequenced, and characterized from a soil bacterium, P. alcaligenes LB19 (Kim et al., 2002, Biomacromolecules 3, 291-296) as well as P. alcaligenes M4-7. The structural gene (phaZPalLB19) of MCL-PHA depolymerase of P. alcaligenes LB19 consisted of an 837 bp open reading frame (ORF) encoding a protein of 278 amino acids with a deduced Mr of 30,188 Da. However, the MCL-PHA depolymerase gene (phaZPalM4-7) of P. alcaligenes M4-7 was composed of an 834 bp ORF encoding a protein of 277 amino acids with a deduced Mr of 30,323 Da. Amino acid sequence analyses showed that, in the two different polypeptides, a substrate-binding domain and a catalytic domain are located in the N-terminus and in the C-erminus,respectively. The PhaZPalLB19 and the PhaZPalM4-7 commonly share the lipase box, GISSG,in their catalytic domains, and utilize 111Asn and 110Ser residues, respectively, as oxyanions that play an important role in transition-state stabilization of hydrolytic reactions. A bacterial strain M4-7 capable of degrading various polyesters, such as poly (ε-caprolactone), poly(3-hydroxybutyrate-co-3-hydroxyvalerate),poly(3hydroxyoctanoate), and poly(3-hydroxy-5-phenylvalerate), was isolated from a marine environment and identified as Pseudomonas alcaligenes. The relative molecular mass of a purified extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA)depolymerase (PhaZPalM4-7) from P. alcaligenes M4-7 was 28.0 kDa, as determined by SDS-PAGE. The PhaZPalM4-7 was most active in 50 mM glycine-NaOH buffer (pH 9.0) at 35oC. It was insensitive to dithiothreitol, sodium azide, and iodoacetamide, but susceptible to p-hydroxymercuribenzoic acid,bromosuccinimide, acetic anhydride, EDTA, diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride,Tween 80, and Triton X-100. In this study, the genes encoding MCL-PHA depolymerase were cloned,sequenced, and characterized from a soil bacterium, P. alcaligenes LB19 (Kim et al., 2002, Biomacromolecules 3, 291-296) as well as P. alcaligenes M4-7. The structural gene (phaZPalLB19) of MCL-PHA depolymerase of P. alcaligenes LB19 consisted of an 837 bp open reading frame (ORF) encoding a protein of 278 amino acids with a deduced Mr of 30,188 Da. However, the MCL-PHA depolymerase gene (phaZPalM4-7) of P. alcaligenes M4-7 was composed of an 834 bp ORF encoding a protein of 277 amino acids with a deduced Mr of 30,323 Da. Amino acid sequence analyses showed that, in the two different polypeptides, a substrate-binding domain and a catalytic domain are located in the N-terminus and in the C-erminus,respectively. The PhaZPalLB19 and the PhaZPalM4-7 commonly share the lipase box, GISSG, in their catalytic domains, and utilize 111Asn and 110Ser residues, respectively, as oxyanions that play an important role in transition-state stabilization of hydrolytic reactions.

광검출기 응용을 위하여 스퍼터된 미세결정 SiGe 박막성장 연구

김도영,김선조,김형준,한상윤,송준호,Kim, Do-Young,Kim, Sun-Jo,Kim, Hyung-Jun,Han, Sang-Youn,Song, Jun-Ho 한국전기전자재료학회 2012 전기전자재료학회논문지 Vol.25 No.6

For the application of photo-detector as active layer, we have studied how to deposit SiGe thin film using an independent Si target and Ge target, respectively. Both targets were synthesized by purity of 99.999%. Plasma generators were generated by radio frequency (rf, 13.56 MHz) and direct current (dc) power. When Ge and Si targets were sputtered by dc and rf power, respectively, we could observe the growth of highly crystalline Ge thin film at the temperature of $400^{\circ}C$ from the result of raman spectroscopy and X-ray diffraction method. However, SiGe thin film did not deposit above method. Inversely, we changed target position like that Ge and Si targets were sputtered by rf and dc power, respectively. Although Ge crystalline growth without Si target sputtering deteriorated considerably, the growth of SiGe thin film was observed with increase of Si dc power. SiGe thin film was evaluated as microcrystalline phase which included (111) and (220) plane by X-ray diffraction method.

Antiglide 금속판을 이용한 족관절의 외과 골절의 치료

김도영,신주호,조원호,황현철,Kim, Do-Young,Shin, Joo-Ho,Cho, Won-Ho,Hwang, Hyun-Chull 대한족부족관절학회 2001 대한족부족관절학회지 Vol.5 No.1

Most of Danis-Weber type B fracture has an oblique fracture plane proximal posteriorly to distal anteriorly. In these cases, the lateral plate has become widely accepted. However some limitation to the use of the lateral plate exist. The plate must be bent accurately and the screws in the distal fragment must be unicortical to avoid penetration into the joint, predisposing it to poor fixation in the osteoporotic bone. In 1982, Brunner and Weber introduced the antiglide plate for fixation of the shaft oblique fracture of the distal fibula, but it has not widely used. The author reviewed 21 cases treated by antiglide plate fixation from March, 1995 to March, 1999 which could be follow-up more than 1 year. We analysed the result radiographically and clinically using Ankle-Hindfoot scale(100 % total) of the American Orthopedic Foot and Ankle Society. The results obrained were as follows: 1. All fracture were united at average 8 weeks clinically and radiographically. 2. According to the Ankle- Hindfoot scale, 8 cases were above 90 points, 11 cases were beet ween 80 and 89 points and two cases were below 80 points. 3. One case had an injury to intermediate dorsal cutaneous nerve.

The Formation of Microcrystalline SiGe Film Using a Remote Plasma Enhanced Chemical Vapor Deposition

김도영,Kim, Doyoung The Korean Institute of Electrical and Electronic 2018 전기전자재료학회논문지 Vol.31 No.5

SiGe thin films were deposited by remote plasma enhanced chemical vapor deposition (RPE-CVD) at $400^{\circ}C$ using $SiH_4$ or $SiCl_4$ and $GeCl_4$ as the source of Si and Ge, respectively. The growth rate and the degree of crystallinity of the fabricated films were characterized by scanning electron microscopy and Raman analysis, respectively. The optical and electrical properties of SiGe films fabricated using $SiCl_4$ and $SiH_4$ source were comparatively studied. SiGe films deposited using $SiCl_4$ source showed a lower growth rate and higher crystallinity than those deposited using $SiH_4$ source. Ultraviolet and visible spectroscopy measurement showed that the optical band gap of SiGe is in the range of 0.88~1.22 eV.

원자층 증착법으로 성장된 ZnO 박막의 질소 도핑에 대한 연구

김도영,Kim, Doyoung 한국전기전자재료학회 2014 전기전자재료학회논문지 Vol.27 No.10

For feasible study of opto-electrical application regarding to oxide semiconductor, we implemented the N doped ZnO growth using a atomic layer deposition technique. The p-type ZnO deposition, necessary for ZnO-based optoelectronics, has considered to be very difficulty due to sufficiently deep acceptor location and self-compensating process on doping. Various sources of N such as $N_2$, $NH_3$, NO, and $NO_2$ and deposition techniques have been used to fabricate p-type ZnO. Hall measurement showed that p-type ZnO was prepared in condition with low deposition temperature and dopant concentration. From the evaluation of photoluminescence spectroscopy, we could observe defect formation formed by N dopant. In this paper, we exhibited the electrical and optical properties of N-doped ZnO thin films grown by atomic layer deposition with $NH_3OH$ doping source.

프로토타이핑 기법에 의한 키네틱 외피의 설계: 디지털-아날로그 모델의 상호작용과 BIM의 역할을 중심으로

김도영,김성아,Kim, Do-Young,Kim, Sung-Ah 한국BIM학회 2015 KIBIM Magazine Vol.5 No.1

The kinetic facade system is an interactive building envelope which is adaptive to environmental condition by transforming the behaviour of its components. The design process of kinetic facade system calls for a novel approach. It needs to support designers to adopt technologies from multidisciplinary fields such as physical computing and robotics. In this paper, prototyping method is introduced as a useful technique for implementing kinetic facade systems. In order to incorporate prototyping method into architectural design process, two aspects are investigated in digital design studio: (1) The interactions between digital and analogue environments (2) The role of traditional design tools. Furthermore, the role of BIM is investigated by analyzing two aspects.

치조정 골흡수에 대한 platform switching이 미치는 영향

김도영,김태일,설양조,이용무,구영,류인철,정종평,한수부,Kim, Do-Young,Kim, Tae-Il,Seol, Yang-Jo,Lee, Yong-Moo,Ku, Young,Rhyu, In-Chul,Chung, Chong-Pyoung,Han, Soo-Boo 대한치주과학회 2008 Journal of Periodontal & Implant Science Vol.38 No.2

Purpose: Numerous studies have shown that crestal bone resorption around the implant was related to the location of the implant abutment junction(IAJ). Recently it was hypothesized that platform switching termed the inward horizontal repositioning of the IAJ might limit bone resorption around the implants. The purpose of this clinical study was to evaluate the effect of platform switching on crestal bone resorption. Materials and Methods: The crestal bone loss of 65 external hex implants in 26 patients were radiographically measured at crown placement and follow-up examinations. 23 standard implants(non-platform switching group, NP) were connected with the matching abutments and 42 wide implants(platform switching group, PS) were connected with the 1 mm smaller diameter abutments. Results: There was significant difference of crestal bone loss between NP group and PS group. For implants in the NP group, mean crestal bone loss was $1.18{\pm}0.68\;mm$ at crown placement and $1.42{\pm}0.41\;mm$ at follow-up. The meal bone loss in PS group was $0.47{\pm}0.52\;mm$ at crown placement and $0.60{\pm}0.65\;mm$ at follow-up. When the crestal bone changes according to placement depths of implants were compared, subcrestal position of IAJ had a significantly less bone loss in PS group, but it was not in NP group. Conclusion: Within the limits of the present study, it was concluded that platform switching technique might decrease crestal bone loss around the implants. Additionally, when the IAJ of implant was placed 1 mm deeper in the alveolar bone, the effect of platform switching on bone loss was enhanced.

Titanium 표면처리 방법이 Osteoblast-like Cells의부착 및 증식에 미치는 영향

김도영,설양조,류인철,함병도,정종평,최상묵,김우진,백홍구,허성주,한종현,김명호,최용창,전흥재,권수경,Kim, Do-Yung,Seol, Yang-Jo,Rhyu, In-Cheul,Hahm, Byung-Do,Chung, Chong-Pyoung,Choi, Sang-Mook,Kim, Woo-Jin,Baik, Hong-Koo,Heo, Seong-Joo,Han, Cho 대한치주과학회 2000 Journal of Periodontal & Implant Science Vol.30 No.3

In clinical therapy, the current goal of dental implants is to enhance quantity and quality of osseointegration. Surface roughness and oxide structure are considered to influence the behavior of adherent cells. The purpose of this study is to evaluate the effect of different surface treatment on cellular response. The attachment and proliferation of osteoblast-like cell on sandblasted, sandblasted and etched, thermal oxidated surfaces have been compared. Sandblasting was done with $Al_2O_3$ particles(grain size of $50{\mu}m$), etching was processed with $NH_4OH$ : $H_2O_2$ : $H_2O(1:1:5)$ at $90^{\circ}C$ for 1 minute. Thermal oxidation was followed sandblasting and etching at $400^{\circ}C$, $600^{\circ}C$, $800^{\circ}C$ for 2 hours. Measurement of surface roughness after the different treatment did not show any differences of Ra value between terated surfaces. Cell attachment and proliferation were increased during experiment period, but no difference was observed. SEM evaluation revealed a similar pattern of osteoblast-like cells, well attached with dendritic extension and producing numerous matrix vesicles on cell surface. The results of this study showed that oxide layer alteration by thermal oxidation did not affect the attachment and proliferation of osteoblast-like cells. This suggests the possibility that the cellular responses are further influenced by surface roughness than titaniun oxide structure.

회피행동 분석을 이용한 서울시 수돗물 수질개선의 편익추정

김도영,김경환,Kim, Do-Yeong,Kim, Gyeong-Hwan 한국자원경제학회 1994 資源經濟學會誌 Vol.3 No.2

Suture anchor를 이용한 외측 반월판 연골 전방 뿌리 파열의 관절경적 치료 -증례 및 술기 보고-

김도영,유연식,이상수,정운섭,서은민,박승재,Kim, Do-Young,Yoo, Yon-Sik,Lee, Sang-Soo,Jeong, Un-Seob,Seo, Eun-Min,Park, Seung-Jae 대한정형외과스포츠의학회 2011 대한정형외과스포츠의학회지 Vol.10 No.1

반월상 연골판의 뿌리파열의 심각성이 인식되면서 이에 대한 적극적인 봉합이 필요하게 되었다. 특히 후각부 뿌리 파열에 대한 봉합 방법과 결과가 최근에 많이 보고되고 있다. 그러나 전각부 뿌리 파열에 대한보고는 아직 보고된 바 없으며 이에 저자들은 과거에 시행했던 원판형 연골의 부분 절제 후 발생한 의인성 뿌리 파열과 이로 인한 아탈구를 경험하였고 이를 봉합나사를 이용하여 정복하여 좋은 결과를 얻었기에 보고하는 바이다. As awareness of root tear of meniscus have been increased, it became necessary to have an intensive repair. Posterior horn tear, especially for a lot of repair methods and the results have been reported recently. The report of the anterior root tear has yet to be found. We recently encountered a patient with an iatrogenic meniscal subluxation caused by past experienced partial menisectomy for discoid meniscus. Thus, we repots the technique and clinical results of arthroscopic repair of subluxated lateral meniscus anterior root tear using suture anchor.