A Study on the Screening of Children at Risk for Developmental Disabilities Using Facial Landmarks Derived From a Mobile-Based Application

Sang Ho Hwang,Yeonsoo Yu,Jichul Kim,Taeyeop Lee,Yu Rang Park,Hyo-Won Kim 대한신경정신의학회 2024 PSYCHIATRY INVESTIGATION Vol.21 No.5

Objective : Early detection and intervention of developmental disabilities (DDs) are critical to improving the long-term outcomes of af-flicted children. In this study, our objective was to utilize facial landmark features from mobile application to distinguish between children with DDs and typically developing (TD) children. Methods : The present study recruited 89 children, including 33 diagnosed with DD, and 56 TD children. The aim was to examine the effectiveness of a deep learning classification model using facial video collected from children through mobile-based application. The study participants underwent comprehensive developmental assessments, which included the child completion of the Korean Psychoedu-cational Profile-Revised and caregiver completing the Korean versions of Vineland Adaptive Behavior Scale, Korean version of the Child-hood Autism Rating Scale, Social Responsiveness Scale, and Child Behavior Checklist. We extracted facial landmarks from recorded vid-eos using mobile application and performed DDs classification using long short-term memory with stratified 5-fold cross-validation. Results : The classification model shows an average accuracy of 0.88 (range: 0.78–1.00), an average precision of 0.91 (range: 0.75–1.00), and an average F1-score of 0.80 (range: 0.60–1.00). Upon interpreting prediction results using SHapley Additive exPlanations (SHAP), we verified that the most crucial variable was the nodding head angle variable, with a median SHAP score of 2.6. All the top 10 contributing variables exhibited significant differences in distribution between children with DD and TD (p<0.05). Conclusion : The results of this study provide evidence that facial landmarks, utilizing readily available mobile-based video data, can be used to detect DD at an early stage.

Polymorphisms in Apoptosis-Related Genes and Survival of Patients with Early-Stage Non-Small-Cell Lung Cancer

Lee, Eung Bae,Jeon, Hyo-Sung,Yoo, Seung Soo,Choi, Yi Young,Kang, Hyo-Gyoung,Cho, Sukki,Cha, Sung-Ick,Choi, Jin Eun,Park, Tae-In,Lee, Byung-Heon,Park, Rang-Woon,Kim, In-San,Kang, Young Mo,Kim, Chang Ho Springer - Society of Surgical Oncology 2010 Annals of Surgical Oncology Vol.17 No.10

Prevalence of Helicobacter pylori Infection in Patients with Peptic Ulcer Diseases and Non-Ulcer Dyspepsia

Hyo Rang Lee,Ki Soo Han,Byung Chul Yoo,Sill Moo Park,Young Joo Cha 대한내과학회 1993 The Korean Journal of Internal Medicine Vol.8 No.2

풍선덩굴 잎 추출물의 항산화 효과 및 성분 분석

정효진 ( Hyo Jin Jeong ),김아랑 ( A Rang Kim ),이건수 ( Keon Soo Lee ),박소현 ( So Hyun Park ),신혁수 ( Hyuk Soo Shin ),이상래 ( Sang Rae Lee ),송바름 ( Ba Reum Song ),이윤주 ( Yun Ju Lee ),안현진 ( Hyun Jin An ),이재덕 ( Jae Duk 대한화장품학회 2017 대한화장품학회지 Vol.43 No.2

본 연구에서는 풍선덩굴 잎 추출물 및 그 분획물에 대하여 항산화 활성 평가와 성분 분석을 수행하였다. 본 실험에는 풍선덩굴 건조 잎의 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획을 사용하였으며, 각각의 수율은 16.4, 0.9 및 0.3%로 나타났다. 자유라디칼 소거활성(1,1-diphenyl-2-picrylhydrazyl, DPPH, FSC₅₀)은 에틸아세테이트 분획(92.5 μ g/mL)이 가장 큰 것으로 나타났으며, 이때 대조군인 (+)-α-tocopherol의 FSC₅은 8.9 μ g/mL이었다. Fe³⁺-EDTA/H₂O₂계를 이용한 활성산소 소거활성(총항산화능, OSC₅₀)은 아글리콘 분획(4.2 μ g/mL)에서 가장 크게 나타났으며, 대조군인 L-ascorbic acid (1.5 μ g/mL)와 유사한 효과를 나타내었다. ¹O₂로 유도된 사람 세포 손상에 대한 보호효과 측정에서 풍선덩굴 잎 추출물 및 분획은 모두 농도 의존적(5.0-25.0 μ g/mL)으로 세포보호효과를 나타냈다. 추출물/분획물 중에서 아글리콘 분획(τ₅₀, 76.4 min)이 가장 큰 세포보호효과를 나타내었다. TLC, HPLC, LC/ESI-MS를 이용하여 풍선덩굴 잎추출물 중 에틸아세테이트 분획물에 대하여 성분 분석을 실시하였다. 그 결과, apigenin-7-O-glucuronide, apigenin-7-glucosdie 및 quercitrin hydrate 등의 플라보노이드가 함유되어 있음을 확인하였다. 이상의 결과 들은 풍선덩굴 잎 추출물 또는 분획이 항산화 기능성 화장품 원료로서 응용 가능성이 있음을 시사한다. In this study, the antioxidant effect and component analysis for extract and fractions of Cardiospermum halicacabum leaf were investigated. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from dried C. halicacabum leaf. The yields of extract and fractions were 16.4, 0.9 and 0.3% per dried powder, respectively. DPPH (1,1-phenyl-2-picrylhydrazyl) radical scavenging activity (FSC₅₀) of ethyl acetate fraction (92.5 μg/mL) was the greatest radical scavenging activity, but lower than (+)-α-tocopherol (8.9 μg/mL). In reactive oxygen species (ROS) scavenging activity (total antioxidant capacity, OSC₅₀) on ROS generated in Fe³⁺-EDTA/H₂O₂ system, aglycone fraction (4.2 μg/mL) was the highest total antioxidant capacity and similar to L-ascorbic acid (1.5 μg/mL). The cellular protective effects of C. halicacabum leaf extract and fractions on the ¹O₂-induced cellular damage of human erythrocytes were exhibited at all concentration-dependent (5.0-25.0 μg/mL). Especially, aglycone fraction (τ₅₀, 76.4 min) in 25.0 μg/mL showed the most protective effect among extracts. Components of the ethyl acetate fraction obtained from C. halicacabum extracts were analyzed by TLC, HPLC chromatogram and LC/ESI-MS. Results showed that the ethyl acetate fraction contained some flavonoids, such as apigenin-7-O-glucuronide, apigenin-7-glucosdie and quercitrin hydrate. These results suggest that the extracts and fractions of C. halicacabum leaf may be applied as antioxidant functional cosmetic raw materials.

Stabilization of HDAC1 via TCL1-pAKT-CHFR axis is a key element for NANOG-mediated multi-resistance and stem-like phenotype in immune-edited tumor cells

Woo, Seon Rang,Lee, Hyo-Jung,Oh, Se Jin,Kim, Suyeon,Park, Sang-Hyo,Lee, Jaeyoon,Song, Kwon-Ho,Kim, Tae Woo Elsevier 2018 Biochemical and biophysical research communication Vol.503 No.3

<P><B>Abstract</B></P> <P>Cancer immunoediting enriches NANOG expression in tumor cells, resulting in multi-drug resistance and stem-like phenotypes. We previously demonstrated that these NANOG-associated phenotypes are promoted through HDAC1 transcriptional upregulation. In this study, we identified that NANOG also contributes to the stabilization of HDAC1 protein through the AKT signaling pathway. NANOG-AKT axis leads to phosphor-dependent inactivation of CHFR, an E3 ligase for HDAC1 protein, and thereby inhibiting the ubiquitin-mediated degradation of HDAC1. Furthermore, AKT inhibition disrupts HDAC1 WT-mediated phenotypes but had no effect on the phenotypes mediated by HDAC1 FM, a mutant that is unable to interact with CHFR. Critically, we applied a catalytic dead mutant, HDAC1-H141A, to uncover that HDAC1 confers immune-resistance, drug-resistance and stem-like phenotype in tumor cells through its catalytic activity. Collectively, our results establish a firm molecular link in immune-edited tumor cells among NANOG, AKT, CHFR, and HDAC1, identifying HDAC1 as a molecular target in controlling NANOGHIGH immune-refractory cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> NANOG contributes to stabilization of HDAC1 protein through AKT signaling pathway. </LI> <LI> NANOG-AKT axis leads to phosphor-dependent inactivation of CHFR, which is an E3 ligase for HDAC1 protein. </LI> <LI> HDAC1 confers immune-resistance, drug-resistance and stem-like phenotype through its catalytic activity. </LI> <LI> AKT inhibition disrupts HDAC1-mediated multi-resistance and stem-like phenotype. </LI> </UL> </P>

Analysis of 89Sr, 90Sr in Soil Sample Using Crown Ether/Chloroform Solvent Extraction Method

Lee,Chang-Woo,Kim,Sang-Bog,Choi,Kun-Sik,Kim,Sam-Rang,Choi,Yong-Ho,Park,Hyo-Guk,Lee,Myong-Ho,Hong,Kwang-Hee 대한방사선 방어학회 1996 방사선방어학회지 Vol.21 No.1

환경시료중 토양에 대한 방사성 스트론튬인 89Sr과 90Sr의 분석을 위해 Ca과 Sr을 분리하기 위한 방법으로 Crown ether를 이용한 추출방법을 사용하여 기존의 발연질산법과 비교하여 보았다. Crown ether를 이용한 추출방법을 기존으 발연질산법과 비교하여 보았을때 높은 Sr수율을 보였을뿐만 아니라 분석에 소요되는 시간과 절차가 훨씬 빠르고 간단했다. Crown ether를 이용한 추출방법으로 완전주변 토양에 대한 방사성 스트론튬의 분석을 수행하였으며, 방사성핵종 분석에 의한 환경감시의 효율적인 수행을 위한 스트론튬의 분석법을 제시하였다. For the determination of radiostrontium, 89Sr and 90Sr in environmental soil sample, a solvent extraction method for the separation of Ca and Sr from matrix using crown ether was investigated. In comparison with the existing frming nitric acid method, the extraction method showed high chemical yeeld of strontium and provided simple and rapid analytical steps. The new analytical method applied to the determination of radiostrontium in some soil sample around a nuclear power station to show that the analytical procedure is readily applicable to the practical radioactivity monitoring.

Recycling of Waste Rock as a Filler to Prevent Ground Subsidence

Lee, Woo Keun,Ban, Hyo Jin,Im, I Rang Trans Tech Publications, Ltd. 2007 Materials science forum Vol.544 No.-

<P>In this study, we investigate to use waste rock as a filler for filling the cavity of abandoned mines. We discuss that waste rock can be used as a filler in the side of environmental and structural stability. Waste rocks from four mines, such as Samgi, second Yeonwha, Dongwon and Dogye, are used. Leaching tests are conducted to check the environmental stability by four methods of Soil Leaching Test, MALT, TCLP and KSLT. According to the results, Cu, Cd, Cr and As were not detected and other metals leached below 0.5 ppm. Compressive strength of waste rock and crushed waste rock are measured to evaluate the structural stability. Mixtures of crushed waste rock are used to evaluate the possibility of enhancing compressive strength. Mixture 1 is composed of 11.1% of above 13.2mm, 22.2% of 9.5-13.2mm, 33.3% of 6.7-9.5mm, 22.2% of 3.5-6.7mm and 11.1% of 2.0-3.5mm. And mixture 2 is composed of 50% of above 13.2mm and 50% of 9.5-13.2mm. Compressive of the latter was more than that of the former. Waste rock can be probably used as a filler, considering the compressive strength of commercialized filler, or 40 Kg/cm2.</P>

Proton Conduction in a Tyrosine-Rich Peptide/Manganese Oxide Hybrid Nanofilm

Lee, Jaehun,Choe, Ik Rang,Kim, Young-O,Namgung, Seok Daniel,Jin, Kyoungsuk,Ahn, Hyo-Yong,Sung, Taehoon,Kwon, Jang-Yeon,Lee, Yoon-Sik,Nam, Ki Tae Wiley - VCH Verlag GmbH & Co. KGaA 2017 Advanced Functional Materials Vol.27 No.35

제대혈의 적혈구 제거 방법에 관한 연구 : 제대혈 은행 설립을 위한 기초 연구 Basic Study for the Establishment of Cord Blood Bank

김효정,서철원,김상희,김상위,김성배,김강욱,조화정,민영주,박진희,김암,이인식,이필량,지현숙,서종진,강위창,이정신,김우건 대한조혈모세포이식학회 2000 대한조혈모세포이식학회지 Vol.5 No.1

배경:제대혈에서 적혈구를 제거하는 것은 제대혈 은행의 효율적인 운영 및 제대혈이식시 ABO 부적합 수혈, DMSO 독성 등의 부작용을 해결하기 위한 이상적인 방법이다. 저자들은 동일한 제대혈로 여러가지 적혈구 제거법을 동시에 시행하여, 이들에서 CD34양성 세포의 수득률 및 조혈세포 집락형성능을 비교하여 제대혈 은행 설립의 기반 기술을 확립하고자 하였다. 방법:제왕절개 직후 헤파린 처리된 용기에 제대혈을 채취하였다. 제대혈은 5개의 용기에 분산하여 실온에서 보관하였고 12시간 이내에 적혈구를 분리하였다. 적혈구 제거 방법으로 Ficoll 비중 분리법, gelatin 침전법, 적혈구 용혈법, starch 침전법을 각각의 제대혈에서 시행하였다. 전혈과 적혈구가 제거된 제대혈에서의 세포 생존율, 적혈구 제거율과 단핵구 및 CD34양성 세포의 수득률, 조혈세포 집락형성능을 비교 분석하였다. 결과:18례에서의 평균 제대혈 채취양은 81.3 mL(범위 42~128 mL)이었다. 서로 다른 적혈구 제거법들간에 세포 생존률과 적혈구 제거율은 차이가 없었다. 단핵구 수득률은 3% gelatin침전법(47.7±14.5%)이 다른 적혈구 제거법보다 유의하게 높았다. 3% gelatin침전법은 Ficoll비중 분리법과 Starch침전법에 비해 CD34 양성 세포수가 통계적으로 유의하게 높았다(36.1±5.4×10³/mL, 15.0±3.5×10³/mL, 20.7±4.2×10³/mL). 또한 CFC의 집락수도 3% gelatin침전법에서 Ficoll 비중 분리법과 적혈구 용혈법에 비해 우수한 결과를 보였다(52.0±7.1×10²/mL, 32.8±4.7×10²/mL, 36.8±5.7×10²/mL). 그러나 제대혈 전혈의 CD34 양성 세포수(65.7±11.2×10³/mL) 및 CFC의 집락수(117.8±15.8×10²/mL)를 적혈구 제거법을 이용한 실험군과 비교하였을 때 전혈군에서 높은 것을 알 수 있었다. 결론:3% gelatin 침전법은 다른 적혈구 제거법과 비교해 볼 때 더 우수한 단핵구, CD34 양성 세포 수득률 및 조혈세포 집락형성능을 보였다. 그러나 다른 보고들과는 달리 3% gelatin 침전법은 CD34양성 세포 및 CFC 집락수의 절대값이 제대혈 전혈과 비교하여 의미있게 낮았다. 이러한 결과는 시약의 종류와 실험방법의 차이에 기인하는 것으로 사료되며, 최상의 세포 수득률을 얻기 위하여 적혈구 분리 방법의 표준화를 위한 연구가 진행되어야 할 것으로 생각된다. Background:Efficient volume reduction is important to make a cost-effective umbilical cord blood (UCB) banking system. The aim of this study was to find a method of red cell depletion of UCB without major losses of the hematopoietic progenitor-CD34+ cells. Methods:Eighteen cord blood samples were collected in heparinized bottle immediately after Cesarean section. Five aliquots of each cord blood were stored at room temperature and processed within 12 hours. We used 4 different RBC depletion methods (Ficoll density gradient separation, gelatin sedimentation, red cell lysis, starch sedimentation) and compared the results of viability, RBC reduction rate, mononuclear cell (MNC) recovery rate, CD34+ cells and colony forming unit (CFC).Results: The mean collected cord blood volume was 81.3 mL (range 42~128 mL). There were no differences of viability and RBC reduction rate among groups. MNC recovery rate were significantly higher in gelatin group (47.7±14.5%) than other RBC depletion methods. Gelatin group had significantly higher CD34+ cell count than Ficoll and starch group (36.1±5.4×10³/mL, 15.0±3.5×10³/mL, 20.7±4.2×10³/mL) and higher CFC count than Ficoll and RBC lysis group (52.0±7.1×10²/mL, 32.8±4.7×10²/mL, 36.8±5.7×10²/mL). But the results of CD34+ cell count (65.7±11.2×10³/mL) and CFC count (117.8±15.8×10²/mL) of untreated control group are significantly higher than those of all other treated groups. Conclusion:Umbilical cord blood processed with 3% gelatin sedimentation has higher recovery rate of MNC, CD34+, cell and CFC than other groups. Unlike other reports, the absolute numbers of CD34+ cell and CFC count of 3% gelatin sedimentation method are significantly lower than those of untreated control group. Different laboratory processes with gelatin could be one cause of these differences, a study for standardized laboratory methods should be established to obtain higher recovery rate of hematopoietic cells after RBC depletion.

PCM cool roof system의 도시열섬현상 저감 효과 분석

이효은(Lee, Hyo Eun),양영권(Yang, Young Kwon),박보랑(Park, Bo-Rang),박진철(Park, Jin Chul) 대한건축학회 2017 대한건축학회 학술발표대회 논문집 Vol.37 No.1