-G308A Tumor Necrosis Factor-α 유전자 다형성과 주요 우울장애의 연합연구

배치운,오해정,채정호,박원명,전태연,한훈,김광수 大韓神經精神醫學會 2003 신경정신의학 Vol.42 No.2

Objectives : The present study was to examine possible association between the - G308A tumor necrosis factor (TNF)-α gene polymorphism and major depressive disorder (MDD) in Korean. Methods : 108 inpatients with MDD and 125 healthy controls participated in this study. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism. Results : Genotype and allele distributions in patients with MDD (p=0.02, P=0.01, respectively), were significantly different from those of the controls. Conclusion : The present study suggests that the -G308A TNF-α gene polymorphism may have a potential role for the susceptibility to MDD in Korean population.

양극성 장애와 Monocyte Chemoattractant Protein-1 유전자 다형성

배치운,김태석,장우영,김정진,이창욱,이수정,전태연,이철,백인호 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.5

Objectives : Several studies suggested that cytokines could be involved in the pathogenesis of mood disorders, while data for bipolar disorder is relatively deficient (BD). BD, Knowned to have a inherited tendency, has been considered to be related with T-helper cell system. This study was designed to investigate the association between polymorphism of monocyte chemoattractant protein-1 (MCP1) gene at promoter-2518 and BD. Methods : Patients with BD (n=92) in accordance with DSM-FV criteria and control subjects (n=114) participated in this study. DNA was extracted from whole blood and the MCP-1 gene promoter region was amplified by polymerase chain reaction-based method. Results : Genotype and allele distributions in patients with BD were not different from those of control subjects (p=0.587 ; p=0.384). Genotype and allele distributions in accordance to presence or absence of suicide attempt (p=0.423), family history (p=0.733) and psychotic feature (p=0.436) were not different between the two groups. Conclusion : Present study suggests that the MCP1 promoter -2518 polymorphism may not affect the susceptibility and clinical diversity of the development of BD.

주요 우울장애에서 Cytotoxic T Lynphocyte Antigen(CTLA-4) 유전자의 다형성

전태연,배치운,김병균,채정호,박원명,김광수,유태열,한훈 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.5

연구목적 : 세포의 면역 기능 조절에 관여하는 CTLA-4의 유전자 다형성을 분석하여 주요 우울장애와의 면역 유전학적 관련성을 알아보고자 하였다. 방 법 : DSM-Ⅳ진단 기준에 따라 주요 우울장애로 진단된 환자 77명을 선정하였으며 가톨릭대학교 의과대학 조혈모세포은행에서 보유하고 있는 149명의 정상 한국인 자료를 대조군으로 사용하였다. 전혈에서 DNA를 추출하였으며 중합효소반응으로 CTLA-4유전자 exon 1 부위를 증폭한 후 SSCP방법으로 유전?? 형별 분석하였다. 결 과 : 대조군과 주요 우울장애 환자군 사이의 CTLA-4 유전자형과 대립유전자의 발현 빈도를 비교시 유의한 차이가 없었다. 결 론 : 본 연구 결과 exon 1 CTLA-4 유전자형 및 대립유전자의 발현빈도는 주요 우울장애 환자군과 정상 대조군 사이에 차이가 없어, exon 1 영역은 후보 유전자로서의 가능성이 없었다. 향후 연구에서는 다양한 변인들에 따라 대상군을 임상적으로 동질성을 지닌 집단으로 세분하여 조사대상 유전자와의 관련성에 대한 연구가 이루어져야 하겠다. Objectives : This study was carried out to explore the relationship between major depressive disorder and CTLA-4 which is related to the immunologic function such as T cell regulation. Methods : Among the korean patients diagnosed as major depressive disorder according to DSM-Ⅳ, 77 patients without neurological illness, hormonal disorder, or comorbid mental illness were selected. The stored data of 149 normal Koreans from the Catholic Hemopoietic Stem Cell Bank of Korea, were used as a normal control group. The data of Korean control group were compared with those of the studies of different ethnics. DNA was extracted from whole blood and the exon I region of CTLA-4 gene was amplified by polymerase chain reaction. Gene typing was performed by using SSCP and then, the results were assessed. Results : There were no significant differences in genotype frquencies of CTLA-4*G/G, CTLA-4*G/A, and CTLA-4*A/A between the patients with major depressive disorder and the control group in Korean population(48.1% vs 46.3%, 41.6% vs 39.6%, 10.3% vs 14.1%, respectively).There were no significant differences in allelic frequencies of CTLA-4*G and CTLA-4*A between the patients with major depressive disorder and the control group in Korean population(68.8% vs 66.1%, 31.2% vs 33.9%, respectively). Conclusion : Considering negative result for the association of the exon I polymorphism of CTLA-4 gene with major depressive disorder in this study, the exon I polymorphism does not appear to be possible candidate gene for major depressive disorder. Moreover, further systematic researches including diverse clinical variables would required.

저염도 음식물 쓰레기를 이용한 퇴비시여가 배추의 생육에 미치는 영향

전윤태,박길환,조경철,김희경,지연태,정순주 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-

This study was conducted to investigate the changes of physico-chemical properties of soil applied with compost made by food wastes and its effect on the growth and yield of Chinese cabbage. Chinese cabbage was grown in the soil treated with food waste compst(FWC) of 0, 0.625, 1.25, 2.5, 5 and 10㎏/3.3㎡. As the amount of FWC increased, number of leaves, leaf area and fresh weight increased where application of 5㎏ and 10㎏/3.3㎡ showed vigorous plant growth in the late growing period. The pH of soil by application of FWC was not raised, which was similar to the soil before treatment. Organic matter increased in soil by increasing the amount of FWC. However, concentration of Cu and Pb in the soil reduced. The concentration of available phosphate(P) was lower in the soil treated with FWC than in the soil before treatment, which means that a large amount of P may be uptaken by plant. This study suggested that FWC should be an alternative to chemical fertilizer for vegetable production.

양극성 장애 1형에서 종양괴사인자 베타 유전자 다형성

전태연,이경욱,배치운,김원,우영섭,채정호,박원명 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : Bipolar disorder is known to have a high genetic predisposition. Recently, the main focus of etiologic studies in bipolar disorder has been concentrated on molecular genetic approach including gene polymorphism analysis. The present study was conducted to investigate whether TNFB polymorphism is associated with bipolar I disorder in the Korean population. Methods : 89 bipolar I disorder patients diagnosed by DSM-IV criteria were assigned as the patient group and 202 normal population, matched on age and sex from Catholic hemopoietic stem cell bank (Seoul, Korea), were enrolled as the control group in this study. Genotyping was performed by a polymerase chain reaction-restriction fragment length polymorphism method. All data was analyzed by x² test. Results : There were no significant differences in frequency of TNFB*1/1,TNFB*1/2 and TNFB*2/2 between bipolar I disorder patient group and normal control group. The frequency of TNFB*1 was not statistically different between bipolar I disorder patient group and normal control group. Conclusion : The difference of frequency in TNFB*1/TNFB*2 gene between the bipolar I disorder gropup and the normal contro1 could not be verified. The present result suggested that the gene polymorphism of TNFB may not play a significant role in susceptibility to bipolar I disorder. Studies with a larger number of subjects from different ethnic backgrounds, considring clinical phenotype and controlling various factors, should be launched to further determine the role of TNFB in bipolar I disorder.

주요 우울증에서 종양괴사인자-베타 유전자의 다형성

전태연,배치운,김영호,장계호,이정태,박원명,김광수 대한신경정신의학회 2000 신경정신의학 Vol.39 No.6

연구목적 : 주요 우울증은 역학적 유전연구 등을 통하여 유전적 영향이 높은 것으로 알려져 있으며 최근에는 분자 유전학적 연구로 유전자 다형성과 질병의 연관성을 밝히는 것이 정신질환의 유전학적 연구에서 중요한 부분을 차지하고 있다. 이에 본 연구는 중추신경계와 면역계간의 상호작용에 관여하는 싸이토카인 중 TNF-β 유전자의 다형성을 분석하여 주요 우울증과의 유전학적 관련성을 알아보고자 하였다. 방 법 : DSM-IV에 의하여 주요 우울증으로 진단된95명을 환자군으로 선정하였고 가톨릭조혈모세포정보은행에서 보유하고 있는 정상 한국인 202명의 자료를 정상 대조군으로 사용하였다. 전혈에서 DNA를 추출하고 TNF-β 유전자 부위를 증폭한 후 제한효소 Nco Ⅰ으로 절단하여 555bp와 185bp의 절편을 갖는 TNFB*1과 Nco Ⅰ절단부가 없는 740bp의 절편 TNFB*2등 2가지 대립유전자의 제한효소절편길이 다형성을 조사하였다. 모든 자료의 분석은 x²검증을 이용하였다. 결 과 : 1) 주요 우울증과 정상 대조군 간에 TNFB유전자인 TNFB*1/1, TNFB*1/2 및 TNFB*2/2의 발현 빈도에는 유의한 차이가 없었다. 2) 두 군 간에 TNFB*1 과 TNFB*2 두 대립유전자의 빈도에는 유의한 차이가 없었다. 결 론 : 본 연구에서는 주요 울울증군과 정상 대조군 간에 TNFB*1과 TNFB*2의 두 대립유전자 발현 빈도에 유의한 차이를 발견하지 못하였다. 따라서 TNFB 유전자다형성과 주요 우울증과의 유전학적인 연관성이 없었다. 향후 연구에서는 다양한 임상변인을 포함하여 보다 통합적이고 체계적인 연구가 이루어져야 할 것으로 생각되었다. Objective : Major depressive disorder is known to have high genetic predisposition and the main focus of recent genetic studies in major depressive disorder has been concentrated on association studies between genetic polymorphism and disease, since molecular genetic methods have been developed. This study was designed to investigate the relationship between major depressive disorder and immunogenetic influences by analyzing polymorphism of TNFB gene, which is involved in interaction of immune system and CNS. Method : 95 persons who had been diagnosed of major depressive disorder were assigned as patient group and, 202 data obtained from Catholic hemopoietic stem cell bank, College of medicine, the Catholic University of Korea, were used as normal controls in this study. DNA was extracted from whole blood, thereafter amplified by polymerase chain reaction, and digested by Nco Ⅰ.After that procedure, we obtained and assessd restriction fragment length polymorphism of two alleles, TNFV*1 which has 555bp and 185bp fragments and carries the Nco Ⅰ restriction site, and TNFB*2 of 740 bp fragment lacks the Nco Ⅰ restriction site. All data were analyzed by x²test with two-tailed Fisher's exact test. Results : 1) The frequencies of TNFB*1/1, TNFB*1/2, and TNFB*2/2 were not statistically different between major depressive disorder patients and control group. 2) The frequencies of TNFB*2 and TNFB*1 were not statistically different between major depressive disorder patient group and normal control group. Conclusion : We did not verified the differences of frequency in TNFB*1/TNFB*2 gene between the major depressive disorder and normal controls, respectively. Consequently, there is no genetic relationship between major depressive disorder and gene polymorphism of TNFB. We do suggest that further systematic studies including various clinical variables should be conducted.

정신분열병과 Glutathione S-Transferase M1유전자 다형성

배치운,조자현,김연주,김태석,김정진,이창욱,이수정,전태연,이철,백인호 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.5

Objectives : This study aimed to test the possible association between Glutathione S-Transferase Ml gene (GSTMl) variants and schizophrenia. Methods : One hundred and eleven inpatients with schizophrenia and 130 healthy controls were recruited. Genotyping was performed by polymerase chain reaction-based method. Results : The GSTMl null genotype was significantly more frequent in patients with schizophrenia than in controls (p=0.014, odd ratio=1.93, 95% confidence interva1=1.115-3.351), while GSTMl genotype vahants were not associated with either tardive dyskinesia (TD) or total Abnormal Involuntary Movement Scale (AIMS) scores. Conclusion : The present study suggests that the GSTMl polymorphism may confer susceptibility to the development of schizophrenia but not to TD, at least in Korean population.

Monocyte Chemoattractant Protein-1[MCP1] -2518 유전자 다형성과 주요 우울장애

배치운,이지현,신윤경,김태석,김정진,이창욱,이수정,전태연,이철,백인호 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Object : This study was designed to examine the association between monocyte chemoattractant protein-l (MCPl) -2518poly morphism and major depressive disorder (MDD). Methods : Ninety patients with MDD and 114 healthy controls participated in this study. Genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism method. Results : Genotype and allele distributions in patients with MDD were significantly different from those of the controls, In particular, subjects with the allele A were found to have an increased risk of MDD. Conclusion : The present study suggests that the MCPl -2518 polymorphism may have a potential role for susceptibility to MDD in the Korean population and thus calls for consecutive studies in order to pile up the data with larger different ethnic background.

음식물 쓰레기 퇴비가 오이 발아와 유묘생장에 미치는 영향

박진영,조경철,김희경,지연태,정순주 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-

This study was conducted to find out toxicity of compost made of food waste to cucumber seed germination and seedlings growth when it was mixed with Mixlite for bed substrate. Mixlite was solely used as bed soil, or mixed with food waste compost ranging 1% to 100% (w/w). Germination percentage and seedling growth of cucumber were examined in 4 weeks after seedling. When cucumber seeds were sowed in Mixlite mixed with food waste compost at 100, 50, 30, 20, 10 or 1%, germination percentages were 30, 36, 38, 46, 74 and 84% compared to with control(100%), respectively. It showed that germination percentage percentage was adversely affected by the amount of food waste composed mixed in the bed substract. Plant height and hypocotyls diameter, root length and root fresh weight, hypocotyls fresh weight and cotyledon fresh weight of cucumber seedling were similarly adversely affected by the amount of food waste compost. Optimum content of the food waste compost for cucumber nursery substrate was found to be 1% of Mixlite.

미주신경자극술(Vagus Nerve Stimulation)의 신경정신과적 응용

채정호,배치운,박원명,전태연,김광수,Mark S. George 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.3

연구목적: 최근 신경생물학과 두뇌 조영술 발달에 따라 두뇌에 비침습적 자극을 줌으로써 신경회로를 변화시킬수 있는 새로운 신경정신과적 치료방법의 개발이 태동하고 있다. 그 중 두뇌와 다양한 연결을 구축하고 있는 제 10번 두개신경인 미주신경을 직접 자극할 수 있는 미주신경자극술(Vagus Nerve Stimulation:이하푼)이 개발되어 간질 및 일부 신경정신과적 질환에서 시술되고 있다. 본 논문의 목적은 VNS의 발달과정, 시술방법, 신경정신과적 응용, 및 부작용 등에 대하여 개괄하고 그 현황과 미래를 고찰하는데에 있다. 방 법 : 광범위한 문헌 검색과 현재 VNS를 시술하고 있는 기관의 세계적 전문가와의 논의를 통하여 조사 및 정리하였다. 결 과 : 현재 VNS는 치료 저항성 간질의 치료 방법의 하나로 공인되어 있으며 일부 신경정신과적 질환에서 치료적으로 사용될수 있는 가능성이 제시되고 있다. 결 론 : 두뇌를 자극할 수 있는 새로운 방법인 VNS는 우울증을 비롯한 일부 신경정신과적 질환에서 유의한 치료효과를 거둘 가능성이 있으며, 두뇌-행동 관계를 이해하는데에 매우 흥미있는 방법으로 향후 많은 관심을 끌게 된 것이다. Objectives : Several novel and minimally invasive techniques to stimulate the brain have recently developed. Among these newer somatic interventions, vagus nerve stimulation(VNS) is regarded as a promising tool in the treatment of various neuropsychiatric disorders. This article reviews the history, methodology, and the future of VNS technique and its emerging research and therapeutic applications in the field on neuropsychiatry. Methods : Wide ranged literature reviews and discussion with pioneering researchers were performed. Results : VNS has been used in the treatment of refractory seizure disorder and depression. There are some reasons to hope that VNS might have other therapeutic applications in neuro-psychiatric disorders, as well as advanced understanding about the pathophysiology of these disorders. Conclusion : Regardless of its clinical role as a new therapeutic technique, the capacity of VNS as a research tool to alter brain activity should lead to important advances in the understanding of brain-behavior relationships.