VP-16으로 성공적으로 치료된 위 Kaposi 육종 1예

양웅석,송근암,조몽,이재승,송철수,김형욱,김영민,이수봉,김태오,이성훈 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.4

The skin is an uncommon site of distant metastasis from any internal malignancy, and the incidence of metastatic skin lesions as the first symptom of disease is only 0.8% in patients with all systemic malignancies. Furthermore, cutaneous metastasis from adenocarcinoma of the stomach has been found to be extremely rare. A 35-year-old female patient was admitted due to multiple cutaneous nodules in her chest, abdomen, and back. A gastroendoscopic examination and biopsy was made according to the results of skin pathologic findings. Stomach and skin biopsy results revealed a signet ring cell type of adenocarcinoma. A case of gastric adenocarcinoma in which metastatic skin nodules appeared as the first sign of disease, is here in reported with a review of related literature.

토양에서 분리한 세균의 T-2 toxin 분해능

이웅수 충주대학교 2006 한국교통대학교 논문집 Vol.41 No.-

Trichothecenes are a chemically related group of toxic fungal metabolites produced by a number of species of the fungi such as Fusarium, Cephalosporium, Trichothecium, Myrothecium, Stachybotrys and Trichoderma, and are responsible for mycotoxicoses as causative agents in a wide variety of animal and human health problems. T-2 toxin is a potent cytotoxic and immunodepressive trichothecene mycotoxin, and a causative agent of moldy corn toxicosis and alimentary toxic aleukia(ATA). T-2 toxin induces acute toxicity such as leukocytosis, hemorrhage and dermal edema, followed by death in animals. In order to clarify the systems of metabolism, degradation and detoxification of T-2 toxin, one of the naturally occuring trichothecene mycotoxins, this research was carried out. 20 soil samples were examined for the degradation activity of T-2 toxin by incubation of 30℃ for 7 days with medium supplemented 0.2% T-2 toxin as a sole source of carbon and energy by enrichment culture technique. 10 strains that have T-2 toxin degradation activity were isolated from soil samples. Among 10 strains, NO. CJNU-10-1 strain has the most potent activity for the degradation of T-2 toxin. The metabolic pathway of T-2 toxin was investigated with this strain. This strain metabolized T-2 toxin to the following weak or non-toxic compounds ; T-2 toxin→HT-2 toxin→T-2 triol→unknown metabolite(s). The present study has revealed that bacteria, No. CJNU-10-1 strain, isolated from soil has ability of deacetylating, degradation and detoxification T-2 toxin, and a subsequent study for the taxonomical characterization and identification of No. CJNU-10-1 strain is currently under way.

集團給食所 및 家庭의 廚房에서 使用하는 행주의 衛生學的 處理에 관한 硏究

李雄洙,崔錫南 충주대 2003 産業科學論文集 Vol.11 No.-

This study was carried out to find out the method on hygienic treatment of dishcloth used at the cookroom of mass meal service center and household in order to prevent food poisoning and oral infectious disease. This research was examined with three methods(washing, boiling and chlorine solution treatment) based on materials for the questionnaire by 28 persons who engaged in mass meal service center and telephone interview by 100 housewives. The indicator bacteria on the fecal contamination in food hygiene such as Escherichia coli, Staphylococcus aureus, Brochothrix thermosphacta, Salmonella typhimurium, vibrio parahae-molyticus and pseudomonas aeruginosa, and Shigella sonnei, one of oral epidemic bacteria, were used in this study. The results of this study related to food hygienic and micro-biological tests were the following : 1) By washing treatment, the indicator bacteria and Shigella sonnei were detected less 1/2 to 1/4 than initial viable cell counts. 2) After boiling treatment for 10min, E. coli, Pseudo. aeruginosa and Brocho. thermo-sphacta were found 63-340 CFU/㎖, and Staphy. aureus and Sal. typhimurium were detected 1-2CFU/℃, but Vibrio parahaemolyticus and Shigella sonnei not detected. 3) After boiling treatment for 20min, E. coli, Brocho. thermosphacta and Pseudo. aeru-ginosa were detected 4-130CFU/℃, but Staphy. aureus, Sal. typhimurium, Vibrio par-ahaemolyticus and Shigella sonnei died out completely. 4) After boiling treatment for 30min, only Pseudo. aeruginosa was found, and the other bacteria were not detected. 5) In case of soaking treatment in chlorine solution, all tested bacteria were detected to 1-2500 CFU/℃ in 80ppm solution. In 160ppm chlorine solution, Brocho. thermosphacta, Sal. typhimurium, Vibrio parahaemolyticus, Pseudo. aeruginosa and Shigella sonnei were existed to 2-32CFU/℃, but E. coli and Staphy. aureus were died out. In 320ppm chlorine solution, only Pseudo. aeruginosa was found to 13CFU/℃, and the other bacteria were not detected. In 640ppm chlorine solution, all tested bacteria were not detected. In conclusion, this study suggested that boiling over 30min and soaking in 640ppm chlorine solution are very good methods as the hygienic treatment of dishcloth contaminated with food poisoning and oral infectious disease bacteria.

수입 중국산 개나리 열매(연교)의 소염효과에 관한 연구

이응식,조재민,민윤식 충주대 산업과학기술연구소 1996 産業科學論文集 Vol.4 No.-

In order to determine whether methanol extract of Chinese Forsythiae fructus inhibite acute and chronic inflammatory responses induced with histamine and carrageenin in rat, this research was carried out. Inhibitory effect on the pigment leakage induced with histamine 50㎍ by methanol extract 0.15g per 150g body weight was shown positive result significantly. In the experiment by carrageenin paw edema method, maximum inhibitory effects were shown 1hr and 30min after orally administrated with 0.03g and 0.3g methanol extract per 150g body weight, respectively. In the inhibitory effect by methanol extract on the acute inflammation induced by granuloma pouch method, it was shown that inflammatory exudate of granuloma pouch and wet weight of granulation tissue reduced. In the chronic inflammation by granuloma pouch method, inhibitory effect was observed but significant inhibition was not observed statistically. The results of this research were shown that methanol extract of Chinese Forsythiae fructus has inhibitory effects on the acute and chronic inflammation, and were similar to those reported on Korean Forsythiae fructus.

Fusarium 속 곰팡이가 생산하는 Mycotoxin인 Nivalenol의 발암성에 관한 연구

李雄洙 충주대 산업과학기술연구소 2000 産業科學論文集 Vol.8 No.-

Nivalenol(NIV), a trichothecene mycotoxin produced by Fusarium spp., was significantly contaminated to cereals such as barley, polished barley, wheat, malt and corn, and animal feeds at high level and high frequency throughout the world. Therefore, I was carried out this research to evaluate the toxicological effects especially carcinogenicity of NIV in mice during 2 years feeding trial. In 2 years feeding period, the survival rates were 52, 52, 48 and 69% in the groups given 0, 6, 12 and 30ppm NIV, respectively. Lethal case was not observed in the 30ppm group until 18 months feeding period. Mean survival days in each group for 2 years feeding period were 565, 629, 654 and 719 days. These results indicated the increase of life span in the NIV-treated groups. The incidence rates of total tumor after 2 years feeding trial were 50, 66, 59 and 56% in each groups, respectively. Most of tumors observed in control and NIV-treated groups were lymphomas which spontaneously occurred at high frequency. There was no significant difference in the tumor incidence rate between control and NIV-treated groups. The results of this research showed that NIV was not carcinogenic substance, and reversely increased life scan according to the concentration of NIV in mice.

Anthraquinone계 화합물의 변이원성 및 유전독성 발현에 관한 연구(I) : Mutagenicity of emodin activated with cytochrome P-450 Cytochrome P-450에 의해 활성화된 emodin의 변이원성

李雄洙 忠州大學校 1998 한국교통대학교 논문집 Vol.33 No.2

It has been reported that several anthraquinone compounds possess mutagenicity and carcinogenicity. Emodin is one of the anthraquinone mycotoxins, and a constituent of several herb medicine used as laxative such as Rhei Rhizoma(rhubarb), Cassiae Semen(cassia seed) and Aloe. Emodin was selected as a model compound to solve a carcinogenic mechanism of the anthraquinone mycotoxins and to evaluate the safety of herb medicine. In this research, the mutagenicity and metabolic reaction of emodin were investigated with the purified cytochrome P-450s. In Ames test with the reconstituted cytochrome P-450 system derived from the hepatic microsomes of PB-induced rats, the mutagenicity of emodin activated with the fractionⅡ(P-448 type) was significantly higher than with the fraction Ⅰ(P-450 type). TLC analysis of the metabolites revealed that among 8 isozymes of P-450 type(Ⅰ-a, b, c, d) and P-448 type(Ⅱ-a, b, c, d), only ω-OH-emodin was detected on the TLC plate when emodin was incubated with 4 isozymes of P-450 type(Ⅰ-a, b, c, d). Whereas, 2-, 4-, 7-, ω-OH-emodin were detected in the metabolites produced by P-448 type(Ⅱ-a, b, c, d). Of 8 isozymes, Ⅱ-a possessed the highest activity in the production of 2-OH-emodin, a direct-acting mutagen. This Ⅱ-a was also represented to catalyze the hydroxylation of aflatoxin B1 and ochratoxin A into aflatoxin M1 and 4(R)-4-hydroxy-ochratoxin A, respectively. It was suggested that P-448 types play an important role in the metabolic activation of emodin.

중ㆍ장거리 선수의 최대산소섭취량, 무산소성 역치 및 혈액성분에 관한 연구

이영웅,박수연,박철빈 경희대학교 체육과학연구소 1995 體育學論文集 Vol.23 No.-

The purpose of this paper was evaluate the specificity of maximal oxygen uptake and anaerobic threshold which is indicator of cardio- respiratory function during incremental load exercise using treadmill, also. Twenty of elite middle and long distance runners participated as subjects of this research. By measurement the specificity of cardio-fulmonary function and blood component in middle and long distance runners, the conclusion and results which is obtained is as follows: 1.Maximal oxygen uptake of absolute and relative value during maximal exercise load in middle and long distance runners was display 4.23±0.421/min & 71.2/㎖/kg/min, respectively, also, maximal ventilation and maximal heart rate was expressed 146± 18.861/min & 192.25 ± 7.61beats/min, respectively. 2.Oxygen uptake and relative percentage of maximal oxygen uptake in the anaerobic threshold levels of middle and long distance runners was shown 3.55± 0.491/min(46.54㎖/kg/min) 72.81±8.86%, respectively and ventilation, heart rate and relative percentage of maximal heart rate was expressed 95.09 ± 16.951/min, 176.54 ± 9.79beats/min and 91.62 ±3.90%, respectively. 3.Concentration of plasma lactate(t-value:-3.65, p<0.003), glucose (t-value:-4.52, p<0.02) In blood component of middle and long distance runners was display the significantly difference between pre and post value after maximal incremental exercise load.

Effect of Dietary Vitamin E on the Release of Iron and Generation of Mitochondrial Hydrogen Peroxide in Rat

이웅수 忠州大學校 2003 한국교통대학교 논문집 Vol.38 No.2

In this research, whether dietary vitamin E has a direct functional effect on the production of reactive oxygen species(ROS) in mitchondria, and whether dietary vitamin E influence the formation of free iron in rat tissues were determined. Additionally, if altered iron release is linked to the degree of peroxidative damage was determined. The results clearly showed that dietary vitamin E markedly reduced hydrogen peroxide production in the mitochondria of liver and skeletal muscle of both male and female rats in a dose-dependent manner. Also, the data showed that deprivation of vitamin E resulted in an increase of free iron in the skeletal muscle and spleen of male rats and liver, skeletal muscle, heart, kidney, and spleen of female rats. Supplementation with vitamin E significantly decreased the levels of free iron in the skeletal muscle of both male and female rats. Also, the levels of free iron were significantly inversely related to the concentration of supplemented vitamin E in the skeletal muscle, kidney, and spleen of male rats and liver, skeletal muscle, heart, kidney, and spleen of female rats. Deprivation of vitamin E caused a significant increase in the levels of thiobarbituric acid reactants(TBARS) in the liver, skeletal muscle, heart, kidney, and spleen of both male and female rats. Vitamin E does-dependently decreased TBARS in the liver and kidney of male rats and liver, skeletal muscle, and kidney of female rats. The results obtained from this research indicate that vitamin E regulates mitochondrial generation of ROS and attenuates the release of iron from its protein complexes. The results also suggest that the overprodution of mitochondrial ROS is the first event leading to tissue damage observed in vitamin E-deficiency syndromes and that formation of free iron is the first event of oxidative tissue damage.

강박증 환자에서의 인격장애 공존병리에 관한 연구

이동우,강웅구,류인균,정도언,조맹제,우종인,김용식,권준수 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.3

연구목적 : 강박증의 동반인격장애에 관한 연구들은 기존의 개념과 달리 강박성 인격장애와 강박증 간에는 특이적인 관계가 없으며, 강박증 환자들은 다양한 인격장애를 동반한다는 결과를 보여주었다. 그러나 이러한 연구들은 동반 인격장애에 관한 기술적인 분석에 그쳤고, 강박증 환자군의 임상 증상과 동반 인격장애 양상간의 연관성에 관한 분석을 시행하지 않았다. 본 연구의 목적은 강박증 환자군에서의 동반 인격장애를 평가하고 강박증 환자의 다양한 증상과 동반 인격특성 간의 연관성에 대해 연구하는 것이다. 방 법 : DSM-IV의 강박증의 진단기준을 만족하는 59명의 환자군과 32명의 정상대조군을 대상으로 인격장애 질문지를 적용한 후 4∼6점의 절단점을 기준으로 인격장애의 진단이 내려졌다. 먼저 환자군과 정상대조군 간의 특정 인격장애 빈도의 차이를 알아보기 위하여 Fisher's exact test를 시행한 후, 두집단 간에 유의한 차이가 있는 것으로 밝혀진 인격장애 척도 점수에 미치는 Y-BOCS,BDI,BAI점수의 영향을 알아보기 위하여 다중선형회귀 분석을 시행하였다. 결 과 : 1) Fisher's exact test 결과 강박증 환자군은 정상 대조군에 비해 경계성 인격장애(18.6%vs 0%, p=0.007). 회피성 인격장애(55.9% vs 18.8%, p=0.001), 우울성 인격장애(40.7% vs 0%, p=0.000)의 비율이 유의하게 높았다. 2) 다중선형회귀분석 결과 Y-BOCS 점수 (t=2.10, p=0.043)와 BAI점수(t=2.60, p=0.014)는 경계성 인격장애 점수에 유의한 영향을 주는 것으로 나타났다. 또한 BAI점수(t=3.23, p<0.003)는 회피성 인격장애 저수에, BDI점수 (t=3.08, p=0.004)는 우울성 인격장애 점수에 유의한 영향을 주는 것으로 나타났다. 결 론 : 강박증 환자군은 정상 대조군에 비해 회피성 인격장애, 우울성 인격장애, 경계성 인격장애의 동반율이 높고, 강박증이 경계성 인격장애의 심각도에 유의한 영향을 미치는 것으로 사료된다. The purpose of this study was to assess the comorbid axis-Ⅱ disorders of obsessive compulsive disorder(OCD) patients and to investigate the relationship between symptoms of OCD and the comorbid personality traits. The subjects were 59 patients who met DSM-IV criteria for obsessive-compulsive disorder and 32 normal controls. All subjects completed personality Disorder Questionnaire-IV(PDQ-IV). The patients completed Beck Depression Inventory(BDI) and Beck Anxiety Inventory(BAI), and were rated with Yale-Brown Obsessive Compulsive Scale(YBOCS). The results were as follows. 1) The OCD patients showed significantly higher prevalence of avoidant, depresssive and borderline personality disorder(p<0.01) compared to controls. 2) The BAI score had significant effect on the avoidant personality score(t=3,23, p<0.003). The BDI score had significant effect on the depressive personality score(t=3.08, p=0.004). The YBOCS(t=2.10, p=0.043) and BAI(t=2.60, p=0.014) scores had significant effects on the borderline personality score. We found that OCD patients had higher prevalence of avoidant, depressive, and borderline personality disorders. We also found that obsessive-compulsive symptoms have significant effect on the severity of borderline personalty traits. We suggest that it would be very helpful to consider Axis-Ⅱ disorders for managing patients with obsessive-compulsive disorder.

Rat의 간세포 항산화계 및 지질 과산화에 미치는 Mycotoxins의 영향 : I. Effects of Fumonisin B₁, a Mycotoxin produced by Fusarium moniliforme I. Fumonisin B₁의 영향

李雄洙 忠州大學校 1994 한국교통대학교 논문집 Vol.29 No.2

The purpose of this study was to determine if fumonisin B₁(FB₁), a promoter for hepa tocarcinogenesis in rat, induces lipid peroxidation and alters the concentrations of antioxidant systems in rat liver cell. The hepatocytes were isolated from male Harlan Sprague-Dawley rat by using aseptic procedures, and treated with varying concentrations of FB₁(1μM,10μM and 50μM). The levels of malondialdehyde(MDA), conjugated diene(CD), vitamin A(V-A), vitamin E(V-E),γ-tocopheryl quinone(γ-TQ), vitaminC(V-C), glutathione(GSH), total lipids(TL), and protein were determined to assess oxidative damage and antioxidant status on the hepatic cell. FB₁ increased the levels of MDA and CD, oxidative products of cellular lipid. And FB₁ decreased the levels of cellular antioxdants such as V-E, and V-A, and V-C, but increased GSH level. This study shows that the adiministration of FB₁, a mycotoxin produced by Fusarium moniliforme, did increase indices of lipid peroxidation, but that cellular antioxidant defences were inhibited by FB₁.