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강호범,류승희,이상훈,전병순,상병찬 충남대학교 농업과학연구소 1998 농업과학연구 Vol.25 No.1
This study was performed in order to apply to effective breeding of Korean native cattle on the molecular genetic level obtained from PCR and nucleotide sequencing analysis of BoLA DRB3 exon2 that has important roles in host immune defence. Genomic DNA used in this study was prepared from the blood of Korean native cattle in Korean Native Cattle Improvement Center of National Livestock Cooperation. The results obtained from this study are summarized as follows: 1. Genomic DNA extracted from the blood of Korean native cattle was subjected to electrophoresis on 1.5% agarose gel. Major band was bigger than 12.2kb, indicating that genomic DNA was well prepared for PCR. Amplified products of 284bp fragments was obtained the amplification of BoLA DRB3 exon2 gene by PCR. 2. Cloning of BoLA DRB3 exon2 of Korean native cattle with pCR2.1 vector was conformed by 300bp fragment from recombinent plasmid that restricted with enzyme digestion of EcoRI. 3. Homology of BoLA DRB3 exon2 alleles of parent was 82.0% between sire's alleles and 90.1% between dam's alleles. 4. In pedigree analysis using BoLA DRB3 exon2 gene, sequencing result of BoLA DRB3 exon2 genes showed inheritance by Mendelian mode through the parents to their offspring. 5. Taking together those experimental results, pedigree was confirmed on the basis of sequencing for the alleles of parents and offspring. This knowledge by the molecular biological approach could be served for the improvement of Korean native cattle.
강호범 ( Ho Bum Kang ),김영은 ( Young Eun Kim ),이영희 ( Young Hee Lee ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4
Innate immunity represents the inherited resistance to microbial infection. Molecular mechanism in the antimicrobial host defense of the embryo has not yet been studied. This study was designed to investigate whether genes of antimicrobial peptides and antiviral molecules are expressed in human embryonic stem(hES) cells. We checked expression of genes for defensin family peptides, S100 family proteins and antiviral response molecules by RT-PCR in two hES cell lines MizhES4 and SNUhES3. We found expression of antimicrobial peptides α-defensin 6(DEFA6), β-defensin 1(HBD1) and β-defensin 2(HBD2). Human ES cells expressed genes for antimicrobial S100 calcium-binding proteins such as S100A7, S100A8 and S100A9. Expression of antiviral response related genes such as interferon-αand MxA was also detected. Taken together, our results reveal that hES cells express antimicrobial and antiviral molecules and suggest a possibility that these molecules also contribute to self-defense at the early embryonic stage.
PMA로 자극되어진 세포에서 염증 Cytokine 발현에 미치는 Bovine Lactoferrin의 생물활성 영향
정승희,강호범,김재화,윤성식,남명수,Chung, Sung-Hee,Kang, Ho-Bum,Kim, Jae-Wha,Yoon, Sung-Sik,Nam, Myoung-Soo 한국축산식품학회 2012 한국축산식품학회지 Vol.32 No.3
유청단백질의 일종인 lactoferrin은 이미 많은 보고에 의해서 여러 가지 생리활성기능이 있는 것으로 밝혀지고 있는데, U937, NK92, 수지상세포의 분화된 상태인 mutz-3와 muDC를 이용하여 과민반응과 천식, 면역 관련 유전자의 발현에 미치는 영향을 조사 한 결과 의미 있는 결과를 도출하였다. Lactoferrin 단독 또는 면역증강물질인 PMA와 혼합하여 처리 한 경우 상승효과 작용으로 과민반응과 천식, 면역 관련 유전자의 발현을 강하게 유도하였다. 이는 유청단백질의 주요 성분 중 하나인 lactoferrin이 면역기전에 중요한 역할을 하고 있다는 결과로 사료된다. Bovine lactoferrin is well known as biological activator in defense mechanism related some cells. In this study, we was investigated about the immune modulator as a role of lactoferrin through the transcriptional regulation of genes associated with hypersensitivity such as allergy, athma and inflammatory disease. Effects of inflammatory reaction of bovine lactoferrin was carried out by RT-PCR analysis from isolated total RNA treated with lactoferrin 0, 10, 50, 100, 500 ${\mu}g/mL$ and PMA 100 ng/mL. The expression of the TYROBP, PITPNA, IL-10, SLP1, DC-stamp and ICAM-1 mRNA were increased by synergy effect of bovine lactoferrin and PMA. The results of RT-PCR showed that bovine lactoferrin and PMA had an effect of immune modulator by enhancement of TYROBP, PITPNA, SLP1, DC-stamp, IL-10 and ICAM-1 gene transcription in U937, Mutz-3 and NK92 cells, respectively. Bovine lactoferrin showed a potential of biological function which could be used for industrial applications as a material of food and pharmaceutical.
강상욱,박승범,강경훈,강호갑,이규정 한국경영정보학회 2007 한국경영정보학회 학술대회논문집 Vol.2007 No.-
본 연구는 기술적인 측면에서 불법 콘텐츠 유통을 방지하기 위한 현행 추적기술들을 조사해 보고, 이들이 갖고 있는 문제점을 살펴본 후 디지털콘텐츠 식별체계 국가표준으로 채택된 한국정보사회진흥원의 UCI(Universal/Ubiquitous Content Identifier) 시스템과의 연계를 통해 문제점을 해결할 수 있는 방안을 연구하여 불법콘텐츠 사용방지를 위한 기술적, 정책적 방안을 도출하며, 결론으로서, 이러한 불법콘텐츠 추적기술들과 UCI와의 연계를 통해 추적시스템에서의 CP 정보 중복 저장 방지 및 관리의 편리성을 향상시킬 수 있는 방안을 제시할 것이다.
윤선영,강호범,고영은,신수현,김영준,손기영,한용해,정새호,김재화 대한면역학회 2015 Immune Network Vol.15 No.2
Controlling balance between T-helper type 1 (Th1) and T-helper type 2 (Th2) plays a pivotal role in maintaining the biological rhythm of Th1/Th2 and circumventing diseases caused by Th1/Th2 imbalance. Interleukin 4 (IL-4) is a Th2-type cytokine and often associated with hypersensitivity- related diseases such as atopic dermatitis and allergies when overexpressed. In this study, we have tried to elucidate the function of 1-palmitoyl-2-linoleoyl-3-acetyl- rac-glycerol (EC-18) as an essential modulator of Th1/ Th2 balance. EC-18 has showed an inhibitory effect on the production of IL-4 in a dose-dependent manner. RT-PCR analysis has proved EC-18 affect the transcription of IL-4. By analyzing the phosphorylation status of Signal transducer and activator of transcription 6 (STAT6), which is a transcriptional activator of IL-4 expression, we discovered that EC-18 induced the decrease of STAT6 activity in several stimulated cell lines, which was also showed in STAT6 reporter analysis. Co-treatment of EC-18 significantly weakened atopy-like phenotypes in mice treated with an allergen. Collectively, our results suggest that EC-18 is a potent Th2 modulating factor by regulating the transcription of IL-4 via STAT6 modulation, and could be developed for immune-modulatory therapeutics.
Different Potential of Hematopoietic Differentiation in Two Distinct Mouse Embryonic Stem Cells
김진숙,강호범,송지연,오구택,남기환,이영희 한국발생생물학회 2005 발생과 생식 Vol.9 No.2
배아 줄기세포는 세포 치료 목적을 위한 재료로써 매우 큰 잠재력을 가지고 있으며, 이러한 잠재력의 실현을 위해서 세포의 운명에 결정적인 역할을 하는 요소들을 확인하고 특정 세포의 대량 생산을 위한 방법을 개발하여야 한다. 조혈과정은 폭넓게 연구되어 왔으며, 배아 줄기세포로부터 조혈세포의 분화는 lineage commitment에 관한 연구에 좋은 모델이 된다. 본 연구에서는, 두 종류의 마우스 배아 줄기세포주 TC-1과 B6-1를 이용하여 그 특성과 조 Embryonic stem(ES) cells have tremendous potential as a cell source for cell-based therapies. Realization of that potential will depend on our ability to understand and manipulate the factors that influence cell fate decision and to develop methods for getting enough cell numbers for clinical applications. Hematopoiesis has been widely studied, and hematopoietic differentiation from ES cells is a good model to study lineage commitment. In this study, we investigated stemness and compared the efficiency of hematopoietic differentiation using two different mouse embryonic stem cell lines TC-1 and B6-1. Although the two cell lines showed known stem cell properties with minor differences, the embryoid body formation efficiency in methylcellulose was much higher in TC-1 than B6-1. When measured potentials of hematopoietic differentiation using functional(colony-forming cell) and phenotypic(specific marker expression) assays, we found that TC-1 can differentiate into hematopoietic cells in methylcellulose culture but B6-1 cannot. These results imply that we can improve the efficiency of hematopoietic cell differentiation by selection of proper cell lines and this may be also applied in the differentiation of human embryonic stem cells.